Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-07 DOI:10.1007/s11756-024-01683-9

Khadidja Meziane, Bruno Guinand, Mohamed Hichem Kara, Lamya Chaoui

引用次数: 0

Abstract

Thin-lipped mullet (Chelon ramada) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.

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开发薄唇鲻鱼（Chelon ramada）（Risso，1827 年）的微卫星标记资源

薄唇鲻（Chelon ramada）是地中海的重要资源，也是水产养殖的候选物种。由于微卫星位点仍然是评估育种计划或种群评估的首选标记，我们在此提供并分享该物种目前缺乏的 3883 个物种特异性微卫星位点资源。该资源包括用于聚合酶链式反应（PCR）扩增的引物对。在相同的聚合酶链反应条件下，对包括二核苷酸、三核苷酸和四核苷酸位点在内的九个具有正确扩增成功率的位点子集进行了专门测试，为研究开辟了新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.