Biogerontology最新文献

{"title":"From sick care to healthspan: educating the longevity physician for health maintenance and health promotion.","authors":"Dominik Thor, David Barzilai, Yu-Xuan Lyu, Luiza Spiru","doi":"10.1007/s10522-025-10371-3","DOIUrl":"10.1007/s10522-025-10371-3","url":null,"abstract":"<p><p>This paper focuses specifically on the education and upskilling of medical doctors, proposing how longevity-related competencies can be incorporated first through continuing medical education (CME) pathways and, eventually, into formal medical curricula. As longevity medicine evolves from research into clinical practice, education has emerged as its defining challenge. While the science of ageing advances rapidly, most physicians remain unprepared to translate biological, technological, and preventive insights into responsible medical care. Emerging foundational fields such as biogerontology, which investigates the biological mechanisms of ageing across organisms, and its clinically oriented derivative geroscience, have created new expectations for translational capacity in healthcare. This paper extends previous conceptual work by outlining potential educational domains, proposing a structured education, and outlining a pedagogical and accreditation model for incorporating longevity-related competencies into medical training pathways. The framework integrates geroscience, digital diagnostics, and healthspan-oriented care within established medical education and quality assurance standards. By exploring potential pathways from postgraduate to continuing education, longevity medicine may contribute to more coherent and evidence-aligned practice. Education may represent one enabling factor in efforts to shift, where feasible, from predominantly reactive care toward more proactive approaches to health maintenance.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"22"},"PeriodicalIF":4.1,"publicationDate":"2025-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12702796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics research in aging: a bibliometric and visualized analysis of evolution and emerging trends (1998-2024 and early 2025).","authors":"Yuyuan Gao, Yang Yang, Xinli Xue, Yinyan Xu, Jinghua Yang","doi":"10.1007/s10522-025-10366-0","DOIUrl":"10.1007/s10522-025-10366-0","url":null,"abstract":"<p><p>This study aims to analyze the global research landscape and identify emerging trends in research hotspots, key technologies, and clinical applications in proteomics research in aging from 1998 to June 20, 2025. Publications related to aging and proteomics from 1998 to June 20, 2025 were retrieved from the Web of Science Core Collection. A bibliometric analysis was conducted using VOSviewer, CiteSpace, and R 4.3.3 to evaluate publication trends, research collaborations, and emerging topics. A total of 3,638 studies were included in the analysis. The USA, China, and Germany led in publication volume with 983, 829, and 227 articles respectively. Harvard University was the most prolific institution with 306 publications, followed by University of California System and Chinese Academy of Sciences. Key research was published in high-impact journals such as Journal of Proteome Research, Aging Cell, and Proteomics. Luigi Ferrucci, and D. Allan Butterfield were the most influential authors. Cluster analysis identified five research hotspots: protein expression and cellular senescence mechanisms, age-related diseases and neurodegeneration, cellular processes and molecular mechanisms, stress response and longevity mechanisms, and advanced proteomics technologies and biomarker discovery. Burst keyword analysis revealed recent research hotspots including health, dementia, extracellular vesicles and receptor. This study demonstrates that aging proteomics research has matured into distinct yet interconnected domains spanning basic molecular mechanisms, clinical disease applications, and technological innovations, reflecting the field's evolution toward translational and precision medicine approaches for age-related conditions. Future research directions may focus on clinical translation of aging biomarkers and development of precision medicine approaches for age-related diseases.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"21"},"PeriodicalIF":4.1,"publicationDate":"2025-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12702804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota as a causal mediator linking inflammatory cytokines and ageing phenotypes.","authors":"Qiyu Liu, Weidong Li, Wuliang Diao, Wenjun Shi, Li Yu, Yuchen Cai, Xueyao Cai","doi":"10.1007/s10522-025-10369-x","DOIUrl":"10.1007/s10522-025-10369-x","url":null,"abstract":"<p><p>Population ageing is a global phenomenon with significant implications for public health. Research has highlighted a relationship between gut microbiota, inflammatory cytokines, and ageing, yet the underlying causal mechanisms remain elusive. This study uses Mendelian randomization (MR) analysis to investigate causal relationships between gut microbiota, inflammatory cytokines, and ageing phenotypes. We