BMB Reports最新文献_第9页

Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer 作为双重角色生物标记物的钙粘连蛋白：肺癌的抗体诊断和靶向治疗

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-05 DOI: 10.5483/BMBRep.2023-0228

Soyeon Lim, Youngeun Ha, Boram Lee, Junho Shin, Taiyoun Rhim

{"title":"Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer","authors":"Soyeon Lim, Youngeun Ha, Boram Lee, Junho Shin, Taiyoun Rhim","doi":"10.5483/BMBRep.2023-0228","DOIUrl":"https://doi.org/10.5483/BMBRep.2023-0228","url":null,"abstract":"Lung cancer carries one of the highest mortality rates among all cancers. It is often diagnosed at more advanced stages with limited treatment options compared to other malignancies. This study focuses on calnexin as a potential biomarker for diagnosis and treatment of lung cancer. Calnexin, a molecular chaperone integral to N-linked glycoprotein synthesis, has shown some associations with cancer. However, targeted therapeutic or diagnostic methods using calnexin have been proposed. Through 1D-LCMSMS, we identified calnexin as a biomarker for lung cancer and substantiated its expression in human lung cancer cell membranes using Western blotting, flow cytometry, and immunocytochemistry. Anti-calnexin antibodies exhibited complement-dependent cytotoxicity to lung cancer cell lines, resulting in a notable reduction in tumor growth in a subcutaneous xenograft model. Additionally, we verified the feasibility of labeling tumors through in vivo imaging using antibodies against calnexin. Furthermore, exosomal detection of calnexin suggested the potential utility of liquid biopsy for diagnostic purposes. In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140461771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway. 葡萄糖胺通过调节哺乳动物雷帕霉素信号通路靶点增加巨噬细胞脂质积累。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Sang-Min Kim, Dong Yeol Kim, Jiwon Park, Young-Ah Moon, Inn-Oc Han

{"title":"Glucosamine increases macrophage lipid accumulation by regulating the mammalian target of rapamycin signaling pathway.","authors":"Sang-Min Kim, Dong Yeol Kim, Jiwon Park, Young-Ah Moon, Inn-Oc Han","doi":"","DOIUrl":"","url":null,"abstract":"Elevated blood glucose is associated with an increased risk of atherosclerosis. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although there was no a significant change in the rate of fatty acid uptake. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, liver X receptor, and sterol regulatory elementbinding protein-1c (SREBP-1c) mRNA expression, and; conversely, suppressed ATP-binding cassette transporter A1 (ABCA-1) and ABCG-1 expression. Additionally, GlcN promoted O-GlcNAcylation of nuclear SREBP-1 but did not affect its DNA binding activity. GlcN stimulated phosphorylation of mammalian target of rapamycin (mTOR) and S6 kinase. Rapamycin, a mTOR-specific inhibitor, suppressed GlcN-induced lipid accumulation in RAW264.7 cells. The GlcN-mediated increase in ACC and FAS mRNA was suppressed, while the decrease in ABCA-1 and ABCG-1 by GlcN was not significantly altered by rapamycin. Together, our results highlight the importance of the mTOR signaling pathway in GlcN-induced macrophage lipid accumulation and further support a potential link between mTOR and HBP signaling in lipogenesis. [BMB Reports 2024; 57(2): 92-97].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDAT1 genome editing reduces hydroxy fatty acid production in transgenic Arabidopsis. PDAT1 基因组编辑减少了转基因拟南芥中羟基脂肪酸的产生。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Mid-Eum Park, Hyun Uk Kim

{"title":"PDAT1 genome editing reduces hydroxy fatty acid production in transgenic Arabidopsis.","authors":"Mid-Eum Park, Hyun Uk Kim","doi":"","DOIUrl":"","url":null,"abstract":"The fatty acids content of castor (Ricinus communis L.) seed oil is 80-90% ricinoleic acid, which is a hydroxy fatty acid (HFA). The structures and functional groups of HFAs are different from those of common fatty acids and are useful for various industrial applications. However, castor seeds contain the toxin ricin and an allergenic protein, which limit their cultivation. Accordingly, many researchers are conducting studies to enhance the production of HFAs in Arabidopsis thaliana, a model plant for oil crops. Oleate 12-hydroxylase from castor (RcFAH12), which synthesizes HFA (18:1-OH), was transformed into an Arabidopsis fae1 mutant, resulting in the CL37 line producing a maximum of 17% HFA content. In addition, castor phospholipid:diacylglycerol acyltransferase 1-2 (RcPDAT1-2), which catalyzes the production of triacylglycerol by transferring HFA from phosphatidylcholine to diacylglycerol, was transformed into the CL37 line to develop a P327 line that produces 25% HFA. In this study, we investigated changes in HFA content when endogenous Arabidopsis PDAT1 (AtPDAT1) of the P327 line was edited using the CRISPR/Cas9 technique. The successful mutation resulted in three independent lines with different mutation patterns, which were transmitted until the T4 generation. Fatty acid analysis of the seeds showed that HFA content decreased in all three mutant lines. These findings indicate that AtPDAT1 as well as RcPDAT1-2 in the P327 line are involved in transferring and increasing HFAs to triacylglycerol. [BMB Reports 2024; 57(2): 86-91].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138486671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: circRNA circSnx12 confers Cisplatin chemoresistance to ovarian cancer by inhibiting ferroptosis through a miR-194-5p/SLC7A11 axis. 勘误：circRNA circSnx12通过miR-194-5p/SLC7A11轴抑制铁突变，从而赋予卵巢癌顺铂化疗耐药性。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Kaiyun Qin, Fenghua Zhang, Hongxia Wang, Na Wang, Hongbing Qiu, Xinzhuan Jia, Shan Gong, Zhengmao Zhang

