Journal of glycomics & lipidomics最新文献_第3页

Lipidomics of Plasma High-Density Lipoprotein: Insights into Anti-Atherogenic Function 血浆高密度脂蛋白的脂质组学:抗动脉粥样硬化功能的见解

Journal of glycomics & lipidomics Pub Date : 2015-08-19 DOI: 10.4172/2153-0637.1000133

A. Kontush, M. Lhomme

{"title":"Lipidomics of Plasma High-Density Lipoprotein: Insights into Anti-Atherogenic Function","authors":"A. Kontush, M. Lhomme","doi":"10.4172/2153-0637.1000133","DOIUrl":"https://doi.org/10.4172/2153-0637.1000133","url":null,"abstract":"Low concentrations of high-density lipoprotein-cholesterol (HDL-C) represent a strong, independent risk factor for cardiovascular (CV) disease and atherosclerosis. The association between HDL-C and CV risk is thought to reflect multiple atheroprotective properties of HDL particles. The multiple biological functions of HDL particles are directly related to the presence of key bioactive lipid and protein components. Modern LC/MS/MS lipidomic approaches can be particularly useful to provide insights into molecular determinants of atheroprotective function of HDL. First comprehensive lipidomic studies of HDL particles in healthy subjects and in patients with CV disease or CV risk factors performed using modern lipidomic approaches have provided initial insights into lipid species profiles of human plasma HDL in health and disease. Such structure-function analyses of HDL bear the potential to identify clinically relevant, atheroprotective HDL components, which can contribute to the development of HDL-based therapies specifically designed to target beneficial subspecies of circulating HDL pool. Furthermore, HDL lipidomics can help identify novel biomarkers of HDL function, which may prove useful as biomarkers of cardiovascular risk superior to HDL-C levels.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"5 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2015-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2153-0637.1000133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Glycoproteomic Analysis of Human Hepatoblastoma Cell Lines Using Glycopeptide Capture and Mass Spectrometry 用糖肽捕获和质谱法分析人肝母细胞瘤细胞系的糖蛋白组学

Journal of glycomics & lipidomics Pub Date : 2015-07-15 DOI: 10.4172/2153-0637.1000132

Qinzhe Wang, Fei Zhao, Xiuxuan Sun, Shisheng Sun, Hanjie Yu, Y. Zhong, Yuan Hu, Zhuo Chen, Peixin Zhang, Zheng Li

引用次数: 2

Deciphering Hidden DNA Meta-Codes -The Great Unification & MasterCode of Biology 破译隐藏的DNA元密码——生物学的大统一与主密码

Journal of glycomics & lipidomics Pub Date : 2015-06-28 DOI: 10.4172/2153-0637.1000131

Jean-claude Perez

{"title":"Deciphering Hidden DNA Meta-Codes -The Great Unification & MasterCode of Biology","authors":"Jean-claude Perez","doi":"10.4172/2153-0637.1000131","DOIUrl":"https://doi.org/10.4172/2153-0637.1000131","url":null,"abstract":"This article presents multi-layered basic research culminating in what we call the Master Code of Biology. First, we review the “Formula for Life” discovery. This simple formula unifies all the components of life, structuring them in a kind of “Periodic table of Biology”. These components include bio-atoms, CONHSP, nucleotides, UTCAG, amino acids, DNA and RNA strands, proteins, genes, chromosomes and genomes. This discovery opens the door to powerful insights in exobiology, proposing a specific life-emerging constraint on the tuning and balancing of isotope proportions. Second, we introduce the “Master Code of Biology”, digital language supplying a common alphabet to the three fundamental languages of Genetics, Biology and Genomics. This synthesis goes above and beyond their three representative DNA, RNA and amino acids codes. There is a universal common code which unifies, connects and contains all these three languages. This “Master Code of Biology” provides a great Unification between the Master Code patterned images of Genomics (DNA) and Proteomics (amino acids), which appear highly correlated while RNA images curiously appear flat like a neutral or zero-like code. Third, the functionality of this discovery is evidenced by two examples. Analyzing textures of the Genomic and Proteomic patterned curves reveals the emergence of binary codes and discrete waveforms. These predict the well-known karyotypes- white/grey/black bands overlapping and characterizing the human chromosomes. Mapping “the Master Code of Biology” to SNP genomic areas, we show that SNPs are determined to be more functional by their location within the genome than by their local values– TCAG nucleotide changes. Finally, we demonstrate that a chromosomal DNA sequences systematic reshaping combined with periodic waves highlighted above brings out, at whole chromosome scale, interferometry-like interference fields manifested by resonances, tunings, and even resonances that exhibit Fibonacci number proportions differentiating human and great primates at whole chromosome4 scale.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"5 1","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2015-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Free Amino Acids Glycine and Glutamic Acid Inhibit Angiogenesis Induced by AGE in Bovine Retinal Endothelial Cells 游离氨基酸甘氨酸和谷氨酸抑制AGE诱导的牛视网膜内皮细胞血管生成

