Bioresource Technology Reports最新文献

{"title":"Remediation of ciprofloxacin in water using biofilters inoculated with Pleurotus ostreatus and Pleurotus djamor","authors":"A.J. Alvillo-Rivera,&nbsp;C.H. Mateo-Calvillo,&nbsp;M.F. Gutiérrez-Jahén,&nbsp;L.G. Treviño-Quintanilla,&nbsp;R.A. Guillén-Garcés,&nbsp;I.E. Villegas-Mendoza","doi":"10.1016/j.biteb.2026.102976","DOIUrl":"10.1016/j.biteb.2026.102976","url":null,"abstract":"<div><div>The presence of emerging contaminants in water bodies, especially pharmaceuticals like ciprofloxacin, represents a growing challenge due to their high persistence in the environment and their limited ability to be removed by conventional water treatment systems. The main objective of this study was to evaluate the efficiency of biofilters inoculated with the basidiomycete fungi <em>Pleurotus ostreatus</em> and <em>Pleurotus djamor</em> for the removal of ciprofloxacin from synthetic water. Three different substrates were used: 1) polyurethane cubes, 2) Kaldnes K1, and 3) corncobs, to determine which resulted in the best fungal growth and retention, as well as the least interaction with ciprofloxacin.</div><div>The results showed that the fungi exhibited greater growth in less time when using polyurethane cubes as a support medium, as well as better retention of the contaminant. Furthermore, when evaluating ciprofloxacin sorption on the supports, polyurethane cubes were determined to be the most viable option due to their low contaminant sorption capacity (&lt;10%), followed by Kaldnes K1 and lastly corncob, while both fungi (<em>P. ostreatus</em> and <em>P. djamor</em>) can retain approximately 40% of the contaminant through sorption.</div><div>Operating biofilters packed with polyurethane cubes and inoculated with <em>P. ostreatus</em> and <em>P. djamor</em> achieved overall removal efficiencies of 98% and 95%, respectively. The contaminant removal was attributed to a combination of sorption on the support (9%), sorption by the fungi (40%), and biodegradation by <em>P. ostreatus</em> (49%) and <em>P. djamor</em> (49%), demonstrating that this type of technology is a viable and sustainable alternative for removing emerging contaminants from water.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102976"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pilot-scale production of microbial protein and PHA using purple phototrophic bacteria for single-cell bioplastics","authors":"Luis D. Allegue,&nbsp;Sergio J. Gonzalez Camara,&nbsp;Maarten Muys,&nbsp;Shreyash Anil Gujar,&nbsp;Steven De Meester,&nbsp;Leen Bastiaens,&nbsp;Queenie Simons,&nbsp;Heleen De Wever,&nbsp;Joost Brancart,&nbsp;Siegfried E. Vlaeminck","doi":"10.1016/j.biteb.2026.102968","DOIUrl":"10.1016/j.biteb.2026.102968","url":null,"abstract":"<div><div>The shift from fossil-based plastics to sustainable alternatives requires biodegradable biopolymers produced from renewable resources. Here, we report a pilot-scale (220<em>L</em>) production of phototrophic purple bacteria (PPB) biomass rich in microbial protein and polyhydroxybutyrate (PHB), grown on ethanol as the sole organic carbon source. The system operated stably for 13 weeks with a synthetic co-culture of <em>Cereibacter sphaeroides</em> and <em>Rhodopseudomonas palustris</em>, reaching biomass productivities of 2.15 ± 0.12 g VSS L⁻¹ d⁻¹. The biomass accumulated 86% biopolymers: 42% PHB, 44% protein. A mild aqueous extraction yielded protein- and PHB-enriched fractions, both amenable to glycerol-based plasticization, enabling the first PPB-derived single-cell bioplastic films. The results show that solvent-intensive PHB purification might be avoided when single-cell disrupted biomass matrices are used, simplifying downstream processing. This pilot-scale integrated approach is compatible with carbon-recycled ethanol as a future feedstock, supporting PPB as a robust platform for producing biodegradable single-cell bioplastic film materials for a circular bioeconomy.