Biologia最新文献

{"title":"Biostimulatory effects of ascorbic acid in improving plant growth, photosynthesis-related parameters and mitigating oxidative damage in alfalfa (Medicago sativa L.) under salt stress condition","authors":"Ahmed El Moukhtari, Nadia Lamsaadi, Mohamed Farissi","doi":"10.1007/s11756-024-01704-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01704-7","url":null,"abstract":"<p>Biostimulants such as ascorbic acid, known as vitamin C, have been reported to have numerous positive roles in plant tolerance to abiotic stresses. However, little is known about the biostimulant effects of ascorbic acid on alfalfa (<i>Medicago sativa</i>). Accordingly, a pot experiment was conducted to investigate the effects of 1 mM ascorbic acid, applied as foliar spray, on the salt tolerance of a Moroccan alfalfa population <i>Demnate 201</i>. One month-old <i>M. sativa</i> seedlings were exposed to 200 mM NaCl for four weeks with or without 1 mM of exogenous ascorbic acid treatment. The results showed that salinity stress significantly (<i>p</i> &lt; 0.001) reduced plant biomass, disturbed photosynthesis-related parameters and induced oxidative stress. However, ascorbic acid foliar spray counteracted the observed negative effects of salinity. It significantly (<i>p</i> &lt; 0.001) improved plant growth and photosynthetic parameters. Besides, stress indicators, including Na⁺ in shoot and root, hydrogen peroxide and electrolyte leakage, were significantly reduced by 42%, 29%, 12% and 34%, respectively, in treated and salt-stressed alfalfa plants. Interestingly, the decrease in oxidative stress markers was positively correlated to the ability of ascorbic acid to induce the accumulation of flavonoids and to increase the antioxidant activity of guaiacol peroxidase. Furthermore, compatible solutes, such as proline and soluble sugars, were found higher especially in salt-stressed alfalfa plants treated with 1 mM ascorbic acid. Our findings showed that ascorbic acid supply could be an eco-friendly and sustainable technique to mitigate the toxic effect of salt and could improve alfalfa forage production when grown in salt-affected soils.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"51 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141167114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular diversity and antimicrobial susceptibility profile of Vibrio species and distribution of other bacteria isolated from water Hyacinth (Eichornia crassipes) and Lagos lagoon","authors":"Kinikanwo B. Wali, Utibeima U. Essiet, Abraham Ajayi, Grace Akintunde, Daniel K. Olukoya, Adeyemi I. Adeleye, Stella I. Smith","doi":"10.1007/s11756-024-01697-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01697-3","url":null,"abstract":"<p>Aquatic environments, including their flora and fauna, are known reservoirs of pathogenic <i>Vibrio</i> species. This study aimed to determine the diversity and antimicrobial susceptibility of bacteria from water hyacinth (<i>Eichornia crassipes</i>) and surrounding waters in Lagos lagoons. Bacteria isolation, phenotypic, and genotypic analysis were conducted using standard microbiology and molecular methods..Sampled lagoons exhibited physicochemical parameters such as dissolved oxygen (4.76—6.91 mgL⁻¹) and biochemical oxygen demand (80.5 – 242 mgL⁻¹) that exceeded permissible levels, indicating pollution. A total of 41 bacterial isolates were recovered from water hyacinth samples and surrounding lagoon waters. Twelve (29.26%) were <i>Vibrio</i> strains, comprising 4 (9.8%) <i>Vibrio cholerae</i>, 3 (7.3%) <i>V. paracholerae</i>, 2 (4.9%) <i>V. parahaemolyticus</i>, 2 (4.88%) <i>V. injensis</i> and 1 (2.4%) <i>V. mimicus</i>. Of the 4 V<i>. cholerae</i> isolates, 1 belonged to the O1 serotype. <i>Vibrio</i> species were exclusively recovered from water samples. Phylogenetic analysis of the <i>Vibrio</i> strains isolated from different lagoons showed genetic relatedness. All 41(100%) bacteria isolates displayed pan sensitivity to meropenem, while the highest resistance rate 27(65.9%) was recorded for amoxicillin + clavulanic acid. Multiple antibiotic resistance index of 8/12 (66.7%) of multidrug resistant <i>Vibrio</i> isolates was &gt; 0.2 (20%) which could imply a high risk source of contamination in the environment where they were isolated. <i>tdh</i>, <i>trh</i>, and <i>t1</i> genes were detected in <i>V. parahaemolyticus</i> isolates. Although, water hyacinth in this study did not harbored <i>Vibrio</i> species, the lagoon remains a source of pathogenic drug resistant <i>Vibrio</i> species. Therefore, surveillance should be enhanced.