Journal of glycomics & lipidomics最新文献_第9页

Lectin Array Analysis of Purified Lipooligosaccharide: A Method for the Determination of Molecular Mimicry 纯化低脂多糖的凝集素阵列分析:一种分子仿真性测定方法

Journal of glycomics & lipidomics Pub Date : 2012-01-01 DOI: 10.4172/2153-0637.1000103

E. A. Semchenko, M. Moutin, V. Korolik, J. Tiralongo, C. Day

{"title":"Lectin Array Analysis of Purified Lipooligosaccharide: A Method for the Determination of Molecular Mimicry","authors":"E. A. Semchenko, M. Moutin, V. Korolik, J. Tiralongo, C. Day","doi":"10.4172/2153-0637.1000103","DOIUrl":"https://doi.org/10.4172/2153-0637.1000103","url":null,"abstract":"Surface glycosylation of bacteria is involved in many critical host-microbe interactions. Lectin arrays consisting of diverse carbohydrate binding proteins have proven to be an important tool for evaluating a wide variety of glycosylation, including that present on whole bacteria. However, assessing glycosylation on whole bacteria using lectin arrays may not reflect bacterial glycosylation, but interactions between bacteria and the glycosylation present on lectins. The lipooligosaccharide of Campylobacter jejuni NCTC 11168 and 81-176 are known to mimic the human monosialylated gangliosides. This molecular mimicry by C. jejuni can result in the post infection sequelae Guillain–Barre syndrome. Using C. jejuni as a model system and a discreet lectin and antibody array, a method, applicable to many organisms has been developed and validated by to screening of the purified lipooligosaccharide of C. jejuni for molecular mimicry to monosialylated gangliodises. In case of C. jejuni, knowing whether clinically important bacterial strains are capable of inducing severe autoimmune responses may aid in prevention and/or early diagnosis of debilitating post infection conditions.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"2 1","pages":"1000105-1-1000105-5"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70229800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Remarkable Indications-How the Glycomics is Coded in Cells and Tissues 显著的适应症-糖组学如何在细胞和组织中编码

Journal of glycomics & lipidomics Pub Date : 2012-01-01 DOI: 10.4172/2153-0637.1000E107

Jozef Nahalka

引用次数: 0

Establishment of an Analytical Method for Sialyl Glycoprotein Extraction from the Experimental Hormone-Induced Estrous Cycle of the MouseOvary 实验性激素诱导小鼠卵巢发情周期中唾液糖蛋白提取分析方法的建立

Journal of glycomics & lipidomics Pub Date : 2012-01-01 DOI: 10.4172/2153-0637.S4-001

M. Yokoyama, Yasuhiro Takegawa, T. Yamashita

{"title":"Establishment of an Analytical Method for Sialyl Glycoprotein Extraction from the Experimental Hormone-Induced Estrous Cycle of the MouseOvary","authors":"M. Yokoyama, Yasuhiro Takegawa, T. Yamashita","doi":"10.4172/2153-0637.S4-001","DOIUrl":"https://doi.org/10.4172/2153-0637.S4-001","url":null,"abstract":"Ovaries are often found in pairs as part of the vertebrate female reproductive system that maintains immature egg cells until they become mature with ovulation. The ovary is also an internal secretion organ for both estrogen and progesterone. Estrogen is responsible for the appearance of secondary sex characteristics of females at puberty and for the maturation and maintenance of the reproductive organs. Progesterone functions mainly to regulate the condition of the endometrium, preparing it to accept a fertilized egg. On the other hand, glycans are a main component of the animal cell surface and show structural changes during early development and cell differentiation. In the present study, to clarify the relation between the changes in glycan structures and ovarian functions such as ovulation, we used a system in which ovulation was induced by sex hormone treatment. As a result, it was found that glycoproteins including terminal sialic acids, especially N-glycolyl neuraminic acid-type glycans, increased during development of the ovary. Furthermore, to find specific glycoproteins with the N-glycolyl neuraminic acid-type glycan that were induced by hormone treatment, we established a method for the purification of glycoproteins, using sialic acid followed by detection using mass spectrometry. Applying the modified protocol, transferrin could be detected as the protein that changed the amount of glycans in the developmental cycle of the ovary. Thus, this protocol will be a useful tool for the detection of glycoproteins with sialic acid residues. It may also be a valuable method for use with other organs.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"2012 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2153-0637.S4-001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70234539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protein Lipidation and Its Analysis 蛋白质脂化及其分析