引用次数: 0

Retraction: Inhibition of ClC-5 suppresses proliferation and induces apoptosis in cholangiocarcinoma cells through the Wnt/β-catenin signaling pathway. 撤回：抑制 ClC-5 可通过 Wnt/β-catenin 信号通路抑制胆管癌细胞的增殖并诱导其凋亡。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Zhe Shi, Liyuan Zhou, Yan Zhou, Xiaoyan Jia, Xiangjun Yu, Xiaohong An, Yanzhen Han

引用次数: 0

Research progress on hydrogel-based drug therapy in melanoma immunotherapy. 水凝胶药物疗法在黑色素瘤免疫疗法中的研究进展。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Wei He, Yanqin Zhang, Yi Qu, Mengmeng Liu, Guodong Li, Luxiang Pan, Xinyao Xu, Gege Shi, Qiang Hao, Fen Liu, Yuan Gao

{"title":"Research progress on hydrogel-based drug therapy in melanoma immunotherapy.","authors":"Wei He, Yanqin Zhang, Yi Qu, Mengmeng Liu, Guodong Li, Luxiang Pan, Xinyao Xu, Gege Shi, Qiang Hao, Fen Liu, Yuan Gao","doi":"","DOIUrl":"","url":null,"abstract":"Melanoma is one of the most aggressive skin tumors, and conventional treatment modalities are not effective in treating advanced melanoma. Although immunotherapy is an effective treatment for melanoma, it has disadvantages, such as a poor response rate and serious systemic immune-related toxic side effects. The main solution to this problem is the use of biological materials such as hydrogels to reduce these side effects and amplify the immune killing effect against tumor cells. Hydrogels have great advantages as local slow-release drug carriers, including the ability to deliver antitumor drugs directly to the tumor site, enhance the local drug concentration in tumor tissue, reduce systemic drug distribution and exhibit good degradability. Despite these advantages, there has been limited research on the application of hydrogels in melanoma treatment. Therefore, this article provides a comprehensive review of the potential application of hydrogels in melanoma immunotherapy. Hydrogels can serve as carriers for sustained drug delivery, enabling the targeted and localized delivery of drugs with minimal systemic side effects. This approach has the potential to improve the efficacy of immunotherapy for melanoma. Thus, the use of hydrogels as drug delivery vehicles for melanoma immunotherapy has great potential and warrants further exploration. [BMB Reports 2024; 57(2): 71-78].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138486674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair. 肝脏 Sirt7 的缺失会损害 DNA 损伤修复，从而加速二乙基亚硝胺（DEN）诱导的肝细胞癌的形成。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Yuna Kim, Baeki E Kang, Karim Gariani, Joanna Gariani, Junguee Lee, Hyun-Jin Kim, Chang-Woo Lee, Kristina Schoonjans, Johan Auwerx, Dongryeol Ryu

{"title":"Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair.","authors":"Yuna Kim, Baeki E Kang, Karim Gariani, Joanna Gariani, Junguee Lee, Hyun-Jin Kim, Chang-Woo Lee, Kristina Schoonjans, Johan Auwerx, Dongryeol Ryu","doi":"","DOIUrl":"","url":null,"abstract":"The mammalian sirtuin family (SIRT1-SIRT7) has shown diverse biological roles in the regulation and maintenance of genome stability under genotoxic stress. SIRT7, one of the least studied sirtuin, has been demonstrated to be a key factor for DNA damage response (DDR). However, conflicting results have proposed that Sirt7 is an oncogenic factor to promote transformation in cancer cells. To address this inconsistency, we investigated properties of SIRT7 in hepatocellular carcinoma (HCC) regulation under DNA damage and found that loss of hepatic Sirt7 accelerated HCC progression. Specifically, the number, size, and volume of hepatic tumor colonies in diethylnitrosamine (DEN) injected Sirt7-deficient liver were markedly enhanced. Further, levels of HCC progression markers and pro-inflammatory cytokines were significantly elevated in the absence of hepatic Sirt7, unlike those in the control. In chromatin, SIRT7 was stabilized and colocalized to damage site by inhibiting the induction of γH2AX under DNA damage. Together, our findings suggest that SIRT7 is a crucial factor for DNA damage repair and that hepatic loss-of-Sirt7 can promote genomic instability and accelerate HCC development, unlike early studies describing that Sirt7 is an oncogenic factor [BMB Reports 2024; 57(2): 98-103].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice. 通过 CRISPR/Cas9 基因病毒复制子系统生成的水稻收获前萌芽抗性突变体的表型特征。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Jong Hee Kim, Jihyeon Yu, Jin Young Kim, Yong Jin Park, Sangsu Bae, Kwon Kyoo Kang, Yu Jin Jung