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102968"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-based preservation of raw goatskin using Polyalthia longifolia: Process monitoring, leather quality assessment, and wastewater characterization","authors":"Md Abul Hashem,&nbsp;Md Shahriar Shahadat,&nbsp;Jannatul Nime Tabassum,&nbsp;Md Mukimujjaman Miem,&nbsp;Abid Hassan,&nbsp;Md Enamul Hasan Zahin,&nbsp;Sangjukta Mreedha,&nbsp;Modinatul Maoya","doi":"10.1016/j.biteb.2026.103001","DOIUrl":"10.1016/j.biteb.2026.103001","url":null,"abstract":"<div><div>Preservation of raw hide/skin is crucial and the primary step to prevent microbial degradation. The most commonly practiced wet-salting method globally is to apply sodium chloride (NaCl) to flesh side of raw hide/skin. It generates a large pollution load, especially salinity and total dissolved solids (TDS) in the emitted wastewater. The literature has shown promising solutions to overcome this problem using phyto-based preserving agents, but has failed to describe the mechanisms and effects on the collagen bundles, which discouraged their use in industrial applications. In this investigation, ground leaf of <em>Polyalthia longifolia</em> was introduced as a promising alternative curing agent to minimize the use of NaCl. A preliminary experiment revealed optimum preservation lever: 6% ground leaf + 4% salt. The final experiment is conducted for 22 days, considering hair slip, hydrothermal stability, odor, moisture content, nitrogen content, and microbial count to assess the performance of the proposed preserving agents. Potential mechanisms and effects on collagen were described using FTIR analysis, alongside cost analysis, to support mass-scale use. The physicomechanical properties – tensile strength, ball burst load, and elongation at break – were evaluated for both conventional and experimental manufactured leather that satisfied the standard limit. Reductions of biochemical oxygen demand (BOD₅), chemical oxygen demand (COD), total dissolved solids (TDS), chloride content, and electrical conductivity (EC) were 65.13%, 76.42%, 70.86%, 59.49%, and 12.81%, respectively, compared to conventional soaking wastewater. SEM was used to observe the compact, intact collagen fiber on the surface. The study demonstrates an innovative, eco-friendly preservation method for the green leather industry.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 103001"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental optimization of biological methanation with Methanothermobacter thermoautotrophicus ΔH in a lab-scale trickle-bed reactor","authors":"Lucas Schindhelm,&nbsp;Katharina Herkendell","doi":"10.1016/j.biteb.2026.102964","DOIUrl":"10.1016/j.biteb.2026.102964","url":null,"abstract":"<div><div>The European Commission’s REPowerEU plan targets a tenfold increase in biomethane production by 2030, yet German production cannot scale proportionally due to limited biogenic feedstock. Biological methanation (BM) offers an alternative methanation pathway. We operate a <span><math><mrow><mn>0.005</mn><mspace></mspace><msup><mrow><mi>m</mi></mrow><mrow><mn>3</mn></mrow></msup></mrow></math></span> trickle-bed reactor (TBR) at <span><math><mrow><msub><mrow><mi>p</mi></mrow><mrow><mi>op</mi></mrow></msub><mo>=</mo><mn>0.25</mn><mspace></mspace><msub><mrow><mi>MPa</mi></mrow><mrow><mi>abs</mi></mrow></msub></mrow></math></span> with <em>Methanothermobacter thermoautotrophicus</em> <span><math><mrow><mi>Δ</mi><mspace></mspace><mi>H</mi></mrow></math></span>. By shifting the flow regime from bubble to trickle flow the methane purity <span><math><msub><mrow><mi>y</mi></mrow><mrow><msub><mrow><mi>CH</mi></mrow><mrow><mn>4</mn></mrow></msub></mrow></msub></math></span> instantaneously increases from 84.8% to 91.8% (all <span><math><mrow><mo>±</mo><mspace></mspace><mn>2</mn><mtext>%</mtext></mrow></math></span>) at a gas-hourly space velocity of <span><math><mrow><mn>1.2</mn><mspace></mspace><msup><mrow><mi>h</mi></mrow><mrow><mo>−</mo><mn>1</mn></mrow></msup></mrow></math></span>. Switching from counter-current to co-current operation further elevates <span><math><msub><mrow><mi>y</mi></mrow><mrow><msub><mrow><mi>CH</mi></mrow><mrow><mn>4</mn></mrow></msub></mrow></msub></math></span> to a peak purity of <span><math><mrow><mn>98</mn><mo>.