Biogerontology最新文献

{"title":"Zuogui and Yougui pills extend lifespan and improve ageing biomarkers via kaempferol-mediated mitophagy in Caenorhabditis elegans.","authors":"Wendi Chen, Shuang Liu, Guoqiang Xu, Xin Liu, Yuxuan Shi, Guolong Wang, Yunna Ning, Zhiming Lu, Yongzhi Cao, Yueran Zhao","doi":"10.1007/s10522-025-10317-9","DOIUrl":"https://doi.org/10.1007/s10522-025-10317-9","url":null,"abstract":"<p><p>Zuogui pill (ZGP) and Yougui pill (YGP) are classical kidney-tonifying formulas in Traditional Chinese Medicine, widely used clinically but with their potential to delay ageing and improve ageing biomarkers remaining unclear. This study combined network pharmacology and Caenorhabditis elegans models to investigate the anti-ageing effects and mechanisms of ZGP and YGP. Both formulas significantly extended lifespan (ZGP dose-dependently at 5-20 mg/mL; YGP at 20 mg/mL) and improved ageing biomarkers, as evidenced by enhanced motility, reduced lipofuscin accumulation and endogenous ROS levels, and increased resistance to heat and oxidative stress. Network analysis identified quercetin and kaempferol as the top-ranked shared active components. Subsequent experimental validation demonstrated that kaempferol (0.05-0.2 mM) replicated these pro-longevity effects and was shown to act by inducing mitophagy: it triggered an initial decrease followed by a long-term increase in mitochondrial content, concomitant with upregulated expression of mitophagy genes. Crucially, the lifespan-extending effects of kaempferol, ZGP, and YGP were completely abolished in bec-1 and pink-1 null mutants. This study establishes that ZGP and YGP delay ageing and improve ageing biomarkers in C. elegans by activating BEC-1/PINK-1-dependent mitophagy. Kaempferol was identified as a major active component mediating this effect, highlighting a key mechanism for the pro-longevity properties of these traditional formulas.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"172"},"PeriodicalIF":4.1,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blueberries for brainpower: A systematic review and meta-analysis with Bayesian post hoc analysis of RCTS exploring cognitive function in the elderly with prior cognitive decline.","authors":"Ana Beatriz Nardelli da Silva, Gabriel Moraes de Oliveira, Mariano Gallo Ruelas, Maitê Silva Martins Gadelha, Ana Clara Felix de Farias Santos, Fernanda Valeriano Zamora","doi":"10.1007/s10522-025-10308-w","DOIUrl":"https://doi.org/10.1007/s10522-025-10308-w","url":null,"abstract":"<p><p>Blueberries are anthocyanin-rich fruits widely consumed by the general population, with well-established health benefits on the endocrine and cardiovascular systems attributed to their potent anti-inflammatory properties. However, the potential impact of blueberry consumption on cognitive function in elderly individuals with prior cognitive decline, such as Alzheimer's disease and dementia, remains insufficiently explored in the literature. Therefore, we aimed to evaluate the potential effects of chronic blueberry consumption on cognitive performance in this population through various memory assessment tools. We searched PubMed, Embase, and Cochrane for randomized controlled trials (RCTs) evaluating the effect of chronic blueberry consumption on cognitive function. We pooled standard mean differences (SMD) and 95% confidence interval (CI) using a random-effects model. We identified nine eligible RCTs involving 513 patients. In elderly individuals with mild cognitive impairment (MCI) and subjective cognitive decline, blueberry intake showed a statistically significant improvement in episodic memory. (SMD = 0.34; 95% CI 0.11 to 0.57; p < 0.05). The subgroup analysis revealed that diagnostic objetivity was not a statistically significant effect modifier. Blueberry intake was also associated with improved language memory in MCI patients (SMD = 0.30; 95% CI 0.01 to 0.60; p < 0.05). No improvements were seen in processing speed (SMD = - 0.33; 95% CI - 0.85 to 0.19, p > 0.05), recognition memory (SMD = 0.14; 95% CI - 0.17 to 0.46, p > 0.05), visuospatial learning (SMD = 0.32; 95% CI - 0.16 to 0.79, p > 0.05) and working memory (SMD = 0.09; 95% CI - 0.21 to 0.39, p > 0.05). Chronic blueberry intake may improve episodic memory in the elderly with MCI and subjective cognitive decline, and also language in the elderly with MCI. These findings should be confirmed in further multicenter trials to confirm generalizability and long-term impact.