BiologiaPub Date : 2024-07-11DOI: 10.1007/s11756-024-01729-y
Daniel Bravo-Pérez, Cecilia Hernández-Zepeda, Cristobal Chaidez-Quiroz, Daysi de la Caridad Pérez-Brito, Jean-Pierre González-Gómez, Yereni Minero-García, Gabriela Rosiles-González, Víctor Hugo Carrillo-Jovel, Oscar Alberto Moreno-Valenzuela
{"title":"Composition of the whiteflies microbiome in populations with and without insecticide applications in Yucatan Mexico","authors":"Daniel Bravo-Pérez, Cecilia Hernández-Zepeda, Cristobal Chaidez-Quiroz, Daysi de la Caridad Pérez-Brito, Jean-Pierre González-Gómez, Yereni Minero-García, Gabriela Rosiles-González, Víctor Hugo Carrillo-Jovel, Oscar Alberto Moreno-Valenzuela","doi":"10.1007/s11756-024-01729-y","DOIUrl":"https://doi.org/10.1007/s11756-024-01729-y","url":null,"abstract":"<p>The whitefly <i>Bemisia tabaci</i> (Gennadius) has coevolved with intracellular endosymbiotic bacteria that help supplement their nutrient-poor diets with essential amino acids and carotenoids; however the type of host species and the agricultural practices can influence the composition of the endosymbiotic bacterial community. In this study, the main goal was to explore the diversity of endosymbiont communities in whitefly populations growing in peppers (<i>Capsicum chinense</i> Jacq. under greenhouse and <i>Capsicum annuum</i> L. backyard) exposed to chemical insecticides (treatment MB1) and non-exposed (treatment MB2). The whiteflies were identified through phylogenetic analysis of the mitochondrial Cytochrome Oxidase I gene sequences. Bacterial endosymbionts were characterized by high-throughput sequencing of the V3 region of the 16S rRNA gene, which amplifies a fragment of around 180 bp. Sequence analysis yielded in MB1 eight Phylum, nine classes, 30 orders, 35 families, 64 genera, and 20 species, while in MB2 it was obtained eight Phylum, 11 classes, 26 orders, 37 families, 61 genera, and 18 species. The most abundant phylum in the two samples analyzed was Proteobacteria followed by Chlamydiae and Bacteroidetes. The phyla that presented the greatest taxonomic diversity of bacteria at the genus level in both samples were Proteobacteria, Actinobacteria and Firmicutes. According to the evaluated alpha diversity indices, low richness and diversity were obtained in the two samples, but when comparing the two samples, MB2 showed the greatest richness and diversity with 1,698 with the Shannon index.. The results obtained are a first approximation to describe the impact of insecticides used on peppers on the diversity and richness of whitefly endosymbiotic bacteria.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"25 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biogenic synthesis and characterization of gold nanoparticles using transformed mesophilic Escherichia coli BL21 and thermophilic Thermus thermophilus HB27","authors":"Mariana Erasmus, Oladayo Amed Idris, Adegoke Isiaka Adetunji, Errol Duncan Cason","doi":"10.1007/s11756-024-01733-2","DOIUrl":"https://doi.org/10.1007/s11756-024-01733-2","url":null,"abstract":"<p>Gold nanoparticles have numerous applications, many of which are notable in industries. The biosynthesis of gold nanoparticles offers an easy, effective, green, and eco-friendly approach. In organisms capable of synthesizing nanoparticles, enzymes and proteins are responsible for the structural and functional modifications that lead to their formation. These include ABC transporter, peptide-binding proteins, which are dependent on abiotic parameters. This study uses the purified ABC transporter, peptide-binding protein transformed from <i>Thermus scotoductus</i> SA-01 and expressed in mesophilic <i>Escherichia coli</i> BL21 and thermophilic <i>Thermus thermophilus</i> HB27 hosts for the biosynthesis of gold nanoparticles at different concentrations, temperatures, and pH values. Gold nanoparticle formation was evaluated with a range of gold (III) concentrations (0–10 mM), incubated at temperatures ranging from 30–85 ºC and pH levels from 3.6–9.0. Transmission electron microscopy (TEM), energy dispersive X-ray spectrometry (EDX), and UV–Vis absorption spectroscopy were used to