Bioelectromagnetics最新文献

{"title":"An Extremely Low-Frequency Vortex Magnetic Field Modifies Protein Expression, Rearranges the Cytoskeleton, and Induces Apoptosis of a Human Neuroblastoma Cell Line","authors":"Diana I. Aparicio-Bautista,&nbsp;Daniel Chávez-Valenzuela,&nbsp;Giovanni Ambriz-Álvarez,&nbsp;Teodoro Córdova-Fraga,&nbsp;Juan P. Reyes-Grajeda,&nbsp;Óscar Medina-Contreras,&nbsp;Fanny Rodríguez-Cruz,&nbsp;Francisco García-Sierra,&nbsp;Patricia Zúñiga-Sánchez,&nbsp;Antonio M. Gutiérrez-Gutiérrez,&nbsp;Jaime Arellanes-Robledo,&nbsp;Gustavo Basurto-Islas","doi":"10.1002/bem.22400","DOIUrl":"10.1002/bem.22400","url":null,"abstract":"<p>Homogeneous extremely low-frequency electromagnetic fields (ELF-EMFs) alter biological phenomena, including the cell phenotype and proliferation rate. Heterogenous vortex magnetic fields (VMFs), a new approach of exposure to magnetic fields, induce systematic movements on charged biomolecules from target cells; however, the effect of VMFs on living systems remains uncertain. Here, we designed, constructed, and characterized an ELF-VMF-modified Rodin's coil to expose SH-SY5Y cells. Samples were analyzed by performing 2D-differential-gel electrophoresis, identified by MALDI-TOF/TOF, validated by western blotting, and characterized by confocal microscopy. A total of 106 protein spots were differentially expressed; 40 spots were downregulated and 66 were upregulated in the exposed cell proteome, compared to the control cell proteome. The identified spots are associated with cytoskeleton and cell viability proteins, and according to the protein–protein interaction network, a significant interaction among them was found. Our data revealed a decrease in cell survival associated with apoptotic cells without effects on the cell cycle, as well as evident changes in the cytoskeleton. We demonstrated that ELF-VMFs, at a specific frequency and exposure time, alter the cell proteome and structurally affect the target cells. This is the first report showing that VMF application might be a versatile system for testing different hypotheses in living systems, using appropriate exposure parameters.© 2022 Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 4","pages":"225-244"},"PeriodicalIF":1.9,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44874657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage","authors":"Gagik Greg Haroutunian,&nbsp;Ashot Tsaghikian,&nbsp;Elena Fedorova,&nbsp;Pratima Chaurasia,&nbsp;Gabriele Luca Gusella,&nbsp;Arevik Mosoian","doi":"10.1002/bem.22401","DOIUrl":"10.1002/bem.22401","url":null,"abstract":"<p>Rapid advances in mesenchymal stem progenitor cells (MSPCs) have rendered impetus into the area of cell therapy and regenerative medicine. The main promise of future stem cell therapies is their reliance on autologous stem cells derived from adipose tissue, which also includes treatments of bone fractures and degeneration. The effectiveness of different electric devices utilized to reprogram MSPCs toward osteogenic differentiation has provided varying degrees of effectiveness for clinical use. Adipose tissue-derived MSPCs were flow-cytometrically characterized and further differentiated into osteoblasts by culturing either in growth medium with pro-osteogenic supplements or without supplements with alternating electromagnetic field (EMF) generated by IteraCoil. IteraCoil is a multi-solenoid coil with a specific complex geometry that creates a 3D-EMF with desired parameters without directly applying electrodes to the cells and tissues. The flow-cytometric analysis of highly enriched (≥95%) adipose-derived MSPCs (CD34⁻, CD73⁺, CD90⁺, and CD105⁺) was utilized for the study. Osteoblasts and chondrocyte differentiations were then assessed by specific staining and quantified using ImageJ (National Institutes of Health). The osteoblastic differentiation of MSPCs cultured in regular medium and exposed to EMF at 0.05 and 1 kHz frequencies was compared with MSPCs cultured in a pro-osteogenic supplemented medium. In this study, we demonstrated that EMF from IteraCoil might have affected the signaling pathways that induce the osteogenic differentiation of human adipose-derived MSPCs in the absence of exogenous osteogenic factors. Therefore, EMF-generated osteogenic differentiation of reprogrammed adipose-derived autologous MSPCs may treat the loss of osteoblasts and osteoporosis and open new avenues for the development of regenerative cellular therapy. © 2022 Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 4","pages":"245-256"},"PeriodicalIF":1.9,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49403616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Red Blood Cell Movement in Whole Blood Exposed to DC and ELF Electric Fields","authors":"Miki Kanemaki,&nbsp;Hisae O. Shimizu,&nbsp;Hiroshi Inujima,&nbsp;Takeo Miyake,&nbsp;Koichi Shimizu","doi":"10.1002/bem.22395","DOIUrl":"10.1002/bem.22395","url":null,"abstract":"<p>To evaluate hematological effects of direct current (DC) and alternating current (AC) extremely low frequency (ELF) electric field exposure, this study investigated red blood cell (RBC) movement in whole blood. Video images of RBCs were recorded under a microscope using specially designed electrode systems. Video analysis software was then used to measure the RBC velocity. The noise level and measurement