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"124 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140925862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ants (Hymenoptera: Formicidae) in subterranean natural cavities of Minas Gerais, Brazil","authors":"Natalia Ladino, Rodrigo M. Feitosa, Robson de Almeida Zampaulo, John E. Lattke","doi":"10.1007/s11756-024-01692-8","DOIUrl":"https://doi.org/10.1007/s11756-024-01692-8","url":null,"abstract":"<p>A list of ant species collected in caves at different localities of Minas Gerais state, Brazil is presented, based on specimens deposited in the Entomological Collection Padre Jesus Santiago Moure (DZUP). Of the 51 species/morphospecies found, two are new records for the state. We encourage an increase in ant collection efforts in Brazilian caves using different sampling techniques and comparative approaches in order to improve the current knowledge of ant diversity in these environments.\u0000</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"31 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140942335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)","authors":"Khadidja Meziane, Bruno Guinand, Mohamed Hichem Kara, Lamya Chaoui","doi":"10.1007/s11756-024-01683-9","DOIUrl":"https://doi.org/10.1007/s11756-024-01683-9","url":null,"abstract":"<p>Thin-lipped mullet (<i>Chelon ramada</i>) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"16 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-annual dynamics of stem circumference variation and water status of four coniferous tree species (Pinus sylvestris, Picea abies, Larix decidua and Abies alba) under warmer and water-limited conditions","authors":"Adriana Leštianska, Peter Fleischer, Katarína Merganičová, Peter Fleischer, Katarína Střelcová","doi":"10.1007/s11756-024-01693-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01693-7","url":null,"abstract":"<p>In the paper, the intra-annual growth patterns and tree water balance of four different tree species (<i>Pinus sylvestris</i>, <i>Picea abies</i>, <i>Larix decidua</i> and <i>Abies alba</i>) were examined. Seasonal radial increment, tree water deficit (ΔW) and maximum daily shrinkage (MDS) were derived from the records obtained using high-resolution digital band dendrometers. The study area was located in Arboretum Borová hora (350 m a. s. l., Zvolen valley, Central Slovakia) characterised by a warmer climate (<i>Picea abies</i>) and warmer and drier climate (<i>Abies alba</i>, <i>Larix decidua</i>, <i>Pinus sylvestris</i>) compared to the sites of tree origins. Monitored species exhibited remarkably distinct growth and water balance patterns over the 2015 growing season characterised with the highly above normal temperature and uneven precipitation distribution. <i>A. alba</i> exhibited smooth continuous growth least affected by varying environmental conditions. Of all analysed species, only <i>A. alba</i> showed significant positive correlations of radial growth and ΔW with temperature and global radiation, despite environmental water limitations. The lowest cumulative growth, lower negative values of ΔW and greater MDS of <i>L. decidua</i> indicate a higher water limitation of this species. The results showed more pronounced sensitivity of <i>P. sylvestris</i> to increased temperature and drought. All monitored variables of environmental conditions, except precipitation, significantly influenced MDS values of all studied tree species. Based on 30 variables describing radial stem growth patterns and water status we identified large inter-species variability and discrete species-specific groups, while the indicators of growth and water status of <i>L. decidua</i> and <i>P. sylvestris</i> were similar and the most different patterns were observed between <i>A. alba</i> and <i>L. decidua</i>. The behaviour of <i>P. abies</i> was closer to <i>A. alba</i> than to the other two species.