Biogerontology最新文献_第9页

The potential longevity-promoting hypoxic-hypercapnic environment as a measure for radioprotection. 作为放射防护措施的潜在长寿促进缺氧-高二氧化碳浓度环境。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-10-01 Epub Date: 2024-08-20 DOI: 10.1007/s10522-024-10129-3

Elroei David, Marina Wolfson, Khachik K Muradian, Vadim E Fraifeld

引用次数: 0

Network pharmacology-based approach to investigate the molecular targets and molecular mechanisms of Rosmarinus officinalis L. for treating aging-related disorders. 基于网络药理学的方法，研究迷迭香治疗衰老相关疾病的分子靶点和分子机制。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-10-01 Epub Date: 2024-07-17 DOI: 10.1007/s10522-024-10122-w

Amisha Bisht, Disha Tewari, Sanjay Kumar, Subhash Chandra

{"title":"Network pharmacology-based approach to investigate the molecular targets and molecular mechanisms of Rosmarinus officinalis L. for treating aging-related disorders.","authors":"Amisha Bisht, Disha Tewari, Sanjay Kumar, Subhash Chandra","doi":"10.1007/s10522-024-10122-w","DOIUrl":"10.1007/s10522-024-10122-w","url":null,"abstract":"Aging, a natural biological process, presents challenges in maintaining physiological well-being and is associated with increased vulnerability to diseases. Addressing aging mechanisms is crucial for developing effective preventive and therapeutic strategies against age-related ailments. Rosmarinus officinalis L. is a medicinal herb widely used in traditional medicine, containing diverse bioactive compounds that have been studied for their antioxidant and anti-inflammatory properties, which are associated with potential health benefits. Using network pharmacology, this study investigates the anti-aging function and underlying mechanisms of R. officinalis. Through network pharmacology analysis, the top 10 hub genes were identified, including TNF, CTNNB1, JUN, MTOR, SIRT1, and others associated with the anti-aging effects. This analysis revealed a comprehensive network of interactions, providing a holistic perspective on the multi-target mechanism underlying Rosemary's anti-aging properties. GO and KEGG pathway enrichment analysis revealed the relevant biological processes, molecular functions, and cellular components involved in treating aging-related conditions. KEGG pathway analysis shows that anti-aging targets of R. officinalis involved endocrine resistance, pathways in cancer, and relaxin signaling pathways, among others, indicating multifaceted mechanisms. Genes like MAPK1, MMP9, and JUN emerged as significant players. These findings enhance our understanding of R. officinalis's potential in mitigating aging-related disorders through multi-target effects on various biological processes and pathways. Such approaches may reduce the risk of failure in single-target and symptom-based drug discovery and therapy.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"793-808"},"PeriodicalIF":4.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of mTOR prevents glucotoxicity-mediated increase of SA-beta-gal, p16^INK4a, and insulin hypersecretion, without restoring electrical features of mouse pancreatic islets. 抑制 mTOR 可防止葡萄糖毒性介导的 SA-beta-gal、p16INK4a 和胰岛素分泌过多的增加，但不会恢复小鼠胰岛的电特性。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-10-01 Epub Date: 2024-05-15 DOI: 10.1007/s10522-024-10107-9

