Lectin Array Analysis of Purified Lipooligosaccharide: A Method for the Determination of Molecular Mimicry

E. A. Semchenko, M. Moutin, V. Korolik, J. Tiralongo, C. Day
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引用次数: 9

Abstract

Surface glycosylation of bacteria is involved in many critical host-microbe interactions. Lectin arrays consisting of diverse carbohydrate binding proteins have proven to be an important tool for evaluating a wide variety of glycosylation, including that present on whole bacteria. However, assessing glycosylation on whole bacteria using lectin arrays may not reflect bacterial glycosylation, but interactions between bacteria and the glycosylation present on lectins. The lipooligosaccharide of Campylobacter jejuni NCTC 11168 and 81-176 are known to mimic the human monosialylated gangliosides. This molecular mimicry by C. jejuni can result in the post infection sequelae Guillain–Barre syndrome. Using C. jejuni as a model system and a discreet lectin and antibody array, a method, applicable to many organisms has been developed and validated by to screening of the purified lipooligosaccharide of C. jejuni for molecular mimicry to monosialylated gangliodises. In case of C. jejuni, knowing whether clinically important bacterial strains are capable of inducing severe autoimmune responses may aid in prevention and/or early diagnosis of debilitating post infection conditions.
纯化低脂多糖的凝集素阵列分析:一种分子仿真性测定方法
细菌的表面糖基化参与了许多关键的宿主-微生物相互作用。由多种碳水化合物结合蛋白组成的凝集素阵列已被证明是评估各种糖基化的重要工具,包括存在于整个细菌上的糖基化。然而,使用凝集素阵列评估整个细菌的糖基化可能不能反映细菌的糖基化,而是反映细菌与凝集素上存在的糖基化之间的相互作用。已知空肠弯曲杆菌NCTC 11168和81-176的低脂糖可以模拟人类单唾液化神经节苷。空肠梭菌的这种分子模仿可导致感染后后遗症格林-巴利综合征。以空肠梭菌为模型系统,采用离散的凝集素和抗体阵列,建立了一种适用于多种生物的方法,并通过筛选纯化的空肠梭菌低脂糖进行单唾液化神经节苷的分子模拟。在空肠梭菌的情况下,了解临床重要的细菌菌株是否能够诱导严重的自身免疫反应,可能有助于预防和/或早期诊断虚弱的感染后状况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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