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"171"},"PeriodicalIF":4.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-Hydroxybutyrate but not NMN supplementation mimics caloric restriction reducing early mortality in Daphnia.","authors":"A Catherine Pearson, Lev Y Yampolsky","doi":"10.1007/s10522-025-10313-z","DOIUrl":"https://doi.org/10.1007/s10522-025-10313-z","url":null,"abstract":"<p><p>NAD + homeostasis is an important determinant of lifespan and may be a key mechanism of caloric restriction (CR) expansion of lifespan. Ketone bodies such as beta-hydroxybutyrate (BHB) that regulate NAD + abundance and NAD + precursors such nicotinamide mononucleotide (NMN), are known to extend life in experimental animals and ameliorate age-related conditions in humans. We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean. We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet). We show that BHB exposure, but not NMN exposure, reduces early life mortality in fully fed Daphnia to levels similar to those observed under CR without compromising fecundity. We also observed that in a combined exposure cohort, NMN nearly eliminates the beneficial effect of BHB. None of the treatments affected lipofuscin accumulation, but the NMN and the combined treatment mimicked the effect of CR on neonate size in older females. An RNAseq experiment comparing the two diets and the two exposure treatments showed showed that BHB-treated Daphnia change expression of a variety of genes, including genes with known longevity extending effects, but differential expression of few genes is consistent with the effects of CR and their functionality is not clear.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"170"},"PeriodicalIF":4.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jingfang granule extends lifespan and healthspan in Caenorhabditis elegans: insights from RNA-seq analysis of genetic mechanisms.","authors":"Taili Zhao, Yunhua Hou, Chuanjiao Feng, Rui Zhao, Honghua Li, Peipei Zhao, Mengmeng Wang, Jingli Ren, Yiwei Meng, Kuidong Xu, Jia Liu, Xin Yin, Guimin Zhang, Jingchun Yao, Xuekui Xia","doi":"10.1007/s10522-025-10311-1","DOIUrl":"https://doi.org/10.1007/s10522-025-10311-1","url":null,"abstract":"<p><p>Traditional Chinese Medicine (TCM), as a globally recognized phytomedicinal approach, has made significant contributions to healthcare and anti-aging research worldwide. Jingfang Granule (JFG), an established TCM preparation in China, was demonstrated to exert effects on prolonging lifespan and healthspan via the Caenorhabditis elegans model in this study. We employed RNA-seq analysis to investigate the complex genetic interactions through which JFG extends the lifespan of C. elegans. We observed that administering JFG to adult nematodes increased the transcription levels of extracellular matrix (ECM)-related genes (including collagen genes), reproduction-related genes (e.g., egg-1, lin-41), and stress-activated transcription factor-1 (atfs-1). JFG treatment slowed down the functional degradation of organs, such as the cuticle, reproductive system, and mitochondria, leading to enhanced innate immunity and fecundity. Our findings demonstrate that JFG protects nematodes from age-related physiological decline and extends both lifespan and healthspan. This study not only highlights the effects of JFG on delaying aging but also provides a deep understanding of the genetic interactions underlying its anti-aging benefits.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"168"},"PeriodicalIF":4.