Bioelectromagnetics最新文献_第7页

Changes in Gene Expression After Exposing Arabidopsis thaliana Plants to Nanosecond High Amplitude Electromagnetic Field Pulses 拟南芥暴露于纳秒级高幅电磁场脉冲后的基因表达变化。

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-07-06 DOI: 10.1002/bem.22475

Alexis Porcher, Nancy Wilmot, Pierre Bonnet, Vincent Procaccio, Alain Vian

{"title":"Changes in Gene Expression After Exposing Arabidopsis thaliana Plants to Nanosecond High Amplitude Electromagnetic Field Pulses","authors":"Alexis Porcher, Nancy Wilmot, Pierre Bonnet, Vincent Procaccio, Alain Vian","doi":"10.1002/bem.22475","DOIUrl":"10.1002/bem.22475","url":null,"abstract":"The biological effects of exposure to electromagnetic fields due to wireless technologies and connected devices are a subject of particular research interest. Ultrashort high-amplitude electromagnetic field pulses delivered to biological samples using immersed electrodes in a dedicated cuvette have widely demonstrated their effectiveness in triggering several cell responses including increased cytosolic calcium concentration and reactive oxygen species (ROS) production. In contrast, the effects of these pulses are poorly documented when electromagnetic pulses are delivered through an antenna. Here we exposed Arabidopsis thaliana plants to 30,000 pulses (237 kV m−1, 280 ps rise-time, duration of 500 ps) emitted through a Koshelev antenna and monitored the consequences of electromagnetic fields exposure on the expression levels of several key genes involved in calcium metabolism, signal transduction, ROS, and energy status. We found that this treatment was mostly unable to trigger significant changes in the messenger RNA accumulation of calmodulin, Zinc-Finger protein ZAT12, NADPH oxidase/respiratory burst oxidase homolog (RBOH) isoforms D and F, Catalase (CAT2), glutamate-cystein ligase (GSH1), glutathione synthetase (GSH2), Sucrose non-fermenting-related Kinase 1 (SnRK1) and Target of rapamycin (TOR). In contrast, Ascorbate peroxidases APX-1 and APX-6 were significantly induced 3 h after the exposure. These results suggest that this treatment, although quite strong in amplitude, is mostly ineffective in inducing biological effects at the transcriptional level when delivered by an antenna. © 2023 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 1","pages":"4-15"},"PeriodicalIF":1.9,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22475","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10114420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information - Page 问题信息-页面

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-06-27 DOI: 10.1002/bem.22477

引用次数: 0

Pulsed Electromagnetic Fields Improved Peripheral Nerve Regeneration After Delayed Repair of One Month 脉冲电磁场对延迟修复1个月后周围神经再生的促进作用。

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-06-05 DOI: 10.1002/bem.22443

Zhu Keyan, Zhang Liqian, Xu Xinzhong, Jing Juehua, Xu Chungui

{"title":"Pulsed Electromagnetic Fields Improved Peripheral Nerve Regeneration After Delayed Repair of One Month","authors":"Zhu Keyan, Zhang Liqian, Xu Xinzhong, Jing Juehua, Xu Chungui","doi":"10.1002/bem.22443","DOIUrl":"10.1002/bem.22443","url":null,"abstract":"The goal of this study was to determine if postoperative pulsed electromagnetic fields (PEMFs) could improve the neuromuscular rehabilitation after delayed repair of peripheral nerve injuries. Thirty‐six Sprague–Dawley rats were randomly divided into sham group, control group, and PEMFs group. The sciatic nerves were transected except for the control group. One month later, the nerve ends of the former two groups were reconnected. PEMFs group of rats was subjected to PEMFs thereafter. Control group and sham group received no treatment. Four and 8 weeks later, morphological and functional changes were measured. Four and eight weeks postoperatively, compared to sham group, the sciatic functional indices (SFIs) of PEMFs group were higher. More axons regenerated distally in PEMFs group. The fiber diameters of PEMFs group were larger. However, the axon diameters and myelin thicknesses were not different between these two groups. The brain‐derived neurotrophic factor and vascular endothelial growth factor expressions were higher in PEMFs group after 8 weeks. Semi‐quantitative IOD analysis for the intensity of positive staining indicated that there were more BDNF, VEGF, and NF200 in PEMFs group. It's concluded that PEMFs have effect on the axonal regeneration after delayed nerve repair of one month. The upregulated expressions of BDNF and VEGF may play roles in this process. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 7-8","pages":"133-143"},"PeriodicalIF":1.9,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9579404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in the Optical Response of MSU and CPP Crystals During Magnetic Orientation: Possibility of Diagnosing Gout and Pseudogout 磁取向过程中MSU和CPP晶体光响应的差异:诊断痛风和伪痛风的可能性。

