Biologia最新文献

{"title":"First insights into the complete mitochondrial genome of a deep-sea eel Congriscus megastoma (Anguilliformes, Congroidei, Congridae): characterization and phylogenetic inference","authors":"Yuping Liu, Peiyi Xiao, Tianyan Yang","doi":"10.1007/s11756-024-01718-1","DOIUrl":"https://doi.org/10.1007/s11756-024-01718-1","url":null,"abstract":"<p>The complete mitochondrial DNA sequence of a deep-sea eel <i>Congriscus megastoma</i> (Anguilliformes: Congridae) was determined by high throughput sequencing technology. As the first mitogenome of the genus <i>Congriscus</i> up to now, its entire mtDNA sequence was 17,616 bp in length, including 37 mitochondrial genes (13 protein-coding genes, 2 rRNA genes, 22 tRNA genes) and 2 control regions, which was inconsistent with that of the typical vertebrate mitogenomes. In addition, the genes of <i>C. megastoma</i> exhibited an obvious rearrangement. <i>ND6</i> and tRNA-Glu were transferred between tRNA-Thr and tRNA-Pro, as well as a doubled control region appeared. The tandem duplication-random loss (TDRL) model was an ideal explanation for this irregular gene reorganization phenomenon. The Bayesian phylogenetic tree showed that Congridae species were divided into four clades, suggesting their non-monophyletic origin. <i>Congriscus</i> had the closest relationships with <i>Thalassenchelys</i> and <i>Coloconger</i>. Species with gene rearrangement separately clustered as one clade. The results provided a basis for future studies of mitochondrial DNA structure and phylogeny of Anguilliformes.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"172 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of the dynamic approach for detection of Salmonella Typhi biofilm via optimizations of interaction with safranin dye","authors":"Aditya Upadhyay, Dharm Pal, Awanish Kumar","doi":"10.1007/s11756-024-01716-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01716-3","url":null,"abstract":"<p><i>Salmonella</i> Typhi infection is showing as a negative alarm for human health due to the occurrence of life-threatening typhoid fever and the emergence of antibiotic resistance. The infection becomes chronic due to the biofilm formation of <i>S.</i> Typhi, which plays a significant role in antibiotic resistance. The <i>S.</i> Typhi can survive under various stress conditions such as antibiotic stress, host immune stress, environmental stress, and nutritional stress in the biofilm stage. Biofilm provides a protective cover to the <i>S.</i> Typhi and prevents the entry of antibiotics inside the bacterial cells. Thus, biofilm is the root cause of the problem. Therefore, this study explores the easy detection of <i>S.</i> Typhi biofilms using safranin dye with specific concentrations. We performed the biofilm growth kinetics, qualitative assessment of the biofilm formations, and safranin interactions to finalize the specific concentrations of safranin (0.5 mg/mL) to detect the <i>S.</i> Typhi biofilm condition. The study was validated at 0.5 mg/mL safranin concentrations with the help of RSM analysis and kinetics study of the typhoidal biofilm. The study is important because the obtained results indicated the precise detection of <i>S.</i> Typhi biofilm, which is useful for all phases of biofilm detection. The study provided new directions for typhoidal biofilm staining that could be an emerging methodology in the area of health sciences.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"21 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141255335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia","authors":"Katarína Pastirčáková, Katarína Baková, Katarína Adamčíková, Marek Barta, Michal Lalík, Martin Pavlík, Andrej Kunca, Juraj Galko, Martin Pastirčák","doi":"10.1007/s11756-024-01712-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01712-7","url":null,"abstract":"<p>The black timber bark beetle (<i>Xylosandrus germanus</i>) is a strongly invasive ambrosia beetle and an important forest pest in Slovakia. This pest is closely associated with symbiotic fungi used as its food source. We investigated the fungi associated with <i>X. germanus</i> adults in Slovakia. In this study, <i>Beauveria bassiana</i>, <i>B. pseudobassiana</i>, <i>Clonostachys rosea</i>, <i>Fusarium oxysporum</i>, <i>Ophiostoma quercus</i>, <i>Phaeoacremonium scolyti</i>, and <i>Talaromyces amestolkiae</i> were isolated and identified by morphological and molecular analyses. The fungus <i>Ophiostoma quercus</i> was most frequently isolated from living beetles, while the entomopathogenic <i>Beauveria bassiana</i> was the most commonly isolated from dead beetles. The morphological descriptions of fungi based on isolates from the surface of <i>X. germanus</i> adults are provided.