{"title":"Genome-guided elucidation of the biochemical mechanism driving dibenzothiophene mineralization by Bacillus safensis DH-7","authors":"Pankaj Kumar Arora, Akansha Garg, Rishabh Anand Omar, Alok Srivastava","doi":"10.1016/j.biteb.2026.102783","DOIUrl":"10.1016/j.biteb.2026.102783","url":null,"abstract":"<div><div>A desulfurizing bacterium, designated as strain DH-7, was isolated from oil-contaminated soil near Indian Oil, Panki, Kanpur, India. Based on 16S rRNA gene sequencing, phylogenetic analysis, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH), the strain was identified as <em>Bacillus safensis</em> (GenBank accession no. OQ119576). Strain DH-7 exhibited high DBT-degrading potential, completely mineralizing dibenzothiophene (DBT) within 36 h without the accumulation of any intermediate metabolite. Gas Chromatography–Mass Spectrometry (GC–MS) analysis revealed transient formation of dibenzothiophene-5-oxide, dibenzothiophene-5,5-dioxide, and benzoate as intermediates, suggesting an alternative degradation pathway distinct from the classical 4S pathway. Genome sequencing of DH-7 revealed a 3.64 Mb genome with 41.5% GC content, 3862 coding sequences, and several sulfur metabolism-related genes, including <em>cysP</em>, <em>cysT</em>, <em>cysW</em>, and <em>cysA</em>. Genes encoding alkanesulfonate monooxygenase and flavin-dependent monooxygenase were also detected, indicating their involvement in DBT degradation. The absence of desulfurization (<em>dsz)</em> genes and 2-hydroxybiphenyl (2HBP) production suggests that <em>Bacillus safensis</em> DH-7 utilizes a non-4S pathway for DBT degradation. These findings highlight DH-7 as a promising candidate for bio desulfurization and bioremediation of sulfur-containing hydrocarbons.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102783"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation and characterization of indigenous yeast strains isolated from ethnic drinks of Tripura for bioethanol production","authors":"Chandrani Debnath, Shubhankar Debnath, Sreelekha Rangubhatla, Tarun Kanti Bandyopadhyay, Muthusivaramapandian Muthuraj","doi":"10.1016/j.biteb.2026.102782","DOIUrl":"10.1016/j.biteb.2026.102782","url":null,"abstract":"<div><div>Bioethanol is gaining importance as a sustainable fuel, particularly in countries like India, where ethanol blending is mandated. The study involved isolation, screening, and characterization of the strains from Tripura's traditional ethnic drink for ethanol production under aerobic and anaerobic conditions. The study identified two non-<em>Saccharomyces</em> yeast strains, <em>Candida sojae</em> TEDI1 and <em>Candida parapsilosis</em> TEDI2, from the ethnic drink, which essentially contributed to ethanol production. The strains depicted high ethanol tolerance up to 18% (<em>v</em>/v), which is significantly higher than that of industrial ethanol producer strains. The strains also actively utilize complex sugars such as cellulose and have the potential to utilize pentose sugars. The strains depicted ethanol yield of 0.40 ± 0.015 g/g and 0.44 ± 0.01 g/g, respectively, in the presence of starch as a carbon source under anaerobic conditions. The strains showed a maximum ethanol yield of 0.120 ± 0.002 g/g and 0.149 ± 0.005 g/g, respectively, using sugarcane bagasse. The strains TEDI1 and TEDI2 showed 0.07 ± 0.01 g/g and 0.08 ± 0.01 g/g, respectively, when evaluated for ethanol yield using acid-hydrolysed biomass of <em>Chlorella lewinii</em> FWC1. Thus, the study proposes these indigenous strains as potential sources for bioethanol production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102782"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioelectrochemically assisted constructed wetlands for municipal wastewater treatment and the suitability of different biochar materials as system media","authors":"Laura Tarvainen, Katharina Kujala, Hellen Silva Santos, Maarit Liimatainen, Elisangela Heiderscheidt","doi":"10.1016/j.biteb.2026.102746","DOIUrl":"10.1016/j.biteb.2026.102746","url":null,"abstract":"<div><div>Microbial