Journal of glycomics & lipidomics Pub Date : 2015-04-28 DOI: 10.4172/2153-0637.1000130

B. Devi, K. Coral, K. Sulochana, N. Angayarkanni

{"title":"Free Amino Acids Glycine and Glutamic Acid Inhibit Angiogenesis Induced by AGE in Bovine Retinal Endothelial Cells","authors":"B. Devi, K. Coral, K. Sulochana, N. Angayarkanni","doi":"10.4172/2153-0637.1000130","DOIUrl":"https://doi.org/10.4172/2153-0637.1000130","url":null,"abstract":"Previous studies from our lab have shown that amino acids act as antiglycating agents and can be beneficial in diabetes mellitus. Accumulation of advanced glycation end products (AGE) in uncontrolled diabetes mellitus can induce microvascular complications such as diabetic retinopathy that results in neovascularization in the retina. This study explored the effect of amino acids Glycine and Glutamic acid ( 0.5–2.5 mM) in mitigating the AGE (100 μg/ml) induced angiogenic effects in primary bovine retinal endothelial cells (BREC) cultured in vitro. Tube formation induced by AGE in the BREC cells were reduced by glycine and glutamic acid (p=0.05, p=0.008). Transwell migration assay revealed signficant inhibition of migration by glycine. Expression of actin cytoskelatal filaments that promotes migration was reduced predominantly by glycine than glutamic acid as seen by immuofluorescence. Leukocyte adhesion promoted by AGE treatment was reduced significantly by glycine (p=0.03) and glutamic acid (p=0.02). The mechanism was delineated in terms of AGE-RAGE/VEGF axis. The receptor for AGE (RAGE) and the VEGF expression was found to be decreased both at protein level and at mRNA level by glycine and glutamic acid treatment. mRNA expression of RAGE by qPCR revealed a maximal decrease of 60% by glycine at 2.5 mM but by glutamic acid at 0.5 mM and VEGF mRNA level showed maximal inhibition by glutamic acid at 2.5 mM. Western blot analysis showed that VEGF expression was predominantly reduced by glycine. This is the A¯Â¬Ârst study showing the anti angiogenic potential of the amino acids in an in vitro model of primary bovine retinal endothelial cell implying therapeutic potential in the management of diabetic retinopathy.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"5 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2153-0637.1000130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Protein Folding and Misfolding: A Perspective from Theory 蛋白质折叠与错误折叠:理论视角

Journal of glycomics & lipidomics Pub Date : 2015-02-27 DOI: 10.4172/2153-0637.1000128

A. Naeem, T. Khan, N. A. Fazili

引用次数: 2

Protein Glycation: A Firm Link to Cause Metabolic Disease and their Complications 蛋白糖基化:与代谢性疾病及其并发症密切相关

Journal of glycomics & lipidomics Pub Date : 2015-01-28 DOI: 10.4172/2153-0637.1000127

Saheem Ahmad, Z. Siddiqui

引用次数: 20

Lipidomics in Health and Diseases - Beyond the Analysis of Lipids 健康和疾病中的脂质组学——超越脂质分析

Journal of glycomics & lipidomics Pub Date : 2015-01-01 DOI: 10.4172/2153-0637.1000126