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of branched chain amino acid metabolism on aging.","authors":"Zhaojia Wang, Shiran Yu, Xiao Du, Xuzhen Yan, Yanguo Xin","doi":"10.1007/s10522-025-10309-9","DOIUrl":"https://doi.org/10.1007/s10522-025-10309-9","url":null,"abstract":"<p><p>Aging is a complex biochemical phenomenon that considerably impacts both individual health and societal dynamics. Recent researches have emphasized the essential function of metabolism in the processes of aging and longevity. Metabolites-chemical byproducts produced by the host organism and its symbiotic partners, including the microbiota, are generated through numerous metabolic pathways. In the last fifteen years, major progress has been made in elucidating the metabolism of BCAAs and the detailed molecular mechanisms that connect BCAAs homeostasis to the aging process. The growing body of literature presents a comprehensive view of the tissue- and disease-specific regulatory mechanisms governing BCAAs and their activation of various molecular pathways. These pathways link fluctuations in BCAA levels to the onset and progression of age-related diseases. This review seeks to consolidate current knowledge on the factors influencing BCAA levels and their metabolic pathways. It further aims to elucidate the molecular mechanisms linking dysregulated BCAA homeostasis to age-related diseases, evaluate epidemiological evidence correlating BCAAs with various cardiovascular conditions, and identify gaps in current understanding that warrant further investigation.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"169"},"PeriodicalIF":4.1,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular and molecular functions of long noncoding RNAs in testis, aging and diseases.","authors":"Ajay Kumar Danga, Pramod C Rath","doi":"10.1007/s10522-025-10312-0","DOIUrl":"https://doi.org/10.1007/s10522-025-10312-0","url":null,"abstract":"<p><p>Reproductive aging is an emerging global health concern, projected to become the third most significant health issue in the near future, according to the World Health Organization. This complex process is driven by molecular and cellular changes, including alterations in DNA, RNA, and protein expression. Among non-coding RNAs (ncRNAs), long non-coding RNAs (lncRNAs) have been increasingly recognized for their regulatory roles in spermatogenesis and their potential contributions to aging and testicular diseases. This review examines the functions of lncRNAs in testicular biology, focusing on their gene-regulatory roles, isoform diversity, subcellular localization, and interactions with key molecular components. While research has historically prioritized protein-coding genes, the extensive ncRNA landscape suggests a broader regulatory network influencing reproductive health. Many testis-specific lncRNAs exhibit conserved sequences, modular structures, and repeat-rich elements, which contribute to their functional significance. Dysregulation of these lncRNAs has been implicated in pathological conditions such as testicular cancer, highlighting their potential as biomarkers and therapeutic targets. Understanding the dynamic roles of lncRNAs in testicular function, aging, and disease is essential for advancing reproductive medicine. This study provides insights into the complex interplay between lncRNAs and reproductive aging, emphasizing their significance in testis-specific processes and associated disorders.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"166"},"PeriodicalIF":4.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing reveals telomere associated genomic differences between healthy and unhealthy aging in a Korean population.","authors":"Ji-Hye Oh, Hyo Jeong Lee, Wonkyung Kim, Da Eun Oh, Hong-Kyu Kim, Eun Hee Kim, Jaewon Choe, Ha Ra Jun, Chae Won Park, Young Gwang Kang, Chong Jai Kim, Chang Ohk Sung, Tae Won Kim","doi":"10.1007/s10522-025-10310-2","DOIUrl":"https://doi.org/10.1007/s10522-025-10310-2","url":null,"abstract":"<p><p>One of the major challenges in modern biogerontology is understanding the accumulation of molecular damage and the manifestation of phenotypic heterogeneity during aging. Notably, genomic instability caused by impaired DNA damage repair along with telomere attrition are primary drivers of aging. However, how these aging-related characteristics differ in individuals who age healthily without developing major age-associated diseases remains unclear. Here, whole genome sequencing (WGS) was performed on 100 healthy agers (≥ 60 years old, no age-related diseases) and 100 unhealthy