Biomolecules最新文献_第10页

Modulating Effect of Carbohydrate Antigen 125 on the Prognostic Value of High-Sensitivity C-Reactive Protein in Heart Failure. 碳水化合物抗原125对心衰患者高敏c反应蛋白预后的调节作用。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-30 DOI: 10.3390/biom15091260

Enrique Santas, Arancha Martí-Martínez, Elena Revuelta-López, Sandra Villar, Rafael de la Espriella, Patricia Palau, Pau Llàcer, Gema Miñana, Enrique Rodriguez-Borja, Arturo Carratalá, Arantxa Gonzalez, Antoni Bayés-Genís, Juan Sanchis, Julio Núñez

{"title":"Modulating Effect of Carbohydrate Antigen 125 on the Prognostic Value of High-Sensitivity C-Reactive Protein in Heart Failure.","authors":"Enrique Santas, Arancha Martí-Martínez, Elena Revuelta-López, Sandra Villar, Rafael de la Espriella, Patricia Palau, Pau Llàcer, Gema Miñana, Enrique Rodriguez-Borja, Arturo Carratalá, Arantxa Gonzalez, Antoni Bayés-Genís, Juan Sanchis, Julio Núñez","doi":"10.3390/biom15091260","DOIUrl":"10.3390/biom15091260","url":null,"abstract":"Inflammation and congestion are key pathophysiological processes in heart failure (HF). Our aim was to evaluate the potential modulatory effect of carbohydrate antigen 125 (CA125) on inflammation, assessed by high-sensitivity C-reactive protein (hs-CRP). We analyzed a cohort of 4043 consecutive patients in whom hs-CRP and CA125 levels were measured during a hospitalization for acute HF. Multivariate Cox regression models were applied to assess the association between the biomarkers and all-cause mortality and death/HF rehospitalization at 6 months. In multivariable analysis, a significant interaction between hs-CRP and CA125 was observed for both outcomes (p-value for interaction = 0.036 and <0.001, respectively). hs-CRP was significantly associated with an increased risk of death (HR = 1.27; 95% CI 1.16-1.41; p < 0.001) and death/HF rehospitalization (HR = 1.18; 95% CI 1.09-1.28; p < 0.001) if CA125 > 35 U/mL. In contrast, hs-CRP was not predictive of events when CA125 ≤ 35 U/mL. In conclusion, in patients with acute HF, the association between hs-CRP and clinical outcomes was modulated by CA125 levels. hs-CRP was associated with a higher risk of events only in patients with elevated CA125. These findings support a potential modulatory and amplifying role for CA125 in the inflammatory response in HF.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Microgravity on the Structure and Function of Cardiomyocytes. 微重力对心肌细胞结构和功能的影响。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-30 DOI: 10.3390/biom15091261

Luis Fernando González-Torres, Daniela Grimm, Marcus Krüger

{"title":"The Effects of Microgravity on the Structure and Function of Cardiomyocytes.","authors":"Luis Fernando González-Torres, Daniela Grimm, Marcus Krüger","doi":"10.3390/biom15091261","DOIUrl":"10.3390/biom15091261","url":null,"abstract":"Spaceflight and microgravity (μg) environments induce numerous cardiovascular changes that affect cardiac structure and function, and understanding these effects is essential for astronaut health and tissue engineering in space. This review compiles and analyzes over 30 years of research on the impact of real and simulated μg on cardiomyocytes. A comprehensive literature search was conducted across five databases, and 62 eligible studies involving cardiac cells under μg or spaceflight conditions were compiled and analyzed. Despite the great heterogeneity in terms of cardiac model, microgravity platform, and exposure duration, multiple studies consistently reported alterations in Ca2+ handling, metabolism, contractility, and gene expression. Three-dimensional human-induced pluripotent stem cell-derived cardiomyocyte (HiPSC-CM) models generally showed enhanced tissue maturation and proliferation parameters, suggesting potential therapeutic benefits, while 2D models mostly exhibited stress-related dysfunction. In vivo simulated microgravity studies, such as the hindlimb unloading (HU) model, show structural and functional cardiac remodeling, and real μg studies confirmed various effects seen under the HU model in multiple rodent species. Thus, μg exposure consistently induces cardiac changes at the cellular and molecular level, while model choice, microgravity platform, and exposure duration critically influence the outcomes.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerol Kinase 2 as a Metabolic Sentinel for Human Sperm Motility and Male Fertility. 甘油激酶2作为人类精子活力和男性生育能力的代谢哨兵。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091249

