Bioresource Technology Reports最新文献

{"title":"Impact of the rotational speed and counter electrode configuration on the performance of a rotating disc bioelectrochemical reactor (RDBER) operated as microbial electrolysis cell","authors":"Zhizhao Xiao ,&nbsp;Max Rümenapf ,&nbsp;Max Hackbarth ,&nbsp;Andrea Hille-Reichel ,&nbsp;Harald Horn ,&nbsp;Johannes Eberhard Reiner","doi":"10.1016/j.biteb.2025.102208","DOIUrl":"10.1016/j.biteb.2025.102208","url":null,"abstract":"<div><div>A 10 L Rotating Disc Bioelectrochemical Reactor (RDBER) was operated as a microbial electrolysis cell (MEC) under different rotational speeds and counter electrode configurations. Increasing the anode's speed from 0.25 to 1 rpm raised the anodic current density from 0.55 ± 0.14 to 1.00 ± 0.07 A m⁻² while increasing hydrogen production rates from 0.05 ± 0.01 to 0.18 ± 0.01 L_H₂ L_R⁻¹ d⁻¹. Higher speeds provided no further benefit. Moving the counter electrodes to the upper reactor half reduced observed hydrogen shuttling. The modified RDBER reached current densities of 1.98 ± 0.11 A m⁻² and 0.99 ± 0.03 L_H₂ L_R⁻¹ d⁻¹ hydrogen production. Optical coherence tomography confirmed biofilm morphology changes but no significant increase in biovolume or substratum coverage. Hydrogen recovery remained below 50 %. While the RDBER achieved high volumetric current densities and volumetric hydrogen production rates compared to other MEC pilots, improvements in anodic current density and cathodic hydrogen recovery are required for practical application.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102208"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing biogas production efficiency through physical, chemical, thermal, and hybrid pre-treatment of olive mill wastewater","authors":"Hasan Ateş ,&nbsp;Fadime Taner","doi":"10.1016/j.biteb.2025.102206","DOIUrl":"10.1016/j.biteb.2025.102206","url":null,"abstract":"<div><div>In this study, various pre-treatment methods were applied to enhance the biogas production efficiency of olive mill wastewater (OMW) collected from the decanter outlet of an olive oil mill operating with a three-phase system. The effects of ultrasonic (US), chemical (HNO₃), microwave (MW), and their combinations on the anaerobic digestion of OMW were evaluated. While HNO₃ pre-treatment alone and in combination with other methods improved biogas production efficiency, MW pre-treatment alone significantly reduced biogas yield. The highest volumetric cumulative biogas production of 231.5 mL (1.78 mL Biogas/gVSd) was achieved in the hybrid HN20 + US10 pre-treated sample, where HNO₃ was applied at 20 % of the total solids in raw OMW, followed by US pre-treatment for 10 min. The total phenol and chemical oxygen demand (COD) removal percentages for the HN20 + US10 pre-treated sample were determined as 25.9 % and 77.2 %, respectively.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102206"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrafiltration for the assessment of supernatants and biomass from Spirulina cultures supplemented with L-tryptophan","authors":"Hugo Fabián Lobatón ,&nbsp;Nataly López Mejía ,&nbsp;Claudia Salazar-González","doi":"10.1016/j.biteb.2025.102214","DOIUrl":"10.1016/j.biteb.2025.102214","url":null,"abstract":"<div><div><em>Spirulina</em>, a cyanobacterium renowned for its nutritional value, has recently gained attention as an agricultural biostimulant due to its plant growth-promoting properties. On the fifth day of cultivation, L-tryptophan (1.0 g/L) -a precursor to phytohormones like indole-3-acetic acid (IAA)-was supplemented, leading to the production of 100 mg/L of IAA and 3 mg/L of total carotenoids in the supernatant after 100 h. Ultrafiltration concentrated these molecules, achieving recoveries of 33 % for IAA and 31 % for carotenoids, along with a 12-fold concentration factor. The lyophilized retentate, rich in IAA and carotenoids, was applied to mung bean plants, where an application of 0.2 mg/L of IAA increased secondary root length by 18 % and the number of roots by 35 %. Additionally, the biomass was processed to produce a powder rich in phycocyanin, chlorophyll <em>a</em>, carotenoids, and proteins, making it suitable for cosmetic formulations. This dual approach enables the sustainable utilization of <em>Spirulina</em>, using the biomass for cosmetics and the supernatant in agriculture, with ultrafiltration effectively reducing water content and preserving active compounds.