Biomolecules最新文献

{"title":"N-Terminal Pro-B-Type Natriuretic Peptide and Cardiac Troponin T in Stable Renal Transplant Recipients and All-Cause Mortality, Cardiovascular, and Renal Events.","authors":"Zbigniew Heleniak, Marcel G Naik, Georgios Eleftheriadis, Tomasz Madej, Fabian Halleck, Alicja Dębska-Ślizień, Klemens Budde","doi":"10.3390/biom15091298","DOIUrl":"10.3390/biom15091298","url":null,"abstract":"<p><strong>Introduction: </strong>In renal transplant recipients (RTRs), kidney graft failure and cardiovascular (CV) disease are prevalent and associated with mortality.</p><p><strong>Objectives: </strong>The objective of the study was to evaluate biomarkers, (cardiac troponin T (cTnT) and N-terminal pro-B-type natriuretic peptide (NT-proBNP)), to identify RTRs who are at greater risk of death, CV event, and graft renal survival.</p><p><strong>Patients and methods: </strong>A total of 342 stable RTRs were enrolled in this study, with a median follow-up time of 54 months. The probability of death, CV event, and renal graft survival were calculated using Kaplan-Meier analysis for the group defined by cTnT and NT-proBNP levels above the cutoff values.</p><p><strong>Results: </strong>The probability of death for troponin T level above the cut-off was 23% and for NT-proBNP 29%. For CV events the probability for troponin T was 20% and for NT-proBNP it was 21%. Troponin T concentrations above the cutoff point suggested a 25% probability of death-censored graft survival. For NT-proBNP, it was 26%. The probability of overall graft survival was 38% for patients with higher troponin T levels, and 40% for NT proBNP.</p><p><strong>Conclusions: </strong>These data suggest that cTnT and NT-proBNP could potentially identify patients at high risk for death, CV event, and graft survival.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Plasma Lipid Alterations Differentiating Psychotic and Affective Disorder Patients.","authors":"Anastasia Golubova, Elena Stekolshchikova, Anna Gareeva, Inessa Akhmerova, Ilgiz Timerbulatov, Valeria Zakurazhnaya, Daria Riabinina, Alexander Reznik, Anna Morozova, Denis Andreyuk, Georgiy Kostyuk, Daria Petrova, Anna Serkina, Philipp Khaitovich, Anna Tkachev","doi":"10.3390/biom15091296","DOIUrl":"10.3390/biom15091296","url":null,"abstract":"<p><p>Psychotic and affective disorders, including schizophrenia (SCZ) and depression (MDD), affect millions of people globally. The overlapping symptoms of these diseases and the lack of objective diagnostic tools could lead to misdiagnosis. Recent studies suggest that the analysis of plasma lipid levels may help to develop new diagnostic tools. In this study, we investigated the plasma lipidome of psychiatric patients and healthy controls to identify disease-specific lipid species. Using untargeted mass spectrometry, we profiled blood plasma lipids from 416 patients with common psychotic and affective disorders and 272 healthy individuals from two different cohorts. We observed lipidome alterations in SCZ and MDD consistent with earlier findings. In total, 144 lipids showed significant changes, with 107 of them being concordant across both disorders, and 37 being discordant. Lipids that differentiated SCZ from MDD were mainly triacylglycerols with polyunsaturated fatty acid residues decreased in MDD. In an additional group of 111 patients with bipolar, schizotypal, and schizoaffective disorders, these lipid markers suggested a trend toward separating psychotic and affective disorders. Furthermore, a logistic regression model trained on lipid data distinguished SCZ from MDD with an ROC AUC of 0.83. Taken together, these results suggest that blood lipid profiling may aid in the objective differentiation of psychotic and affective disorders.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Cardiorenal Involvement in Systemic Sclerosis Patients.","authors":"Chiara Pellicano, Giancarlo D'Ippolito, Annalisa Villa, Ottavio Martellucci, Umberto Basile, Valeria Carnazzo, Valerio Basile, Edoardo Rosato, Mariapaola Marino, Antonietta Gigante","doi":"10.3390/biom15091297","DOIUrl":"10.3390/biom15091297","url":null,"abstract":"<p><p>Systemic sclerosis (SSc) is an autoimmune disease associated with a high burden of morbidity and mortality due to organ complications. Pulmonary arterial hypertension (PAH) and cardiac involvement, characterized by chronic right ventricular (RV) pressure overload with consequent RV dysfunction and ultimately right heart failure (HF), are among these. A common comorbidity in SSc is chronic kidney disease (CKD). CKD is often present at the time of PAH diagnosis or a decline in renal function may occur during