Biologia最新文献_第8页

Comparative protein structural analyses of α-synuclein linked pathogenic variants reveal the role of N-terminally located critical region in Parkinson’s disease pathogenesis α-突触核蛋白相关致病变体的蛋白质结构比较分析揭示了位于 N 端的关键区域在帕金森病发病机制中的作用

IF 1.5 4区生物学

Biologia Pub Date : 2024-06-11 DOI: 10.1007/s11756-024-01713-6

Kashifa Akhtar Mughal, Aiman Khan, Amina Sarwar, Rida Mahmood, Amir Ali Abbasi

引用次数: 0

In vitro evaluation of probiotic and antioxidant potential of Lacticaseibacillus paracasei ED25 副乳酸杆菌 ED25 的益生菌和抗氧化潜力的体外评估

IF 1.5 4区生物学

Biologia Pub Date : 2024-06-11 DOI: 10.1007/s11756-024-01720-7

Elanur Dasdemir, N. Arslan, Serkan Ortucu, Gurkan Aykutoglu, Hakan Ozkan, A. Adiguzel, Mesut Taskin

引用次数: 0

Antimicrobial and antioxidant activity and GC-MS metabolite profiling of cyanobacteria in the tersakan stream (Amasya, Türkiye) 特尔萨坎溪流（土耳其阿马西亚）中蓝藻的抗菌和抗氧化活性及 GC-MS 代谢物谱分析

IF 1.5 4区生物学

Biologia Pub Date : 2024-06-10 DOI: 10.1007/s11756-024-01706-5

E. Çelikoğlu, M. Y. Cankılıç, Ö. İdil

引用次数: 0

Development of the dynamic approach for detection of Salmonella Typhi biofilm via optimizations of interaction with safranin dye 通过优化与黄樟素染料的相互作用，开发检测伤寒沙门氏菌生物膜的动态方法

IF 1.5 4区生物学

Biologia Pub Date : 2024-06-04 DOI: 10.1007/s11756-024-01716-3

Aditya Upadhyay, Dharm Pal, Awanish Kumar

{"title":"Development of the dynamic approach for detection of Salmonella Typhi biofilm via optimizations of interaction with safranin dye","authors":"Aditya Upadhyay, Dharm Pal, Awanish Kumar","doi":"10.1007/s11756-024-01716-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01716-3","url":null,"abstract":"Salmonella Typhi infection is showing as a negative alarm for human health due to the occurrence of life-threatening typhoid fever and the emergence of antibiotic resistance. The infection becomes chronic due to the biofilm formation of S. Typhi, which plays a significant role in antibiotic resistance. The S. Typhi can survive under various stress conditions such as antibiotic stress, host immune stress, environmental stress, and nutritional stress in the biofilm stage. Biofilm provides a protective cover to the S. Typhi and prevents the entry of antibiotics inside the bacterial cells. Thus, biofilm is the root cause of the problem. Therefore, this study explores the easy detection of S. Typhi biofilms using safranin dye with specific concentrations. We performed the biofilm growth kinetics, qualitative assessment of the biofilm formations, and safranin interactions to finalize the specific concentrations of safranin (0.5 mg/mL) to detect the S. Typhi biofilm condition. The study was validated at 0.5 mg/mL safranin concentrations with the help of RSM analysis and kinetics study of the typhoidal biofilm. The study is important because the obtained results indicated the precise detection of S. Typhi biofilm, which is useful for all phases of biofilm detection. The study provided new directions for typhoidal biofilm staining that could be an emerging methodology in the area of health sciences.","PeriodicalId":8978,"journal":{"name":"Biologia","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141255335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia 斯洛伐克与伏甲 Xylosandrus germanus 相关的真菌

IF 1.5 4区生物学

Biologia Pub Date : 2024-06-04 DOI: 10.1007/s11756-024-01712-7

Katarína Pastirčáková, Katarína Baková, Katarína Adamčíková, Marek Barta, Michal Lalík, Martin Pavlík, Andrej Kunca, Juraj Galko, Martin Pastirčák

引用次数: 0

Production of N-glycosylated alcohol dehydrogenase in Escherichia coli 在大肠杆菌中生产 N-糖基化醇脱氢酶

