Repetitive DNAs and Karyotype Evolution in Phyllostomid Bats (Chiroptera: Phyllostomidae).

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2025-08-29 DOI:10.3390/biom15091248

Geize Aparecida Deon, Tariq Ezaz, José Henrique Forte Stornioli, Rodrigo Zeni Dos Santos, Anderson José Baia Gomes, Príncia Grejo Setti, Edivaldo Herculano Correa de Oliveira, Fábio Porto-Foresti, Ricardo Utsunomia, Thomas Liehr, Marcelo de Bello Cioffi

{"title":"Repetitive DNAs and Karyotype Evolution in Phyllostomid Bats (Chiroptera: Phyllostomidae).","authors":"Geize Aparecida Deon, Tariq Ezaz, José Henrique Forte Stornioli, Rodrigo Zeni Dos Santos, Anderson José Baia Gomes, Príncia Grejo Setti, Edivaldo Herculano Correa de Oliveira, Fábio Porto-Foresti, Ricardo Utsunomia, Thomas Liehr, Marcelo de Bello Cioffi","doi":"10.3390/biom15091248","DOIUrl":null,"url":null,"abstract":"Bats are great models for studying repetitive DNAs due to their compact genomes and extensive chromosomal rearrangements. Here, we investigated the repetitive DNA content of two phyllostomid bat species, Artibeus lituratus (2nn = 30♀/31♂) and Carollia perspicillata (2n = 20♀/21♂), both harboring a multiple XY1Y2 sex chromosome system. Satellite DNA (satDNA) libraries were isolated and characterized, revealing four and ten satDNA families in A. lituratus and C. perspicillata, respectively. These sequences, along with selected microsatellites, were in situ mapped onto chromosomes in both species and phylogenetically related taxa. SatDNAs showed strong accumulation in centromeric and subtelomeric regions, especially pericentromeric areas. Cross-species mapping with C. perspicillata-derived probes indicated terminal localization patterns in other bat species, suggesting conserved distribution. Microsatellites co-localized with 45S rDNA clusters on the neo-sex chromosomes. Additionally, genomic hybridization revealed a male-specific signal on the Y1 chromosome, pointing to potential sex-linked repetitive regions. These findings confirm that bat genomes display relatively low amounts of repetitive DNA compared to other mammals and underscore the role of these elements in genome organization and sex chromosome evolution in phyllostomid bats.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8000,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467311/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomolecules","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/biom15091248","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Bats are great models for studying repetitive DNAs due to their compact genomes and extensive chromosomal rearrangements. Here, we investigated the repetitive DNA content of two phyllostomid bat species, Artibeus lituratus (2nn = 30♀/31♂) and Carollia perspicillata (2n = 20♀/21♂), both harboring a multiple XY₁Y₂ sex chromosome system. Satellite DNA (satDNA) libraries were isolated and characterized, revealing four and ten satDNA families in A. lituratus and C. perspicillata, respectively. These sequences, along with selected microsatellites, were in situ mapped onto chromosomes in both species and phylogenetically related taxa. SatDNAs showed strong accumulation in centromeric and subtelomeric regions, especially pericentromeric areas. Cross-species mapping with C. perspicillata-derived probes indicated terminal localization patterns in other bat species, suggesting conserved distribution. Microsatellites co-localized with 45S rDNA clusters on the neo-sex chromosomes. Additionally, genomic hybridization revealed a male-specific signal on the Y₁ chromosome, pointing to potential sex-linked repetitive regions. These findings confirm that bat genomes display relatively low amounts of repetitive DNA compared to other mammals and underscore the role of these elements in genome organization and sex chromosome evolution in phyllostomid bats.

查看原文本刊更多论文

毛蝗目：毛蝗科蝙蝠的重复dna和核型进化。

蝙蝠由于其紧凑的基因组和广泛的染色体重排，是研究重复dna的伟大模型。本研究对两种具有XY1Y2多重性染色体系统的phyllostomid蝙蝠Artibeus lituratus （2n = 30♀/31♂）和Carollia perspicillata （2n = 20♀/21♂）的重复DNA含量进行了研究。通过对卫星DNA文库的分离和鉴定，分别获得了4个和10个卫星DNA家族。这些序列，连同选定的微卫星，被原位定位到物种和系统发育相关分类群的染色体上。在着丝粒区和亚端粒区，特别是在着丝粒区，有大量的satdna积累。利用spspicillata衍生探针进行的跨物种定位显示了其他蝙蝠物种的末端定位模式，表明其分布较为保守。微卫星与新性别染色体上的45S rDNA簇共定位。此外，基因组杂交揭示了Y1染色体上的男性特异性信号，指出了潜在的性别连锁重复区域。这些发现证实，与其他哺乳动物相比，蝙蝠基因组中重复DNA的数量相对较少，并强调了这些元素在层状目蝙蝠基因组组织和性染色体进化中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.