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Keratin hydrolysates: a sustainable product in biotechnology sectors by microbial conversion 角蛋白水解物:微生物转化生物技术领域的可持续产品
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-21 DOI: 10.1007/s11756-024-01725-2
Kanchan Mukesh, Priya Kannan, Lilly M. Saleena
{"title":"Keratin hydrolysates: a sustainable product in biotechnology sectors by microbial conversion","authors":"Kanchan Mukesh, Priya Kannan, Lilly M. Saleena","doi":"10.1007/s11756-024-01725-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01725-2","url":null,"abstract":"<p>The increasing population and demand for food and feed have increased the urge to find protein sources from waste products. Due to poor management of waste valorization, it has become a pollutant to the environment. This waste can be converted into a valuable product by microbial degradation. Feather waste from poultry farms can be efficiently processed into hydrolysates, serving as an additive or in its crude form for animal feed and detergents. This approach not only reduces pollution but also boosts the economy of a country. Keratin is a hard fibrous protein, insoluble in water and organic solvents. They are accumulated in nature and are major components of feathers, nails, hairs, and wool. Microorganisms like bacteria, fungi, and actinomycetes can degrade keratin by producing the keratinase enzyme. Keratinases are thought to be promising biocatalysts for the production of animal nutrients, protein supplements, leather processing, fibre modification, detergent formulations, and pharmaceutical, cosmetic, and biomedical industries. An overview of keratin structure and composition, the mechanism of microbial hydrolysis of keratin, and their possible uses in biotechnological sectors are presented in this review.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"31 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141496159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The net-tube caddisflies (Trichoptera: Psychomyioidea: Xiphocentronidae) from the Brazilian Amazon: discovery of new species and reports of new distributional records 巴西亚马逊河流域的网管蝶(毛翅目:精神鞘翅目:Xiphocentronidae):新物种的发现和新分布记录的报告
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-20 DOI: 10.1007/s11756-024-01700-x
Gleison R. Desidério, Albane Vilarino, Laissa S. Santos, Marcos A. R. Pires, Ana M. Pes, Talissa Matos, Karina Dias-Silva, Neusa Hamada
{"title":"The net-tube caddisflies (Trichoptera: Psychomyioidea: Xiphocentronidae) from the Brazilian Amazon: discovery of new species and reports of new distributional records","authors":"Gleison R. Desidério, Albane Vilarino, Laissa S. Santos, Marcos A. R. Pires, Ana M. Pes, Talissa Matos, Karina Dias-Silva, Neusa Hamada","doi":"10.1007/s11756-024-01700-x","DOIUrl":"https://doi.org/10.1007/s11756-024-01700-x","url":null,"abstract":"<p>Xiphocentronidae comprises eight genera found across tropical regions. In Brazil, two genera are present: <i>Xiphocentron</i> Brauer, 1870 and <i>Machairocentron</i> Schmid, 1982. <i>Xiphocentron</i> is further divided into five subgenera: <i>Xiphocentron</i> (<i>Antillotrichia</i>) Banks, 1941, <i>X</i>. (<i>Glyphocentron</i>) Schmid, 1982, <i>X</i>. (<i>Rhamphocentron</i>) Schmid, 1982<i>, X</i>. (<i>Sphagocentron</i>) Schmid, 1982<i>,</i> and <i>X</i>. (<i>Xiphocentron</i>). <i>Xiphocentron</i> (<i>Antillotrichia</i>) is the most species-rich subgenus and the only one occurring in South America. In Brazil, there are 18 species of <i>X</i>. (<i>Antillotrichia</i>), mainly distributed in the Atlantic Forest, with one species recorded in the Brazilian Amazon. <i>Machairocentron</i>, another Neotropical genus, comprises nine species, but only one species is known in Brazil. Recent research in the Brazilian Amazon led to the discovery of three new species of <i>Xiphocentron</i>: <i>Xiphocentron</i> (<i>Antillotrichia</i>) <i>moa</i> <b>sp. n.</b>, <i>Xiphocentron tapajos</i> <b>sp. n.</b>, and <i>Xiphocentron</i> (<i>Antillotrichia</i>) <i>xingu</i> <b>sp. n.</b> This study describes and illustrates these species, focusing on adult males, and highlighting the distinctive morphology of segment IX and inferior appendages. New distribution records are provided for <i>X</i>. (<i>Antillotrichia</i>) <i>sclerothrix</i> Pes &amp; Hamada, 2013 and <i>M</i>. <i>falciforme</i> Pes &amp; Hamada, 2013 . Additionally, <i>M</i>. <i>amahuaca</i> Vilarino, Salles &amp; Bispo, 2023 and <i>X</i>. (<i>Antillotrichia</i>) <i>surinamense</i> Flint, 1974 are reported for the first time in Brazil.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"77 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141500607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leaf temperatures of an Austrian oak are below photosynthetic temperature thresholds during a heatwave in Central Europe 中欧热浪期间奥地利橡树叶片温度低于光合作用温度阈值