Journal of glycomics & lipidomics Pub Date : 2012-01-01 DOI: 10.4172/2153-0637.S2-001

Gemma Triola

引用次数: 10

Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF -Special Reference to Human Brain Ganglioside Molecular Species TLC-Blot与MALDI-TOF结合的复杂脂质分子种成像技术——人脑神经节苷类分子种专用参考

Journal of glycomics & lipidomics Pub Date : 2012-01-01 DOI: 10.4172/2153-0637.S2-004

T. Valdes-Gonzalez, Naoko Goto-Inoue, T. Hayasaka, Hironobu Ishiyama, M. Setou, T. Taki

{"title":"Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF -Special Reference to Human Brain Ganglioside Molecular Species","authors":"T. Valdes-Gonzalez, Naoko Goto-Inoue, T. Hayasaka, Hironobu Ishiyama, M. Setou, T. Taki","doi":"10.4172/2153-0637.S2-004","DOIUrl":"https://doi.org/10.4172/2153-0637.S2-004","url":null,"abstract":"Glycosphingolipids and phospholipids from white matter of human hippocampus, were analyzed by a sequential procedure of two-dimensional TLC (2d-TLC), transference of separated lipids to a PVDF membrane by a TLC-Blot equipment and Mass spectrometry (MS) analysis with an ion-trap type MALDI-TOF equipment. The method is simple and quick; very small amount of sample (0.1 mg of brain tissue) is enough to analyze all lipid components. The 2d-TLC provided excellent separation and the MS analyses allowed identifying the characteristic profile of molecular species for individual glycolipids and phospholipids. The results of MS analyses on gangliosides showed that di- and tri-sialogangliosides are richer in d20:1 sphingosine-containing ceramide than monosialogangliosides, suggesting the presence of sialylation selection after GM1 gangliosides. Then we analyzed ganglioside molecular species obtained from different brain regions by using MS imaging technology. The MS images of individual gangliosides provided clear visual profiles in terms of molecular species distribution. The imaging profiles were region dependent and also indicated that the sialyltransferase toward GM1 ganglioside prefers to select d20:1 sphingosine containing molecule. This technology provides visual characterization of individual phospholipid and glycosphingolipid molecular species and informs us about the metabolic characterization of target tissue, opening a new gate for colorful lipidomics research.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"18 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70234385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Clustered Conserved Cysteines in Hyaluronan Synthase Mediate Cooperative Activation by Mg²⁺ Ions and Severe Inhibitory Effects of Divalent Cations. 透明质酸合酶中聚集性保守半胱氨酸介导Mg2+离子的协同激活和二价阳离子的严重抑制作用。

Journal of glycomics & lipidomics Pub Date : 2011-11-15 DOI: 10.4172/2153-0637.S1-001

Valarie L Tlapak-Simmons, Andria P Medina, Bruce A Baggenstoss, Long Nguyen, Christina A Baron, Paul H Weigel