IF 1.5 4区生物学

Biologia Pub Date : 2024-05-31 DOI: 10.1007/s11756-024-01707-4

Zdenko Levarski, Stanislava Bírová, Kristina Hriňová, Johana Dlapová, Eva Struhárňanská, Lenka Levarská, Ján Turňa, Stanislav Stuchlík

{"title":"Production of N-glycosylated alcohol dehydrogenase in Escherichia coli","authors":"Zdenko Levarski, Stanislava Bírová, Kristina Hriňová, Johana Dlapová, Eva Struhárňanská, Lenka Levarská, Ján Turňa, Stanislav Stuchlík","doi":"10.1007/s11756-024-01707-4","DOIUrl":"https://doi.org/10.1007/s11756-024-01707-4","url":null,"abstract":"N-glycosylation of recombinant proteins using bacterial glycosylation system has proven to be a valuable although developing tool ultimately applicable to various industries. When used for enzyme engineering, it offers the possibility of increased stability or immobilization route and thus increasing effectiveness of e.g. biotransformation or other biocatalysis procedures. One such promising enzyme is alcohol dehydrogenase (ADH) for use in redox biotransformation reactions. Given the current possibilities of recombinant enzyme production, including major advances in glycoengineering and glycoprotein production in bacterial organisms, the aim of this work was the production of thermotolerant ADH from Rhodococcus ruber (RrADH) in glycosylated form in Escherichia coli. We have successfully developed a dual plasmid expression system enabling glycosylation of target proteins utilizing a glyco-tag approach. We were able to produce RrADH in soluble form and at the same time we detected a bacterial glycan conjugated to RrADH as well as the activity of the enzyme. The glycan bound to recombinant enzyme can be used for oriented covalent immobilization of the enzyme, which would increase the potential for its practical application in biotransformation of various compounds.","PeriodicalId":8978,"journal":{"name":"Biologia","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico analysis of a heme-thiolate peroxidase gene discovered in an ectomycorrhizal fungus of Carpathian primeval forest: implications for biotechnological applications 对喀尔巴阡山原始森林一种外生菌根真菌中发现的血红硫酸盐过氧化物酶基因的硅学分析：对生物技术应用的影响

IF 1.5 4区生物学

Biologia Pub Date : 2024-05-30 DOI: 10.1007/s11756-024-01709-2

Bohuš Kubala, Peter Ferianc, Katarína Chovanová, Marcel Zámocký

{"title":"In silico analysis of a heme-thiolate peroxidase gene discovered in an ectomycorrhizal fungus of Carpathian primeval forest: implications for biotechnological applications","authors":"Bohuš Kubala, Peter Ferianc, Katarína Chovanová, Marcel Zámocký","doi":"10.1007/s11756-024-01709-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01709-2","url":null,"abstract":" In this work, we focus on the identification of novel fungal peroxygenase gene belonging to the peroxidase-peroxygenase superfamily. We applied a metagenomic approach on soil samples from primeval forest and appropriate bioinformatics tools for analysis of obtained genomic DNA sequence. Peroxidases are ubiquitous metalloenzymes that are able to reduce reactive peroxides, mainly hydrogen peroxide, into water, whereas several substrates can be concomitantly oxidized during their catalytic reaction. Our purpose was to collect unique peroxygenase sequence data originating from a preserved biotope for a robust phylogenetic reconstruction of a particular gene family coding for highly versatile heme-thiolate peroxidases that has peculiar yet undiscovered representatives among ectomycorrhizal fungi. We identified unique DNA sequence, 812 bp long, from ectomycorrhizal Suillus species coding for a heme-thiolate peroxidase with 1 typical intron that appears distinctive for Carpathian forests. After translation in corresponding protein sequence 251 amino acids long we could identify typical signatures of this peroxygenase. On the proximal side of heme we found the conserved P-C-P triad responsible for efficient ligation of heme iron thus influencing the reactivity of this peroxidase. On the distal side we recognized the E-H-D-X-S-L motif for interaction with a stabilizing magnesium ion. Maximum likelihood reconstruction of protein phylogeny revealed with a high bootstrap support the presence of a monophyletic HTP4 clade originating in numerous Suillus representatives. Together with sister clades of edible Boletus and poisonous Paxillus containing diverse peroxygenases these newly discovered heme catalyst can be considered for application of oxyfunctionalization of organic molecules.","PeriodicalId":8978,"journal":{"name":"Biologia","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biostimulatory effects of ascorbic acid in improving plant growth, photosynthesis-related parameters and mitigating oxidative damage in alfalfa (Medicago sativa L.) under salt stress condition 抗坏血酸在改善盐胁迫条件下紫花苜蓿（Medicago sativa L.）的植物生长、光合作用相关参数和减轻氧化损伤方面的生物刺激作用