João S Oliveira, Rúben J Moreira, Ana D Martins, Marco G Alves, Pedro F Oliveira

{"title":"Glycerol Kinase 2 as a Metabolic Sentinel for Human Sperm Motility and Male Fertility.","authors":"João S Oliveira, Rúben J Moreira, Ana D Martins, Marco G Alves, Pedro F Oliveira","doi":"10.3390/biom15091249","DOIUrl":"10.3390/biom15091249","url":null,"abstract":"Male infertility affects 8-12% of couples worldwide and is solely responsible in up to 30% of cases. Assisted Reproductive Technologies (ARTs) provide potential solutions, particularly in conditions where spermatozoa display structural abnormalities or impaired motility, such as asthenozoospermia. Sperm metabolism demonstrates remarkable flexibility, shifting between glycolysis and oxidative phosphorylation to produce ATP required for motility. Glycerol kinase 2 (GK2) phosphorylates glycerol in the sperm midpiece, generating glycerol-3-phosphate, a key intermediate in glycolysis, lipid metabolism, and oxidative phosphorylation. The localization of GK2 suggests not only a regulatory role in sperm metabolism but also a possible association with VDAC proteins, contributing to ADP-ATP exchange between the cytosol and mitochondria. Elucidating the role of GK2 in spermatozoa is of particular relevance, as this enzyme not only contributes to key metabolic pathways but may also interact with VDAC proteins, influencing mitochondrial function and energy exchange. Such interactions could play a pivotal role in regulating sperm motility. A deeper understanding of these mechanisms could position GK2 as a valuable biomarker: in scenarios where GK2-VDAC interactions are confirmed, it may guide optimized sperm selection methods in ARTs, whereas the absence or impairment of such interactions could serve as a diagnostic indicator in asthenozoospermic men.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiofilm Inhibitor Ferulic Acid as an Antibacterial Synergist Against Escherichia coli. 抗生素膜抑制剂阿魏酸对大肠杆菌的抑菌增效作用。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091253

Zhijin Zhang, Jing Xu, Xiaojuan Wei, Rongbin Hu, Zhen Zhu, Zixuan Shang, Weiwei Wang, Bing Li, Yubin Bai, Jiyu Zhang

{"title":"Antibiofilm Inhibitor Ferulic Acid as an Antibacterial Synergist Against Escherichia coli.","authors":"Zhijin Zhang, Jing Xu, Xiaojuan Wei, Rongbin Hu, Zhen Zhu, Zixuan Shang, Weiwei Wang, Bing Li, Yubin Bai, Jiyu Zhang","doi":"10.3390/biom15091253","DOIUrl":"10.3390/biom15091253","url":null,"abstract":"Escherichia coli (E. coli) is a severe foodborne pathogen, and the formation of its biofilm can enhance bacterial virulence and reduce antibiotic sensitivity, posing a significant threat to human and animal health. Ferulic Acid (FA) is a natural active product that has been proven to possess various biological activities, including anti-inflammatory, antioxidant, and antitumor properties. This study evaluated the inhibitory effect of FA on the biofilm formation of E. coli through crystal violet (CV) staining and scanning electron microscopy (SEM) and investigated the synergistic effect of FA with antibiotics, using the alamar blue (AB) assay. In addition, the regulatory effect of FA on the transcription of biofilm-related genes was analyzed using qRT-PCR technology. The results showed that FA could significantly inhibit biofilm formation, reduce the production of extracellular polymeric substances (EPS), and weaken bacterial motility, without affecting bacterial growth and metabolic activity. qRT-PCR analysis revealed that FA significantly downregulated the expression of curli-related gene csgD, flagella-related genes (flhC, flhD, and motA), and type I fimbriae gene fimA, while upregulating the transcription of c-di-GMP-related genes (pdeR, pdeA, and dosP). It is noteworthy that FA exhibits significant synergistic antibacterial effects when combined with clinically commonly used antibiotics, including sodium fosfomycin, ceftriaxone, gentamicin, and tetracycline, with the most prominent synergistic effect observed in the combination of FA and sodium fosfomycin. These results confirm that FA possesses notable anti-biofilm activity and novel synergistic antibacterial properties, providing a potential therapeutic strategy for treating E. coli infections.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Functional Studies on Key Epigenetic Regulators in Asthma. 哮喘关键表观遗传调控因子的结构与功能研究。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091255

