Biomolecules最新文献

Nanocomposite Biomaterials for Tissue-Engineered Hernia Repair: A Review of Recent Advances. 纳米复合生物材料用于组织工程疝气修复：最新进展综述。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-22 DOI: 10.3390/biom15091348

Octavian Andronic, Alexandru Cosmin Palcau, Alexandra Bolocan, Alexandru Dinulescu, Daniel Ion, Dan Nicolae Paduraru

{"title":"Nanocomposite Biomaterials for Tissue-Engineered Hernia Repair: A Review of Recent Advances.","authors":"Octavian Andronic, Alexandru Cosmin Palcau, Alexandra Bolocan, Alexandru Dinulescu, Daniel Ion, Dan Nicolae Paduraru","doi":"10.3390/biom15091348","DOIUrl":"10.3390/biom15091348","url":null,"abstract":"Hernia repair is among the most frequent procedures in general surgery, traditionally performed with synthetic meshes such as polypropylene. While effective in reducing recurrence, these materials are biologically inert and often trigger chronic inflammation, fibrosis, pain, and impaired abdominal wall function, with a significant impact on long-term quality of life. A comprehensive literature search was conducted in PubMed, Web of Science, and Scopus databases, and relevant preclinical, clinical, and review articles were synthesized within a narrative review framework. Recent advances in tissue engineering propose a shift from passive reinforcement to regenerative strategies based on biomimetic scaffolds, nanomaterials, and nanocomposites that replicate the extracellular matrix, enhance cell integration, and provide controlled drug delivery. Nanotechnology enables localized release of anti-inflammatory, antimicrobial, and pro-angiogenic agents, while electrospun nanofibers and composite scaffolds improve strength and elasticity. In parallel, 3D printing allows for patient-specific implants with tailored architecture and regenerative potential. Although preclinical studies show encouraging results, clinical translation remains limited by cost, regulatory constraints, and long-term safety uncertainties. Overall, these innovations highlight a transition toward personalized and regenerative hernia repair, aiming to improve durability, function, and patient quality of life.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYC and Metabolomics: Can We Use What We Know for DLBCL Subtyping and Diagnosis? MYC和代谢组学：我们可以利用我们所知道的DLBCL亚型和诊断吗？

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-20 DOI: 10.3390/biom15091346

Adrian Florentin Suman, Davide De Luca, Melania Gaggini, Francesco Cucco

{"title":"MYC and Metabolomics: Can We Use What We Know for DLBCL Subtyping and Diagnosis?","authors":"Adrian Florentin Suman, Davide De Luca, Melania Gaggini, Francesco Cucco","doi":"10.3390/biom15091346","DOIUrl":"10.3390/biom15091346","url":null,"abstract":"Diffuse large B-cell lymphoma (DLBCL) is a molecular and clinical heterogenous entity, and, over the past 30 years, many efforts have been made in trying to dissect this diverseness and identify biomarkers capable of efficiently stratifying DLBCL patients and spotting the ones showing a worse clinical outcome. Despite the achievement in this research field, only a few biomarkers have been validated and introduced in a clinical setting. Among those, approximately 5-15% of DLBCL cases harbor MYC gene translocations, often involving immunoglobulin genes as a translocation partner, and concomitant point mutations, correlating with a poor response to standard therapies. However, given the difficulty in detecting these abnormalities requiring specialized techniques and high-quality specimens, the use of metabolomics (i.e., the study of small metabolites in body fluids and tissues) can offer a useful alternative for the identification of high-risk DLBCL patients. Amino acids (AAs) are metabolites essential in the process of tumorigenesis and can increase immune escape and drug resistance. Therefore, we review the use of metabolomics to improve the diagnosis and prognosis in DLBCL patients in relation to the MYC role in the regulation of amino acid metabolism, as these metabolites may be used as potential biomarkers in a clinical environment.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutraceuticals Against Oxidative Stress in Allergic Diseases. 抗过敏性疾病氧化应激的营养品。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-20 DOI: 10.3390/biom15091347

Marilena Di Salvo, Alessandra Ventre, Enrica Dato, Marco Casciaro, Sebastiano Gangemi