leveraged the summary statistics of gut microbiota (n = 5959), circulating inflammatory cytokines (n = 8293), and three ageing phenotypes including telomere length (n = 472,174), facial ageing (n = 423,999), and frailty index (n = 175,226). We performed bidirectional MR analyses to explore the causal effects of gut microbiota and inflammatory cytokines on ageing, and mediation analyses to discover potential mediating gut microbiota and inflammatory cytokines. Our findings suggest that there are causal interactions between gut microbiota, inflammatory cytokines, and ageing. Notably, the abundance of GCA-900066575 sp900066385 appears to mediate the M-CSF pathway to facial ageing. The current MR study provides evidence supporting causal relationships between inflammatory cytokines and ageing and potential mediating gut microbiota, which are critical to advancing our understanding of the ageing process and developing effective interventions.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"20"},"PeriodicalIF":4.1,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145740950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic exposure alters the LEAP-2/ghrelin axis and anti-inflammatory tone in aged male rat liver and adipose tissue.","authors":"Rabia Ilgin, Oya Sayin, Mehmet Ates, Erhan Caner Akkaya, Ferda Hosgorler","doi":"10.1007/s10522-025-10368-y","DOIUrl":"10.1007/s10522-025-10368-y","url":null,"abstract":"<p><p>Liver-expressed antimicrobial peptide-2 (LEAP-2), the endogenous antagonist of the ghrelin receptor (GHSR1a), counterbalances ghrelin in an energy- and inflammation-dependent manner. Aging is accompanied by endocrine and immunometabolic shifts that may influence this axis. We investigated whether a short course of broad-spectrum antibiotics (vancomycin-metronidazole-neomycin-ampicillin; VMNA) alters LEAP-2 and ghrelin levels in the liver and epididymal white adipose tissue (WAT) of aged male rats, and whether these changes coincide with modifications in IL-10, TNF-α, and IL-1β. Antibiotic treatment lowered LEAP-2 in both liver and WAT. Ghrelin decreased in both tissues, but the reduction reached significance only in WAT, whereas the hepatic decrease was nonsignificant. Consequently, the LEAP-2/ghrelin ratio declined in the liver and showed a nonsignificant upward trend in WAT. Inflammatory profiling revealed that IL-10 decreased in both tissues, whereas TNF-α and IL-1β remained unchanged. These findings demonstrate that even a one-week antibiotic regimen induces tissue-specific alterations in the LEAP-2/ghrelin axis-characterized by reduced hepatic LEAP-2 signaling, suppressed adipose ghrelin, and diminished anti-inflammatory tone. Overall, the data suggest that aged male rats exhibit heightened vulnerability to antibiotic-induced perturbations in LEAP-2/ghrelin regulation, underscoring the interplay between microbiota-related influences, inflammaging, and age-associated metabolic imbalance.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"19"},"PeriodicalIF":4.1,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145740822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verbascoside from Callicarpa nudiflora Hook extends lifespan in Caenorhabditis elegans via SKN-1 pathway activation.","authors":"Yuhang Liu, Jiale Wu, Zhiyang Ding, Liru Chen, Manyu Liu, Baoli Li, Xiaokang Li, Jian Li, Wenwen Liu","doi":"10.1007/s10522-025-10365-1","DOIUrl":"10.1007/s10522-025-10365-1","url":null,"abstract":"<p><p>Aging involves a progressive decline in physiological function, leading to organ damage and age-related chronic diseases. Natural products derived from traditional herbs represent a valuable resource for identifying anti-aging compounds and potential lead candidates. In this study, a screen of an herbal library using Caenorhabditis elegans (C. elegans) identified the 75% ethanol extract of Callicarpa nudiflora Hook (LWLY01) as a potent lifespan-extending agent. Further fractionation revealed that its ethyl acetate extract (LWLY03) exhibited significant anti-aging activity, albeit with complex composition. Through systematic isolation and identification, verbascoside was determined to be a key active constituent. Verbascoside extended the lifespan of C. elegans and improved healthspan parameters, including motility and resistance to osmotic and thermal stress. Mutant lifespan assays demonstrated that verbascoside acts through activation of the SKN-1 signaling pathway, thereby strengthening resistance to aging-associated oxidative stress. Additionally, verbascoside was found to regulate multiple aging-related processes, such as stress response, oxidative damage, and cellular homeostasis. These findings highlight verbascoside as a promising natural compound for mitigating aging phenotypes and preventing age-related diseases.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"18"},"PeriodicalIF":4.