Mingming Li, Pengcheng Fan, Yu Wang

{"title":"Lipidomics in Health and Diseases - Beyond the Analysis of Lipids","authors":"Mingming Li, Pengcheng Fan, Yu Wang","doi":"10.4172/2153-0637.1000126","DOIUrl":"https://doi.org/10.4172/2153-0637.1000126","url":null,"abstract":"The role of lipids in human health and disease is taking the center stage. In the last decades, there has been an intense effort to develop suitable methodologies to discover, identify, and quantitatively monitor lipids in biological systems. Recent advancement of mass spectrometry technology has provided a variety of tools for global study of the lipid “Omes”, including the quantification of known lipid molecular species and the identification of novel lipids that possess pathophysiological functions. Lipidomics has thus emerged as a discipline for comprehensively illuminating lipids, lipidderived mediators and lipid networks in body fluids, tissues and cells. However, owing to the complexity and diversity of the lipidome, lipid research is challenging. Here, the experimental strategies for lipid isolation and characterization will be presented, especially for those who are new to the field of lipid research. Because lipids are known to participate in a host of protein signaling and trafficking pathways, the review emphasizes the understanding of interactions between cellular components, in particular the lipid-protein interrelationships. Novel tools for probing lipid-protein interactions by advanced mass spectrometric techniques will be discussed. It is expected that by integrating the approaches of lipidomics, transcriptomics and proteomics, a clear understanding of the complex functions of lipids will eventually be translated into human diseases.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"5 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2153-0637.1000126","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Comparison of Follicle-Stimulating Hormone Glycosylation Microheterogenity by Quantitative Negative Mode Nano-Electrospray Mass Spectrometry of Peptide-N Glycanase-Released Oligosaccharides. 肽- n聚糖酶释放寡糖定量负模式纳米电喷雾质谱法比较促卵泡激素糖基化微异质性。

Journal of glycomics & lipidomics Pub Date : 2015-01-01 DOI: 10.4172/2153-0637.1000129

George R Bousfield, Vladimir Y Butnev, William K White, Aaron Smalter Hall, David J Harvey

{"title":"Comparison of Follicle-Stimulating Hormone Glycosylation Microheterogenity by Quantitative Negative Mode Nano-Electrospray Mass Spectrometry of Peptide-N Glycanase-Released Oligosaccharides.","authors":"George R Bousfield, Vladimir Y Butnev, William K White, Aaron Smalter Hall, David J Harvey","doi":"10.4172/2153-0637.1000129","DOIUrl":"https://doi.org/10.4172/2153-0637.1000129","url":null,"abstract":"Glycans from six highly purified hFSH preparations were released by peptide-N-glycanase digestion and analyzed by negative mode nano-ESI mass spectrometry before and after neuraminidase digestion. Pituitary glycan structures were mainly high-mannose, di-, tri-, and tetra-antennary, and their abundance largely paralleled that reported by other investigators using different approaches. For most of the FSH preparations, the differences in glycosylation appeared to be restricted to relative abundances of the major glycan families, as defined by their neutral core oligosaccharide structures. Qualitative differences between glycan populations were largely relegated to those species that were lowest in abundance. Significant qualitative differences were noted in two cases. Recombinant GH3-hFSH triantennary glycans appeared to have the third antenna exclusively on the mannose6-branch, in contrast to all pituitary and urinary hFSH triantennary glycans, in which this antenna was exclusively attached to the mannose3-branch. The hypo-glycosylated hFSH preparation isolated from purified hLH was decorated with high mannose glycans that accounted for over 40% of the total in this population. As this preparation was found to be consistently 20-fold more active than hFSH24 in FSH receptor-binding assays, it appears that both macroheterogeneity and microheterogeneity in FSH preparations need to be taken into account.","PeriodicalId":89585,"journal":{"name":"Journal of glycomics & lipidomics","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2153-0637.1000129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33290934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Combining TLC Separation with MS Detection - A Revival of TLC 薄层色谱分离与质谱检测相结合——薄层色谱的复兴

Journal of glycomics & lipidomics Pub Date : 2015-01-01 DOI: 10.4172/2153-0637.1000E125

Katerina Matheis, B. Fuchs, Katharina Lemmnitzer, R. Süss, Hans Griesinger, S. Minarik, M. Oberle, M. Schulz, J. Schiller

引用次数: 8

HemeOxygenase-1: Transducer of Sterol Dys-Regulation in Alzheimer Disease 血红素加氧酶-1:阿尔茨海默病中固醇失调调节的传感器

Journal of glycomics & lipidomics Pub Date : 2014-10-20 DOI: 10.4172/2153-0637.1000124

J. Hascalovici, Schippers Hm

引用次数: 3