electrochemical technology constructed wetlands (METlands) use electroconductive (EC) materials in place of inert substrates such as gravel to enhance pollutant removal. This study evaluated whether EC biochar produced from waste biomass (olive pit, wood pellet, and woodchip) could function as an alternative METland media, an area with limited prior research. Biochars were tested in up-flow columns and compared with coke, a commonly used high-EC medium, and gravel, the typical inert substrate in conventional wetlands. Pollutant removal performance, material properties, biofilm-associated bacterial communities, and gas fluxes were assessed. Biochar and coke generally achieve higher removal of chemical oxygen demand (COD), nitrogen, phosphorus, and selected micropollutants than gravel, indicating that EC biochar can serve as a suitable METland medium. Electroactive bacteria were present at low relative abundance but were highest in biochar systems. Biochar columns also showed higher CO₂, N₂O, and CH₄ fluxes, though these patterns are only indicative due to limited GHG measurements, and further research is needed to clarify greenhouse gas emissions.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102746"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Payal M. Yelne, Jayeshkumar S. Mevada, Aniruddha B. Pandit
{"title":"Hydrodynamic cavitation facilitated valorisation of waste wool","authors":"Payal M. Yelne, Jayeshkumar S. Mevada, Aniruddha B. Pandit","doi":"10.1016/j.biteb.2026.102766","DOIUrl":"10.1016/j.biteb.2026.102766","url":null,"abstract":"<div><div>Efficient hydrolysis of keratin-rich wool waste is limited by its highly crosslinked protein structure, requiring high chemical and energy inputs in conventional processes. This study introduces hydrodynamic cavitation (HC) as the primary process-intensification strategy for wool hydrolysis, combined with alkali–thermal treatment, and compares its performance with conventional alkali–thermal hydrolysis. Alkali–thermal hydrolysis using 1.2% (w/v) KOH, 6% (w/v) wool loading, and heating at 121 °C for 45 min was used as the conventional reference. In contrast, the HC-assisted process employed a higher wool loading (10% w/v), a shorter thermal treatment of 10 min, followed by HC at 5 bar for 60 passes. The HC-assisted process increased wool dissolution from 70% to 95.8%. Protein extraction (Bradford assay) increased from 40% to 65.2%, and protein recovery (Kjeldahl method) increased from 46.6% to 75.1%. The increase in free thiol content from 6.8 to 16.7 mM·g<sup>−1</sup> confirmed extensive disulfide bond cleavage. Compared to the thermal alkali hydrolysis process, using HC as process intensification reduced alkali consumption from 0.20 to 0.12 kg KOH/kg wool, reduced the energy requirement from 5 kWh to 3.817 kWh for processing of 1 kg of wool and improved protein recovery, highlighting HC as a sustainable and novel intensification route for wool waste valorisation.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102766"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148499339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raquel Coldibelli Ribeiro, Elba Pinto da Silva Bon, Claudia Moraes de Rezende, Ricardo Sposina Sobral Teixeira
{"title":"Twin-screw extrusion pretreatment of defective Arabica coffee press cake for carbohydrates recovery","authors":"Raquel Coldibelli Ribeiro, Elba Pinto da Silva Bon, Claudia Moraes de Rezende, Ricardo Sposina Sobral Teixeira","doi":"10.1016/j.biteb.2026.102845","DOIUrl":"10.1016/j.biteb.2026.102845","url":null,"abstract":"<div><div>Defective Arabica coffee beans generate large amounts of low-value residues, such as coffee press cake (a byproduct of coffee oil extraction) rich in polysaccharides — mainly mannan — but still underexploited as a raw material. In this study, defective coffee press cake was processed by twin-screw extrusion followed by enzymatic hydrolysis using a crude mannanase-rich extract to recover monosaccharides. To the best of our knowledge, this is the first study to apply extrusion pretreatment