characterise the formation of nanoparticles. In all of the protein reactions, UV–Vis absorbance peaks at approximately 520–560 nm confirmed the formation of gold nanoparticles. Optimum nanoparticle synthesis was observed at pH values ranging from 5.5 to 9.0, gold (III) solution (HAuCl<sub>4</sub>) concentrations from 0.5–2.0 mM, and a maximum temperature of 65ºC in the mesophilic host and 85ºC in the thermophilic host, indicating the significance of temperature in both hosts for the expression and bioactivity of the purified ABC transporter protein. However, the biogenic formation of gold nanoparticles using <i>E. coli</i> and <i>T. thermophilus</i> hosts was not monodispersed, suggesting a necessity for further development of the procedure.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"44 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141586987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiologiaPub Date : 2024-07-08DOI: 10.1007/s11756-024-01724-3
Mykyta Peregrym, Snežana Dragićević
{"title":"The current knowledge about the distribution of Portulaca species (Portulacaceae) within the Balkan Peninsula","authors":"Mykyta Peregrym, Snežana Dragićević","doi":"10.1007/s11756-024-01724-3","DOIUrl":"https://doi.org/10.1007/s11756-024-01724-3","url":null,"abstract":"<p>This study addresses the complex taxonomy and distribution of the genus <i>Portulaca</i> L. (<i>Portulacaceae</i>) within the Balkans, focusing on the often-debated species composition and distribution patterns in the <i>P.</i> <i>oleracea</i> aggregate. Our research aims to resolve this ambiguity by addressing two key questions: 1) Which <i>Portulaca</i> taxa are present in the Balkans, and 2) What is the current distribution of these taxa here? To achieve this, we conducted literature reviews, seed collection, species identification using micromorphological characteristics, and distribution analysis. Our findings reveal the presence of four distinct species in the Balkans: <i>P. oleracea</i> agg., <i>P. grandiflora</i>, <i>P. pilosa</i> and <i>P. umbraticola</i>. Additionally, within the <i>P.</i> <i>oleracea</i> complex, twelve microspecies have been identified, primarily differentiated based on seed micromorphology. Besides, five species (<i>P. daninii</i>, <i>P. granulato-stellulata</i>, <i>P. rausii</i>, <i>P. sardoa</i>, and <i>P. trituberculata</i>) have been documented in Montenegro, and one species (<i>P.</i> <i>nitida</i>) – in Slovenia for the first time. This study provides a valuable contribution to the botanical knowledge of the Balkan Peninsula, offering the recommendations for future studies of the <i>P.</i> <i>oleracea</i> complex for the solution of the taxonomic puzzle in this aggregate.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"268 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiologiaPub Date : 2024-07-08DOI: 10.1007/s11756-024-01699-1
Victor M. Ghirotto, Edgar B. Crispino, Phillip W. Engelking, Pedro Alvaro Barbosa Aguiar Neves, Pablo Valero, Thiago Ferreira Soares, Thiago Silva-Soares, Thiago José De Souza Alves, Pedro Sisnando, Raphael A. Heleodoro, Oskar Conle, Frank Hennemann, Pedro I. Chiquetto-Machado
{"title":"Thirteen new species of Urucumania (Phasmatodea: Pseudophasmatidae) stick insects from Brazil and Bolivia","authors":"Victor M. Ghirotto, Edgar B. Crispino, Phillip W. Engelking, Pedro Alvaro Barbosa Aguiar Neves, Pablo Valero, Thiago Ferreira Soares, Thiago Silva-Soares, Thiago José De Souza Alves, Pedro Sisnando, Raphael A. Heleodoro, Oskar Conle, Frank Hennemann, Pedro I. Chiquetto-Machado","doi":"10.1007/s11756-024-01699-1","DOIUrl":"https://doi.org/10.1007/s11756-024-01699-1","url":null,"abstract":"<p>Stick insects (Phasmatodea) in general are remarkable for presenting striking camouflage-related adaptations. Even so, a considerable number of species are colorful and present other defense mechanisms, such as the New World lineage Pseudophasmatidae. Within this family, <i>Urucumania</i> currently comprises two described species occurring in Brazil, Bolivia and Paraguay, both of which have small scale-like wings. <i>Urucumania</i> is currently placed in