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102214"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dark period and reactor thickness on Arthrospira maxima continuous cultivation under high frequency pulsed light","authors":"Adriano Porcelli ,&nbsp;Lisa Borella ,&nbsp;Nicola Trivellin ,&nbsp;Eleonora Sforza","doi":"10.1016/j.biteb.2025.102210","DOIUrl":"10.1016/j.biteb.2025.102210","url":null,"abstract":"<div><div>High-frequency pulsed-light is interesting to improve the efficiency of artificially-illuminated reactors. Even though it is demonstrated that certain pulsed conditions can increase productivity, the flashing-light effect strongly depends on the operative conditions. In this study, <em>Arthrospira maxima</em> was cultivated under pulsed-light, focusing in particular on the effect of dark time duration and reactor thickness, to find the best parameters for a more efficient process. Being the nature of pulsed light complex in terms of number of interdependent parameters, a novel respirometric protocol was developed to obtain a quick response of the effect of pulsed light on photosynthetic efficiency. The protocol was then validated by continuous cultivation experiments at steady state. Results confirmed that a light period between 50 μs and 100 μs ensure the highest Oxygen Production Rate, while a minimum dark time of about 5 ms is necessary to maximize photosynthetic efficiency. Increasing the dark periods does not improve biomass productivity. The light pulse intensity plays also a role, with values above 17,850 μmol m⁻² s⁻¹ causing photoinhibition. Interestingly, the effect of pulsed light is also strongly linked to the thickness of the photobioreactor: at increased light paths, pulsed-light allows better photosynthetic efficiency and biomass concentration compared to continuous light, thanks to its higher penetration. The light regime parameters, thought, need to be set properly to avoid photoinhibition. In summary, this study allows a deeper understanding of the best light parameters for continuous cultivations exploiting pulsed light technology and provides a foundation for the comprehension of the flashing light effect, particularly focusing on the effect of dark time.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102210"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-fermentation of sugar-alcohol industry waste to produce hydrogen and value-added metabolites","authors":"Bruna S. Dionizio ,&nbsp;Camila A.B.S. Rabelo ,&nbsp;Caroline V. Rodrigues ,&nbsp;Franciele P. Camargo ,&nbsp;Edson L. Silva ,&nbsp;Dulce H.F. de Souza ,&nbsp;Maria Bernadete A. Varesche","doi":"10.1016/j.biteb.2025.102211","DOIUrl":"10.1016/j.biteb.2025.102211","url":null,"abstract":"<div><div>The research focused on optimizing hydrogen production through the co-fermentation of three sugar-alcohol industry residues, sugarcane bagasse (SCB), filter cake (FC), and sugarcane vinasse (SCV). Batch assays were performed using enzymatically pretreated SCB (23.4 g·L⁻¹) combined with varying concentrations of FC (7.9–37.7 g·L⁻¹) and SCV (8.3–31.7 g COD·L⁻¹). A mixed microbial culture bioaugmented with <em>Clostridium butyricum</em> was employed as the inoculum. In the process optimization the highest hydrogen yield (3239.78 mL·L⁻¹) was achieved under conditions of 33.1 g·L⁻¹ FC and 28.3 g COD·L⁻¹ SCV, along with the production of 7132.8 mg·L⁻¹ of valuable organic acids and solvents. Additionally, SCV at higher concentrations (31.7 g COD·L⁻¹) favored homoacetogenesis, leading to an acetic acid accumulation of 7526.8 mg·L⁻¹. Microbial community analysis by 16S rRNA sequencing revealed the dominance of <em>Clostridium</em>, <em>Caproiciproducens</em>, <em>Anaerotruncus</em>, <em>Cellulosilyticum</em>, and members of Lachnospiraceae, whose inferred functional genes included those encoding endoglucanase, xylanase, arabinofuranosidase, and lignin-degrading peroxidases. The findings highlight the potential of integrated bioconversion of sugar-alcohol industry residues to improve hydrogen and bioproduct generation, supporting more sustainable waste management and renewable energy production.