{"title":"Nutraceuticals Against Oxidative Stress in Allergic Diseases.","authors":"Marilena Di Salvo, Alessandra Ventre, Enrica Dato, Marco Casciaro, Sebastiano Gangemi","doi":"10.3390/biom15091347","DOIUrl":"10.3390/biom15091347","url":null,"abstract":"Antioxidant mechanisms consist of both enzymatic and non-enzymatic compounds, which can be either endogenous or exogenous and play a crucial role in counteracting oxidative stress. These compounds are primarily obtained through the diet. Vegetables, plants, and fruits contain a wide range of alkaloids, polyphenols, and terpenoids, collectively referred to as \"phytochemicals.\" Many of these substances are responsible for the beneficial properties of fruits and vegetables, which are essential components of a healthy lifestyle, contributing to the prevention of chronic diseases and the promotion of longevity. Nutraceuticals are bioactive substances present in food-or its components-that exert positive effects on health and may help prevent or treat various disorders. In this review, we examine the main applications of nutraceuticals in allergic disorders. The literature reports numerous studies on exogenous dietary antioxidant supplementation in various allergic conditions, including bronchial asthma, atopic dermatitis, food allergies, allergic rhino-conjunctivitis, urticaria, and angioedema. In some of these conditions, promising results have been observed. These positive outcomes are generally associated with a reduction in oxidative stress markers, enhancement of antioxidant systems, and, in some cases, anti-inflammatory effects. The administration of exogenous substances through food derivatives or dietary supplements, when scientifically standardized, has been proven to be effective. However, further large-scale, unbiased studies are needed-particularly those that include a broader range of oxidative stress biomarkers.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Glucagon-like Peptide-1 Receptor Agonists (GLP-1RAs) in Patients with Chronic Heart Failure. 胰高血糖素样肽1受体激动剂（GLP-1RAs）在慢性心力衰竭患者中的作用

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-19 DOI: 10.3390/biom15091342

Pasqual Llongueras-Espí, Elena García-Romero, Josep Comín-Colet, José González-Costello

{"title":"Role of Glucagon-like Peptide-1 Receptor Agonists (GLP-1RAs) in Patients with Chronic Heart Failure.","authors":"Pasqual Llongueras-Espí, Elena García-Romero, Josep Comín-Colet, José González-Costello","doi":"10.3390/biom15091342","DOIUrl":"10.3390/biom15091342","url":null,"abstract":"Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are widely used in the management of type 2 diabetes and obesity due to their metabolic benefits. Beyond weight loss and glycemic control, emerging evidence suggests they may also exert cardioprotective effects. In the context of heart failure (HF), particularly HF with preserved ejection fraction (HFpEF), GLP-1RAs have been associated with improvement in symptoms, physical capacity, biomarkers, and structural cardiac remodeling. These benefits appear to be independent of weight loss, suggesting additional mechanisms including anti-inflammatory effects, improved myocardial metabolism or modulation of epicardial adipose tissue. However, current data largely come from non-HF dedicated trials, with limited standardization of the HF phenotype. Results are overall inconsistent and may suggest potential harm in some cases, particularly in HF with reduced ejection fraction (HFrEF). This review aims to summarize the current evidence on the role of GLP-1RAs in heart failure, explore possible underlying mechanisms and highlight key gaps in knowledge.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NLRP3 Inflammasome in Stress-Related Neuropsychiatric Disorders: Mechanisms of Neuron-Microglia-Astrocyte Crosstalk, HPA Axis Dysregulation, and Therapeutic Perspective. 应激相关神经精神疾病中的NLRP3炎性体：神经元-小胶质细胞-星形胶质细胞串扰、HPA轴失调的机制和治疗观点。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-19 DOI: 10.3390/biom15091344

Izabela Woźny-Rasała, Ewa Alicja Ogłodek

引用次数: 0

Investigation of the Effect of 2,3-Dihydrobenzoic Acid Acid (2,3-DHBA) on the Lipid Profiles of MCF-7 and MDA-MB-231 Human Breast Cancer Cells via an Untargeted Lipidomic Approach. 2,3-二氢苯甲酸（2,3- dhba）对MCF-7和MDA-MB-231人乳腺癌细胞脂质谱影响的非靶向脂质组学研究

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-19 DOI: 10.3390/biom15091341