Anisomorphini. However, two similar species under <i>Pseudophasma</i> present remarkable similarities with <i>Urucumania</i> species. These two species, <i>Pseudophasma nigrovittatum</i> and <i>Pseudophasma dentata</i>, are the only <i>Pseudophasma</i> presenting scale-like wings. Both inhabit Brazil, are known from a single sex and present a generally similar coloration pattern contrasting to that of <i>Urucumania</i>. Aiming to resolve whether these and other similar specimens represent distinct species, and if they belong to <i>Urucumania</i> rather than to <i>Pseudophasma</i>, we conducted a careful analysis of the external morphology of both sexes, eggs and the male genitalia. We transfer both scale-like winged <i>Pseudophasma</i> to <i>Urucumania</i>, redescribing them based on both sexes showing that the two described species are in fact distinct, and furthermore present 13 new species of <i>Urucumania</i> from Brazil and Bolivia: <i>Urucumania pirulai</i> <b>sp. n.</b>, <i>U. varellai</i> <b>sp. n.</b>, <i>U. guadanuccii</i> <b>sp. n.</b>, <i>U. atilai</i> <b>sp. n.</b>, <i>U. intervalica</i> <b>sp. n.</b>, <i>U. brasil</i> <b>sp. n.</b>, <i>U. tapirape</i> <b>sp. n.</b>, <i>U. rasocatarinensis</i> <b>sp. n.</b>, <i>U. dilatata</i> <b>sp. n.</b>, <i>U. sertaneja</i> <b>sp. n.</b>, <i>U. albopunctata</i> <b>sp. n.</b>, <i>U. oriomadeirensis</i> <b>sp. n.</b> and <i>U. candanga</i> <b>sp. n.</b></p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"10 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Description of a new vagrant Aceria species (Acari: Eriophyidae) on Artemisia scoparia Waldst. & Kit. (Asteraceae) in northwest Iran and a key to the world species associated with Artemisia spp.","authors":"Arash Honarmand, Parisa Lotfollahi, Zahra Hashemi–Khabir, Syamak Hanifeh, Xiao–Feng Xue","doi":"10.1007/s11756-024-01721-6","DOIUrl":"https://doi.org/10.1007/s11756-024-01721-6","url":null,"abstract":"<p>To fill the gap of eriophyoid mites in Iran, a field survey was carried out in northwest Iran (Aras dam lake, West Azerbaijan Province), in summer 2022. As a result, a new vagrant <i>Aceria</i> species (Acari: Eriophyoidea: Eriophyidae) associated with <i>Artemisia scoparia</i> Waldst. & Kit. (Asteraceae) was discovered. The new species is illustrated and described herein as <i>Aceria artemiparia</i> <b>sp. n.</b> No apparent damage was observed on the host. This is the second report of an eriophyoid mite species associated to <i>A. scoparia</i> host plant. <i>Aceria artemiparia</i> <b>sp. n.</b> is unique among 24 <i>Aceria</i> spp. associated with <i>Artemisia</i> species by having a smooth prodorsal shield and 8–rayed empodium. Furthermore, we provided a checklist and morphological key of eriophyoid mites associated with <i>Artemisia</i> spp. of the world.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"30 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiologiaPub Date : 2024-07-08DOI: 10.1007/s11756-024-01723-4
César A. Díaz-Marín, Aurelio Ramírez-Bautista, Hibraim A. Pérez-Mendoza, Aaron García-Rosales, Christopher K. Akcali
{"title":"Thermal divergence in a color polymorphic lizard inhabiting contrasting environments in central Mexico","authors":"César A. Díaz-Marín, Aurelio Ramírez-Bautista, Hibraim A. Pérez-Mendoza, Aaron García-Rosales, Christopher K. Akcali","doi":"10.1007/s11756-024-01723-4","DOIUrl":"https://doi.org/10.1007/s11756-024-01723-4","url":null,"abstract":"<p>The niche partitioning hypothesis predicts differential use of resources among morphs depending on resource dynamics and morph frequencies. Additionally, morphs might select microhabitats favorable for the regulation of body temperature through behavioral mechanisms. Intra- and intersexual social interactions among morphs can also affect patterns of niche partitioning. Here, we evaluated whether body size, thermal traits (body and environmental temperatures, and behavioral thermoregulation), microhabitat use, and perch height differ among male and female throat color morphs of the lizard <i>Sceloporus grammicus</i> in four different habitat types. Color morphs of both sexes differed in