Bioelectromagnetics最新文献

{"title":"Protective effect of radiofrequency exposure against menadione-induced oxidative DNA damage in human neuroblastoma cells: The role of exposure duration and investigation on key molecular targets","authors":"Anna Sannino PhD,&nbsp;Mariateresa Allocca PhD,&nbsp;Maria R. Scarfì MSc,&nbsp;Stefania Romeo PhD,&nbsp;Olga Zeni PhD","doi":"10.1002/bem.22524","DOIUrl":"10.1002/bem.22524","url":null,"abstract":"<p>In our previous studies, we demonstrated that 20 h pre-exposure of SH-SY5Y human neuroblastoma cells to 1950 MHz, UMTS signal, at specific absorption rate of 0.3 and 1.25 W/kg, was able to reduce the oxidative DNA damage induced by a subsequent treatment with menadione in the alkaline comet assay while not inducing genotoxicity per se. In this study, the same cell model was used to test the same experimental conditions by setting different radiofrequency exposure duration and timing along the 72 h culture period. The results obtained in at least three independent experiments indicate that shorter exposure durations than 20 h, that is, 10, 3, and 1 h per day for 3 days, were still capable to exert the protective effect while not inducing DNA damage per se. In addition, to provide some hints into the mechanisms underpinning the observed phenomenon, thioredoxin-1, heat shock transcription factor 1, heat shock protein 70, and poly [ADP-ribose] polymerase 1, as key molecular players involved in the cellular stress response, were tested following 3 h of radiofrequency exposure in western blot and qRT-PCR experiments. No effect resulted from molecular analysis under the experimental conditions adopted.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 8","pages":"365-374"},"PeriodicalIF":1.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed electromagnetic fields attenuate human musculocutaneous nerve damage induced by biceps eccentric contractions","authors":"Karina Kouzaki PhD,&nbsp;Koichi Nakazato PhD","doi":"10.1002/bem.22525","DOIUrl":"10.1002/bem.22525","url":null,"abstract":"<p>Pulsed electromagnetic field (PEMF) therapy, a noninvasive treatment, has shown promise in mitigating nerve damage. However, unaccustomed exercises, such as eccentric contractions (ECCs), can damage both muscle and nerve tissue. This study investigated whether magnetic stimulation (MS) with PEMF could aid in nerve recovery after ECCs in the elbow flexors. Twenty participants were randomly assigned to either a control (CNT) or an MS group. Following ECCs, we measured the latency of the M-wave in the musculocutaneous nerve as an indicator of nerve function. Additionally, isometric torque, range of motion, and muscle pain were assessed for muscle function. Interestingly, only the CNT group exhibited a significant increase in latency on Day 2 (<i>p</i> &lt; 0.05). The MS group, on the other hand, displayed an earlier recovery trend in isometric torque, range of motion, and muscle soreness. Notably, muscle soreness significantly decreased immediately after MS treatment compared to pretreatment levels. These findings suggest that MS treatment can effectively attenuate nerve damage induced by ECCs exercise.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"46 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of simulated electric fields of transcranial magnetic stimulation targeting different cortical motor regions","authors":"Jack Jiaqi Zhang PhD,&nbsp;Bella Bingbing Zhang MSc,&nbsp;Zhongfei Bai PhD,&nbsp;Kenneth N. K. Fong PhD","doi":"10.1002/bem.22523","DOIUrl":"10.1002/bem.22523","url":null,"abstract":"<p>This computational simulation study investigates the strength of transcranial magnetic stimulation (TMS)-induced electric fields (EF) in primary motor cortex (M1) and secondary motor areas. Our results reveal high interindividual variability in the strength of TMS-induced EF responses in secondary motor areas, relative to the stimulation threshold in M1. Notably, the activation of the supplementary motor area requires high-intensity stimulation, which could be attributed to the greater scalp-to-cortex distance observed over this area. These findings emphasize the importance of individualized planning using computational simulation for optimizing neuromodulation strategies targeting the cortical motor system.