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics profiling reveals mitochondrial dysfunction and epigenetic dysregulation in postoperative cognitive dysfunction: identification of novel biomarkers in an aged mouse model.","authors":"Li Hu, Hongmei Zhou, Liuyi Song, Jian Lu, Shulei Zhang, Xiaoyan Ye, Qinghe Zhou, Zhengliang Ma","doi":"10.1007/s10522-025-10357-1","DOIUrl":"10.1007/s10522-025-10357-1","url":null,"abstract":"<p><p>Postoperative cognitive dysfunction (POCD) is a prevalent neurocognitive disorder in elderly patients following surgery and anesthesia. However, the underlying mechanisms remain poorly understood, and treatment options are limited. This study applied a multi-omics strategy, combining cognitive-behavioral assessments with proteomic and metabolomic profiling, to uncover the molecular basis of POCD in an aged mouse model. Behavioral assessments, including the Morris Water Maze (MWM), Open Field Test (OFT), and Novel Object Recognition (NOR), revealed significant cognitive deficits in POCD mice. Proteomic analysis identified 103 differentially expressed proteins (DEPs), with 34 upregulated and 69 downregulated, alongside significant correlations among 16 proteins. Enrichment analysis indicated disturbances in mitochondrial energy metabolism and epigenetic regulation, linked to neurodegenerative pathways. Metabolomic profiling detected 99 metabolites, with 66 upregulated and 33 downregulated, confirming their differential expression between groups. Correlation analysis between DEPs and metabolites led to the identification of nine key proteins (PSB9, COA7, PFD2, CUTA, LEG1, LEG9, BET1L, CHIL3, KV5AG) as potential biomarkers and therapeutic targets for POCD. These findings emphasize the complex relationship between cognitive dysfunction, molecular alterations, and metabolic disruptions in POCD, suggesting a multifactorial pathogenesis that warrants further investigation to develop targeted interventions.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"17"},"PeriodicalIF":4.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-tissue integration of immunocytes and inflammaging biomarkers predicts biological age through LASSO-optimized modeling.","authors":"Jiawei Yang, Haichen Zhang, Qiong Zhang, Tao Zhou, Lening Chen, Qianqian Xiao, Shusheng Luo, Qinghe Meng, Jianjun Jiang, Weidong Hao, Xuetao Wei","doi":"10.1007/s10522-025-10364-2","DOIUrl":"10.1007/s10522-025-10364-2","url":null,"abstract":"<p><p>Immunosenescence, a recognized hallmark of aging, is characterized by imbalances in immunocyte populations and a state of chronic inflammation. However, the tissue-specific dynamics of these changes and their potential as predictive biomarkers for aging remain poorly characterized. In this study, we established a multi-tissue immunological signature as a robust predictor of biological age by integrating immunocyte and cytokine profiling. Using Sprague-Dawley (SD) rats from five age groups (1-12 months), we systematically quantified 45 immunocyte subsets across peripheral blood, mesenteric lymph nodes, thymus, and spleen using flow cytometry, and profiled 22 serum cytokines/chemokines via Flexible Multi-Analyte Profiling (xMAP). Firstly, classic age-dependent shifts were observed across our rat samples, including progressive thymic involution and depletion of peripheral T-cells. Cytokine levels exhibited age-related chronic inflammation progression, marked by elevated IL-1α, granulocyte colony-stimulating factor (G-CSF), and TNF-α. To integrate these multidimensional datasets into a predictive aging metric, we employed Least Absolute Shrinkage and Selection Operator (LASSO) regression, selecting 22 biomarkers through regularization (λ = 0.111). The integrated model combining cellular and cytokine data demonstrated superior performance (training R² = 0.957, validation R² = 0.887), outperforming single-modality models based on immunocytes or cytokines. Notably, splenic parameters dominated the aging signature, contributing seven biomarkers representing 60% of model weight-particularly Th-cell expansion and Tc-cell depletion. Peripheral blood Th-cell proportion emerged as another key predictor. Our findings position the spleen as a critical aging hub and identify peripheral/splenic Th-cell modulation as promising therapeutic targets for age-related immune dysfunction, revealing novel mechanistic insights into aging-associated immune remodeling.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"15"},"PeriodicalIF":4.1,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing chronomics of clock, immune and Parkinson's disease-associated genes in SCN and SN with aging in male Wistar rats: ketogenic diet intervention.","authors":"Sushree Abhidhatri Sharma, Anita Jagota","doi":"10.1007/s10522-025-10346-4","DOIUrl":"10.1007/s10522-025-10346-4","url":null,"abstract":"<p><p>The hypothalamic suprachiasmatic nucleus (SCN) functions as the central circadian pacemaker, synchronizing peripheral clocks through oscillations in core clock genes and proteins. Circadian disruption contributes to immunosenescence, aging, and neurodegenerative disorders such as Parkinson's disease (PD). Previous work from our group demonstrated age-related changes in circadian rhythms of clock genes, protein levels, and serotonin metabolism in the SCN and substantia nigra (SN) of male Wistar rats. This study examined