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-17 DOI: 10.1007/s11756-024-01722-5
Norbert Kunert
{"title":"Leaf temperatures of an Austrian oak are below photosynthetic temperature thresholds during a heatwave in Central Europe","authors":"Norbert Kunert","doi":"10.1007/s11756-024-01722-5","DOIUrl":"https://doi.org/10.1007/s11756-024-01722-5","url":null,"abstract":"<p>The summer of 2022 has been the so far hottest summer on record in Central Europe. High temperatures negatively affect the physiology of plants and cause considerable thermal stress in particular on the leaf level. The assessment of the temperature-dependent decline of the quantum use efficiency of the photosystem II (Fv/Fm) has gained much popularity to quantify the leaf-level sensitivity to thermal stress. An open question is whether leaves heat to those in vitro estimated threshold temperatures on hot days or if plants can avoid heat stress through transpirational cooling. Therefore, leaf temperatures were monitored on a non-native Austrian oak (<i>Quercus cerris</i>) during a heatwave in July 2022 and compared to observed air temperature and leaf thermal traits assessed with a chlorophyll fluorometer. The highest air temperature recorded during the heatwave was 42.5 °C and surpassed the breaking point temperature (temperature at 5% decline of Fv/Fm; T5) by 0.3 °C, but was 6.1 °C lower than T50 (temperature at 50% decline of Fv/Fm). However, during the hottest day, the maximum leaf temperature was significantly below the air temperature. Even the directly illuminated leaf facing south reached a maximum temperature of only 38.7 °C but reached 39.8 °C on the second hottest day when the air temperature went up to 39.6 °C. All leaves showed a certain degree of homeothermy as the slope between leaf temperature and air temperature was 0.83 (P &lt; 0.05). In conclusion, Austrian oak can buffer thermal stress during heatwaves to a certain degree, however, leaf temperatures are only marginally below critical threshold temperatures.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"216 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
What is the diversity and pattern of network interactions parasite-host in amphibians (Anura) from Caatinga domain? – A meta-analysis 卡廷加域两栖动物(Anura)寄生-宿主网络互动的多样性和模式是什么?- 荟萃分析
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-17 DOI: 10.1007/s11756-024-01717-2
Ednalva da Silva Santos, Isabella Hevily Silva Torquato, Drausio Honorio Morais, Paulo Cascon, Charles de Sousa Silva
{"title":"What is the diversity and pattern of network interactions parasite-host in amphibians (Anura) from Caatinga domain? – A meta-analysis","authors":"Ednalva da Silva Santos, Isabella Hevily Silva Torquato, Drausio Honorio Morais, Paulo Cascon, Charles de Sousa Silva","doi":"10.1007/s11756-024-01717-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01717-2","url":null,"abstract":"<p>The first records in the literature compiled on the parasitic fauna of amphibians in Northeastern Brazil date back to the 1990s. Since then, several new studies have been published on parasite-host relationships, parasite communities, and descriptions of new taxa. However, only in the last decade has there been a significant increase in these studies. Given this growth, we aim to provide a complete and updated compilation of helminth records associated with amphibians from the Brazilian Northeastern region and to analyse the dynamics and network structure formed between parasites and their hosts. Therefore, 33 studies were found in the specialized literature that addressed data from eight families, 15 genera, and 34 species of anuran amphibians, distributed mainly in areas of the morphoclimatic domain of the Caatinga and Atlantic Forest remnants in the Brazilian Northeast. These data correspond to 35% of the total known species of the Caatinga, with Leptodactylidae being the most representative taxon. Regarding helminths, 51 species were recorded, belonging to 20 families and 32 genera. To evaluate the structure of the network, we used measures of connectivity, nestedness, modularity, and centrality, that were considered to identify key species. The web presented 247 interactions with a highly connected structure formed by two parasite generalist species, non-nested and non-modular. We concluded that anuran amphibians from the Brazilian Northeast possess a high parasitic diversity, being Bufonidae and Leptodactylidade taxa considered fundamental for the network structure. Herein, we provided the first analysis of the global framework of parasite communities in amphibians from Brazilian Northeast, by using antagonistic network interactions.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"123 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distribution of plant species Iris sibirica and its vegetation affinity in Slovakia 植物物种西伯利亚鸢尾的分布及其在斯洛伐克的植被亲缘关系