body size, thermal traits, and perch height but not microhabitat use. Lizards of grassland and xeric scrubland were larger, and had greater values of thermal traits but exhibited lower perch height than lizards of mountain cloud forest and pine-oak forest. We found clear evidence of thermal niche partitioning and partial space niche partitioning among color morphs of both sexes of <i>S. grammicus</i> in central Mexico. These results improve our knowledge of the ecology of color polymorphic vertebrate species and show that color morphs of each sex have specific trait combinations to facilitate the use of thermal resources.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"38 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiologiaPub Date : 2024-07-04DOI: 10.1007/s11756-024-01714-5
Shokoofeh Hajihashemi, Omolbanin Jahantigh
{"title":"Acclimation to salinity stress through maintaining the redox status by H2O2 and arginine application in Vicia faba","authors":"Shokoofeh Hajihashemi, Omolbanin Jahantigh","doi":"10.1007/s11756-024-01714-5","DOIUrl":"https://doi.org/10.1007/s11756-024-01714-5","url":null,"abstract":"<p>The generation of reactive oxygen/nitrogen species under either natural or stressful conditions activates antioxidant systems to balance the redox status in plant cells. The effect of seed priming with low concentrations of H<sub>2</sub>O<sub>2</sub> and/or arginine (Arg) was examined on redox status of salinity-stressed <i>Vicia faba</i> in the present study. Salinity stress produced H<sub>2</sub>O<sub>2</sub> and caused oxidative damage to photosynthetic pigments and membrane lipids represented by reduction in chlorophyll contents and carbohydrate production, and high accumulation of malondialdehyde, denoted as oxidative distress. Under saline conditions, Arg and/or H<sub>2</sub>O<sub>2</sub> priming increased the activity of antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase), non-enzymatic antioxidants (phenols, flavonoids, anthocyanins and carotenoids), proline and total antioxidant activity (based on the FRAP method) followed by a reduction in the malondialdehyde content and an increase in the chlorophylls and water soluble carbohydrates contents. Altogether, the seed priming with H<sub>2</sub>O<sub>2</sub> and Arg could constitute a ‘priming memory’ in seeds of <i>V. faba</i>, which recruited upon a subsequent salinity stress-exposure and induced stress-tolerance of primed beans trough invoking antioxidant systems.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"49 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141552152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiologiaPub Date : 2024-07-03DOI: 10.1007/s11756-024-01730-5
Rajan Preenanka, Muhammed Puratheparampilchira Safeena
{"title":"In vitro lytic potential of Streptococcus bacteriophage against multidrug resistant Streptococcus agalactiae","authors":"Rajan Preenanka, Muhammed Puratheparampilchira Safeena","doi":"10.1007/s11756-024-01730-5","DOIUrl":"https://doi.org/10.1007/s11756-024-01730-5","url":null,"abstract":"<p><i>Streptococcus agalactiae</i> stands out as a significant bacterial pathogen, causing substantial economic losses in the aquaculture sector. Given the challenges posed by multidrug resistance, this study explores the potential of phage therapy as an alternative to antibiotics in biocontrol strategies. The focus is on evaluating the multidrug resistance profile of <i>S. agalactiae</i> isolated from a tilapia aquaculture farm, with particular attention to the strain KSA/01, which exhibits resistance to seven structurally different classes of antibiotics and a notable MAR index of around 0.6. In response to this challenge, the study successfully identifies and isolates the specific and lytic phage SAP-13 targeting the multidrug-resistant strain KSA/01. Transmission electron microscopy reveals that SAP-13 shares morphological characteristics with the <i>Siphoviridae</i> family. In a one-step growth curve, the phage demonstrates a substantial burst size of approximately 610 PFU/cell over a short burst period and the phage exhibited stability across various physicochemical parameters such as temperature, pH, and salinity. In vitro lytic ability of SAP-13 at different multiplicity of infection underscores its potential to effectively eliminate <i>S. agalactiae</i>, particularly at an MOI of 0.01. Consequently, these findings suggest that phage SAP-13 exhibits high infectivity against <i>S. agalactiae</i>, presenting a promising avenue for addressing multidrug-resistant strains in aquaculture.