{"title":"Clustered Conserved Cysteines in Hyaluronan Synthase Mediate Cooperative Activation by Mg2+ Ions and Severe Inhibitory Effects of Divalent Cations.","authors":"Valarie L Tlapak-Simmons, Andria P Medina, Bruce A Baggenstoss, Long Nguyen, Christina A Baron, Paul H Weigel","doi":"10.4172/2153-0637.S1-001","DOIUrl":"10.4172/2153-0637.S1-001","url":null,"abstract":"Hyaluronan synthase (HAS) uses UDP-GlcUA and UDP-GlcNAc to make hyaluronan (HA). Streptococcus equisimilis HAS (SeHAS) contains four conserved cysteines clustered near the membrane, and requires phospholipids and Mg2+ for activity. Activity of membrane-bound or purified enzyme displayed a sigmoidal saturation profile for Mg2+ with a Hill coefficient of 2. To assess if Cys residues are important for cooperativity we examined the Mg2+ dependence of mutants with various combinations of Cys-to-Ala mutations. All Cys-mutants lost the cooperative response to Mg2+. In the presence of Mg2+, other divalent cations inhibited SeHAS with different potencies (Cu2+~Zn2+ >Co2+ >Ni2+ >Mn2+ >Ba2+ Sr2+ Ca2+). Some divalent metal ions likely inhibit by displacement of Mg2+-UDP-Sugar complexes (e.g. Ca2+, Sr2+ and Ba2+ had apparent Ki values of 2-5 mM). In contrast, Zn2+ and Cu2+ inhibited more potently (apparent Ki ≤ 0.2 mM). Inhibition of Cys-null SeHAS by Cu2+, but not Zn2+, was greatly attenuated compared to wildtype. Double and triple Cys-mutants showed differing sensitivities to Zn2+ or Cu2+. Wildtype SeHAS allowed to make HA prior to exposure to Zn2+ or Cu2+ was protected from inhibition, indicating that access of metal ions to sensitive functional groups was hindered in processively acting HA•HAS complexes. We conclude that clustered Cys residues mediate cooperative interactions with Mg2+ and that transition metal ions inhibit SeHAS very potently by interacting with one or more of these -SH groups.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"Suppl 1 ","pages":"001"},"PeriodicalIF":0.0,"publicationDate":"2011-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4176928/pdf/nihms-341993.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32705756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidomics - A Novel Strategy for Probing into Systems Biology Operations: Networking between Glycome, Lipidome, Proteome, and Genome 脂质组学-探索系统生物学操作的新策略:糖蛋白、脂质组、蛋白质组和基因组之间的网络

Journal of glycomics & lipidomics Pub Date : 2011-04-15 DOI: 10.4172/2153-0637.1000E101

S. Kotha, Rishi B. Patel, T. Elton, M. H. Davidson, Narasimham L. Parin

{"title":"Lipidomics - A Novel Strategy for Probing into Systems Biology Operations: Networking between Glycome, Lipidome, Proteome, and Genome","authors":"S. Kotha, Rishi B. Patel, T. Elton, M. H. Davidson, Narasimham L. Parin","doi":"10.4172/2153-0637.1000E101","DOIUrl":"https://doi.org/10.4172/2153-0637.1000E101","url":null,"abstract":"One of the fascinating and challenging marvels of biological systems is the dynamic cellular compartmentalization aided by the structure and function of diverse and complex molecules, mainly carbohydrates, lipids, proteins, and nucleic acids which constitute the glycome, lipidome, proteome, and genome, respectively. Nevertheless, these molecular entities are in a constant flux adapting to and modulating the functions of the biological system at the membrane, organelle, cellular, and organ levels and finally regulating the organismal structure and function(s). Remarkably, the microsystems (microcosms) including glycome, lipidome, proteome, and genome operate by interwoven networks and communicate with each other through the complex and dynamic molecular signal transduction mechanisms (Figure 1) involving enzymes, hormones, receptors, lipids, carbohydrates, nucleic acids, and bioactive molecules, to name a few [1,2]. Thus, the entire biological system (macrocosm) at the organismal level is constantly regulated (homeostasis) by the well-controlled interconnected operations of the microsystems.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"01 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2011-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70233075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Study on the Preparation and Increasing Production Mechanism of a Novel Environmentally Friendly Cotton Seed Coating Agent 新型环保型棉种包衣剂的制备及增产机理研究

Journal of glycomics & lipidomics Pub Date : 2011-03-04 DOI: 10.4172/2153-0637.1000102

D. Zeng, Wu Shan, Qinggang Xiao

引用次数: 6

Changes in N-acylethanolamine Pathway Related Metabolites in a Rat Model of Cerebral Ischemia/Reperfusion. 大鼠脑缺血/再灌注模型中n -酰基乙醇胺通路相关代谢物的变化

Journal of glycomics & lipidomics Pub Date : 2011-01-01

Aruna Kilaru, Pamela Tamura, Puja Garg, Giorgis Isaac, David Baxter, R Scott Duncan, Ruth Welti, Peter Koulen, Kent D Chapman, Barney J Venables