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"46 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinearities and timescales in neural models of temporal interference stimulation","authors":"Tom Plovie MSc,&nbsp;Ruben Schoeters PhD,&nbsp;Thomas Tarnaud PhD,&nbsp;Wout Joseph PhD,&nbsp;Emmeric Tanghe PhD","doi":"10.1002/bem.22522","DOIUrl":"10.1002/bem.22522","url":null,"abstract":"&lt;p&gt;In temporal interference (TI) stimulation, neuronal cells react to two interfering sinusoidal electric fields with a slightly different frequency (&lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 \u0000 &lt;mn&gt;1&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt; ${f}_{1}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt;, &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 \u0000 &lt;mn&gt;2&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt; ${f}_{2}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt; in the range of about 1–4 kHz, &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;mo&gt;∣&lt;/mo&gt;\u0000 \u0000 &lt;msub&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 \u0000 &lt;mn&gt;1&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 \u0000 &lt;mo&gt;−&lt;/mo&gt;\u0000 \u0000 &lt;msub&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 \u0000 &lt;mn&gt;2&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 \u0000 &lt;mo&gt;∣&lt;/mo&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt; $| {f}_{1}-{f}_{2}| $&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt; in the range of about 1–100 Hz). It has been previously observed that for the same input intensity, the neurons do not react to a purely sinusoidal signal at &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 \u0000 &lt;mn&gt;1&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt; ${f}_{1}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt; or &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 \u0000 &lt;mrow&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 \u0000 &lt;mn&gt;2&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt; ${f}_{2}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt;. This study seeks a better understanding of the largely unknown mechanisms underlying TI neuromodulation. To this end, single-compartment models are used to simulate computationally the response of neurons to the sinusoidal and TI waveform. This study compares five different neuron models: Hodgkin-Huxley (HH), Frankenhaeuser–Huxley (FH),","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"46 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulus effects of extremely low-frequency electric field exposure on calcium oscillations in a human cortical spheroid","authors":"Atsushi Saito PhD,&nbsp;Takeo Shiina PhD,&nbsp;Yoichi Sekiba PhD","doi":"10.1002/bem.22521","DOIUrl":"10.1002/bem.22521","url":null,"abstract":"<p>High-intensity, low-frequency (1 Hz to 100 kHz) electric and magnetic fields (EF and MF) cause electrical excitation of the nervous system via an induced EF (iEF) in living tissue. However, the biological properties and thresholds of stimulus effects on synchronized activity in a three-dimensional (3D) neuronal network remain uncertain. In this study, we evaluated changes in neuronal network activity during extremely low-frequency EF (ELF-EF) exposure by measuring intracellular calcium ([Ca²⁺]_<i>i</i>) oscillations, which reflect neuronal network activity. For ELF-EF exposure experiments, we used a human cortical spheroid (hCS), a 3D-cultured neuronal network generated from human induced pluripotent stem cell (hiPSC)-derived cortical neurons. A 50 Hz sinusoidal ELF-EF exposure modulated [Ca²⁺]_<i>i</i> oscillations with dependencies on exposure intensity and duration. Based on the experimental setup and results, the iEF distribution inside the hCS was estimated using high-resolution numerical dosimetry. The numerical estimation revealed threshold values ranging between 255–510 V/m (peak) and 131–261 V/m (average). This indicates that thresholds of neuronal excitation in the hCS were equivalent to those of a thin nerve fiber.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"46 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22521","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A real-time working memory evaluation system for macaques in microwave fields","authors":"Bowen Li MSc,&nbsp;Xueyan Zhang PhD,&nbsp;Nan Qiao MSc,&nbsp;Jiawei Chen MSc,&nbsp;Weijie Bi,&nbsp;Weijia Zhi PhD,&nbsp;Lizhen Ma PhD,&nbsp;Congcong Miao,&nbsp;Lifeng Wang PhD,&nbsp;Yong Zou PhD,&nbsp;Xiangjun Hu PhD","doi":"10.1002/bem.22519","DOIUrl":"10.1002/bem.22519","url":null,"abstract":"<p>With the development and widespread application of electromagnetic technology, the health hazards of electromagnetic radiation have attracted much attention and concern. The effect of electromagnetic radiation on the nervous system, especially on learning, memory, and cognitive functions, is an important