system stability were confirmed based on results of a no-field exposure experiment. Using the electrode system to produce a non-homogeneous electric field, different movements were found to occur in DC and AC field exposure. The RBCs moved in the directions of the electric field and the gradient of field distribution, respectively, in the DC and AC fields. Dependences of the RBC velocity on the field strength were, respectively, linear and quadratic in the DC and AC fields. These results suggest that electrophoretic and dielectrophoretic movements were, respectively, dominant in the DC and AC fields. The magnitude of the electric field necessary to cause these effects was found to be 10³–10⁵ times greater than the internationally publicized guideline for human safety. © 2022 Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 3","pages":"149-159"},"PeriodicalIF":1.9,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/15/c7/BEM-43-149.PMC9313574.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40312202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information - Page","authors":"","doi":"10.1002/bem.22398","DOIUrl":"https://doi.org/10.1002/bem.22398","url":null,"abstract":"","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 3","pages":"145-147"},"PeriodicalIF":1.9,"publicationDate":"2022-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92306291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Twin Fetal Exposure to Environmental Magnetic and Electromagnetic Fields","authors":"Chen Zhang,&nbsp;Congsheng Li,&nbsp;Lei Yang,&nbsp;Wenjing Hou,&nbsp;Mingshan Du,&nbsp;Tongning Wu,&nbsp;Wei Chen","doi":"10.1002/bem.22397","DOIUrl":"10.1002/bem.22397","url":null,"abstract":"<p>Fetal development is vital in the human lifespan. Therefore, it is essential to characterize exposure by a series of typical environmental magnetic and electromagnetic fields. In particular, there has recently been a sharp increase in the twin birth rate. However, lack of appropriate models has prohibited dosimetric evaluation, restricting characterization of the impact of these environmental factors on twins. The present study developed two whole-body pregnant models of 31 and 32 weeks of gestation with twin fetuses and explored several typical exposure scenarios, including 50-Hz uniform magnetic field exposure, local 125-kHz magnetic field (MF), and 13.56-MHz electromagnetic field exposure, as well as wideband planewave radiofrequency (RF) exposure from 20 to 6000 MHz. Finally, dosimetric results were derived. Compared to the singleton pregnancy with similar weeks of gestation, twin fetuses were overexposed at 50-Hz uniform MF, but they were probably underexposed in the RF scenarios with frequencies for wireless communications. Furthermore, the twin fetuses manifested large dosimetric variability compared to the singleton, which was attributed to the incident direction and fetal position. Based on the analysis, the dosimetric results over the entire gestation period were estimated. The results can be helpful to estimate the risk of twin-fetal exposure to electromagnetic fields and examine the conservativeness of the international guidelines.© 2022 Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 3","pages":"160-173"},"PeriodicalIF":1.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44817119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adjunctive Diagnostic Methods for Skin Cancer Detection: A Review of Electrical Impedance-Based Techniques","authors":"U. Anushree,&nbsp;Sachin Shetty,&nbsp;Rajesh Kumar,&nbsp;Sanjay Bharati","doi":"10.1002/bem.22396","DOIUrl":"10.1002/bem.22396","url":null,"abstract":"Skin cancer is among the fastest-growing cancers with an excellent prognosis, if detected early. However, the current method of diagnosis by visual inspection has several disadvantages such as overlapping tumor characteristics, subjectivity, low sensitivity, and specificity. Hence, several adjunctive diagnostic techniques such as thermal imaging, optical imaging, ultrasonography, tape stripping methods, and electrical impedance imaging are employed along with visual inspection to improve the diagnosis. Electrical impedance-based skin cancer detection depends upon the variations in electrical impedance characteristics of the transformed cells. The information provided by this technique is fundamentally different from other adjunctive techniques and thus has good prospects. Depending on the stage, type, and location of skin cancer, various impedance-based devices have been developed. These devices when used as an adjunct to visual methods have increased the sensitivity and specificity of skin cancer detection up to 100% and 87%, respectively, thus demonstrating their potential to minimize unnecessary biopsies. In this review, the authors track the advancements and progress made in this technique for the detection of skin cancer, focusing mainly on the advantages and limitations in the clinical setting. © 2022 Bioelectromagnetics Society.","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 3","pages":"193-210"},"PeriodicalIF":1.9,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39645183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effects of Exposure to Power-Frequency Magnetic Fields on the Differentiation of Hematopoietic Stem/Progenitor Cells Using