Tereso J Guzmán, Nina Klöpper, Carmen M Gurrola-Díaz, Martina Düfer

{"title":"Inhibition of mTOR prevents glucotoxicity-mediated increase of SA-beta-gal, p16INK4a, and insulin hypersecretion, without restoring electrical features of mouse pancreatic islets.","authors":"Tereso J Guzmán, Nina Klöpper, Carmen M Gurrola-Díaz, Martina Düfer","doi":"10.1007/s10522-024-10107-9","DOIUrl":"10.1007/s10522-024-10107-9","url":null,"abstract":"An over-activation of the mechanistic target of rapamycin (mTOR) pathway promotes senescence and age-related diseases like type 2 diabetes. Besides, the regenerative potential of pancreatic islets deteriorates with aging. Nevertheless, the role of mTOR on senescence promoted by metabolic stress in islet cells as well as its relevance for electrophysiological aspects is not yet known. Here, we investigated whether parameters suggested to be indicative for senescence are induced in vitro in mouse islet cells by glucotoxicity and if mTOR inhibition plays a protective role against this. Islet cells exhibit a significant increase (~ 76%) in senescence-associated beta-galactosidase (SA-beta-gal) activity after exposure to glucotoxicity for 72 h. Glucotoxicity does not markedly influence p16INK4a protein within 72 h, but p16INK4a levels increase significantly after a 7-days incubation period. mTOR inhibition with a low rapamycin concentration (1 nM) entirely prevents the glucotoxicity-mediated increase of SA-beta-gal and p16INK4a. At the functional level, reactive oxygen species, calcium homeostasis, and electrical activity are disturbed by glucotoxicity, and rapamycin fails to prevent this. In contrast, rapamycin significantly attenuates the insulin hypersecretion promoted by glucotoxicity by modifying the mRNA levels of Vamp2 and Snap25 genes, related to insulin exocytosis. Our data indicate an influence of glucotoxicity on pancreatic islet-cell senescence and a reduction of the senescence markers by mTOR inhibition, which is relevant to preserve the regenerative potential of the islets. Decreasing the influence of mTOR on islet cells exposed to glucotoxicity attenuates insulin hypersecretion, but is not sufficient to prevent electrophysiological disturbances, indicating the involvement of mTOR-independent mechanisms.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"819-836"},"PeriodicalIF":4.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the human health and lifespan: a targeted strategy emphasizing statins. 提高人类健康水平和寿命：强调他汀类药物的针对性战略。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-10-01 Epub Date: 2024-05-29 DOI: 10.1007/s10522-024-10112-y

Edward Calabrese, Peter Pressman

{"title":"Enhancing the human health and lifespan: a targeted strategy emphasizing statins.","authors":"Edward Calabrese, Peter Pressman","doi":"10.1007/s10522-024-10112-y","DOIUrl":"10.1007/s10522-024-10112-y","url":null,"abstract":"There has been substantial research interest in finding activities/agents that slow the onset and reduce the severity of numerous age-related diseases/conditions. This assessment indicates that the most studied agent intended to promote health in human population investigations for a broad spectrum of diseases are the statins, with large-scale epidemiological studies addressing numerous health endpoints. The key findings are that statin treatment consistently reduces the occurrence and attenuates the course of numerous non-communicable and contagious pathologies and numerous types of cancer with high mortality rates by about 20-50%. That one agent could affect such a broad based and consistently positive trends in epidemiological studies is unexpected and impressive, along with consistent cell and animal model research. Underlying mechanisms have been proposed that significantly contribute to the spectrum of salutary effects of statins, especially the capacity to activate Nrf2 showing hormetic dose responses in multiple organs and cell types, due to its bioavailability and broad tissue distribution. The widespread use of statins, which has the capacity to enhance human health span, should be considered for experimental exploration as a novel public health strategy that includes practical approaches for reduction of the most common adverse effects of this class of drugs including myalgia/myopathy and transaminitis.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"883-890"},"PeriodicalIF":4.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a new human senescent skin cell marker ribonucleoside-diphosphate reductase subunit M2 B 鉴定一种新的人类衰老皮肤细胞标记核糖核苷-二磷酸还原酶亚基 M2 B