</mo><msubsup><mrow><mn>7</mn></mrow><mrow><mo>−</mo><mn>2.0</mn></mrow><mrow><mo>+</mo><mn>1.3</mn></mrow></msubsup><mtext>%</mtext></mrow></math></span>. We identify that in co-current operation the gas flow more closely resembles ideal plug-flow, whereas counter-current flow aggravates axial dispersion. A maximum daily methane production rate of <span><math><mrow><mi>M</mi><mi>P</mi><msub><mrow><mi>R</mi></mrow><mrow><mi>R</mi><mo>,</mo><mi>max</mi></mrow></msub><mo>=</mo><mn>0.36</mn><mo>±</mo><mn>0.01</mn><mspace></mspace><msup><mrow><mi>h</mi></mrow><mrow><mo>−</mo><mn>1</mn></mrow></msup></mrow></math></span> at <span><math><mrow><msub><mrow><mi>y</mi></mrow><mrow><msub><mrow><mi>CH</mi></mrow><mrow><mn>4</mn></mrow></msub></mrow></msub><mo>=</mo><mn>94.8</mn><mtext>%</mtext></mrow></math></span> was achieved. The results show the optimization potential to support upscaling of BM.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102964"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic pretreatment of sugarcane bagasse with engineered Trichoderma reesei enzymes: Biohydrogen potential and life cycle assessment","authors":"Camila Abreu B. Silva Rabelo,&nbsp;Giovana Golin Nicolino,&nbsp;Giovanna Lovato,&nbsp;Túlio Queijo de Lima,&nbsp;Mario T. Murakami,&nbsp;Maria Bernadete Amâncio Varesche","doi":"10.1016/j.biteb.2026.102985","DOIUrl":"10.1016/j.biteb.2026.102985","url":null,"abstract":"<div><div>Sugarcane bagasse (SCB) is an abundant agro-industrial byproduct, with strong potential for renewable hydrogen (bioH₂) production. This study reports, for the first time, the application of a β-glucosidase-rich enzyme cocktail produced by engineered <em>Trichoderma reesei</em> RUT-C30 as a pretreatment strategy for SCB valorization. Process optimization identified an enzyme loading of 155 mg-protein/g-SCB and a hydrolysis time of 32 h of hydrolysis, yielding 16.0 g/L of soluble sugars. Subsequent dark fermentation by <em>Clostridium butyricum</em> achieved 282.5 mmol-H₂/L, among the highest values reported for SCB-based systems. Structural analyses revealed significant delignification (34.5%) and extensive solubilization of cellulose (53.6%) and hemicellulose (62.1%), thereby enhancing carbohydrate accessibility without harsh chemicals. A life cycle assessment comparing six biohydrogen routes showed that the enzymatic pretreatment exhibited the lowest carbon footprint (40 kg-CO₂eq/kg-H₂), driven by reduced nutrient inputs and chemical oxygen demand discharge. Overall, this approach enhances both process efficiency and environmental sustainability for lignocellulosic bioH₂ production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102985"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"White-rot fungal bioaugmentation alters DOM evolution and reduces the operational mobility of Cu, Pb, and Cr during sewage sludge composting","authors":"Meihua Zhao,&nbsp;Hongyuan Lei,&nbsp;Shangchun Chen,&nbsp;Zhen Yu,&nbsp;François Nkinahamira","doi":"10.1016/j.biteb.2026.102984","DOIUrl":"10.1016/j.biteb.2026.102984","url":null,"abstract":"<div><div>Heavy-metal mobility is a major constraint on the safe land application of sewage sludge compost, as environmental risk depends more on labile fractions than on total concentrations. This study evaluated the effects of white-rot fungal bioaugmentation (<em>Phanerochaete chrysosporium</em>) on the evolution of dissolved organic matter (DOM) and the operational mobility of Cu, Pb, and Cr during sewage sludge composting, using an uninoculated control (CK) for comparison. DOM was characterized using UV–visible indices and excitation-emission matrix fluorescence coupled with PARAFAC analysis, and metal partitioning was assessed using water-soluble extraction and modified BCR sequential extraction. Across composting, DOM in both treatments became more humified, with stronger shifts in the bioaugmented system. PARAFAC analysis indicated declines in protein-like fluorescence and relative increases in humic-associated components under the bioaugmentation strategy. Concurrently, bioaugmentation significantly reduced the terminal operationally mobile fraction (F1 + F2 + F3) of Cu (1.73% vs. CK: 2.43%, <em>p</em> &lt; 0.01) and altered the overall passivation trajectory of Cr (ANOVA, <em>p</em> &lt; 0.001, reducing to 