Human-Induced Pluripotent Stem Cells","authors":"Masayuki Takahashi,&nbsp;Naoko Furuya","doi":"10.1002/bem.22394","DOIUrl":"10.1002/bem.22394","url":null,"abstract":"<p>The causal association between exposure to power-frequency magnetic fields (MFs) and childhood leukemia has been under discussion. Although evidence from experimental studies is required for a conclusion to be reached, only a few studies have focused on the effects of MF exposure on the human hematopoietic system directly related to leukemogenesis. Here, we established an in vitro protocol to simulate the differentiation of human mesodermal cells to hematopoietic stem progenitor cells (HSPCs) using human-induced pluripotent stem cells. Furthermore, we introduced MF in the protocol to study the effects of exposure. After a continuous exposure to 0–300 mT of 50-Hz MFs during the differentiation process, the efficiency of differentiation of mesodermal cells into HSPCs was analyzed in a single-blinded manner. The percentage of emerged HSPCs from mesodermal cells in groups exposed to 50-Hz MFs indicated a lack of significant changes compared with those in the sham-exposed group. These results suggest that exposure to 50-Hz MFs up to 300 mT does not affect the differentiation of human mesodermal cells to HSPCs, which may be involved in the initial process of leukemogenesis. © 2022 The Authors. Bioelectromagnetics published by Wiley Periodicals LLC on behalf of Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 3","pages":"174-181"},"PeriodicalIF":1.9,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c2/6d/BEM-43-174.PMC9304145.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39602545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-Field Exposure in FM Frequencies: New Methodology and Estimation Formulas","authors":"Bader Mustafa Fetouri PhD,&nbsp;Soumaya Azzi PhD,&nbsp;Allal Ouberehil,&nbsp;Philippe Briend,&nbsp;Philippe De Doncker PhD,&nbsp;Joe Wiart PhD","doi":"10.1002/bem.22391","DOIUrl":"10.1002/bem.22391","url":null,"abstract":"<p>Workers inside transmission pylons with FM antenna arrays are likely to be exposed to near-field radiation exceeding reference levels for occupational exposure. In this study, the near-field behavior of 64 FM pylons was studied using a new methodology. Near-field characterization was done using field metrics without taking into account field sources' size or distance from field source. The specific absorption rate (SAR) was assessed in five hundred different near-field cases using a human phantom. Estimation formulas for both local and whole-body SAR are provided and validated numerically. Local and whole-body SAR are linked to electric field strength. © 2022 Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 3","pages":"182-192"},"PeriodicalIF":1.9,"publicationDate":"2022-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39870975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information - Page","authors":"","doi":"10.1002/bem.22392","DOIUrl":"10.1002/bem.22392","url":null,"abstract":"","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 2","pages":"65-67"},"PeriodicalIF":1.9,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22392","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43881614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chain-Like Structures of Biogenic and Nonbiogenic Magnetic Nanoparticles in Vascular Tissues","authors":"Svitlana Gorobets,&nbsp;Oksana Gorobets,&nbsp;Yuri Gorobets,&nbsp;Maryna Bulaievska","doi":"10.1002/bem.22390","DOIUrl":"10.1002/bem.22390","url":null,"abstract":"<div>\u0000 <section>\u0000 <p>In this paper, slices of organs from various organisms (animals, plants, fungi) were investigated by using atomic force microscopy and magnetic force microscopy to identify common features of localization of both biogenic and nonbiogenic magnetic nanoparticles. It was revealed that both biogenic and nonbiogenic magnetic nanoparticles are localized in the form of chains of separate nanoparticles or chains of conglomerates of nanoparticles in the walls of the capillaries of animals and the walls of the conducting tissue of plants and fungi. Both biogenic and nonbiogenic magnetic nanoparticles are embedded as a part of the transport system in multicellular organisms. In connection with this, a new idea of the function of biogenic magnetic nanoparticles is discussed, that the chains of biogenic magnetic nanoparticles and chains of conglomerates of biogenic magnetic nanoparticles represent ferrimagnetic organelles of a specific purpose. Besides, magnetic dipole–dipole interaction of biogenic magnetic nanoparticles with magnetically labeled drugs or contrast agents for magnetic resonance imaging should be considered when designing the drug delivery and other medical systems because biogenic magnetic nanoparticles in capillary walls will serve as the trapping centers for the artificial magnetic nanoparticles. The aggregates of both artificial and biogenic magnetic nanoparticles can be formed, contributing to the risk of vascular occlusion. Bioelectromagnetics. 43:119–143, 2022. © 2021 Bioelectromagnetics Society.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 2","pages":"119-143"},"PeriodicalIF":1.9,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39858630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}