Büşra Daş, Serap Şahin

{"title":"Investigation of the Effect of 2,3-Dihydrobenzoic Acid Acid (2,3-DHBA) on the Lipid Profiles of MCF-7 and MDA-MB-231 Human Breast Cancer Cells via an Untargeted Lipidomic Approach.","authors":"Büşra Daş, Serap Şahin","doi":"10.3390/biom15091341","DOIUrl":"10.3390/biom15091341","url":null,"abstract":"Breast cancer (BC) is a primary cause of cancer-related mortality in women, making the development of novel therapeutic strategies essential. Altered lipid metabolism is a recognized hallmark of cancer, presenting a key therapeutic vulnerability. This study investigated the cytotoxic effects of the natural phenolic compound 2,3-DHBA on MCF-7 (luminal A) and MDA-MB-231 (triple-negative) human breast cancer cells and characterized the associated changes in their lipid profiles via an untargeted lipidomic approach. The in vitro cytotoxicity of 2,3-DHBA was assessed using the MTT assay at 24, 48, and 72 h against both cancer cell lines and non-cancerous L-929 fibroblasts. Following treatment with the 48-h IC50 concentrations (8.61 mM for MCF-7, 5.84 mM for MDA-MB-231), total lipids were extracted and analyzed. The results showed that 2,3-DHBA exerted potent time- and dose-dependent cytotoxic effects against both BC cell lines, with significantly higher selectivity for cancer cells over healthy fibroblasts. The more aggressive MDA-MB-231 line exhibited greater sensitivity. The lipidomic analysis revealed that 2,3-DHBA induced profound cell-specific alterations across all major lipid classes, including fatty acids, glycerolipids (GLs), glycerophospholipids (GPs), and sphingolipids (SPs). These changes suggest a multi-pronged mechanism involving the disruption of membrane integrity through GP remodeling, the attenuation of survival signaling via the GL network, and a critical shift in the sphingolipid rheostat towards pro-apoptotic ceramide accumulation. This study establishes a direct link between the cytotoxic activity of 2,3-DHBA and its ability to comprehensively reprogram the cancer cell lipidome, highlighting its potential as a sophisticated metabolic modulator for breast cancer therapy.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives on Alzheimer's Disease Treatment Based on Counteracting Oxidative Stress. 基于抗氧化应激的阿尔茨海默病治疗展望

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-19 DOI: 10.3390/biom15091345

Rafał Bilski, Stanisław Dąbkowski, Igor Kozieł, Michał Kozicki, Anna Małachowska, Mikołaj Przygocki, Oliwia Tyska

{"title":"Perspectives on Alzheimer's Disease Treatment Based on Counteracting Oxidative Stress.","authors":"Rafał Bilski, Stanisław Dąbkowski, Igor Kozieł, Michał Kozicki, Anna Małachowska, Mikołaj Przygocki, Oliwia Tyska","doi":"10.3390/biom15091345","DOIUrl":"10.3390/biom15091345","url":null,"abstract":"Alzheimer's disease (AD) is a progressive neurodegenerative disorder and one of the most pressing global health challenges. Increasing evidence highlights oxidative stress as a key factor in its pathogenesis, contributing to amyloid-β accumulation, tau hyperphosphorylation, neuroinflammation, and mitochondrial dysfunction. Oxidative stress markers, detected in the bodily fluids of AD patients, are considered promising diagnostic and prognostic tools. Despite extensive research, currently available therapies remain largely symptomatic, which emphasizes the need to develop novel, disease-modifying strategies. The aim of this review is to summarize current knowledge on the role of oxidative stress in the pathogenesis of AD and to evaluate therapeutic approaches aimed at its reduction. We discuss molecular mechanisms linking reactive oxygen species to neurodegeneration and present pharmacological strategies such as monoamine oxidase inhibitors and multifunctional agents, as well as natural antioxidants, dietary interventions, and novel therapeutic technologies. We pay particular attention to their efficacy, limitations, and translational challenges. A more profound understanding of oxidative stress-related mechanisms may facilitate the development of combined antioxidant, anti-inflammatory, and neuroprotective approaches, offering new perspectives for delaying disease progression and improving patient outcomes.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction Between Heparan Sulfate Oligosaccharide and the Receptor-Binding Domain of the Wild-Type and Omicron Variant of the SARS-CoV-2 Spike Protein. 硫酸乙酰肝素寡糖与SARS-CoV-2刺突蛋白野生型和组粒变体受体结合域的相互作用

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-19 DOI: 10.3390/biom15091343

Marco Mandalari, Michela Parafioriti, Minghong Ni, Francesca Benevelli, Monica Civera, Stefano Elli, Marco Guerrini

