Bioelectromagnetics最新文献_第8页

Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field 暴露于900 MHz射频场后立即分析人角质形成细胞的整体DNA甲基化变化

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-03-31 DOI: 10.1002/bem.22439

Jody C. Cantu, Joseph W. Butterworth, Xomalin G. Peralta, Jason A. Payne, Ibtissam Echchgadda

{"title":"Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field","authors":"Jody C. Cantu, Joseph W. Butterworth, Xomalin G. Peralta, Jason A. Payne, Ibtissam Echchgadda","doi":"10.1002/bem.22439","DOIUrl":"10.1002/bem.22439","url":null,"abstract":"The increasing use of nonionizing radiofrequency electromagnetic fields (RF-EMFs) in a wide range of technologies necessitates studies to further understanding of biological effects from exposures to such forms of electromagnetic fields. While previous studies have described mechanisms for cellular changes occurring following exposure to low-intensity RF-EMFs, the role of molecular epigenetics has not been thoroughly investigated. Specifically unresolved is the effect of RF-EMFs on deoxyribonucleic acid (DNA) methylation, which is a powerful epigenetic process, used by cells to regulate gene expression. DNA methylation is dynamic and can be rapidly triggered in response to external stimuli such as exposure to RF-EMFs. In the present study, we performed a global analysis of DNA methylation patterns in human keratinocytes exposed to 900 MHz RF-EMFs for 1 h at a low dose rate (estimated mean specific absorption rate (SAR) < 10 mW/kg). We used a custom system to allow stable exposure of cell cultures to RF-EMFs under biologically relevant conditions (37 °C, 5% CO2, 95% humidity). We performed whole genome bisulfite sequencing directly following RF-EMF exposure to examine the immediate changes in DNA methylation patterns and identify early differentially methylated genes in RF-EMF-exposed keratinocytes. By correlating global gene expression to whole genome bisulfite sequencing, we identified six common targets that were both differentially methylated and differentially expressed in response to RF-EMF exposure. The results highlight a potential epigenetic role in the cellular response to RF-EMFs. Particularly, the six identified targets may potentially be developed as epigenetic biomarkers for immediate responses to RF-EMF exposure. Bioelectromagnetics. 1–13, © 2023 Bioelectromagnetics Society. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 3-4","pages":"77-89"},"PeriodicalIF":1.9,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9698747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information - Page 问题信息-页面

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-03-27 DOI: 10.1002/bem.22441

引用次数: 0

Effects of neodymium magneto-priming on seed germination and salinity tolerance in tomato 磁化钕对番茄种子萌发及耐盐性的影响

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-02-17 DOI: 10.1002/bem.22438

Mohammad K. Abhary, Abdullah Akhkha

{"title":"Effects of neodymium magneto-priming on seed germination and salinity tolerance in tomato","authors":"Mohammad K. Abhary, Abdullah Akhkha","doi":"10.1002/bem.22438","DOIUrl":"10.1002/bem.22438","url":null,"abstract":"Earth's biosphere is surrounded by magnetic fields that affect all living organisms. A plant's response to magnetic fields is displayed in terms of its seed's vigor, growth, and yield. Examining seed germination in such magnetic fields is the first step in the investigation of how magnetic fields might be used to enhance plant growth and maximize crop performance. In this study, salinity-sensitive Super Strain-B tomato seeds were primed with the northern and southern poles of neodymium magnets of 150, 200, and 250 mT. The magneto-primed seeds showed a significant increase in germination rate and speed, where the orientation of the magnet was identified as being crucial for germination rate and the orientation of seeds towards the magnet was shown to affect the germination speed. The primed plants exhibited enhanced growth characteristics, including longer shoots and roots, larger leaf area, more root hairs, higher water content, and more tolerance to salinity levels, up to 200 mM NaCl. All magneto-primed plants showed a significant decrease in chlorophyll content, continuous chlorophyll fluorescence yield (Ft), and quantum yield (QY). The salinity treatments decreased all chlorophyll parameters in control plants, significantly, but did not lower such parameters in magneto-primed tomatoes. The results of this study illustrate the positive effects of neodymium magnet on the growth and development of tomato plants in terms of their germination, growth, and salinity tolerance, and negatively affected the chlorophyll content in tomato leaves. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 1-2","pages":"47-56"},"PeriodicalIF":1.9,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9551684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Importance of Subcellular Structures to the Modeling of Biological Cells in the Context of Computational Bioelectromagnetics Simulations 亚细胞结构对计算生物电磁学模拟背景下生物细胞建模的重要性