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-05-08 DOI: 10.1002/bem.22444

Yuka Takeuchi, Ryotaro Yoshikawa, Yoshifuru Mitsui, Masakazu Iwasaka, Mizushi Matsuda, Atom Hamasaki

{"title":"Differences in the Optical Response of MSU and CPP Crystals During Magnetic Orientation: Possibility of Diagnosing Gout and Pseudogout","authors":"Yuka Takeuchi, Ryotaro Yoshikawa, Yoshifuru Mitsui, Masakazu Iwasaka, Mizushi Matsuda, Atom Hamasaki","doi":"10.1002/bem.22444","DOIUrl":"10.1002/bem.22444","url":null,"abstract":"Pseudogout is crystalline arthritis. It has a similar clinical picture to that of gout, and it is difficult to distinguish the two diseases using conventional analysis methods. However, it is important to identify the different crystals responsible for these two cases because the treatment strategies are different. In a previous study, we reported magnetic orientation of monosodium urate (MSU) crystals, which are the causative agent of gout, at the permanent magnet level. In this study, we investigated the effect of an applied magnetic field on calcium pyrophosphate (CPP) crystals, which are the causative agent of pseudogout, and the difference in the magnetic responses of CPP and MSU crystals. We found that the CPP crystals were oriented in a magnetic field on milli-Tesla order because of the anisotropy of the diamagnetic susceptibility. In addition, the CPP crystals exhibited different anisotropic magnetic properties from those of MSU crystals, which led to a characteristic difference between the orientations of the two crystals. That is, we found that the causative agents of gout and pseudogout responded differently to a magnetic field. This report suggests that the discrimination between CPP and MSU by optical measurements is possible by application of magnetic fields appropriately. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 7-8","pages":"204-210"},"PeriodicalIF":1.9,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9432397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Meaningful Attempt: Applying Dielectric Barrier Discharge Plasma to Induce Polarization of Macrophages 介质阻挡放电等离子体诱导巨噬细胞极化的一次有意义的尝试

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-04-27 DOI: 10.1002/bem.22446

Haoran Yu, Wendan Cheng, Chengbiao Ding, Ziyu Li, Wenchong Ouyang, Qi Liu, Zhengwei Wu, Juehua Jing

{"title":"A Meaningful Attempt: Applying Dielectric Barrier Discharge Plasma to Induce Polarization of Macrophages","authors":"Haoran Yu, Wendan Cheng, Chengbiao Ding, Ziyu Li, Wenchong Ouyang, Qi Liu, Zhengwei Wu, Juehua Jing","doi":"10.1002/bem.22446","DOIUrl":"10.1002/bem.22446","url":null,"abstract":"Macrophage polarization plays an important role in many macrophage-related diseases. This study was designed to preliminarily explore the effects of dielectric barrier discharge (DBD) plasma on the polarization direction and cell activity of macrophages with different phenotypes (ie, M0, M1, and M2). The M1 macrophage marker inducible nitric oxide synthase (iNOS) and M2 macrophage marker cluster of differentiation 206 (CD206) were detected by western blot (WB). The effects of DBD plasma on macrophage viability were analyzed by using a cell counting kit-8 detection kit. M0, M1, and M2 macrophages exhibited a decrease in iNOS expression and an increase in CD206 expression after the DBD plasma intervention. Additionally, the decrease in macrophage viability remained non-significant after initiating the intervention. DBD plasma can promote the transformation of M0 and M1 macrophages to M2 macrophages, and can further enhance the expression of the M2 macrophage phenotype marker CD206. Our study not only demonstrates the potential therapeutic value of DBD plasma for macrophage-related diseases, but it also provides a new direction for research to improve the treatment of macrophage-related diseases. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 5-6","pages":"107-118"},"PeriodicalIF":1.9,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9685395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information - Page 发行信息‐页面

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-04-24 DOI: 10.1002/bem.22448

引用次数: 0

Evaluation of the Effects of Power-Frequency Magnetic Field Exposure on B-Cell Differentiation From Human Hematopoietic Stem/Progenitor Cells 工频磁场暴露对人造血干细胞/祖细胞b细胞分化影响的评价

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-04-18 DOI: 10.1002/bem.22447