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"109 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mosquito (Diptera: Culicidae) species diversity and abundance patterns across tree height and microclimatic gradients in Indiana, USA","authors":"Sajjad Khan, Madison G. Abel, Christopher S. Bibbs, Ary Faraji, Luis F. Chaves","doi":"10.1007/s11756-024-01698-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01698-2","url":null,"abstract":"<p>Climate, land use and land cover change influence infectious disease dynamics, particularly vector-borne diseases. Knowledge about mosquito ecology in southern Indiana is limited. Here, we present results from a season long study where we sampled mosquitoes at the Hickory Ridge Fire Tower in Hoosier National Forest, Monroe County, Indiana, USA. Using BG-Pro traps with BG-Lure and LED lights weekly from 23 May to 31 October 2023, we collected 178 mosquitoes over 24 weeks of sampling, encompassing a total of 120 trap-nights, with an average of 1.48 ± 3.41 (SD) mosquitoes per trap-night. The species accumulation curve for all the samples was flat, indicating a comprehensive sampling of species. We collected 10 species with a Chao2 ± SE species richness estimate of 14.46 ± 7.14. The dominant species was <i>Aedes vexans</i> (<i>n</i> = 58, 36%), followed by <i>Culex</i> spp. (composed of morphologically indistinguishable <i>Culex pipiens</i> and <i>Culex restuans</i>) (<i>n</i> = 51, 31%), <i>Culex erraticus</i> (<i>n</i> = 16, 10%), and <i>Aedes triseriatus</i> (<i>n</i> = 15, 9%). Because mosquitoes are ectothermic, they are highly sensitive to microclimatic variables such as temperature, humidity, and rainfall. Our time series analysis showed a significant association of mosquito abundance with the variability (SD and kurtosis) of the environmental variables we studied highlighting the importance of weather fluctuations in mosquito ecology. Our study highlights how weather variability shapes mosquito abundance, thus impacting disease vectors like <i>Culex</i> spp. and <i>Aedes triseriatus</i> with implications for arbovirus transmission in the context of climate change.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"61 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Artemisia annua (Asteraceae) leaf extract on immunity in Galleria mellonella (Lepidoptera: Pyralidae)","authors":"Rahile Öztürk, Serhat Kaya","doi":"10.1007/s11756-024-01677-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01677-7","url":null,"abstract":"<p>Sweet sagewort (<i>Artemisia annua</i>) is a species that has been used for many years to treat high fever due to its artemycin content. Artemycin is the active ingredient in antimalarial drugs. Additionally, the plant’s leaves are consumed as tea or aqueous extract. Invertebrate model organism <i>Galleria mellonella</i> is preferred in immune studies due to its ease of administration, natural immunity shared with mammals, including humans, and rapid determination of results. In this research, we systematically assessed the impact of <i>A. annua</i> extract on the immune system of animals by employing the <i>G. mellonella</i> model organism. We meticulously examined key facets of innate immune responses, including total hemocyte count, encapsulation-melanization, and phenoloxidase activity. Furthermore, we delved into the effects on antioxidant enzyme activity, specifically superoxide dismutase, catalase, and lipid peroxidase. The hemocyte count showed a statistically significant decrease across all given doses of the <i>A. annua</i> extract when compared to the control groups. The <i>A. annua</i> extract had no effect on the catalase activity, malondialdehyde amount, phenoloxidase activity, or melanization response of <i>G. mellonella</i> larvae, according to the findings of our study. Nonetheless, it enhanced SOD activity. In comparison to the control groups, it decreased the strong encapsulation response, which is a cell-mediated immune response, at all doses. While the <i>A. annua</i> extract had little effect on enzymatic processes in animals, it reduced cell-mediated immune responses by altering cell behaviours and count with cytotoxic and genotoxic effects.