{"title":"Interaction Between Heparan Sulfate Oligosaccharide and the Receptor-Binding Domain of the Wild-Type and Omicron Variant of the SARS-CoV-2 Spike Protein.","authors":"Marco Mandalari, Michela Parafioriti, Minghong Ni, Francesca Benevelli, Monica Civera, Stefano Elli, Marco Guerrini","doi":"10.3390/biom15091343","DOIUrl":"10.3390/biom15091343","url":null,"abstract":"Heparan sulfate proteoglycans serve as initial attachment sites for several viruses and bacteria. Recent studies suggest that SARS-CoV-2 similarly exploits these glycosaminoglycans, facilitating conformational changes in the spike protein that promote the interaction between the receptor-binding domain (S1-RBD) and the cellular angiotensin-converting enzyme 2 receptor (ACE2), thereby triggering the virus internalization process. The molecular details that drive this process, particularly the co-receptor role of heparan sulfate (HS), remain incompletely understood. The interaction between an HS hexasaccharide (hexa) and the N343 glycosylated S1-RBD of the wild-type (WT) and Omicron variant of SARS-CoV-2 was investigated. The conformational properties of hexa with these S1-RBDs in unbound and bound states are explored using multiple independent MD simulations; the protein binding epitope of hexa, as well as the details of its interaction with S1-RBD of the Omicron variant, are characterized by comparing experimental and theoretical 1H STD NMR signals. This investigation identifies the role played by the glycosyl moiety at N343 in potentially affecting this interaction in both WT and Omicron S1-RBD, explaining the observed low specificity and multi-modal nature of the interaction between HS oligosaccharides and these S1-RBDs.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145173175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Years 2015-2025 as a Prospective Decade for the Identification of Specific Methylation Biomarkers of Prostate Cancer. 2015-2025年是前列腺癌特异性甲基化生物标志物鉴定的未来十年。

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-18 DOI: 10.3390/biom15091334

Zohair Selmani, Paul Peixoto, Alexis Overs, Eric Hervouet

引用次数: 0

Exploring the Inhibitory Potential of Six Porphyrin Compounds Against α-Amylase and α-Glucosidase Linked to Diabetes. 六种卟啉化合物对糖尿病相关α-淀粉酶和α-葡萄糖苷酶抑制作用的研究

IF 4.8 2区生物学

Biomolecules Pub Date : 2025-09-18 DOI: 10.3390/biom15091338

Shuo Zhang, Zi Liu, Qiurui Ma, Yangyuxin Liu, Shuren Yin, Zhihan Zhou, Jie Zhou, Helong Bai, Tianjiao Li

{"title":"Exploring the Inhibitory Potential of Six Porphyrin Compounds Against α-Amylase and α-Glucosidase Linked to Diabetes.","authors":"Shuo Zhang, Zi Liu, Qiurui Ma, Yangyuxin Liu, Shuren Yin, Zhihan Zhou, Jie Zhou, Helong Bai, Tianjiao Li","doi":"10.3390/biom15091338","DOIUrl":"10.3390/biom15091338","url":null,"abstract":"Diabetes mellitus is a characteristic metabolic disorder with diverse complications. α-Amylase and α-glucosidase, as key digestive enzymes regulating blood glucose, are important targets for diabetes prevention and management through their inhibition. This study investigated the inhibitory effects of six porphyrin compounds (TAPP, TCPP, THPP, Cu-TCPP, Fe-TCPP, Ni-TCPP) on two enzymes through in vitro inhibition assays, spectroscopic experiments, and molecular docking techniques. All six compounds effectively inhibited the activities of both enzymes. For α-amylase, the inhibitory potency (IC50 = 13.03-245.04 μg/mL) followed the order TAPP > THPP > TCPP > Fe-TCPP > Ni-TCPP > Cu-TCPP. All six compounds exhibited more potent inhibitory activity against α-glucosidase (IC50 = 0.24-25.43 μg/mL), with potency in the order of THPP > Ni-TCPP > Fe-TCPP > TCPP > Cu-TCPP > TAPP. Fluorescence quenching experiments revealed that all compounds statically quenched the intrinsic fluorescence of both enzymes (with Fe-TCPP exhibiting static-dominant mixed quenching against α-amylase), indicating complex formation. These interactions significantly altered the enzymes' conformations, the microenvironments of Tyr/Trp residues, and secondary structure content, consequently reducing their catalytic activity. By examining the inhibitory impact of porphyrin compounds on α-amylase and α-glucosidase, this research establishes a vital experimental and theoretical basis for diabetes therapeutics.","PeriodicalId":8943,"journal":{"name":"Biomolecules","volume":"15 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0