0.26%). For Pb, despite a non-significant overall effect (ANOVA, <em>p</em> = 0.196), bioaugmentation achieved a higher overall passivation rate (56.4% vs. CK, 51.8%). Furthermore, fluorescence analyses indicated that the enhanced DOM restructuring associated with bioaugmentation, transitioning from protein-like to humic-like components, closely tracked the reduced operational mobility of Cu, Pb, and Cr, pointing to a process-level linkage between DOM humification and metal passivation. Ultimately, fungi-assisted composting acts as an effective operational strategy, optimizing heavy-metal passivation by actively promoting continuous DOM evolution.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102984"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of biogas yield from organic waste using pongamia pinnata seed shell as bio-additive through integrated experimental and statistical analysis","authors":"Utkarsh Baranwal,&nbsp;Shubham More,&nbsp;Ashootosh Mandpe","doi":"10.1016/j.biteb.2026.102983","DOIUrl":"10.1016/j.biteb.2026.102983","url":null,"abstract":"<div><div>The rapid increase in organic waste production, coupled with the depletion of fossil fuels, has sparked interest in developing sustainable waste-to-energy methods. Anaerobic digestion (AD) of food waste (FW) is a promising technology for producing biogas, though it can be limited by substrate inhibition and process instability. This study highlights the potential of <em>Pongamia pinnata</em> seed shell powder as an inexpensive, plant-based bio-additive to improve AD performance. Cow dung was used as an inoculum to conduct batch experiments in borosilicate reactors with a 500 mL working volume. Additive doses of 1 g, 3 g, and 5 g were tested, and FW loading was performed at 20 g, 30 g, and 40 g. Biogas production was observed continuously for 30 days, and optimization using RSM was performed using a central composite design. Biogas yield (cumulative) rose to 1445 mL at maximum loading and additive level as compared to 1022 mL (control) at the same conditions. The highest specific yield (140.1 mL/g VS) was obtained at 30 g FW with a 5 g additive, representing a 44.6% increase. The quadratic model was found to be significant by statistical analysis (F = 30.58, <em>p</em> &lt; 0.0001; R² = 0.8793). ANOVA diagnostics confirmed the adequacy and robustness of the developed quadratic model, highlighting significant nonlinear interactions between substrate loading and additive dosage. The study establishes <em>Pongamia pinnata</em> seed shell powder as a promising plant-based additive for optimized biogas production and provides a statistically validated framework for process optimization in anaerobic digestion systems.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102983"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of methods for the biosynthesis of polyhydroxyalkanoates using shea butter as a carbon source by Cupriavidus necator Re2058/pCB113","authors":"Comfort Olukemi Bamigboye,&nbsp;Kumar Sudesh","doi":"10.1016/j.biteb.2026.102971","DOIUrl":"10.1016/j.biteb.2026.102971","url":null,"abstract":"<div><div>Polyhydroxyalkanoates (PHAs) are biodegradable polyesters produced by some bacteria and archaea as intracellular carbon and energy reserves. This study investigated the use of shea butter (SB), an underutilized lipid-rich agricultural product, as a carbon source for PHA biosynthesis by <em>Cupriavidus necator</em> Re2058/pCB113. Different emulsifiers (Tween 80 and gum arabic) and substrate combinations (shea butter, palm olein, waste cooking oil) were carefully controlled to enhance PHA yield. Shake-flask and bioreactor experiments were conducted under nitrogen-limited conditions. Emulsifying 10 g/L shea butter with Tween 80, yielded 6.6 ± 0.8 g/L CDW, 66.5 ± 7.6 wt% of PHA content, and 4.4 g/L PHA concentration. In a 5 L bioreactor, the highest yield was achieved at 40 h with 7.5 ± 0.5 g/L CDW and 78.7 ± 1.4% PHA content, comprising 89.9 mol% 3-hydroxybutyrate (3HB) and 10.1 mol% poly 3-hydroxybutyrate co-3-hydroxyhexanoate copolymer. Further emulsification strategy enhanced biomass yield, producing 10 g/L CDW and 9.7 ± 0.3 g/L PHA. The results demonstrate the feasibility of using unrefined shea butter as an inexpensive lipid substrate for PHA production, contributing to sustainable biopolymer development.