IF 4.5 4区医学

Biogerontology Pub Date : 2024-09-11 DOI: 10.1007/s10522-024-10135-5

Kento Takaya, Kazuo Kishi

{"title":"Identification of a new human senescent skin cell marker ribonucleoside-diphosphate reductase subunit M2 B","authors":"Kento Takaya, Kazuo Kishi","doi":"10.1007/s10522-024-10135-5","DOIUrl":"https://doi.org/10.1007/s10522-024-10135-5","url":null,"abstract":"In skin aging, it has been hypothesized that aging fibroblasts accumulate within the epidermal basal layer, dermis, and subcutaneous fat, causing abnormal tissue remodeling and extracellular matrix dysfunction, thereby inducing an aging-related secretory phenotype (SASP). A new treatment for skin aging involves the specific elimination of senescent skin cells, especially fibroblasts within the dermis and keratinocytes in the basal layer. This requires the identification of specific protein markers of senescent cells, such as ribonucleoside-diphosphate reductase subunit M2 B (RRM2B), which is upregulated in various malignancies in response to DNA stress damage. However, the behavior and role of RRM2B in skin aging remain unclear. Therefore, we examined whether RRM2B functions as a senescence marker using a human dermal fibroblast model of aging. In a model of cellular senescence induced by replicative aging and exposure to ionizing radiation or UVB, RRM2B was upregulated at the gene and protein levels. This was correlated with decreased uptake of the senescence-associated β-galactosidase activity and proliferation marker bromodeoxyuridine. RRM2B upregulation was concurrent with the increased expression of SASP factor genes. Furthermore, using fluorescence flow cytometry, RRM2B-positive cells were recovered more frequently in the aging cell population. In aging human skin, RRM2B was also found to be more abundant in the dermis and epidermal basal layer than other proteins. Therefore, RRM2B may serve as a clinical marker to identify senescent skin cells.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"58 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142202115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of social partners: investigating mixed-strain housing effects on aging in female mice 社会伙伴的影响：研究混合品系饲养对雌性小鼠衰老的影响

IF 4.5 4区医学

Biogerontology Pub Date : 2024-09-11 DOI: 10.1007/s10522-024-10139-1

Chih-Lin Lee, Yu-Chiao Lin, Tsung-Han Kuo

{"title":"The impact of social partners: investigating mixed-strain housing effects on aging in female mice","authors":"Chih-Lin Lee, Yu-Chiao Lin, Tsung-Han Kuo","doi":"10.1007/s10522-024-10139-1","DOIUrl":"https://doi.org/10.1007/s10522-024-10139-1","url":null,"abstract":"Aging is a multifaceted process characterized by the gradual decline of physiological functions and can be modulated by various internal and external factors. While social interactions have been shown to affect behaviors and physiology in different species, the impact of social partners on aging-related phenotypes and lifespan in mice remains understudied. To address this question, we investigated various aging-related traits and lifespan in two mouse strains, C57BL/6J and BALB/c, under two different housing conditions: mixed-strain and same-strain housing. Analyses using a Generalized Linear Model revealed significant differences between the two strains in several phenotypes, including metabolic, anxiety-like, and electrocardiographic traits. However, surprisingly, housing conditions did not significantly affect most of the examined parameters, including overall lifespan. Only 3 out of 25 traits—body weight change in a metabolic cage, running wheel activity, and survival days of a quartiles of mice with middle lifespans—were influenced by housing conditions in a strain-dependent manner. Together, our study suggested a minimal influence of co-housing with social partners from different genetic backgrounds on aging-related phenotypes. This result demonstrates the feasibility of mixed housing for mouse husbandry and, more importantly, provides valuable insights for future research on the social influences on the aging process in mice.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"47 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142202116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The opposite aging effect to single cell transcriptome profile among cell subsets 细胞亚群中的单细胞转录组特征与衰老效应相反

IF 4.5 4区医学

Biogerontology Pub Date : 2024-09-11 DOI: 10.1007/s10522-024-10138-2

Daigo Okada

引用次数: 0

Geroscience: just another name or is there more to it? 老年科学：只是另一个名称，还是有更多内涵？

IF 4.4 4区医学

Biogerontology Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1007/s10522-024-10105-x

Simon Okholm

引用次数: 0

Sustained exposure to high glucose induces differential expression of cellular senescence markers in murine macrophages but impairs immunosurveillance response to senescent cells secretome. 持续暴露于高葡萄糖可诱导小鼠巨噬细胞中细胞衰老标记物的差异表达，但会损害对衰老细胞分泌组的免疫监视反应。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-08-01 Epub Date: 2024-01-19 DOI: 10.1007/s10522-024-10092-z