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102211"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic and antagonistic interactions in the co-pyrolysis of macadamia nutshells and coffee husks: Thermogravimetric, kinetic, and thermodynamic insights","authors":"Ocident Bongomin ,&nbsp;Sherien Elagroudy ,&nbsp;Josphat Igadwa Mwasiagi ,&nbsp;Charles Nzila","doi":"10.1016/j.biteb.2025.102207","DOIUrl":"10.1016/j.biteb.2025.102207","url":null,"abstract":"<div><div>The co-pyrolysis of lignocellulosic residues presents a promising pathway for sustainable bioenergy production and waste management. However, the underlying synergistic and antagonistic interactions during co-pyrolysis remain inadequately explored. This study investigates the thermal behavior of macadamia nutshells (MS) and coffee husks (CH) blends (CH75MS25, CH50MS50, and CH25MS75) using thermogravimetric analysis (TGA) under an inert nitrogen atmosphere. The reaction mechanism was evaluated using the Criado's master plot, while activation energy (<em>E</em>_<em>a</em>) and frequency factor (<em>A</em>) were determined via the correlation method and Kissinger method, respectively. Results revealed notable synergistic interactions in the CH50MS50 blend, marked by a 3.8 % reduction in <em>E</em>_<em>a</em>, indicating improved thermal reactivity. This was accompanied by a moderate reduction in <em>E</em>_<em>a</em> (186.94 kJ/mol) compared to MS (191.63 kJ/mol) and CH (183.14 kJ/mol). In contrast, the CH25MS75 blend exhibited antagonistic effects, demonstrated by a 1.96 % deviation and increased energy barriers, attributed to the dominance of lignin-rich MS suppressing volatile release. The <em>A</em> varied from 1.79E+14 min⁻¹ (CH) to 6.21E+14 min⁻¹ (MS), reflecting the inherent thermal stability of each material. Master plot analysis showed that diffusion and nucleation mechanisms (D1, P2) dominated at low conversions, while reaction-order models (F2, F3) prevailed at higher stages. Thermodynamic analysis confirmed the endothermic and non-spontaneous nature of co-pyrolysis, with enthalpy (ΔH) values around 180–190 kJ/mol, entropy (ΔS) up to 0.066 kJ/mol·K, and high Gibbs-free energy (ΔG) values exceeding 150 kJ/mol, indicating notable energy demands and limited spontaneity. These findings provide valuable insights for optimizing biomass blending strategies and advancing co-pyrolysis technologies for efficient bioenergy recovery.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102207"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination and quantification of microplastics in goat farms","authors":"Ahmad Wahyudi ,&nbsp;Roy Hendroko Setyobudi ,&nbsp;Iswahyudi Iswahyudi ,&nbsp;Marchel Putra Garfansa ,&nbsp;Evika Sandi Savitri ,&nbsp;Bayu Agung Prahardika ,&nbsp;Patmawati Patmawati ,&nbsp;Rika Diananing Putri ,&nbsp;Dodi Sukma R.A. ,&nbsp;Nico Syah Putra ,&nbsp;Emi Yunita ,&nbsp;Satriyo Krido Wahono","doi":"10.1016/j.biteb.2025.102199","DOIUrl":"10.1016/j.biteb.2025.102199","url":null,"abstract":"<div><div>Microplastics have emerged as a significant environmental threat, yet their distribution in goat farms and their impact on livestock products remain underexplored. This study aims to identify and quantify microplastics in various components of goat farming, including soil (grass fields), main feed, supplementary feed, drinking water, goat feces, and goat milk. The research methodology involved sampling 1 kg from each livestock component, followed by chemical digestion for sample preparation and microplastic analysis using FTIR spectroscopy. The microplastic data were analyzed by shape (fiber, fragment, film, filament, granule, sheet) and color (black, blue, red, transparent, yellow), as well as the polymer type, to assess their distribution across the farm. The results showed that fiber was the most dominant form of microplastic, with the highest number found in goat farm (250 particles/kg), while black dominated the entire sample and the type of polyethylene and low-density polyethylene polymer found. These findings suggest that feed, water, and the farm environment are major sources of microplastics, which may accumulate in goats' bodies and subsequently enter dairy products. This study provides valuable insights into the potential risks microplastics pose to livestock health and food safety, highlighting the need for efforts to reduce microplastic pollution in the livestock industry.