{"title":"Changes in N-acylethanolamine Pathway Related Metabolites in a Rat Model of Cerebral Ischemia/Reperfusion.","authors":"Aruna Kilaru, Pamela Tamura, Puja Garg, Giorgis Isaac, David Baxter, R Scott Duncan, Ruth Welti, Peter Koulen, Kent D Chapman, Barney J Venables","doi":"","DOIUrl":"","url":null,"abstract":"In mammals, the endocannabinoid signaling pathway provides protective cellular responses to ischemia. Previous work demonstrated increases in long-chain N-acylethanolamines (NAE) in ischemia and suggested a protective role for NAE. Here, a targeted lipidomics approach was used to study comprehensive changes in the molecular composition and quantity of NAE metabolites in a rat model of controlled brain ischemia. Changes of NAE, its precursors, N-acylphosphatidylethanolamines (NAPE), major and minor phospholipids, and free fatty acids (FFA) were quantified in response to ischemia. The effect of intraperitoneal injection of N-palmitoylethanolamine (NAE 16:0) prior to ischemia on NAE metabolite and phospholipid profiles was measured. While ischemia, in general, resulted in elevated levels of N-acyl 16:0 and18:0 NAE, NAPE, and FFA species, pretreatment with NAE 16:0 reduced infarct volume, neurological behavioral deficits in rats, and FFA content in ischemic tissues. Pretreatment with NAE 16:0 did not affect the profiles of other NAE metabolites. These studies demonstrate the utility of a targeted lipidomics approach to measure complex and concomitant metabolic changes in response to ischemia. They suggest that the neuroprotective effects of exogenous NAE 16:0 and the reduction in inflammatory damage may be mediated by factors other than gross changes in brain NAE levels, such as modulation of transcriptional responses.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457465/pdf/nihms690267.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33367439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in N-acylethanolamine Pathway Related Metabolites in a Rat Model of Cerebral Ischemia/Reperfusion. 大鼠脑缺血/再灌注模型中n -酰基乙醇胺通路相关代谢物的变化

Journal of glycomics & lipidomics Pub Date : 2011-01-01 DOI: 10.4172/2153-0637.1000101

Aruna Kilaru, P. Tamura, P. Garg, G. Isaac, D. Baxter, R. Duncan, R. Welti, P. Koulen, K. Chapman, B. Venables

{"title":"Changes in N-acylethanolamine Pathway Related Metabolites in a Rat Model of Cerebral Ischemia/Reperfusion.","authors":"Aruna Kilaru, P. Tamura, P. Garg, G. Isaac, D. Baxter, R. Duncan, R. Welti, P. Koulen, K. Chapman, B. Venables","doi":"10.4172/2153-0637.1000101","DOIUrl":"https://doi.org/10.4172/2153-0637.1000101","url":null,"abstract":"In mammals, the endocannabinoid signaling pathway provides protective cellular responses to ischemia. Previous work demonstrated increases in long-chain N-acylethanolamines (NAE) in ischemia and suggested a protective role for NAE. Here, a targeted lipidomics approach was used to study comprehensive changes in the molecular composition and quantity of NAE metabolites in a rat model of controlled brain ischemia. Changes of NAE, its precursors, N-acylphosphatidylethanolamines (NAPE), major and minor phospholipids, and free fatty acids (FFA) were quantified in response to ischemia. The effect of intraperitoneal injection of N-palmitoylethanolamine (NAE 16:0) prior to ischemia on NAE metabolite and phospholipid profiles was measured. While ischemia, in general, resulted in elevated levels of N-acyl 16:0 and18:0 NAE, NAPE, and FFA species, pretreatment with NAE 16:0 reduced infarct volume, neurological behavioral deficits in rats, and FFA content in ischemic tissues. Pretreatment with NAE 16:0 did not affect the profiles of other NAE metabolites. These studies demonstrate the utility of a targeted lipidomics approach to measure complex and concomitant metabolic changes in response to ischemia. They suggest that the neuroprotective effects of exogenous NAE 16:0 and the reduction in inflammatory damage may be mediated by factors other than gross changes in brain NAE levels, such as modulation of transcriptional responses.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"1 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70229646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15