optimization to defective coffee beans aiming to enhance the recovery of fermentable monosaccharides. The extrusion pretreatment was optimized using response surface methodology, identifying the best conditions as 80 °C and 20 rpm. Increasing the number of extrusion cycles enhanced enzymatic accessibility, yielding 116.2 ± 0.5 mg.g<sup>−1</sup> of mannose after 5 cycles and enabling up to 50% mannose recovery within 24 h of hydrolysis. Under optimized enzymatic conditions (40 IU.g<sup>−1</sup> enzyme loading, 48 h), mannose recovery increased to 64% relative to acid hydrolysis, corresponding to 147.1 ± 3.9 mg.g<sup>−1</sup>. At high solids loading (30%), concentrated mannose syrups containing up to 33.8% mannose were produced. Overall, this integrated process demonstrates the feasibility of valorizing defective coffee residues through a sustainable approach aligned with biorefinery and green chemistry principles, generating mannose-rich syrups with potential applications in the food, pharmaceutical, and cosmetic industries.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102845"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148499432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ararsa D. Seboka, John Morken, Muyiwa S. Adaramola, Gebresilassie A. Ewunie, Lu Feng
{"title":"Performance evaluation of leachate-recirculated solid-state anaerobic digestion of lignocellulosic biomass and digestate valorization","authors":"Ararsa D. Seboka, John Morken, Muyiwa S. Adaramola, Gebresilassie A. Ewunie, Lu Feng","doi":"10.1016/j.biteb.2026.102846","DOIUrl":"10.1016/j.biteb.2026.102846","url":null,"abstract":"<div><div>Solid-state anaerobic digestion (SS-AD) offers a promising route for treating lignocellulosic biomass, yet challenges remain regarding process stability, limited understanding of microbial dynamics under leachate recirculation, and insufficient integration of digestate valorization pathways. This study investigated the thermophilic co-digestion of coffee husk (CH) with avocado and flower wastes at proportions of 90:5:5 (CH + 10%) and 80:10:10 (CH + 20%) on a dry weight basis, using a leachate-recirculated SS-AD system for biogas production, while the resulting digestate was utilized for bio-briquette. The 20% co-substrate mixture (CH + 20%) produced the highest methane yield at 263 mL CH<sub>4</sub> g<sup>−1</sup> VS, representing a 40% improvement over mono-digested CH. The recirculated leachate maintained relatively stable pH (7.6–8.1), supported hydrolysis and prevented volatile fatty acid buildup, thereby maintaning methanogenic activity. Microbial analysis indicated that the enrichment of <em>Firmicutes</em> and <em>Proteobacteria</em> fermenters, together with <em>Methanosarcina</em>-dominated acetoclastic‑hydrogenotrophic methanogenesis, improved system stability during the co-digestion process. The BMP tests demonstrated that CH + 20% showed the highest methane production at 390 NmL CH₄ g<sup>−1</sup> VS. The digestate generated from SS-AD was subsequently valorized into bio-briquettes, producing calorific values of 16.4–17.1 MJ kg<sup>−1</sup> and improved mechanical properties that meet solid biofuel standards. This integrated approach enhances overall energy recovery from biomass residues, demonstrating the effectiveness of leachate-recirculated SS-AD for circular bioresource management.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102846"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148499438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A synthetic community derived from marine microplastic biofilms for efficient nitrate removal","authors":"Meng Sun, Weipeng Zhang, Wei Ding","doi":"10.1016/j.biteb.2026.102795","DOIUrl":"10.1016/j.biteb.2026.102795","url":null,"abstract":"<div><div>Microbial communities on marine microplastics form a novel niche and can be explored for industrially useful strains, including those for wastewater denitrification. Here, we isolated three strains (<em>P. stutzeri</em> P5F4–3, <em>Ruegeria</em> sp. PMMA7, and <em>Shimia</em> sp. pe10) carrying