research topic in the field of electromagnetic biological effects. Most previous studies were conducted with rodents, which are relatively mature. As research has progressed, studies using non-human primates as experimental subjects have been carried out. Compared to rodents, non-human primates such as macaques not only have brain structures more similar to those of humans but also exhibit learning and memory processes that are similar. In this paper, we present a behavioral test system for the real-time evaluation of the working memory (WM) of macaques in a microwave environment. The system consists of two parts: hardware and software. The hardware consists of four modules: the operation terminal, the control terminal, the optical signal transmission, and detection module and the reward feedback module. The software program can implement the feeding learning task, the button-pressing learning task, and the delayed match-to-sample task. The device is useful for the real-time evaluation of the WM of macaques in microwave environments, showing good electromagnetic compatibility, a simple and reliable structure, and easy operation.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 7","pages":"338-347"},"PeriodicalIF":1.8,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimates and measurements of radiofrequency exposures in smart-connected homes","authors":"Kenneth Joyner PhD,&nbsp;Michael Milligan LLM,&nbsp;Phillip Knipe PhD","doi":"10.1002/bem.22518","DOIUrl":"10.1002/bem.22518","url":null,"abstract":"<p>The aim of this research was to quantify the levels of radiofrequency electromagnetic energy (RF-EME) in a residential home/apartment equipped with a range of wireless devices, often referred to as internet of things (IoT) devices or smart devices and subsequently develop a tool that could be useful for estimating the levels of RF-EME in a domestic environment. Over the course of 3 years measurements were performed in peoples' homes on a total of 43 devices across 16 device categories. Another 12 devices were measured in detail in a laboratory setup. In all a total of 55 individual devices across 23 device categories were measured. Based on this measurement data we developed predictive software that showed that even with a single device in 23 device categories operating near maximum they would, in total, produce exposures at a distance of 1 m of 0.17% of the ICNIRP (2020) public exposure limits. Measurements were also made in two separate smart apartments—one contained over 50 IoT devices and a second with over 100 IoT devices with the devices driven as hard as could reasonably be achieved. The respective 6-min average exposure level recorded were 0.0077% and 0.44% of the ICNIRP (2020) 30-min average public exposure limit.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 7","pages":"329-337"},"PeriodicalIF":1.8,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141730994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional response of primary hippocampal neurons following exposure to 3.0 GHz radiofrequency electromagnetic fields","authors":"Jody C. Cantu PhD,&nbsp;Joseph W. Butterworth PhD,&nbsp;Jason A. Payne MSc,&nbsp;Ibtissam Echchgadda PhD","doi":"10.1002/bem.22517","DOIUrl":"10.1002/bem.22517","url":null,"abstract":"<p>Exposure to radiofrequency (RF) electromagnetic fields (EMF) has been associated with the modulation of neuronal electrophysiology and synaptic plasticity. Given the potential of these changes to coincide with alterations in gene expression, this study investigated whether a transcriptional response would occur in neurons following exposure to RF-EMF, under both thermal and nonthermal conditions. Rat primary hippocampal neurons (PHNs) underwent either a single (one-time) or a multiple (3-times, once a day) exposures to RF-EMF (3.0 GHz, CW) at two different mean specific absorption rate (SAR) values of 0.57 W/kg or 5.91 W/kg, which induced a temperature change (Δ<i>T</i> °C) of approximately 0.3°C or 3.6°C, respectively. Alteration in transcription in the RF-EMF-exposed PHNs versus the sham counterparts was assessed at 0, 4, and 24 h postexposure via high-throughput RNA sequencing using Illumina HiSeq. 2000. A total of 20 differentially expressed genes (DEGs) exhibited significant upregulation due to RF-EMF exposure, observed only with the high SAR dose that induced a thermal rise. However, the expression of these DEGs was not significant at 24 h postexposure. Our findings confirmed a lack of nonthermal effects on gene expression under low RF-EMF exposure conditions as evaluated. Additionally, the results indicated a slight thermal effect of exposures at the dose nearing the standards threshold of 4 W/kg; however, the effect appeared to be transient. The study suggests that RF-EMF exposures at a level close to the standards threshold, despite inducing mild temperature elevations (i.e., 3–5°C above normal), would not trigger biologically critical cellular changes.