Muhammad Fakhar, Mehreen Gul, Wenjin Li

{"title":"Structural and Functional Studies on Key Epigenetic Regulators in Asthma.","authors":"Muhammad Fakhar, Mehreen Gul, Wenjin Li","doi":"10.3390/biom15091255","DOIUrl":"10.3390/biom15091255","url":null,"abstract":"Asthma is a chronic inflammatory airway disease influenced by both genetic and environmental factors. Recent insights have underscored the pivotal role of epigenetic regulation in the pathogenesis and heterogeneity of asthma. This review focuses on key epigenetically important regulators categorized as writers, erasers, and readers that govern DNA methylation, histone modifications, and RNA modifications. These proteins modulate gene expression without altering the underlying DNA sequence, thereby influencing immune responses, airway remodeling, and disease severity. We highlight the structural and functional dynamics of histone acetyltransferases (e.g., p300/CBP), histone deacetylases (e.g., SIRT family), DNA methyltransferases (DNMT1, DNMT3A), demethylases (TET1), and methyl-CpG-binding proteins (MBD2) in shaping chromatin accessibility and transcriptional activity. Additionally, the m6A RNA modification machinery including METTL3, METTL14, FTO, YTHDF1/2, IGF2BP2, and WTAP is explored for its emerging significance in regulating post-transcriptional gene expression during asthma progression. Structural characterizations of these proteins reveal conserved catalytic domains and interaction motifs, mirroring their respective families such as SIRTs, p300/CBP, DNMT1/3A, and YTHDF1/2 critical to their epigenetic functions, offering mechanistic insight into their roles in airway inflammation and immune modulation. By elucidating these pathways, this review provides a framework for the development of epigenetic biomarkers and targeted therapies. Future directions emphasize phenotype-specific epigenomic profiling and structure-guided drug design to enable precision medicine approaches in asthma management.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ashes to Rashes: An Exploration of the Intersection Between Smoking and Cutaneous Lupus Erythematosus. 从灰烬到皮疹：吸烟与皮肤红斑狼疮交叉关系的探讨。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091250

Rafael O Homer, Ahmed Eldaboush, Darae Kang, Nada S Ahmed, Touraj Khosravi-Hafshejani, Ming-Lin Liu, Victoria P Werth

{"title":"Ashes to Rashes: An Exploration of the Intersection Between Smoking and Cutaneous Lupus Erythematosus.","authors":"Rafael O Homer, Ahmed Eldaboush, Darae Kang, Nada S Ahmed, Touraj Khosravi-Hafshejani, Ming-Lin Liu, Victoria P Werth","doi":"10.3390/biom15091250","DOIUrl":"10.3390/biom15091250","url":null,"abstract":"Cutaneous lupus erythematosus is an autoimmune skin disorder with a known association with cigarette smoking. Smokers with cutaneous lupus have a worse disease course and may be refractory to treatments. Despite many studies documenting this association, minimal work exists examining the molecular drivers of these clinical differences. This review delves into how cigarette smoke may influence key immunopathogenic pathways in cutaneous lupus, including oxidative stress, interferon signaling, inflammatory cell recruitment, extracellular vesicles, and immune regulation. Additionally, factors such as epigenetics and heat injury are considered as well. Here, we integrate the existing and emerging literature on the pathophysiology of cutaneous lupus with known effects of cigarette smoke on the skin and immune system and propose hypotheses that may explain clinical differences in smokers. Understanding the molecular underpinnings of these differences may yield a clearer picture of the disease and more effective treatment strategies.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Aging in the CNS: Unravelling Implications for Neurological Health and Disease. 中枢神经系统的线粒体衰老：揭示神经健康和疾病的含义。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091252