agers (≥ 60 years old, at least one age-related disease/condition) based on a case-control study. Telomere length was measured using TelSeq and Computel. High-functional impact germline variant (gHFI) burden and alteration pattern at the pathway level were also analyzed. The GTEx dataset including 751 individuals was used to observe the functional impact of identified germline variants at the molecular level. Telomere length showed minimal differences before 65 years of age but declined rapidly in unhealthy agers beyond this age. Additionally, healthy agers had lower gHFI burden, particularly in DNA repair genes such as BLM. Pathway analysis revealed enrichment of oxidative stress-related mutations in healthy agers, correlated with reduced oxidative stress and upregulated antioxidant enzymes (SOD1 and SOD2). Overall, genomic instability preserved through slow telomere attrition and reduced DNA repair defects plays a key role in healthy aging. Improved oxidative stress resistance may contribute to healthier aging, highlighting the role of genetic factors in reducing age-related decline and supporting overall well-being in later life.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"167"},"PeriodicalIF":4.1,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting metabolic regulation of behaviors and physiology during aging in Drosophila.","authors":"Elizabeth S Pasam, Kishore Madamanchi, Girish C Melkani","doi":"10.1007/s10522-025-10306-y","DOIUrl":"10.1007/s10522-025-10306-y","url":null,"abstract":"<p><p>Aging disrupts physiological and behavioral homeostasis, largely driven by one-carbon metabolism, mitochondrial, and metabolic imbalance. To elucidate the roles of conserved metabolic and mitochondrial genes in age-related decline, we employed genetic manipulations in vivo using Drosophila melanogaster models, in a cell-autonomous and non-cell-autonomous manner. By using panneuronal and indirect flight muscle (IFM) specific drivers, we assessed the impact of gene knockdown (KD) or overexpression (OE) on sleep-circadian rhythms, locomotion, and lipid metabolism in a cell-autonomous and non-cell-autonomous manner to address bidirectional neuro-muscle communications. KD of genes such as SdhD and Gnmt leads to a decrease in flight performance, especially in 6 weeks with both drivers. Panneuronal knockdown of genes did not impact the locomotory performance. Whereas knockdown of mAcon1, LSD2, Ampkα, Ald, and Adsl genes showed reduced flight performance, with only IFM-specific driver emphasizing the cell-autonomous role of metabolic genes. Panneuronal KD of Ald, GlyP, mAcon1, and Gnmt genes showed increased total sleep, reduced activity, while Adsl and Ogdh knockdown led to sleep fragmentation, in a mid-age suggests cell-autonomous impact. Functional analysis of AMPK signaling via overexpression and knockdown of Ampkα, as well as expression of the mutant overexpression SNF1A and its kinase-dead mutant, revealed kinase-dependent, age- and tissue-specific modulation of sleep and activity rhythms. Lipid analysis showed that panneuronal overexpression of Ampkα altered lipid droplet number and size in the brain, indicating disrupted lipid homeostasis during aging. These findings on various genes provide us with an understanding of their diverse effects on sleep-activity rhythms, locomotor effects, and communication in cell and non-cell-autonomous roles. Our study emphasizes Ampkα as a central regulator of behavioral and metabolic aging, linking neuronal energy sensing, motor function, and lipid dynamics, and offers mechanistic insights into tissue-specific metabolic regulation with potential relevance for interventions targeting age-related decline and neurodegeneration.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"165"},"PeriodicalIF":4.