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 7","pages":"348-362"},"PeriodicalIF":1.8,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure assessment and cytogenetic biomonitoring study of workers occupationally exposed to extremely low-frequency magnetic fields","authors":"Ha Nguyen PhD,&nbsp;Giovani Vandewalle MD,&nbsp;Birgit Mertens PhD,&nbsp;Jean-Francois Collard PhD,&nbsp;Maurice Hinsenkamp MD, PhD,&nbsp;Luc Verschaeve PhD,&nbsp;Veronique Feipel PhD,&nbsp;Isabelle Magne PhD,&nbsp;Martine Souques PhD,&nbsp;Véronique Beauvois IR,&nbsp;Maryse Ledent MPE","doi":"10.1002/bem.22506","DOIUrl":"10.1002/bem.22506","url":null,"abstract":"<p>Human cytogenetic biomonitoring (HCB) has long been used to evaluate the potential effects of work environments on the DNA integrity of workers. However, HCB studies on the genotoxic effects of occupational exposure to extremely low-frequency electromagnetic fields (ELF-MFs) were limited by the quality of the exposure assessment. More specifically, concerns were raised regarding the method of exposure assessment, the selection of exposure metrics, and the definition of exposure group. In this study, genotoxic effects of occupational exposure to ELF-MFs were assessed on peripheral blood lymphocytes of 88 workers from the electrical sector using the comet and cytokinesis-block micronucleus assay, considering workers' actual exposure over three consecutive days. Different methods were applied to define exposure groups. Overall, the summarized ELF-MF data indicated a low exposure level in the whole study population. It also showed that relying solely on job titles might misclassify 12 workers into exposure groups. We proposed combining hierarchical agglomerative clustering on personal exposure data and job titles to define exposure groups. The final results showed that occupational MF exposure did not significantly induce more genetic damage. Other factors such as age or past smoking rather than ELF-MF exposure could affect the cytogenetic test outcomes.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 6","pages":"260-280"},"PeriodicalIF":1.8,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed electromagnetic fields used in regenerative medicine: An in vitro study of the skin wound healing proliferative phase","authors":"Léa Bedja-Iacona MSc,&nbsp;Riccardo Scorretti PhD,&nbsp;Marie Ducrot MSc,&nbsp;Christian Vollaire PhD,&nbsp;Laure Franqueville PhD","doi":"10.1002/bem.22508","DOIUrl":"10.1002/bem.22508","url":null,"abstract":"<p>Numerous studies have demonstrated the efficacy of extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) in accelerating the wound healing process in vitro and in vivo. Our study focuses specifically on ELF-PEMF applied with the Magnomega® device and aims to assess their effect during the main stages of the proliferative phase of dermal wound closure, in vitro. Thus, after the characterization of the EMFs delivered by the Magnomega® unit, primary culture of human dermal fibroblasts (HDFs) were exposed, or not for the control culture, to 10–12 and 100 Hz ELF-PEMF. These parameters are used in clinical practice by physiotherapists in order to enhance healing of dermal lesions in patients. HDFs proliferation was first assessed and revealed an increase in the expression of one of the two genetic markers of cell proliferation tested (PCNA and MKI67), after initial exposure of the cells to 10–12 Hz PEMF. Next, migration of HDFs was investigated by performing scratch assays on HDF layers. The observed wound closure kinetics corroborate the early organization of actin stress fibers that was revealed in the cytoplasm of HDFs exposed to 100 Hz ELF-PEMF. Also, maturation of HDFs into myofibroblasts was significantly increased in cells exposed to 10–12 or to 100 Hz PEMF. The present study is the first to demonstrate, in vitro, an early stimulation of HDFs, after their exposure to ELF-PEMF delivered by the Magnomega® device, which could contribute to an acceleration of the wound healing process.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"45 6","pages":"293-309"},"PeriodicalIF":1.8,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bem.22508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141160149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}