Davide Steffan, Camilla Pezzini, Martina Esposito, Anais Franco-Romero

{"title":"Mitochondrial Aging in the CNS: Unravelling Implications for Neurological Health and Disease.","authors":"Davide Steffan, Camilla Pezzini, Martina Esposito, Anais Franco-Romero","doi":"10.3390/biom15091252","DOIUrl":"10.3390/biom15091252","url":null,"abstract":"Mitochondrial aging plays a central role in the functional decline of the central nervous system (CNS), with profound consequences for neurological health. As the brain is one of the most energy-demanding organs, neurons are particularly susceptible to mitochondrial dysfunction that arises with aging. Key features of mitochondrial aging include impaired mitochondrial dynamics, reduced mitophagy, increased production of reactive oxygen species (ROS), and accumulation of mitochondrial DNA (mtDNA) mutations. These alterations dramatically compromise neuronal bioenergetics, disrupt synaptic integrity, and promote oxidative stress and neuroinflammation, paving the path for the development of neurodegenerative diseases. This review also examines the complex mechanisms driving mitochondrial aging in the central nervous system (CNS), including the disruption of mitochondrial-organelle communication, and explores how mitochondrial dysfunction contributes to neurodegenerative diseases, such as Alzheimer's, Parkinson's, Huntington's, and amyotrophic lateral sclerosis. By synthesizing current evidence and identifying key knowledge gaps, we emphasize the urgent need for targeted strategies to restore mitochondrial function, maintain cognitive health, and delay or prevent age-related neurodegeneration.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Yeast One-Hybrid Library of Dendrobium huoshanense and Screening of Potential Transcription Factors Regulating DhPMM Gene Expression. 霍山石斛酵母单杂交文库的构建及调控DhPMM基因表达的潜在转录因子筛选

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091251

Jing Wu, Shuting Wang, Shihai Xing, Daiyin Peng

{"title":"Construction of Yeast One-Hybrid Library of Dendrobium huoshanense and Screening of Potential Transcription Factors Regulating DhPMM Gene Expression.","authors":"Jing Wu, Shuting Wang, Shihai Xing, Daiyin Peng","doi":"10.3390/biom15091251","DOIUrl":"10.3390/biom15091251","url":null,"abstract":"Dendrobium huoshanense, an endangered orchid species, is renowned for its polysaccharides with vast pharmacological significance in stems. Phosphomannomutase (PMM) critically regulates polysaccharide accumulation. Transcriptional regulation of DhPMM remains poorly characterized. This study employed a yeast one-hybrid (Y1H) system to identify upstream regulators of DhPMM. The 2.15 kb DhPMM promoter was cloned, revealing multiple stress- and hormone-responsive cis-elements (e.g., ABRE, MYC, ERF). A high-complexity Y1H library (3.60 × 109 CFU) was constructed with insert sizes averaging 1-2 kb. Screening using aureobasidin A (AbA)-resistant Y1HGold [pAbAi-DhPMM] identified 11 candidate clones, including four transcription factor families (DOF, NAC, ERF, BES1). Interactions were rigorously confirmed by pairwise Y1H showing AbA-resistant growth and dual-luciferase assays demonstrating DhPMM activation. This represents the first functional cDNA library resource for D. huoshanense and identification of TFs interacting with DhPMM. The discovery of TFs belonging to DOF, NAC, ERF, and BES1 families as DhPMM regulators elucidated the transcriptional network underlying polysaccharide biosynthesis. This establishes a transcriptional framework for engineering polysaccharide biosynthesis in D. huoshanense.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repetitive DNAs and Karyotype Evolution in Phyllostomid Bats (Chiroptera: Phyllostomidae). 毛蝗目：毛蝗科蝙蝠的重复dna和核型进化。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091248

Geize Aparecida Deon, Tariq Ezaz, José Henrique Forte Stornioli, Rodrigo Zeni Dos Santos, Anderson José Baia Gomes, Príncia Grejo Setti, Edivaldo Herculano Correa de Oliveira, Fábio Porto-Foresti, Ricardo Utsunomia, Thomas Liehr, Marcelo de Bello Cioffi