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-02-16 DOI: 10.1002/bem.22436

Kevin Jerbic, Jan T. Svejda, Benedikt Sievert, Andreas Rennings, Jürg Fröhlich, Daniel Erni

{"title":"The Importance of Subcellular Structures to the Modeling of Biological Cells in the Context of Computational Bioelectromagnetics Simulations","authors":"Kevin Jerbic, Jan T. Svejda, Benedikt Sievert, Andreas Rennings, Jürg Fröhlich, Daniel Erni","doi":"10.1002/bem.22436","DOIUrl":"10.1002/bem.22436","url":null,"abstract":"Numerical investigation of the interaction of electromagnetic fields with eukaryotic cells requires specifically adapted computer models. Virtual microdosimetry, used to investigate exposure, requires volumetric cell models, which are numerically challenging. For this reason, a method is presented here to determine the current and volumetric loss densities occurring in single cells and their distinct compartments in a spatially accurate manner as a first step toward multicellular models within the microstructure of tissue layers. To achieve this, 3D models of the electromagnetic exposure of generic eukaryotic cells of different shape (i.e. spherical and ellipsoidal) and internal complexity (i.e. different organelles) are performed in a virtual, finite element method-based capacitor experiment in the frequency range from 10 Hz to 100 GHz. In this context, the spectral response of the current and loss distribution within the cell compartments is investigated and any effects that occur are attributed either to the dispersive material properties of these compartments or to the geometric characteristics of the cell model investigated in each case. In these investigations, the cell is represented as an anisotropic body with an internal distributed membrane system of low conductivity that mimics the endoplasmic reticulum in a simplified manner. This will be used to determine which details of the cell interior need to be modeled, how the electric field and the current density will be distributed in this region, and where the electromagnetic energy is absorbed in the microstructure regarding electromagnetic microdosimetry. Results show that for 5 G frequencies, membranes make a significant contribution to the absorption losses. © 2023 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 1-2","pages":"26-46"},"PeriodicalIF":1.9,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9536527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiofrequency Exposure Levels in Greece 希腊的射频暴露水平

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-02-14 DOI: 10.1002/bem.22434

Charilaos Tyrakis, Kiki Theodorou, Yiannis Kiouvrekis, Aris Alexias, Constantin Kappas

{"title":"Radiofrequency Exposure Levels in Greece","authors":"Charilaos Tyrakis, Kiki Theodorou, Yiannis Kiouvrekis, Aris Alexias, Constantin Kappas","doi":"10.1002/bem.22434","DOIUrl":"10.1002/bem.22434","url":null,"abstract":"Medical Physics Department (Medical School, University of Thessaly) participated in a Greek National EMF research program (EDBM34) with the scope to measure and evaluate radiofrequency (RF) exposure (27–3000 MHz) in areas of sensitive land use. A thousand (1000) measurements were carried out at two “metropolitan locations” (Athens and Thessaloniki: 624 points) and several rest urban/rural locations (376 points). SRM 3006 spectrum analyzer manufactured by Narda Safety Test Solutions was used. The broadband mean electric field in metropolitan areas was 0.41 V/m, while in the rest of Greece was 0.36 V/m. In metropolitan areas, the predominant RF source was the TV and Radio FM signals (36.2% mean contribution to the total RF exposure level). In the rest areas, the predominant source was the systems of the meteorological and military/defensive service (31.1%). The mobile sector contributed 14.9% in metropolitan areas versus 12.2% in the rest of Greece. The predominant mobile source was 900 MHz in both cases (4.5% in metropolitan areas vs. 3.3% in the rest of Greece). The total exposure from all RF sources complied with the International Commission on Non-Ionizing Radiation Protection (ICNIRP) 2020 safety guidelines [ICNIRP, 2020]. The maximum exposure level was 0.129% of the limit for the metropolitan areas vs. 0.110% for the rest of Greece. Nonremarkable differences between metropolitan areas' exposure and the rest of Greece. In most cases, new 5 G antennas will be added to the existing base stations. Thus, the total exposure may be increased, leading to higher safety distances. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 1-2","pages":"17-25"},"PeriodicalIF":1.9,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9179259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Aspects of Synaptic Plasticity in Hippocampus via mT Extremely Low-Frequency Magnetic Fields 极低频磁场对海马突触可塑性的研究进展