Masayuki Takahashi, Naoko Furuya

{"title":"Evaluation of the Effects of Power-Frequency Magnetic Field Exposure on B-Cell Differentiation From Human Hematopoietic Stem/Progenitor Cells","authors":"Masayuki Takahashi, Naoko Furuya","doi":"10.1002/bem.22447","DOIUrl":"10.1002/bem.22447","url":null,"abstract":"The causal relationship between exposure to power‐frequency magnetic fields (MFs) and childhood leukemia has long been controversial. The most common type of childhood leukemia is acute B‐lymphoblastic leukemia caused by abnormal proliferation of B cells in the early differentiation process. Here, we focused on B‐cell early differentiation and aimed to evaluate the effects of exposing cells to power‐frequency MF. First, we optimized an in vitro differentiation protocol of human hematopoietic stem/progenitor cells (HSPCs) to B‐cell lineages. Following validation of the responsiveness of the protocol to additional stimulations and the uniformity of the experimental conditions, human HSPCs were continuously exposed to 300 mT of 50 Hz MF for 35 days of the differentiation process. These experiments were performed in a blinded manner. The percentages of myeloid or lymphoid cells and their degree of differentiation from pro‐B to immature‐B cells in the MF‐exposed group showed no significant changes compared with those in the control group. Furthermore, the expression levels of recombination‐activating gene (RAG)1 and RAG2 in the B cells were also similar to those in the control group. These results indicate that exposure to 50 Hz MF at 300 mT does not affect the human B‐cell early differentiation from HSPCs. © 2023 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 5-6","pages":"119-128"},"PeriodicalIF":1.9,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22447","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9747550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Uniform and Nonuniform Magnetic Fields in Plant Growth: A Meta-Analysis Approach 均匀和非均匀磁场对植物生长的影响:一种荟萃分析方法

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-04-18 DOI: 10.1002/bem.22445

Francisco Tapia-Belmonte, Andrés Concha, María J. Poupin

引用次数: 1

Evaluation of Exposure to Radiofrequency Electromagnetic Fields from Smart Utility Meters operating at 868 MHz 868兆赫的智能电表对射频电磁场的暴露评估

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-03-31 DOI: 10.1002/bem.22440

Darren Addison, Carolina Calderon, Azadeh Peyman

引用次数: 0

Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field 暴露于900 MHz射频场后立即分析人角质形成细胞的整体DNA甲基化变化

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-03-31 DOI: 10.1002/bem.22439

Jody C. Cantu, Joseph W. Butterworth, Xomalin G. Peralta, Jason A. Payne, Ibtissam Echchgadda

{"title":"Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field","authors":"Jody C. Cantu, Joseph W. Butterworth, Xomalin G. Peralta, Jason A. Payne, Ibtissam Echchgadda","doi":"10.1002/bem.22439","DOIUrl":"10.1002/bem.22439","url":null,"abstract":"The increasing use of nonionizing radiofrequency electromagnetic fields (RF-EMFs) in a wide range of technologies necessitates studies to further understanding of biological effects from exposures to such forms of electromagnetic fields. While previous studies have described mechanisms for cellular changes occurring following exposure to low-intensity RF-EMFs, the role of molecular epigenetics has not been thoroughly investigated. Specifically unresolved is the effect of RF-EMFs on deoxyribonucleic acid (DNA) methylation, which is a powerful epigenetic process, used by cells to regulate gene expression. DNA methylation is dynamic and can be rapidly triggered in response to external stimuli such as exposure to RF-EMFs. In the present study, we performed a global analysis of DNA methylation patterns in human keratinocytes exposed to 900 MHz RF-EMFs for 1 h at a low dose rate (estimated mean specific absorption rate (SAR) < 10 mW/kg). We used a custom system to allow stable exposure of cell cultures to RF-EMFs under biologically relevant conditions (37 °C, 5% CO2, 95% humidity). We performed whole genome bisulfite sequencing directly following RF-EMF exposure to examine the immediate changes in DNA methylation patterns and identify early differentially methylated genes in RF-EMF-exposed keratinocytes. By correlating global gene expression to whole genome bisulfite sequencing, we identified six common targets that were both differentially methylated and differentially expressed in response to RF-EMF exposure. The results highlight a potential epigenetic role in the cellular response to RF-EMFs. Particularly, the six identified targets may potentially be developed as epigenetic biomarkers for immediate responses to RF-EMF exposure. Bioelectromagnetics. 1–13, © 2023 Bioelectromagnetics Society. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 3-4","pages":"77-89"},"PeriodicalIF":1.9,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9698747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0