the course of the disease. CKD is strongly and independently associated with mortality in patients with PAH and HF. The cardiovascular and renal systems are closely interconnected, and disruption of this balance may result in cardiorenal syndrome (CRS). Type 2 CRS refers to CKD as a consequence of chronic HF. In clinical practice, non-specific markers such as troponin, B-type natriuretic peptide (BNP), N-terminal pro-BNP (NT-proBNP), and serum creatinine aid in CRS diagnosis. More specific biomarkers, including cystatin C (CysC), neutrophil gelatinase-associated lipocalin (NGAL), galectin-3, and soluble urokinase plasminogen activator receptor (suPAR), have shown value for diagnosis and prognosis in CRS. This study aimed to evaluate comprehensively heart/kidney damage markers related to CRS in SSc patients compared with healthy controls (HC) and to examine their association with renal and cardiac ultrasound parameters. SSc patients showed significantly higher CRS markers than HC (<i>p</i> < 0.001). SSc patients with clinically diagnosed CRS had significantly elevated galectin-3, suPAR, sNGAL, and uNGAL levels (<i>p</i> < 0.05) than SSc patients without CRS. Positive correlations were found between renal resistive index (RRI) and NT-proBNP (r = 0.335, <i>p</i> < 0.05), and between RRI and suPAR (r = 0.331, <i>p</i> < 0.05). NT-proBNP, suPAR, galectin-3, sNGAL, and uNGAL emerge as promising biomarkers for the early detection of cardiac and renal involvement in SSc patients.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Analysis of an <i>Alkalihalobacillus clausii</i> Spore-Based Probiotic Formulation Clarifies the Mechanisms Underlying Its Beneficial Properties.","authors":"Diletta Mazzantini, Marco Calvigioni, Francesco Celandroni, Alessandro Saba, Emilia Ghelardi","doi":"10.3390/biom15091294","DOIUrl":"10.3390/biom15091294","url":null,"abstract":"<p><p>Probiotics are microorganisms with recognized beneficial properties that are used to improve host health. In particular, probiotics administered as spores, such as those belonging to the genera <i>Bacillus</i> and <i>Alkalihalobacillus</i>, are attracting great interest due to their high tolerance to gastrointestinal conditions. This <i>in vitro</i> study aimed to assess the probiotic attributes potentially contributing to the <i>in vivo</i> beneficial effects of a commercial spore-based probiotic formulation composed of four <i>Alkalihalobacillus clausii</i> strains. The tolerance and survival of the spores from the formulation in simulated gastrointestinal fluids, as well as their germination rate and adhesion to mucins, were analyzed. Furthermore, metabolic properties of spore-derived vegetative cells were assessed, including lactose degradation and biosynthesis of antioxidant enzymes (catalase and superoxide dismutase), group B vitamins (B₂, B₈, B₉, and B₁₂), short-chain fatty acids (acetate, propionate, and butyrate), and D-lactate. <i>A. clausii</i> spores were shown to survive in artificial gastric juice, adhere to mucins and germinate <i>in vitro</i>, and replicate in simulated intestinal fluid, suggesting their potential resilience in the gastrointestinal tract, where they can exert beneficial effects after germination. <i>A. clausii</i> was also able to produce beneficial enzymes and metabolites, including β-galactosidase, catalase, superoxide dismutase, group B vitamins, and short-chain fatty acids, but it was unable to produce D-lactic acid. Our findings highlight the probiotic properties and potential of such <i>A. clausii</i> strains in both their spore and vegetative forms, reinforcing the clinical relevance of this multi-strain spore-based formulation for enhancing intestinal health.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Emerging Role of Mitochondrial Dysfunction in Thyroid Cancer: Mediating Tumor Progression, Drug Resistance, and Reshaping of the Immune Microenvironment.","authors":"Yating Zhang, Hengtong Han, Tingting Zhang, Tianying Zhang, Libin Ma, Ze Yang, Yongxun Zhao","doi":"10.3390/biom15091292","DOIUrl":"10.3390/biom15091292","url":null,"abstract":"<p><p>As the hub of energy metabolism and the cell's fate arbiter, mitochondria are essential for preserving cellular homeostasis and converting it from pathological states. Therefore, through mechanisms that drive metabolic reprogramming, oxidative stress, and apoptosis resistance, mitochondrial dysfunction (including mitochondrial DNA mutations, mitochondrial dynamics imbalance, mitochondrial autophagy abnormalities, mitochondrial permeability abnormalities, and metabolic disorder) can promote the progression of thyroid cancer (TC), resistance to