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"18 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140882162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of lunar phases and oceanographic parameters on green turtle nesting in Rocas Atoll","authors":"Débora Melo Mendonça, Luiz Drude de Lacerda, Karoline Fernanda Ferreira Agostinho, Luisa Diele-Viegas","doi":"10.1007/s11756-024-01695-5","DOIUrl":"https://doi.org/10.1007/s11756-024-01695-5","url":null,"abstract":"<p>Animals can synchronize their reproductive behavior patterns with biological rhythms and environmental conditions. Understanding these patterns is particularly critical for conserving endangered species like sea turtles. This study analyzed the relationship between the nesting behavior of the green turtle (<i>Chelonia myda</i>s) and lunar luminosity, lunar phases, and sea surface temperature (SST) in the Atol das Rocas Biological Reserve, Brazil. Field data were obtained through nightly monitoring during the breeding seasons from 2018 to 2020. Although lunar luminosity and lunar phases did not exhibit a statistically significant effect, the highest number of nesting occurrences was observed during the full moon and new moon, which may be related to the spring tide. The SST was the only variable showing a statistically significant relationship with complete oviposition, incomplete oviposition, and false crawl occurrences, indicating the preference of turtles to come ashore at higher temperatures (around 29.5 °C). Furthermore, while lunar luminosity and lunar phases may have some influence on the nesting pattern of the green turtle, they do not limit it, as turtles come ashore under different observed environmental conditions.\u0000</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"8 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140831034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of new taxa in Neotropical Desmiphorini (Coleoptera: Cerambycidae: Lamiinae)","authors":"Oscar Pérez-Flores, Víctor Hugo Toledo-Hernández","doi":"10.1007/s11756-024-01671-z","DOIUrl":"https://doi.org/10.1007/s11756-024-01671-z","url":null,"abstract":"<p>Two new genera and two species of Desmiphorini are described from Mexico: <i>Evrysoma cinereum</i> <b>gen. n.</b>, <b>sp. n.</b> from Querétaro and San Luis Potosí; <i>Kuklimia balam</i> <b>gen. n.</b>, <b>sp. n.</b> from Chiapas. Diagnostic characters of the new species are discussed and photographs of the type material are provided. A key to Mexican genera of Desmiphorini is provided.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"61 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140831491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and molecular responses of basil (Ocimum basilicum) to silver stress: a comparison between silver nanoparticles and silver nitrate treatments","authors":"Ali Zareei, Hossein Abbaspour, Maryam Peyvandi, Ahmad Majd","doi":"10.1007/s11756-024-01627-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01627-3","url":null,"abstract":"<p>With the extensive utilization of nanotechnology, silver nanoparticles (AgNPs) are prevalent nanomaterials that may entail ecological risks by their potential translocation into plant systems. The present study investigated the physiological and molecular responses of basil seedlings (<i>Ocimum basilicum</i>) subjected to AgNPs or silver nitrate (AgNO₃) for 7 days. The seedlings were treated with 0, 4, 10, or 40 mg/L of AgNO₃ or AgNPs in Hoagland’s solution. Both treatments resulted in significant accumulation of Ag in the roots and shoots, with higher levels in the roots of AgNO₃-treated seedlings. AgNPs increased plant biomass at 4 mg/L, while AgNO₃ decreased it at all concentrations. Both treatments reduced the total chlorophyll, carotenoids, and carbohydrates, with more pronounced effects in AgNO₃-treated seedlings. Both treatments also induced oxidative stress, as indicated by increased levels of H₂O₂, malondialdehyde (MDA), and proline, and enhanced activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). However, these responses were more evident in AgNO₃-treated seedlings, especially at higher concentrations. Quantitative real-time PCR analysis revealed that both treatments induced the upregulation of genes encoding oxidative stress tolerance-related enzymes, such as FSD1, MSD1, CSD1, CATa, CATb, APXa and APXb, in the basil seedling shoots. These results suggest that AgNPs are less toxic to basil plants than AgNO₃ and that basil plants can activate physiological and molecular mechanisms to cope with Ag-induced oxidative stress.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"25 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140831071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}