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"19 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141256797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of N-glycosylated alcohol dehydrogenase in Escherichia coli","authors":"Zdenko Levarski, Stanislava Bírová, Kristina Hriňová, Johana Dlapová, Eva Struhárňanská, Lenka Levarská, Ján Turňa, Stanislav Stuchlík","doi":"10.1007/s11756-024-01707-4","DOIUrl":"https://doi.org/10.1007/s11756-024-01707-4","url":null,"abstract":"<p>N-glycosylation of recombinant proteins using bacterial glycosylation system has proven to be a valuable although developing tool ultimately applicable to various industries. When used for enzyme engineering, it offers the possibility of increased stability or immobilization route and thus increasing effectiveness of e.g. biotransformation or other biocatalysis procedures. One such promising enzyme is alcohol dehydrogenase (ADH) for use in redox biotransformation reactions. Given the current possibilities of recombinant enzyme production, including major advances in glycoengineering and glycoprotein production in bacterial organisms, the aim of this work was the production of thermotolerant ADH from <i>Rhodococcus ruber</i> (RrADH) in glycosylated form in <i>Escherichia coli</i>. We have successfully developed a dual plasmid expression system enabling glycosylation of target proteins utilizing a glyco-tag approach. We were able to produce RrADH in soluble form and at the same time we detected a bacterial glycan conjugated to RrADH as well as the activity of the enzyme. The glycan bound to recombinant enzyme can be used for oriented covalent immobilization of the enzyme, which would increase the potential for its practical application in biotransformation of various compounds.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"59 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico analysis of a heme-thiolate peroxidase gene discovered in an ectomycorrhizal fungus of Carpathian primeval forest: implications for biotechnological applications","authors":"Bohuš Kubala, Peter Ferianc, Katarína Chovanová, Marcel Zámocký","doi":"10.1007/s11756-024-01709-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01709-2","url":null,"abstract":"<p> In this work, we focus on the identification of novel fungal peroxygenase gene belonging to the peroxidase-peroxygenase superfamily. We applied a metagenomic approach on soil samples from primeval forest and appropriate bioinformatics tools for analysis of obtained genomic DNA sequence. Peroxidases are ubiquitous metalloenzymes that are able to reduce reactive peroxides, mainly hydrogen peroxide, into water, whereas several substrates can be concomitantly oxidized during their catalytic reaction. Our purpose was to collect unique peroxygenase sequence data originating from a preserved biotope for a robust phylogenetic reconstruction of a particular gene family coding for highly versatile heme-thiolate peroxidases that has peculiar yet undiscovered representatives among ectomycorrhizal fungi. We identified unique DNA sequence, 812 bp long, from ectomycorrhizal <i>Suillus</i> species coding for a heme-thiolate peroxidase with 1 typical intron that appears distinctive for Carpathian forests. After translation in corresponding protein sequence 251 amino acids long we could identify typical signatures of this peroxygenase. On the proximal side of heme we found the conserved P-C-P triad responsible for efficient ligation of heme iron thus influencing the reactivity of this peroxidase. On the distal side we recognized the E-H-D-X-S-L motif for interaction with a stabilizing magnesium ion. Maximum likelihood reconstruction of protein phylogeny revealed with a high bootstrap support the presence of a monophyletic HTP4 clade originating in numerous <i>Suillus</i> representatives. Together with sister clades of edible <i>Boletus</i> and poisonous <i>Paxillus</i> containing diverse peroxygenases these newly discovered heme catalyst can be considered for application of oxyfunctionalization of organic molecules.