{"title":"Repetitive DNAs and Karyotype Evolution in Phyllostomid Bats (Chiroptera: Phyllostomidae).","authors":"Geize Aparecida Deon, Tariq Ezaz, José Henrique Forte Stornioli, Rodrigo Zeni Dos Santos, Anderson José Baia Gomes, Príncia Grejo Setti, Edivaldo Herculano Correa de Oliveira, Fábio Porto-Foresti, Ricardo Utsunomia, Thomas Liehr, Marcelo de Bello Cioffi","doi":"10.3390/biom15091248","DOIUrl":"10.3390/biom15091248","url":null,"abstract":"Bats are great models for studying repetitive DNAs due to their compact genomes and extensive chromosomal rearrangements. Here, we investigated the repetitive DNA content of two phyllostomid bat species, Artibeus lituratus (2nn = 30♀/31♂) and Carollia perspicillata (2n = 20♀/21♂), both harboring a multiple XY1Y2 sex chromosome system. Satellite DNA (satDNA) libraries were isolated and characterized, revealing four and ten satDNA families in A. lituratus and C. perspicillata, respectively. These sequences, along with selected microsatellites, were in situ mapped onto chromosomes in both species and phylogenetically related taxa. SatDNAs showed strong accumulation in centromeric and subtelomeric regions, especially pericentromeric areas. Cross-species mapping with C. perspicillata-derived probes indicated terminal localization patterns in other bat species, suggesting conserved distribution. Microsatellites co-localized with 45S rDNA clusters on the neo-sex chromosomes. Additionally, genomic hybridization revealed a male-specific signal on the Y1 chromosome, pointing to potential sex-linked repetitive regions. These findings confirm that bat genomes display relatively low amounts of repetitive DNA compared to other mammals and underscore the role of these elements in genome organization and sex chromosome evolution in phyllostomid bats.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomarker Signatures in Time-Course Progression of Neuropathic Pain at Spinal Cord Level Based on Bioinformatics and Machine Learning Analysis. 基于生物信息学和机器学习分析的脊髓水平神经性疼痛时程进展的生物标志物特征。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-08-29 DOI: 10.3390/biom15091254

Kexin Li, Ruoxi Wang, He Zhu, Bei Wen, Li Xu, Yuguang Huang

{"title":"Biomarker Signatures in Time-Course Progression of Neuropathic Pain at Spinal Cord Level Based on Bioinformatics and Machine Learning Analysis.","authors":"Kexin Li, Ruoxi Wang, He Zhu, Bei Wen, Li Xu, Yuguang Huang","doi":"10.3390/biom15091254","DOIUrl":"10.3390/biom15091254","url":null,"abstract":"Neuropathic pain (NP) is a debilitating chronic pain condition with complex molecular mechanisms and inadequate therapeutic solutions. This study aims to identify temporal transcriptomic changes in NP using multiple bioinformatics and machine learning algorithms. A total of 10 mouse samples (5 per group) were harvested at each time point (day three, day seven, and day fourteen), following spared nerve injury and a sham operation. Differentially expressed gene (DEG) analysis and an intersection among the three time-point groups revealed 54 common DEGs. The GO and KEGG analyses mainly showed enrichment in terms of immune response, cell migration, and signal transduction functions. In addition, the interaction of the LASSO, RF, and SVM-RFE machine learning models on 54 DEGs resulted in Ngfr and Ankrd1. The cyan module in WGCNA was selected for a time-dependent upward trend in gene expression. Then, 172 genes with time-series signatures were integrated with 54 DEGs, resulting in 11 shared DEGs. Quantitative RT-PCR validated the temporal expressions of the above genes, most of which have not been reported yet. Additionally, immune infiltration analysis revealed significant positive correlations between monocyte abundance and the identified genes. The TF-mRNA-miRNA network and drug-target network revealed potential therapeutic drugs and posttranscriptional regulatory mechanisms. In conclusion, this study explores genes with time-series signatures as biomarkers in the development and maintenance of NP, potentially revealing novel targets for analgesics.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0