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-02-14 DOI: 10.1002/bem.22437

Lei Dong, Pei Xia, Lei Tian, Chunxiao Tian, Wenjun Zhao, Ling Zhao, Jiakang Duan, Yuhan Zhao, Yu Zheng

{"title":"A Review of Aspects of Synaptic Plasticity in Hippocampus via mT Extremely Low-Frequency Magnetic Fields","authors":"Lei Dong, Pei Xia, Lei Tian, Chunxiao Tian, Wenjun Zhao, Ling Zhao, Jiakang Duan, Yuhan Zhao, Yu Zheng","doi":"10.1002/bem.22437","DOIUrl":"10.1002/bem.22437","url":null,"abstract":"The subthreshold magnetic modulation technique stimulates cells with mT extremely low-frequency magnetic fields (ELF-MFs), which are insufficient to induce neuronal action potentials. Although they cannot directly induce resting neurons to discharge, mT magnetic stimulation can regulate the excitability of the nervous system, which regulates learning and memory by some unknown mechanisms. Herein, we describe the regulation of mT ELF-MFs with different parameters on synaptic plasticity in hippocampal neurons. Additionally, we summarize the latest research on the possible mechanism of the effect of ELF-MFs on synaptic plasticity. Some studies have shown that ELF-MFs are able to inhibit long-term potentiation (LTP) by increasing concentration of intracellular Ca2+ concentration ([Ca2+]i), as well as concentration of reactive oxygen species. The research in this paper has significance for the comprehensive understanding of relevant neurological mechanisms of learning and memory by mT ELF-MFs stimulation. However, more high-quality research is necessary to determine the regulatory mechanism of mT ELF-MFs on synaptic plasticity in order to optimize this technique as a treatment for neurological diseases. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 3-4","pages":"63-70"},"PeriodicalIF":1.9,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9697194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of Viral Inactivation on Dry Surface by High Peak Power Microwave (HPPM) Exposure 高峰值功率微波(HPPM)对干燥表面病毒灭活的评价

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2023-02-14 DOI: 10.1002/bem.22435

Ibtissam Echchgadda, Jody C. Cantu, Joey Butterworth, Bryan Gamboa, Ronald Barnes, David A. Freeman, Francis A. Ruhr, Weston C. Williams, Leland R. Johnson, Jason Payne, Robert J. Thomas, William P. Roach, Bennett L. Ibey