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"54 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biostimulatory effects of ascorbic acid in improving plant growth, photosynthesis-related parameters and mitigating oxidative damage in alfalfa (Medicago sativa L.) under salt stress condition","authors":"Ahmed El Moukhtari, Nadia Lamsaadi, Mohamed Farissi","doi":"10.1007/s11756-024-01704-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01704-7","url":null,"abstract":"<p>Biostimulants such as ascorbic acid, known as vitamin C, have been reported to have numerous positive roles in plant tolerance to abiotic stresses. However, little is known about the biostimulant effects of ascorbic acid on alfalfa (<i>Medicago sativa</i>). Accordingly, a pot experiment was conducted to investigate the effects of 1 mM ascorbic acid, applied as foliar spray, on the salt tolerance of a Moroccan alfalfa population <i>Demnate 201</i>. One month-old <i>M. sativa</i> seedlings were exposed to 200 mM NaCl for four weeks with or without 1 mM of exogenous ascorbic acid treatment. The results showed that salinity stress significantly (<i>p</i> &lt; 0.001) reduced plant biomass, disturbed photosynthesis-related parameters and induced oxidative stress. However, ascorbic acid foliar spray counteracted the observed negative effects of salinity. It significantly (<i>p</i> &lt; 0.001) improved plant growth and photosynthetic parameters. Besides, stress indicators, including Na⁺ in shoot and root, hydrogen peroxide and electrolyte leakage, were significantly reduced by 42%, 29%, 12% and 34%, respectively, in treated and salt-stressed alfalfa plants. Interestingly, the decrease in oxidative stress markers was positively correlated to the ability of ascorbic acid to induce the accumulation of flavonoids and to increase the antioxidant activity of guaiacol peroxidase. Furthermore, compatible solutes, such as proline and soluble sugars, were found higher especially in salt-stressed alfalfa plants treated with 1 mM ascorbic acid. Our findings showed that ascorbic acid supply could be an eco-friendly and sustainable technique to mitigate the toxic effect of salt and could improve alfalfa forage production when grown in salt-affected soils.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"51 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular diversity and antimicrobial susceptibility profile of Vibrio species and distribution of other bacteria isolated from water Hyacinth (Eichornia crassipes) and Lagos lagoon","authors":"Kinikanwo B. Wali, Utibeima U. Essiet, Abraham Ajayi, Grace Akintunde, Daniel K. Olukoya, Adeyemi I. Adeleye, Stella I. Smith","doi":"10.1007/s11756-024-01697-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01697-3","url":null,"abstract":"<p>Aquatic environments, including their flora and fauna, are known reservoirs of pathogenic <i>Vibrio</i> species. This study aimed to determine the diversity and antimicrobial susceptibility of bacteria from water hyacinth (<i>Eichornia crassipes</i>) and surrounding waters in Lagos lagoons. Bacteria isolation, phenotypic, and genotypic analysis were conducted using standard microbiology and molecular methods..Sampled lagoons exhibited physicochemical parameters such as dissolved oxygen (4.76—6.91 mgL⁻¹) and biochemical oxygen demand (80.5 – 242 mgL⁻¹) that exceeded permissible levels, indicating pollution. A total of 41 bacterial isolates were recovered from water hyacinth samples and surrounding lagoon waters. Twelve (29.26%) were <i>Vibrio</i> strains, comprising 4 (9.8%) <i>Vibrio cholerae</i>, 3 (7.3%) <i>V. paracholerae</i>, 2 (4.9%) <i>V. parahaemolyticus</i>, 2 (4.88%) <i>V. injensis</i> and 1 (2.4%) <i>V. mimicus</i>. Of the 4 V<i>. cholerae</i> isolates, 1 belonged to the O1 serotype. <i>Vibrio</i> species were exclusively recovered from water samples. Phylogenetic analysis of the <i>Vibrio</i> strains isolated from different lagoons showed genetic relatedness. All 41(100%) bacteria isolates displayed pan sensitivity to meropenem, while the highest resistance rate 27(65.9%) was recorded for amoxicillin + clavulanic acid. Multiple antibiotic resistance index of 8/12 (66.7%) of multidrug resistant <i>Vibrio</i> isolates was &gt; 0.2 (20%) which could imply a high risk source of contamination in the environment where they were isolated. <i>tdh</i>, <i>trh</i>, and <i>t1</i> genes were detected in <i>V. parahaemolyticus</i> isolates. Although, water hyacinth in this study did not harbored <i>Vibrio</i> species, the lagoon remains a source of pathogenic drug resistant <i>Vibrio</i> species. Therefore, surveillance should be enhanced.