IF 1.5 4区生物学

Biologia Pub Date : 2024-05-27 DOI: 10.1007/s11756-024-01704-7

Ahmed El Moukhtari, Nadia Lamsaadi, Mohamed Farissi

{"title":"Biostimulatory effects of ascorbic acid in improving plant growth, photosynthesis-related parameters and mitigating oxidative damage in alfalfa (Medicago sativa L.) under salt stress condition","authors":"Ahmed El Moukhtari, Nadia Lamsaadi, Mohamed Farissi","doi":"10.1007/s11756-024-01704-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01704-7","url":null,"abstract":"Biostimulants such as ascorbic acid, known as vitamin C, have been reported to have numerous positive roles in plant tolerance to abiotic stresses. However, little is known about the biostimulant effects of ascorbic acid on alfalfa (Medicago sativa). Accordingly, a pot experiment was conducted to investigate the effects of 1 mM ascorbic acid, applied as foliar spray, on the salt tolerance of a Moroccan alfalfa population Demnate 201. One month-old M. sativa seedlings were exposed to 200 mM NaCl for four weeks with or without 1 mM of exogenous ascorbic acid treatment. The results showed that salinity stress significantly (p < 0.001) reduced plant biomass, disturbed photosynthesis-related parameters and induced oxidative stress. However, ascorbic acid foliar spray counteracted the observed negative effects of salinity. It significantly (p < 0.001) improved plant growth and photosynthetic parameters. Besides, stress indicators, including Na+ in shoot and root, hydrogen peroxide and electrolyte leakage, were significantly reduced by 42%, 29%, 12% and 34%, respectively, in treated and salt-stressed alfalfa plants. Interestingly, the decrease in oxidative stress markers was positively correlated to the ability of ascorbic acid to induce the accumulation of flavonoids and to increase the antioxidant activity of guaiacol peroxidase. Furthermore, compatible solutes, such as proline and soluble sugars, were found higher especially in salt-stressed alfalfa plants treated with 1 mM ascorbic acid. Our findings showed that ascorbic acid supply could be an eco-friendly and sustainable technique to mitigate the toxic effect of salt and could improve alfalfa forage production when grown in salt-affected soils.","PeriodicalId":8978,"journal":{"name":"Biologia","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenic and endosymbiotic bacteria associated with the ectoparasite mites Dermanyssus gallinae (Dermanyssidae) and Ornithonyssus bursa (Macronyssidae) 与外寄生螨 Dermanyssus gallinae（Dermanyssidae）和 Ornithonyssus bursa（Macronyssidae）相关的致病细菌和内共生细菌

IF 1.5 4区生物学

Biologia Pub Date : 2024-05-24 DOI: 10.1007/s11756-024-01708-3

Tainá Belleboni, R. Bassini-Silva, A. Sulzbach, Alana Luísa Scherer, Evelise Cargnin Trebien, Micaela Schneider, Francine Cesca, Pietra Lenz Kniphoff da Cruz, Ágatha Kniphoff da Cruz, Fernando de Castro Jacinavicius, Laura Marina Ohlweiler, Liana Johann, G. L. da Silva

引用次数: 0

Evolution and association analysis of SSIIIa in rice landraces of Yunnan Province 云南省水稻陆稻品种 SSIIIa 的进化与关联分析

IF 1.5 4区生物学

Biologia Pub Date : 2024-05-24 DOI: 10.1007/s11756-024-01705-6

Xia Li, Xiaomen Yang, Li’e Yang, Hafiz Ghulam Muhu-Din Ahmed, Chunlian Yao, Jiazhen Yang, Luxiang Wang, Tao Yang, Xiaoying Pu, Yawen Zeng

引用次数: 0