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-17 DOI: 10.1007/s11756-024-01719-0
Richard Hrivnák, Michal Slezák, Matej Dudáš, Dobromil Galvánek, Tereza Labovská, Tatiana Miháliková
{"title":"Distribution of plant species Iris sibirica and its vegetation affinity in Slovakia","authors":"Richard Hrivnák, Michal Slezák, Matej Dudáš, Dobromil Galvánek, Tereza Labovská, Tatiana Miháliková","doi":"10.1007/s11756-024-01719-0","DOIUrl":"https://doi.org/10.1007/s11756-024-01719-0","url":null,"abstract":"<p><i>Iris sibirica</i> is a threatened plant species of the Central European flora, but its distribution and vegetation ecology in Slovakia have not been studied so far. Therefore, we aimed to compile a complex chorology of this species and to analyse phytosociological plots with the species occurrence. Our results suggest that <i>Iris sibirica</i> occurs almost in the whole Slovakia, with two distributional centres situated in the south-western region (Pannonian bioregion) and in the southern regions of central Slovakia (Carpathian bioregion), but a higher proportion of recent records was identified in the Carpathians. Vegetation classification using the TWINSPAN algorithm divided 80 vegetation plots into 5 clusters. They were interpreted syntaxonomically as follows: i) <i>Calthion palustris</i> alliance, ii) <i>Deschampsion cespitosae</i> alliance, iii) <i>Molinion caeruleae</i> alliance, iv) vegetation of the successional stages and transitional status between wet meadows (<i>Calthion palustris</i> and <i>Molinion caeruleae</i>) and fens (<i>Scheuchzerio palustris-Caricetea fuscae</i>), and v) vegetation with a transitional status between hygrophilous, fen vegetation of the alliances <i>Molinion caeruleae</i>, <i>Caricion davallianae</i>, <i>Caricion fuscae</i> and mesic habitats. Variation in the species composition of vegetation plots, analysed using detrended correspondence analysis (DCA) and interpreted using Ellenberg indicator values for vascular plants, showed a shift along the first DCA axis from taxa typical for nutrient-rich and shaded but thermophilic sites to taxa of open and relatively cold habitats on nutrient-poor soils. The second DCA axis corresponded to the moisture gradient, which controlled the pattern of plant species richness.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"19 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141500606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First insights into the complete mitochondrial genome of a deep-sea eel Congriscus megastoma (Anguilliformes, Congroidei, Congridae): characterization and phylogenetic inference 首次发现深海鳗鲡Congriscus megastoma(鳗形目,鳗鲡科,Congridae)的完整线粒体基因组:特征描述和系统发育推断
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-17 DOI: 10.1007/s11756-024-01718-1
Yuping Liu, Peiyi Xiao, Tianyan Yang
{"title":"First insights into the complete mitochondrial genome of a deep-sea eel Congriscus megastoma (Anguilliformes, Congroidei, Congridae): characterization and phylogenetic inference","authors":"Yuping Liu, Peiyi Xiao, Tianyan Yang","doi":"10.1007/s11756-024-01718-1","DOIUrl":"https://doi.org/10.1007/s11756-024-01718-1","url":null,"abstract":"<p>The complete mitochondrial DNA sequence of a deep-sea eel <i>Congriscus megastoma</i> (Anguilliformes: Congridae) was determined by high throughput sequencing technology. As the first mitogenome of the genus <i>Congriscus</i> up to now, its entire mtDNA sequence was 17,616 bp in length, including 37 mitochondrial genes (13 protein-coding genes, 2 rRNA genes, 22 tRNA genes) and 2 control regions, which was inconsistent with that of the typical vertebrate mitogenomes. In addition, the genes of <i>C. megastoma</i> exhibited an obvious rearrangement. <i>ND6</i> and tRNA-Glu were transferred between tRNA-Thr and tRNA-Pro, as well as a doubled control region appeared. The tandem duplication-random loss (TDRL) model was an ideal explanation for this irregular gene reorganization phenomenon. The Bayesian phylogenetic tree showed that Congridae species were divided into four clades, suggesting their non-monophyletic origin. <i>Congriscus</i> had the closest relationships with <i>Thalassenchelys</i> and <i>Coloconger</i>. Species with gene rearrangement separately clustered as one clade. The results provided a basis for future studies of mitochondrial DNA structure and phylogeny of Anguilliformes.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"172 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of the dynamic approach for detection of Salmonella Typhi biofilm via optimizations of interaction with safranin dye 通过优化与黄樟素染料的相互作用,开发检测伤寒沙门氏菌生物膜的动态方法