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102199"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of fluid dynamic characteristics near the surface of the anode electrode on the performance of recirculation-type microbial fuel cells using computational fluid dynamics","authors":"Dale Mark N. Bristol ,&nbsp;Jian-Ting Pung ,&nbsp;Chin-Tsan Wang","doi":"10.1016/j.biteb.2025.102213","DOIUrl":"10.1016/j.biteb.2025.102213","url":null,"abstract":"<div><div>This study examines how fluid dynamic properties near the anode electrode surface (carbon cloth and carbon paper) affect the operation of recirculation-type microbial fuel cells (MFCs) at different flow rates (140, 240, and 340 mL/min) using computational fluid dynamics (CFD). The contribution of this research clarifies how changes in velocity, pressure, and shear stress influence substrate distribution and biofilm formation in the anode by modeling intricate flow patterns and mass transport phenomena at the fluid-anode microscale interface. Aside from the use of CFD simulation, the experiment was carried out by means of particle image velocimetry application accessed in the MATLAB software for the actual flow field visualization and electrochemical measurements for the voltage, current density, power density and impedance. From the statistical analysis using ANOVA, results showed that there is a significant difference in the mean power densities from flowrate + anode electrode combinations (140CC, 140 CP, 240 CC, 240 CP, 340 CC and 340 CP) and this was further confirmed using the Tukey's pairwise comparison post hoc analysis showing 140CP indeed has the highest power density at 297.60 mW/m² with a corresponding predicted boundary layer thickness from CFD at 15.7 mm.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102213"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in microalgae harvesting and dewatering: A techno-economic evaluation perspective","authors":"Durgadevi Selvaraj ,&nbsp;R. Sanjiv ,&nbsp;S. Shakira Banu ,&nbsp;M. Arivazhagan","doi":"10.1016/j.biteb.2025.102201","DOIUrl":"10.1016/j.biteb.2025.102201","url":null,"abstract":"<div><div>Commercial-scale microalgal production faces significant challenges, particularly in harvesting and dewatering, which account for 20–30 % of production costs. The energy-intensive nature of dewatering and the diverse properties of microalgal species further required cost-effective harvesting methods. This review examines recent advances in microalgal harvesting and recovery, evaluating emerging hybrid systems that address the critical limitations of traditional techniques while emphasizing their role in resource recovery and energy efficiency. Furthermore, this study presents a novel techno-economic perspective by integrating harvesting technologies with an overview of their environmental and economic impacts. These approaches distinguish this work from previous reviews by focusing on microalgae harvesting methods' practical scalability and commercial feasibility. This review bridges the gap between laboratory research and large-scale industrial applications by providing detailed insights into energy consumption, processing costs, and resource retrieval. This study contributes to the advancement of sustainable bio-economy development by providing actionable strategies.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102201"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red seaweed-based bioprocesses with Corynebacterium glutamicum","authors":"Luciana Fernandes Brito ,&nbsp;Monica G. Frøystad ,&nbsp;Anna Haug Vandenhove,&nbsp;Trygve Brautaset,&nbsp;Fernando Pérez-García","doi":"10.1016/j.biteb.2025.102203","DOIUrl":"10.1016/j.biteb.2025.102203","url":null,"abstract":"<div><div>The rising population boosts food and energy demand, requiring sustainable solutions. Red seaweed, rich in galactose-based polymers, is a promising feedstock for industrial biotechnology. To enable the industrial workhorse <em>Corynebacterium glutamicum</em> to utilize galactose, the <em>Escherichia coli</em> galactose operon <em>galETKM</em> and galactose permease gene <em>galP</em> were overexpressed as a synthetic operon. After tuning the translational initiation rate of <em>galP,</em> the engineered <em>C. glutamicum</em> strain achieved a growth rate of 0.30 ± 0.01 1/h on 1 % galactose. Further research identified native galactose uptake systems, including the phosphotransferase system components PtsG, PtsF, and the permease IolT2. Next, red seaweed hydrolysate containing galactose, xylose, and glucose was tested as carbon sources, enabling xylose use by expressing <em>xylA</em> from <em>Xanthomonas campestris</em> and endogenous <em>xylB</em>. Additionally, lysine and riboflavin production was achieved at different cultivation scales using engineered <em>C. glutamicum</em> utilizing red seaweed hydrolysate. Hence, this research pioneers red seaweed as microbial feedstock in industrial biotechnology.</div></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":"31 ","pages":"Article 102203"},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}