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"13 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141514881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antimicrobial peptides: a novel and natural approach as antibiofouling mediator","authors":"Harshita Sharma, Vivek Dave, Ekta Tyagi, Anand Prakash","doi":"10.1007/s11756-024-01703-8","DOIUrl":"https://doi.org/10.1007/s11756-024-01703-8","url":null,"abstract":"<p>Antimicrobial peptides (AMPs) are natural ribosomal synthesized small peptides and are crucial effectors in all living organisms. AMPs are diverse polypeptides with varying amino acid composition and structure that often exhibit broad-spectrum and fast-acting antibacterial properties. As a result, AMPs offer a great deal of promise for restorative application in medicine, agriculture, and the environment. The maritime, industrial, and healthcare sectors all suffer major losses as a result of biofouling. This article will review antimicrobial peptides (AMPs), their structure and major class of AMPs that are reported as anti-biofouling agents, evolutionary concept of AMP, mechanism of action of AMPs against biofilm formation, intracellular active AMPs, synthetic AMPs, promising AMP modifications reported and AMP databases to understand AMPs specificity and activity for rational designing.</p><p>Therefore, this review will provide a comprehensive assessment of AMPs numerous functions as well as to detect their therapeutic potential and avoid industry losses incurred by biofilms.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"34 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141500608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiologiaPub Date : 2024-06-26DOI: 10.1007/s11756-024-01728-z
Song Gao, Xianghui Qi, Ran Lu, Song Zhu, Guoshi Kan, Tatiana Stanishneva-Konovalova, Yingfeng An, Fuping Lu
{"title":"Production of fructo-oligosaccharide syrup through catalysis of Bacillus amyloliquefaciens levanase and immobilized Ba-SacB on cell lysate","authors":"Song Gao, Xianghui Qi, Ran Lu, Song Zhu, Guoshi Kan, Tatiana Stanishneva-Konovalova, Yingfeng An, Fuping Lu","doi":"10.1007/s11756-024-01728-z","DOIUrl":"https://doi.org/10.1007/s11756-024-01728-z","url":null,"abstract":"<p>Levan is a type of fructan with β-(2, 6) fructosyl linkage. In recent years, levan has been used in the medicinal, food and chemical industries for its excellent physicochemical properties of good thermal stability, low viscosity, pseudoplasticity, and non-swelling in water. The levansucrase is key enzyme responsible for biosynthesis of levan from substrate sucrose. In the present study cell lysate of <i>Escherichia coli</i> was used as support for convenient immobilization of Ba-SacB (i.e., Ba-SacB-Cell-Lysate) under the action of dopamine. Compared with the free Ba-SacB, the Ba-SacB-Cell-Lysate showed surprisingly enhanced pH stability in the pH range 6–9, and improved relative activities in the pH ranges 4–5 and 7–10. With the presence of 10% (v/v) butyl alcohol, 5mmol/L Fe<sup>3+</sup>, and after vortex for 90 min, the Ba-SacB-Cell-Lysate showed up to 14.32 times, 4.63 times, and 9.2 times higher relative activities than the free Ba-SacB. The Ba-SacB-Cell-Lysate kept nearly 81% of the residual activity after 9 cycles of reuse. The fructo-oligosaccharide syrup (FOS) was efficiently produced through the catalysis of levanase with the substrate of levan produced by Ba-SacB-Cell-Lysate, and the preparation conditions were optimized by Box-Behnken response surface methodology.</p>","PeriodicalId":8978,"journal":{"name":"Biologia","volume":"64 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141496161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}