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-04 DOI: 10.1007/s11756-024-01716-3
Aditya Upadhyay, Dharm Pal, Awanish Kumar
{"title":"Development of the dynamic approach for detection of Salmonella Typhi biofilm via optimizations of interaction with safranin dye","authors":"Aditya Upadhyay, Dharm Pal, Awanish Kumar","doi":"10.1007/s11756-024-01716-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01716-3","url":null,"abstract":"<p><i>Salmonella</i> Typhi infection is showing as a negative alarm for human health due to the occurrence of life-threatening typhoid fever and the emergence of antibiotic resistance. The infection becomes chronic due to the biofilm formation of <i>S.</i> Typhi, which plays a significant role in antibiotic resistance. The <i>S.</i> Typhi can survive under various stress conditions such as antibiotic stress, host immune stress, environmental stress, and nutritional stress in the biofilm stage. Biofilm provides a protective cover to the <i>S.</i> Typhi and prevents the entry of antibiotics inside the bacterial cells. Thus, biofilm is the root cause of the problem. Therefore, this study explores the easy detection of <i>S.</i> Typhi biofilms using safranin dye with specific concentrations. We performed the biofilm growth kinetics, qualitative assessment of the biofilm formations, and safranin interactions to finalize the specific concentrations of safranin (0.5 mg/mL) to detect the <i>S.</i> Typhi biofilm condition. The study was validated at 0.5 mg/mL safranin concentrations with the help of RSM analysis and kinetics study of the typhoidal biofilm. The study is important because the obtained results indicated the precise detection of <i>S.</i> Typhi biofilm, which is useful for all phases of biofilm detection. The study provided new directions for typhoidal biofilm staining that could be an emerging methodology in the area of health sciences.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"21 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141255335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia 斯洛伐克与伏甲 Xylosandrus germanus 相关的真菌
IF 1.5 4区 生物学
Biologia Pub Date : 2024-06-04 DOI: 10.1007/s11756-024-01712-7
Katarína Pastirčáková, Katarína Baková, Katarína Adamčíková, Marek Barta, Michal Lalík, Martin Pavlík, Andrej Kunca, Juraj Galko, Martin Pastirčák
{"title":"Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia","authors":"Katarína Pastirčáková, Katarína Baková, Katarína Adamčíková, Marek Barta, Michal Lalík, Martin Pavlík, Andrej Kunca, Juraj Galko, Martin Pastirčák","doi":"10.1007/s11756-024-01712-7","DOIUrl":"https://doi.org/10.1007/s11756-024-01712-7","url":null,"abstract":"<p>The black timber bark beetle (<i>Xylosandrus germanus</i>) is a strongly invasive ambrosia beetle and an important forest pest in Slovakia. This pest is closely associated with symbiotic fungi used as its food source. We investigated the fungi associated with <i>X. germanus</i> adults in Slovakia. In this study, <i>Beauveria bassiana</i>, <i>B. pseudobassiana</i>, <i>Clonostachys rosea</i>, <i>Fusarium oxysporum</i>, <i>Ophiostoma quercus</i>, <i>Phaeoacremonium scolyti</i>, and <i>Talaromyces amestolkiae</i> were isolated and identified by morphological and molecular analyses. The fungus <i>Ophiostoma quercus</i> was most frequently isolated from living beetles, while the entomopathogenic <i>Beauveria bassiana</i> was the most commonly isolated from dead beetles. The morphological descriptions of fungi based on isolates from the surface of <i>X. germanus</i> adults are provided.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"19 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141256797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production of N-glycosylated alcohol dehydrogenase in Escherichia coli 在大肠杆菌中生产 N-糖基化醇脱氢酶
IF 1.5 4区 生物学
Biologia Pub Date : 2024-05-31 DOI: 10.1007/s11756-024-01707-4