complete denitrification gene clusters from subtidal microplastic biofilms and constructed a synthetic community (SynCom) with them. The SynCom exhibited high nitrate removal efficiency and stability under high salinity, with stable community composition. Metatranscriptomic analysis further indicated functional division of labor through differential metabolic reprogramming: P5F4–3 upregulated denitrification genes and stress response genes while downregulating energy metabolism and ribosome synthesis; PMMA7 specifically upregulated the entire nitrous oxide reductase gene cluster, focusing on the terminal denitrification step, while suppressing central carbon metabolism, nutrient uptake, and biosynthesis; pe10 enhanced ribosome biosynthesis, energy metabolism, and cofactor synthesis while simplifying nutrient uptake and motility-related pathways. In carrier colonization experiments, the SynCom formed biofilms and effectively removed nitrate without significant nitrite accumulation. Moreover, the nitrate removal efficiency was validated in a laboratory-scale moving bed biofilm reactor, supporting its potential for practical application. Collectively, these findings identify marine microplastic biofilms as a cultivable source of salt-tolerant denitrifiers and demonstrate the potential of defined SynCom for saline denitrification.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102795"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alfred Elikem Kwami Afedzi, Preuk Tangpromphan, Pitiya Kamonpatana, Pramuk Parakulsuksatid
{"title":"Surfactant-mediated enzyme availability enhances high-solids simultaneous saccharification and fermentation for bioethanol production from pretreated oil palm trunk at reduced cellulase dosage","authors":"Alfred Elikem Kwami Afedzi, Preuk Tangpromphan, Pitiya Kamonpatana, Pramuk Parakulsuksatid","doi":"10.1016/j.biteb.2026.102801","DOIUrl":"10.1016/j.biteb.2026.102801","url":null,"abstract":"<div><div>High-solids ethanol production from pretreated oil palm trunk (OPT) is constrained by non-productive cellulase adsorption and reduced biocatalyst performance, which are exacerbated by the fibrous morphology of the pretreated substrate. This study evaluated how substrate physical form and nonionic surfactants influence enzyme adsorption, enzymatic hydrolysis, and simultaneous saccharification and fermentation (SSF) of pretreated OPT. Brief milling (10 s mechanical fiber opening) improved substrate accessibility and increased glucose release compared with unmilled OPT. Enzyme availability was assessed by residual cellulase activity remaining in the liquid phase after enzyme–substrate contact. Tween 80 retained more cellulase activity in the liquid phase than Tween 20 under comparable conditions. In glucose fermentation, 0.3% (<em>w</em>/<em>v</em>) Tween 80 increased ethanol to 24.38 ± 0.69 g/L compared with 21.15 ± 1.20 g/L in the control, while 0.5% (<em>w</em>/<em>v</em>) Tween 20 reduced ethanol to 18.78 ± 0.42 g/L and decreased cell concentration. In batch SSF at 5% (w/v) solids, 0.3% (<em>w</em>/<em>v</em>) Tween 80 produced 18.09 ± 0.29 g/L ethanol at 13 FPU/g and reached maximum ethanol at 60 h. In high-solids fed-batch SSF at 23% (<em>w</em>/<em>v</em>) final solids, 0.3% (w/v) Tween 80 produced 64.21 ± 1.14 g/L ethanol at 13 FPU/g and reached maximum ethanol at 72 h, which was 12 h earlier than the control conditions. By avoiding extended milling and sieving, this study demonstrates that brief milling with optimized Tween 80 dosing can reduce cellulase input while sustaining ethanol performance in batch and high-solids fed-batch SSF.