treatment, and reshaping of the immune microenvironment. This article reviews the molecular mechanisms and characteristic manifestations of mitochondrial dysfunction in TC. It focuses on providing a summary of the main strategies currently used to target the mitochondria, such as dietary intervention and targeted medications like curcumin, as well as the clinical translational value of these medications when used in conjunction with current targeted therapies for TC and radioactive iodine (RAI) therapy in patients with advanced or RAI-refractory TC who rely on targeted therapies. The application prospects and existing challenges of emerging therapeutic methods, such as mitochondrial transplantation, are also discussed in depth, aiming to provide new perspectives for revealing the molecular mechanisms by which mitochondrial dysfunction drives the progression of TC, drug resistance, and the reshaping of its immune microenvironment, as well as providing new diagnostic and therapeutic strategies for patients with advanced or RAI-refractory TC who are reliant on targeted therapies.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translating Alzheimer's Disease Mechanisms into Therapeutic Opportunities.","authors":"Jiejia Li, Liyun Wang, Xiaodan Zhang, Jianhua Shi, Yizhun Zhu, Han Wang, Xiangyang Zhu, Qing Zhu, Jia-Lie Luo","doi":"10.3390/biom15091290","DOIUrl":"10.3390/biom15091290","url":null,"abstract":"<p><p>Alzheimer's disease (AD), characterized by progressive cognitive decline and functional impairment, is the most prevalent cause of dementia, and it poses a significant socioeconomic and caregiving burden on patients, families, and healthcare systems. Notwithstanding comprehensive research, the precise causes underlying AD remain ambiguous. Evidence increasingly indicates that AD is a multifactorial and heterogeneous disease involving a mix of genetic and environmental factors. The amyloid-cascade hypothesis, neuroinflammation and immunity, vascular pathology, and oxidative stress all fulfill significant functions in the onset and development of AD. This review primarily examines the critical pathogenesis, key biomarkers, and novel therapeutic strategies of Alzheimer's disease to inform future research directions.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of <i>Dmrt1</i> Z and W Homologs Occurred Independently in Two <i>Gekko hokouensis</i> Populations.","authors":"Momoka Senga, Nao Kaneko, Yoichi Matsuda, Kazumi Matsubara","doi":"10.3390/biom15091293","DOIUrl":"10.3390/biom15091293","url":null,"abstract":"<p><p><i>Gekko hokouensis</i> is a gecko species widely distributed across East Asia. Although most of the Japanese populations possess ZW sex chromosomes (female heterogamety), the degree of sex chromosome differentiation varies among populations. The gene encoding for Dmrt1, a transcription factor involved in testis development in vertebrates, is located on the Z and W sex chromosomes of this species and is therefore a candidate of the sex-determining gene. In this study, we investigated the gene structure of the Z and W homologs of <i>Dmrt1</i> in two populations of <i>Gekko hokouensis</i> from the Ishigaki Island and Okinawa Island. In the Ishigaki population, the ZW chromosome pair is morphologically undifferentiated, whereas in the Okinawa population the ZW pair is heteromorphic. In the Okinawa population, promoter and exon sequences were nearly identical between the Z and W homologs, and no non-synonymous substitution was detected. In contrast, the W homolog in the Ishigaki population exhibited 42 bp and 12 bp deletions in exon 2. The predicted three-dimensional protein structure revealed a rearrangement of the C-terminal region in the W homolog that may interfere with target site binding. These results indicate that differentiation between Z and W homologs of <i>Dmrt1</i> has occurred independently in each population. Our findings highlight the diversity of sex chromosome evolution and sex-determining mechanisms even within a single species.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure and Function of PML Nuclear Bodies: A Brief Overview of Key Cellular Roles.","authors":"Karolina Dorosz, Lidia Majewska, Jacek Kijowski","doi":"10.3390/biom15091291","DOIUrl":"10.3390/biom15091291","url":null,"abstract":"<p><p>Promyelocytic leukemia nuclear bodies (PML-NBs) are dynamic membrane-less organelles (MLOs) located in the nucleus that serve as regulatory hubs for multiple cellular processes. This review examines current understanding of PML-NB structure, assembly mechanisms, and their diverse functional roles. We discuss how PML-NBs interact with chromatin to influence gene expression, regulate transcription factors, and participate in protein quality control. The review highlights their critical functions in tumor suppression, particularly in acute promyelocytic leukemia, and their role in intrinsic antiviral defense against various pathogens. Despite significant advances in the field, key questions remain regarding the mechanistic triggers of PML-NB formation and their common roles across different pathologies. Further elucidation of these aspects may provide valuable insights for developing therapeutic approaches targeting the PML-NB axis in disease treatment.