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102971"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and crystal evolution of the lignocellulosic components of coconut fibers during the alkaline and ultrasound treatments","authors":"Mayky André Olivos-Ramírez,&nbsp;Pablo González-Morones,&nbsp;Israel Sifuentes-Nieves,&nbsp;Zureima García-Hernández,&nbsp;Pamela Celeste Flores-Silva,&nbsp;Alejandro Garza-Santibañez,&nbsp;Ernesto Hernández-Hernández,&nbsp;José Francisco Hernández-Gámez","doi":"10.1016/j.biteb.2026.102972","DOIUrl":"10.1016/j.biteb.2026.102972","url":null,"abstract":"<div><div>In this study, we demonstrate that a combined alkaline/ultrasonic treatment is highly effective in modifying the chemical composition, crystallinity, and morphology of coconut fibers (CCFs). The dual treatment primarily extracts surface hemicellulose while preserving the internal hemicellulose; notably, the lignin content remains largely unaffected. Furthermore, silicon microparticles and other inorganic components are almost completely eliminated during the dual treatment. SEM, TGA, and FTIR analyses reveal that alkaline treatment is more influential than ultrasonic treatment, though their combination yields a synergistic effect. After 15 min of dual treatment, the crystallinity index (CI) of the treated CCFs increased significantly to 74%, compared to 58% for the pristine fibers as shown in the XRD analysis. However, extended sonication decreased CI, a phenomenon attributed to localized erosion in the crystal regions induced by acoustic cavitation. Consequently, the water retention capacity of the fibers decreased from 68 to 48 wt%. Solid-state ¹³C NMR further corroborated the removal of hemicellulose and the partial extraction of lignin. This study demonstrates the effectiveness of dual alkaline/ultrasound treatment to control the properties of coconut fibers, which can be used as reinforcement in polymer matrices.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 102972"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148935027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted alkaline extraction of pearl millet protein: Characterization of physical, structural, thermal, and techno-functional properties","authors":"Upendra Singh,&nbsp;Raj Kumar Duary,&nbsp;Sunil Meena,&nbsp;Kappat Valiyapeediyekkal Sunooj,&nbsp;Kamalesh Kumar Meena,&nbsp;Chetan Prakash Yadav","doi":"10.1016/j.biteb.2026.103011","DOIUrl":"10.1016/j.biteb.2026.103011","url":null,"abstract":"<div><div>Millets are gaining attention as nutritional cereal alternatives due to their functional and therapeutic properties, partly attributed to bioactive peptides in their native proteins. This study investigated the structural, functional, and thermal properties of pearl millet proteins (PMPs) treated with ultrasound (US) to enhance their food application potential. PMPs were isolated via acid-base precipitation from defatted flour, followed by US treatment (5, 10, and 15 min at 45 kHz). The control sample exhibited the largest particle size (1321 nm), whereas sample treated with US for 10 and 15 min showed significantly reduction particle size (789.30 nm). Additionally, the zeta potential shows increasing trends shifting from −15.20 mV in the control sample to −23.96 mV in the US10 treatment, which collectively indicates notable structural changes in the protein. FTIR and circular dichroism analyses revealed modifications in secondary protein structures, including α-helix and β-sheet content. XRD confirmed the presence of these structures, while SEM showed more uniform and compact protein fragments in US-treated samples. Thermal analysis highlighted degradation behavior, and NMR confirmed amino and organic acid presence. US treatment thus significantly enhanced the functional properties of PMPs, improving their potential for food applications.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"35 ","pages":"Article 103011"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148935028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}