Zdenko Levarski, Stanislava Bírová, Kristina Hriňová, Johana Dlapová, Eva Struhárňanská, Lenka Levarská, Ján Turňa, Stanislav Stuchlík
{"title":"Production of N-glycosylated alcohol dehydrogenase in Escherichia coli","authors":"Zdenko Levarski, Stanislava Bírová, Kristina Hriňová, Johana Dlapová, Eva Struhárňanská, Lenka Levarská, Ján Turňa, Stanislav Stuchlík","doi":"10.1007/s11756-024-01707-4","DOIUrl":"https://doi.org/10.1007/s11756-024-01707-4","url":null,"abstract":"<p>N-glycosylation of recombinant proteins using bacterial glycosylation system has proven to be a valuable although developing tool ultimately applicable to various industries. When used for enzyme engineering, it offers the possibility of increased stability or immobilization route and thus increasing effectiveness of e.g. biotransformation or other biocatalysis procedures. One such promising enzyme is alcohol dehydrogenase (ADH) for use in redox biotransformation reactions. Given the current possibilities of recombinant enzyme production, including major advances in glycoengineering and glycoprotein production in bacterial organisms, the aim of this work was the production of thermotolerant ADH from <i>Rhodococcus ruber</i> (RrADH) in glycosylated form in <i>Escherichia coli</i>. We have successfully developed a dual plasmid expression system enabling glycosylation of target proteins utilizing a glyco-tag approach. We were able to produce RrADH in soluble form and at the same time we detected a bacterial glycan conjugated to RrADH as well as the activity of the enzyme. The glycan bound to recombinant enzyme can be used for oriented covalent immobilization of the enzyme, which would increase the potential for its practical application in biotransformation of various compounds.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"59 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In silico analysis of a heme-thiolate peroxidase gene discovered in an ectomycorrhizal fungus of Carpathian primeval forest: implications for biotechnological applications 对喀尔巴阡山原始森林一种外生菌根真菌中发现的血红硫酸盐过氧化物酶基因的硅学分析:对生物技术应用的影响
IF 1.5 4区 生物学
Biologia Pub Date : 2024-05-30 DOI: 10.1007/s11756-024-01709-2
Bohuš Kubala, Peter Ferianc, Katarína Chovanová, Marcel Zámocký
{"title":"In silico analysis of a heme-thiolate peroxidase gene discovered in an ectomycorrhizal fungus of Carpathian primeval forest: implications for biotechnological applications","authors":"Bohuš Kubala, Peter Ferianc, Katarína Chovanová, Marcel Zámocký","doi":"10.1007/s11756-024-01709-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01709-2","url":null,"abstract":"<p> In this work, we focus on the identification of novel fungal peroxygenase gene belonging to the peroxidase-peroxygenase superfamily. We applied a metagenomic approach on soil samples from primeval forest and appropriate bioinformatics tools for analysis of obtained genomic DNA sequence. Peroxidases are ubiquitous metalloenzymes that are able to reduce reactive peroxides, mainly hydrogen peroxide, into water, whereas several substrates can be concomitantly oxidized during their catalytic reaction. Our purpose was to collect unique peroxygenase sequence data originating from a preserved biotope for a robust phylogenetic reconstruction of a particular gene family coding for highly versatile heme-thiolate peroxidases that has peculiar yet undiscovered representatives among ectomycorrhizal fungi. We identified unique DNA sequence, 812 bp long, from ectomycorrhizal <i>Suillus</i> species coding for a heme-thiolate peroxidase with 1 typical intron that appears distinctive for Carpathian forests. After translation in corresponding protein sequence 251 amino acids long we could identify typical signatures of this peroxygenase. On the proximal side of heme we found the conserved P-C-P triad responsible for efficient ligation of heme iron thus influencing the reactivity of this peroxidase. On the distal side we recognized the E-H-D-X-S-L motif for interaction with a stabilizing magnesium ion. Maximum likelihood reconstruction of protein phylogeny revealed with a high bootstrap support the presence of a monophyletic HTP4 clade originating in numerous <i>Suillus</i> representatives. Together with sister clades of edible <i>Boletus</i> and poisonous <i>Paxillus</i> containing diverse peroxygenases these newly discovered heme catalyst can be considered for application of oxyfunctionalization of organic molecules.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"54 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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