{"title":"Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice.","authors":"Jong Hee Kim, Jihyeon Yu, Jin Young Kim, Yong Jin Park, Sangsu Bae, Kwon Kyoo Kang, Yu Jin Jung","doi":"","DOIUrl":"","url":null,"abstract":"Pre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T1 seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiological properties, and biochemical properties of target genes in the ERF1-hdr line were investigated for three years. The ERF1-hdr line showed significantly enhanced seed dormancy and pre-harvest sprouting resistance. qRT-PCR analysis suggested that enhanced ABA signaling resulted in a stronger phenotype of PHS resistance. These results indicate that efficient HDR can be achieved through SNP/InDel replacement using a single and modular configuration applicable to different rice targets and other crops. This work demonstrates the potential to replace all genes with elite alleles within one generation and greatly expands our ability to improve agriculturally important traits. [BMB Reports 2024; 57(2): 79-85].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer. 韩国结直肠癌患者的 DNA 甲基化综合分析。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Hyeran Shim, Kiwon Jang, Yeong Hak Bang, Hoang Bao Khanh Chu, Jisun Kang, Jin-Young Lee, Sheehyun Cho, Hong Seok Lee, Jongbum Jeon, Taeyeon Hwang, Soobok Joe, Jinyeong Lim, Ji-Hye Choi, Eun Hye Joo, Kyunghee Park, Ji Hwan Moon, Kyung Yeon Han, Yourae Hong, Woo Yong Lee, Hee Cheol Kim, Seong Hyeon Yun, Yong Beom Cho, Yoon Ah Park, Jung Wook Huh, Jung Kyong Shin, Dae Hee Pyo, Hyekyung Hong, Hae-Ock Lee, Woong-Yang Park, Jin Ok Yang, Young-Joon Kim

{"title":"Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer.","authors":"Hyeran Shim, Kiwon Jang, Yeong Hak Bang, Hoang Bao Khanh Chu, Jisun Kang, Jin-Young Lee, Sheehyun Cho, Hong Seok Lee, Jongbum Jeon, Taeyeon Hwang, Soobok Joe, Jinyeong Lim, Ji-Hye Choi, Eun Hye Joo, Kyunghee Park, Ji Hwan Moon, Kyung Yeon Han, Yourae Hong, Woo Yong Lee, Hee Cheol Kim, Seong Hyeon Yun, Yong Beom Cho, Yoon Ah Park, Jung Wook Huh, Jung Kyong Shin, Dae Hee Pyo, Hyekyung Hong, Hae-Ock Lee, Woong-Yang Park, Jin Ok Yang, Young-Joon Kim","doi":"","DOIUrl":"","url":null,"abstract":"Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator PhenotypeHigh (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the thylation patterns and clinical characteristics of Korean patients with CRC. This valuable dataset lays a strong foundation for exploring novel molecular insights and potential therapeutic targets for the treatment of CRC. [BMB Reports 2024; 57(2): 110-115].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10414456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line. 在使用 RPTEC/TERT1 细胞系的肾脏纤维化体外模型中，BMSC 调节培养基的治疗潜力。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-02-01

Yunji Kim, Dayeon Kang, Ga-Eun Choi, Sang Dae Kim, Sun-Ja Yang, Hyosang Kim, Dalsan You, Choung Soo Kim, Nayoung Suh

{"title":"Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line.","authors":"Yunji Kim, Dayeon Kang, Ga-Eun Choi, Sang Dae Kim, Sun-Ja Yang, Hyosang Kim, Dalsan You, Choung Soo Kim, Nayoung Suh","doi":"","DOIUrl":"","url":null,"abstract":"We investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibrotic markers (α-SMA and vimentin) and increases in the levels of the epithelial marker E-cadherin and aquaporin 7, particularly under stiff conditions. Furthermore, BMSC-CM modified microRNA (miRNA) expression and reduced oxidative stress levels in these cells. Our findings suggest that BMSC-CM can modulate cellular morphology, miRNA expression, and oxidative stress in RPTEC/TERT1 cells, highlighting its therapeutic potential in fibrotic kidney disease. [BMB Reports 2024; 57(2): 116-121].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10910087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0