{"title":"Evaluation of Viral Inactivation on Dry Surface by High Peak Power Microwave (HPPM) Exposure","authors":"Ibtissam Echchgadda, Jody C. Cantu, Joey Butterworth, Bryan Gamboa, Ronald Barnes, David A. Freeman, Francis A. Ruhr, Weston C. Williams, Leland R. Johnson, Jason Payne, Robert J. Thomas, William P. Roach, Bennett L. Ibey","doi":"10.1002/bem.22435","DOIUrl":"10.1002/bem.22435","url":null,"abstract":"Previous research has shown that virus infectivity can be dramatically reduced by radio frequency exposure in the gigahertz (GHz) frequency range. Given the worldwide SARS-CoV-2 pandemic, which has caused over 1 million deaths and has had a profound global economic impact, there is a need for a noninvasive technology that can reduce the transmission of virus among humans. RF is a potential wide area-of-effect viral decontamination technology that could be used in hospital rooms where patients are expelling virus, in grocery and convenience stores where local populations mix, and in first responder settings where rapid medical response spans many potentially infected locations within hours. In this study, we used bovine coronavirus (BCoV) as a surrogate of SARS-CoV-2 and exposed it to high peak power microwave (HPPM) pulses at four narrowband frequencies: 2.8, 5.6, 8.5, and 9.3 GHz. Exposures consisted of 2 µs pulses delivered at 500 Hz, with pulse counts varied by decades between 1 and 10,000. The peak field intensities (i.e. the instantaneous power density of each pulse) ranged between 0.6 and 6.5 MW/m2, depending on the microwave frequency. The HPPM exposures were delivered to plastic coverslips containing BCoV dried on the surface. Hemagglutination (HA) and cytopathic effect analyses were performed 6 days after inoculation of host cells to assess viral infectivity. No change in viral infectivity was seen with increasing dose (pulse number) across the tested frequencies. Under all conditions tested, exposure did not reduce infectivity more than 1.0 log10. For the conditions studied, high peak power pulsed RF exposures in the 2–10 GHz range appear ineffective as a virucidal approach for hard surface decontamination. © 2023 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"44 1-2","pages":"5-16"},"PeriodicalIF":1.9,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9179261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Issue Information - Page 发行信息-页面

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2022-12-26 DOI: 10.1002/bem.22432

引用次数: 0

Exposure to 10 Hz Pulsed Magnetic Field Induced Slight Apoptosis and Reactive Oxygen Species in Primary Human Gingival Fibroblasts 暴露于10hz脉冲磁场诱导原代人牙龈成纤维细胞轻微凋亡和活性氧

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2022-12-09 DOI: 10.1002/bem.22428

Zheng Huang, Cheng Ding, Xinzhao Huang, Chuan Sun, Liangjun Zhong

引用次数: 0

Effect of 40 Hz Magnetic Field Application in Posttraumatic Muscular Atrophy Development on Muscle Mass and Contractions in Rats 40hz磁场对创伤后肌萎缩发育大鼠肌肉质量和收缩的影响

IF 1.9 3区生物学

Bioelectromagnetics Pub Date : 2022-12-07 DOI: 10.1002/bem.22429

Figen Cicek, Bora Tastekin, Ilknur Baldan, Murat Tokus, Aykut Pelit, Isil Ocal, Ismail Gunay, Hasan U. Ogur, Hakan Cicek

{"title":"Effect of 40 Hz Magnetic Field Application in Posttraumatic Muscular Atrophy Development on Muscle Mass and Contractions in Rats","authors":"Figen Cicek, Bora Tastekin, Ilknur Baldan, Murat Tokus, Aykut Pelit, Isil Ocal, Ismail Gunay, Hasan U. Ogur, Hakan Cicek","doi":"10.1002/bem.22429","DOIUrl":"10.1002/bem.22429","url":null,"abstract":"Muscle atrophy refers to the deterioration of muscle tissue due to a long-term decrease in muscle function. In the present study, we simulated rectus femoris muscle atrophy experimentally and investigated the effect of pulsed electromagnetic field (PEMF) application on the atrophy development through muscle mass, maximal contraction force, and contraction–relaxation time. A quadriceps tendon rupture with a total tenotomy was created on the rats’ hind limbs, inhibiting knee extension for 6 weeks, and this restriction of the movement led to the development of disuse atrophy, while the control group underwent no surgery. The operated and control groups were divided into subgroups according to PEMF application (1.5 mT for 45 days) or no PEMF. All groups were sacrificed after 6 weeks and had their entire rectus femoris removed. To measure the contraction force, the muscles were placed in an organ bath connected to a transducer. As a result of the atrophy, muscle mass and strength were reduced in the operated group, while no muscle mass loss was observed in the operated PEMF group. Furthermore, measurements of single, incomplete and full tetanic contraction force and contraction time (CT) did not change significantly in the operated group that received the PEMF application. The PEMF application prevented atrophy resulting from 6 weeks of immobility, according to the contraction parameters. The effects of PEMF on contraction force and CT provide a basis for further studies in which PEMF is investigated as a noninvasive therapy for disuse atrophy development. © 2022 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 8","pages":"453-461"},"PeriodicalIF":1.9,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10434682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1