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102801"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of operating conditions on carbamazepine removal in a simultaneous nitrification–denitrification sequencing batch reactor","authors":"Susan N. James, Arya Vijayanandan","doi":"10.1016/j.biteb.2026.102786","DOIUrl":"10.1016/j.biteb.2026.102786","url":null,"abstract":"<div><div>Dynamic redox conditions in simultaneous nitrification-denitrification (SND) systems have been shown to enhance pharmaceutical degradation in wastewater treatment. The present study investigated the fate, kinetics, and transformation of the emerging contaminant carbamazepine (CBZ) in a sequencing batch reactor (SBR) under different operating conditions. A varying aeration pattern resulted in stable nitrogen removal and a maximum CBZ removal of 55.7 ± 0.1% at an influent concentration of 50 μg/L. Batch biodegradation studies revealed that SND-SBR microbial consortia exhibited the highest biodegradation potential (K<sub>bio</sub> = 0.17 L/g-VSS·d), exceeding those of nitrifying and denitrifying cultures. Eleven transformation products (TPs) of CBZ were identified, indicating that removal occurred primarily through oxidative and reductive biotransformation pathways. Microbial community analysis indicated that CBZ degradation was driven by the coexistence of autotrophic nitrifiers and denitrifiers, including <em>Nitrospira</em>, <em>Hyphomicrobium</em>, and members of <em>Saprospiraceae</em> and <em>Hyphomonadaceae</em>. Overall, the results demonstrated that operationally induced redox cycling in an SND-SBR promoted the synergistic microbial interactions that enhance CBZ biodegradation while maintaining effective nitrogen removal. The study highlighted the applicability of SND as a biological treatment strategy for emerging contaminants.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102786"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148477081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vallu Ramakrishna, S.G. Sahu, Deepak K. Mahato, Pinaki Sarkar, Ashish Mukharjee, Arshi Perween, Keshav Raghuvanshi, Arnold Luwang Usham, Mustaq Ansari, Sidharth Bari, R.E. Masto, Pradip K. Banerjee, Dhaneshwar Mahto
{"title":"A comparative study of thermal and mechanical properties of binder-free castor stalk pellets produced via pre- and post-torrefaction routes","authors":"Vallu Ramakrishna, S.G. Sahu, Deepak K. Mahato, Pinaki Sarkar, Ashish Mukharjee, Arshi Perween, Keshav Raghuvanshi, Arnold Luwang Usham, Mustaq Ansari, Sidharth Bari, R.E. Masto, Pradip K. Banerjee, Dhaneshwar Mahto","doi":"10.1016/j.biteb.2026.102764","DOIUrl":"10.1016/j.biteb.2026.102764","url":null,"abstract":"<div><div>This study investigates the thermal, mechanical, moisture-absorption, and energy balance characteristics of torrefied castor stalk (TCS) pellets made through two different processing methods. In route-1, biomass was first torrefied at elevated temperatures (250 °C, 275 °C, and 300 °C) and then pelletized (pre-TCS). In route-2, pellets were prepared from raw biomass and then torrefied (post-TCS) at various temperatures (250 °C, 275 °C, and 300 °C). At 300 °C, the post-TCS pellets showed a higher heating value (23.80 ± 0.08 MJ/kg) compared to pre-TCS (21.96 ± 0.165 MJ/kg) and CS-raw pellets (18.80 ± 0.22 MJ/kg). Multi-linear regression analysis using SPSS version 28 revealed that impact of moisture, lignin, and extractive contents on the mechanical properties of TCS pellets. The post-TCS pellets absorbed less moisture than the pre-TCS pellets, resulting in only a slight decrease in HHV of 1.65 MJ/kg, even after 144 h of exposure to open laboratory conditions. The thermal stability was evaluated by STA/DSC, and FE-SEM confirmed a fused surface morphology, which increased the mechanical strength of post-TCS-300 pellets. The post-TCS-300 pellets demonstrated a higher energy balance of 60.97 MJ/kg compared to pre-TCS-300 pellets (55.51 MJ/kg). Overall, route-2 showed improved fuel qualities, including higher heating value, energy yield, mass yield, energy density, moisture-absorption resistance, thermal stability, durability, and O/C & H/C ratios. These results suggest that the post-torrefied pelletization process in route-2 is a more valuable option, suitable for both domestic and industrial uses.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"34 ","pages":"Article 102764"},"PeriodicalIF":5.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148499285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}