the age of onset for circadian misalignment in clock (rBmal1, rCry1, rCry2, rPer1, rPer2), immune (rCox2, rIl1β, rIl4, rTgfβ1), and PD-associated (rLrrk2, rPark2, rPark7, rSnca) genes in SCN and SN. Male Wistar rats aged 3 (adult), 12 (middle-aged), and 24 (aged) months were studied. In SCN, rPark2 decreased and rSnca increased in 12 months and 24 months, while rCry1 and rPer2 were elevated in 12 months. Rhythmicity of rTgfβ1 declined in 24 months. In SN, rBmal1 rhythmicity was abolished in 24 months, while rPark2 lost rhythmicity in 12 months and 24 months. rSnca and rIl1β were elevated in 24 months. Misalignments in rCry1, rPer2, rIl4, rIl1β, rTgfβ1, and rLrrk2 in SCN, and rCry2, rIl4, rLrrk2, rPark2, and rSnca in SN appeared by middle age. A ketogenic diet intervention (KDI) resulted in modulation of rhythmic expression of rPer2, rSnca, rCry1, rTgfβ1, and rPark2 in SCN and improved rPark2, rSnca, and rIl1β in SN. These findings indicate that translationally, circadian misalignment in PD-related genes emerges early, suggesting its potential as a biomarker for preclinical PD. Moreover, dietary strategies such as KDI highlight promising non-pharmacological approaches to preserve circadian integrity, delay neurodegeneration, and guide personalized interventions in at-risk individuals.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"16"},"PeriodicalIF":4.1,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of high-intensity interval training and resistance training on the follistatin and myostatin levels in gastrocnemius muscle of aged female rats.","authors":"Seyyed Moslem Asadpour, Farhad Daryanoosh, Amir Hossein Zarifkar","doi":"10.1007/s10522-025-10363-3","DOIUrl":"10.1007/s10522-025-10363-3","url":null,"abstract":"<p><p>Myostatin and follistatin are the regulators of muscle growth and pivotal proteins that regulate muscle tissue function. An integrated approach is HIIT and resistance training provides a holistic strategy for promoting healthy aging and maintaining functional abilities, potentially through the modulation of myostatin and follistatin levels. This study aims to assess the effect of high-intensity interval training and resistance training on myostatin and follistatin protein concentrations in aged rats' serum and muscle tissue. In this study, 20-month-old female Sprague-Dawley rats were used in three groups: (1) Control (Con), (2) Resistance training (RT), and (3) High-intensity interval training (HIIT). The HIIT and resistance training protocols were carried out for 8 weeks and three sessions per week. The results showed serum levels and muscle tissue content of myostatin increased in the RT compared to the control group (p = 0.0001 and p = 0.04). The muscle tissue content of follistatin increased in the HIIT compared to the control group (p = 0.03). There is a significant difference in serum levels and muscle tissue content of follistatin between HIIT and RT groups (p = 0.0001 and p = 0.001). According to the roles of myostatin and follistatin in regulating muscle hypertrophy, present research shows HIIT has more effects on follistatin levels and resistance training has more effects on myostatin levels. This can indicate that according to the number of training sessions, HIIT can be a better and newer treatment method for older people.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"14"},"PeriodicalIF":4.1,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of extracellular vesicles-associated protein abundance and aggregation during aging and disease in C. elegans.","authors":"Prasun Kumar Bhunia, Prasad Kasturi","doi":"10.1007/s10522-025-10362-4","DOIUrl":"10.1007/s10522-025-10362-4","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) contribute to the maintenance of organism-wide proteostasis by mediating intercellular communication. Loss of proteostasis and altered intercellular communication are associated with aging and age-related diseases, suggesting key roles for EVs. However, it is unclear how the proteome of the EVs changes with age. To identify EV-associated proteins (EVAPs) and their fate with age, we curated publicly available EV proteome data from C. elegans model organism and human. Our analysis reveals that EVs carry proteins with diverse functions, including those involved in protein quality control. We found that abundance of the EVAPs changes significantly with age, heat stress, pathogen infections and diseases. Many of these EVAPs also aggregate with age and overlap with Aβ-driven protein aggregates. Further, we identified human orthologs of C. elegans EVAPs from human brain tissues affected with Alzheimer's disease and breast cancer. This meta-analysis highlights EVs proteome composition, their abundance changes, and aggregation during aging, stress, infection and disease conditions. Overall, this study provides new insights into the dynamics of EV proteins during aging and may possibly help in identifying potential biomarkers for age-related diseases.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"27 1","pages":"13"},"PeriodicalIF":4.1,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}