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"124 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140925862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ants (Hymenoptera: Formicidae) in subterranean natural cavities of Minas Gerais, Brazil","authors":"Natalia Ladino, Rodrigo M. Feitosa, Robson de Almeida Zampaulo, John E. Lattke","doi":"10.1007/s11756-024-01692-8","DOIUrl":"https://doi.org/10.1007/s11756-024-01692-8","url":null,"abstract":"<p>A list of ant species collected in caves at different localities of Minas Gerais state, Brazil is presented, based on specimens deposited in the Entomological Collection Padre Jesus Santiago Moure (DZUP). Of the 51 species/morphospecies found, two are new records for the state. We encourage an increase in ant collection efforts in Brazilian caves using different sampling techniques and comparative approaches in order to improve the current knowledge of ant diversity in these environments.\u0000</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"31 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140942335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)","authors":"Khadidja Meziane, Bruno Guinand, Mohamed Hichem Kara, Lamya Chaoui","doi":"10.1007/s11756-024-01683-9","DOIUrl":"https://doi.org/10.1007/s11756-024-01683-9","url":null,"abstract":"<p>Thin-lipped mullet (<i>Chelon ramada</i>) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"16 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-annual dynamics of stem circumference variation and water status of four coniferous tree species (Pinus sylvestris, Picea abies, Larix decidua and Abies alba) under warmer and water-limited conditions","authors":"Adriana Leštianska, Peter Fleischer, Katarína Merganičová, Peter Fleischer, Katarína Střelcová","doi":"10.1007/s11756-024-01693-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01693-7","url":null,"abstract":"<p>In the paper, the intra-annual growth patterns and tree water balance of four different tree species (<i>Pinus sylvestris</i>, <i>Picea abies</i>, <i>Larix decidua</i> and <i>Abies alba</i>) were examined. Seasonal radial increment, tree water deficit (ΔW) and maximum daily shrinkage (MDS) were derived from the records obtained using high-resolution digital band dendrometers. The study area was located in Arboretum Borová hora (350 m a. s. l., Zvolen valley, Central Slovakia) characterised by a warmer climate (<i>Picea abies</i>) and warmer and drier climate (<i>Abies alba</i>, <i>Larix decidua</i>, <i>Pinus sylvestris</i>) compared to the sites of tree origins. Monitored species exhibited remarkably distinct growth and water balance patterns over the 2015 growing season characterised with the highly above normal temperature and uneven precipitation distribution. <i>A. alba</i> exhibited smooth continuous growth least affected by varying environmental conditions. Of all analysed species, only <i>A. alba</i> showed significant positive correlations of radial growth and ΔW with temperature and global radiation, despite environmental water limitations. The lowest cumulative growth, lower negative values of ΔW and greater MDS of <i>L. decidua</i> indicate a higher water limitation of this species. The results showed more pronounced sensitivity of <i>P. sylvestris</i> to increased temperature and drought. All monitored variables of environmental conditions, except precipitation, significantly influenced MDS values of all studied tree species. Based on 30 variables describing radial stem growth patterns and water status we identified large inter-species variability and discrete species-specific groups, while the indicators of growth and water status of <i>L. decidua</i> and <i>P. sylvestris</i> were similar and the most different patterns were observed between <i>A. alba</i> and <i>L. decidua</i>. The behaviour of <i>P. abies</i> was closer to <i>A. alba</i> than to the other two species.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"109 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}