开发薄唇鲻鱼（Chelon ramada）（Risso，1827 年）的微卫星标记资源

IF 1.4 4区生物学 Q3 BIOLOGY

Biologia Pub Date : 2024-05-07 DOI:10.1007/s11756-024-01683-9

Khadidja Meziane, Bruno Guinand, Mohamed Hichem Kara, Lamya Chaoui

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引用次数: 0

摘要

薄唇鲻（Chelon ramada）是地中海的重要资源，也是水产养殖的候选物种。由于微卫星位点仍然是评估育种计划或种群评估的首选标记，我们在此提供并分享该物种目前缺乏的 3883 个物种特异性微卫星位点资源。该资源包括用于聚合酶链式反应（PCR）扩增的引物对。在相同的聚合酶链反应条件下，对包括二核苷酸、三核苷酸和四核苷酸位点在内的九个具有正确扩增成功率的位点子集进行了专门测试，为研究开辟了新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)

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Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)

Thin-lipped mullet (Chelon ramada) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.

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来源期刊

Biologia 生物-生物学

CiteScore

3.30

自引率

6.70%

发文量

290

审稿时长

6 months

期刊介绍： Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.