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Adaptations Determine the Evolutionary Trajectory of TOR Signaling in Diverse Eukaryotes.","authors":"Kyle Johnson, Dellaraam Pourkeramati, Ian Korf, Ted Powers","doi":"10.3390/biom15091295","DOIUrl":"10.3390/biom15091295","url":null,"abstract":"<p><p>Eukaryotes use diverse nutrient acquisition strategies, including autotrophy, heterotrophy, mixotrophy, and symbiosis, which shape the evolution of cell regulatory networks. The Target of Rapamycin (TOR) kinase is a conserved growth regulator that in most species functions within two complexes, TORC1 and TORC2. TORC1 is broadly conserved and uniquely sensitive to rapamycin, whereas the evolutionary distribution of TORC2 is less well-defined. We built a sensitive hidden Markov model (HMM)-based pipeline to survey core TORC1 and TORC2 components across more than 800 sequenced eukaryotic genomes spanning multiple major supergroups. Both complexes are present in early-branching lineages, consistent with their presence in the last eukaryotic common ancestor, followed by multiple lineage-specific losses of TORC2 and, more rarely, TORC1. A striking pattern emerges in which TORC2 is uniformly absent from photosynthetic autotrophs derived from primary endosymbiosis and frequently lost in those derived from secondary or tertiary events. In contrast, TORC2 is consistently retained in mixotrophs, which obtain carbon from both photosynthesis and environmental uptake, and in free-living obligate heterotrophs. These findings suggest that TORC2 supports heterotrophic metabolism and is often dispensable under strict autotrophy. Our results provide a framework for the evolutionary divergence of TOR signaling and highlight metabolic and ecological pressures that shape TOR complex retention across eukaryotes.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elderberry-Based Multifunctional Prebiotic Systems Prepared via Spray Drying.","authors":"Anna Gościniak, Lidia Tajber, Piotr Szulc, Andrzej Miklaszewski, Tomasz M Karpiński, Judyta Cielecka-Piontek","doi":"10.3390/biom15091289","DOIUrl":"10.3390/biom15091289","url":null,"abstract":"<p><p>Elderberry (<i>Sambucus nigra</i> L.) is recognized as a rich source of anthocyanins and other bioactives with antioxidant and antidiabetic potential, and is increasingly explored as a functional ingredient in nutraceuticals. However, cultivar-dependent variability can strongly influence chemical composition and bioactivity, underscoring the need for careful selection of plant material prior to formulation. In the present study, twelve genotypes of elderberry were compared in terms of total polyphenols, antioxidant activity, and antiglycation potential. Based on the overall profile, 'Samyl 1' was advanced to formulation trials. Spray-dried carrier systems were produced using galactooligosaccharides (GOS) or chitooligosaccharides (COS), with or without colloidal silica. GOS-based powders retained anthocyanins at levels approaching theoretical values and exhibited superior thermal stability, as evidenced by differential scanning calorimetry, thermogravimetric analysis, and degradation-kinetic modeling, whereas COS matrices provided less effective stabilization. Incorporation of silica significantly enhanced technological properties, improving recovery, reducing agglomeration, and increasing flowability, without compromising anthocyanin content. All powders displayed low moisture (2.5-7.1%), favorable morphology, and preserved functional activity, aligning with stability requirements for shelf-stable plant extracts. Overall, the study demonstrates that strategic cultivar selection combined with GOS-silica carrier systems enables the production of stable elderberry powders that maintain high anthocyanin content and bioactivity. Such multifunctional ingredients couple prebiotic functionality with efficient delivery of polyphenols, highlighting their potential in nutraceutical and pharmaceutical formulations.</p>","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}