Bhawna Diwan, Rahul Yadav, Rohit Goyal, Rohit Sharma

{"title":"Sustained exposure to high glucose induces differential expression of cellular senescence markers in murine macrophages but impairs immunosurveillance response to senescent cells secretome.","authors":"Bhawna Diwan, Rahul Yadav, Rohit Goyal, Rohit Sharma","doi":"10.1007/s10522-024-10092-z","DOIUrl":"10.1007/s10522-024-10092-z","url":null,"abstract":"The influence of chronic diseases on various facets of macrophage cellular senescence is poorly understood. This study evaluated the impact of chronic hyperglycemia on the induction of cellular senescence and subsequent immunosurveillance functions in RAW264.7 macrophages. Macrophages were cultured under normal glucose (NG; 5 mM), high glucose (HG; 20 mM), and very high glucose (VHG; 40 mM) conditions and assessed for markers of cellular senescence. Hyperglycemia induced strong upregulation of SA-β-gal activity, and loss of PCNA and Lamin B1 gene expression while markers of cell cycle arrest generally decreased. Non-significant changes in SASP-related proteins were observed while ROS levels slightly decreased and mitochondrial membrane potential increased. Protein concentration on the exosome membrane surface and their stability appeared to increase under hyperglycemic conditions. However, when macrophages were exposed to the secretory media (SM) of senescent preadipocytes, a dramatic increase in the levels of all inflammatory proteins was recorded especially in the VHG group that was also accompanied by upregulation of NF-κB and NLRP3 gene expression. SM treatment to hyperglycemic macrophages activated the TLR-2/Myd88 pathway but decreased the expression of scavenger receptors RAGE, CD36, and Olr-1 while CD44 and CXCL16 expression increased. On exposure to LPS, a strong upregulation in NO, ROS, and inflammatory cytokines was observed. Together, these results suggest that primary markers of cellular senescence are aberrantly expressed under chronic hyperglycemic conditions in macrophages with no significant SASP activation. Nonetheless, hyperglycemia strongly deregulates macrophage functions leading to impaired immunosurveillance of senescent cells and aggravation of inflamm-aging. This work provides novel insights into how hyperglycemia-induced dysfunctions can impact the potency of macrophages to manage senescent cell burden in aging tissues.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"627-647"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139490349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piperine improves the health span of Drosophila melanogaster with age- and sex-specific effect. 胡椒碱能改善黑腹果蝇的健康寿命，并具有年龄和性别特异性效应。

IF 4.4 4区医学

Biogerontology Pub Date : 2024-08-01 Epub Date: 2024-03-28 DOI: 10.1007/s10522-024-10100-2

Hye-Yeon Lee, Ji-Hyeon Lee, Jisun Baek, Kyung A Cho, Kyung-Jin Min

{"title":"Piperine improves the health span of Drosophila melanogaster with age- and sex-specific effect.","authors":"Hye-Yeon Lee, Ji-Hyeon Lee, Jisun Baek, Kyung A Cho, Kyung-Jin Min","doi":"10.1007/s10522-024-10100-2","DOIUrl":"10.1007/s10522-024-10100-2","url":null,"abstract":"Piperine, a dietary phytochemical isolated from the Piper species, has been used as a natural medicine for pain, flu, and fever in ancient China and India. Although the health benefits of piperine have been widely studied, research on its effect on aging is limited. This study aimed to determine whether piperine has the potential to mitigate aging-related changes in the fruit fly (Drosophila melanogaster), which is an excellent model organism for studies on aging. The experiments were conducted using the newly eclosed or 30-day-old D. melanogaster wild-type strain Cantonized-white. Piperine was dissolved in 99% ethanol and added to the sucrose-yeast medium at a final concentration of 10, 35, 70, or 100 μM. The study examined the effects of piperine supplementation on the lifespan of D. melanogaster and other physiological functions, such as fecundity, feeding, lipid content, and resistance to environmental stress. Log-rank tests, Shapiro-Wilk test, F-test, t-test, or Wilcoxon rank sum test were used to analyze the data. Piperine failed to change the lifespan and body weight, but increased the fecundity and decreased the feeding rate in one-week-old flies. However, when piperine was fed to 30-day-old flies, it increased the lifespan of male flies and the fecundity and feeding rate of female flies. These results indicate that piperine can improve the health of aged flies. The findings suggest that piperine has age-dependent and sex-specific anti-aging effects in fruit flies.","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":" ","pages":"665-677"},"PeriodicalIF":4.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140317738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0