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cortisol, DHEAS, and the cortisol/DHEAS ratio as predictors of epigenetic age acceleration.","authors":"Rafaela S C Takeshita, Amber T Nguyen, Anthony P Auger, Wilson C J Chung","doi":"10.1007/s10522-025-10307-x","DOIUrl":"10.1007/s10522-025-10307-x","url":null,"abstract":"<p><p>Cortisol has been widely used as biomarker of stress and aging, but confounding effects and disruption of the hypothalamic-pituitary-adrenal axis can lead to misinterpretation of results based on a single measurement. A possible alternative is the co-measurement of cortisol and the adrenal hormone dehydroepiandrosterone-sulfate (DHEAS), a glucocorticoid antagonist that modulates the stress response. Using data from 969 individuals from the Midlife in the United States study, this study aimed to investigate the influence of age, sex, and self-identified biosocial group (SIBG) on DHEAS, cortisol, and the cortisol/DHEAS ratio, to test whether these hormones add predictive power to epigenetic age estimates, and to compare the performance of these three hormonal measures in predicting epigenetic age acceleration (EAA) using sex epigenetic clocks: Horvath, Horvath's skin & blood (Horvath2), Hannum, PhenoAge, GrimAge, and DunedinPACE. Our findings revealed that age, sex and SIBG significantly influenced all three hormonal measures. Controlling for these biodemographic factors, we found that the cortisol/DHEAS was the best predictor of epigenetic clocks. There was a significant and positive correlation between cortisol and Hannum epigenetic age, and between cortisol/DHEAS ratio in three out of the six clocks (Hannum, Horvath2, PhenoAge), but no significant associations between DHEAS and epigenetic age. The cortisol/DHEAS ratio also had a significant and positive correlation with Hannum EAA. DHEAS and cortisol were not significantly associated with EAA for any epigenetic clock. Our results reinforce the importance of co-measuring cortisol and DHEAS in studies investigating the effect of stress in aging processes.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"164"},"PeriodicalIF":4.1,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light phase feeding and estradiol reverse ovariectomy-induced alterations in metabolism and liver clock gene expression in rat.","authors":"Thais S R Cardoso, Nayara A C Horta, Paola Fernandes, Flávia M Araújo, Pedro L Caillaux Luciano, Tito Mafra Cardoso, Lucas R Drummond, Cândido C Coimbra, Maristela O Poletini","doi":"10.1007/s10522-025-10298-9","DOIUrl":"10.1007/s10522-025-10298-9","url":null,"abstract":"<p><p>During aging, the decline in ovarian hormone levels in women is associated with increased weight gain, fat accumulation, and alterations in the circadian timing system. Aligning eating with the activity phase improves metabolic outcomes. In contrast, misalignment entrains the circadian clock in peripheral organs and raises spontaneous locomotor activity (SLA) before mealtime. Given that ovarian estradiol (E2) modulates both metabolism and circadian function, this study aimed to investigate the role of ovariectomy (OVX) on the time-restricted (TR) feeding effects on metabolism. Two-month-old female rats underwent OVX and were fed with TR during either the light or dark phases. TR-DARK feeding did not reverse the weight and fat gain observed in OVX rats under ad libitum (AD) feeding, likely because it did not change the food intake pattern in OVX rats. Conversely, TR-LIGHT reversed the OVX-induced metabolic effects. Next, we test if OVX affects food-entrainment of circadian clocks. TR-LIGHT, regardless of OVX, abolished the peak Per1, Bmal1, Cry2, and Reverb-ɑ expression in the liver. It also increased SLA at food onset independently of OVX. In contrast, OVX elevated liver expression of Per1, Bmal1, and Cry2 at baseline (zeitgeber time, ZT1), and of Reverb-ɑ at peak (ZT6 and ZT13) compared to SHAM-AD rats. To assess the role of E2, OVX rats received a daily injection of E2 at ZT1 for 3 days, and the expression of clock genes was evaluated on the fourth day. In a different group of E2-treated OVX rats, the daily rhythm of SLA was also monitored. E2 treatment reversed the OVX-induced increase in both weight and fat gain, as well as in Per1, Bmal1, and Cry2. However, it did not affect the Reverb-α. E2 promotes an increase in SLA at ZT1 and ZT2. In conclusion, TR-DARK neither alleviates the lack of ovarian hormones in OVX-induced metabolic changes, nor do ovarian hormones participate in food-entrainment of circadian clocks. However, E2 seems to modulate clock gene expression in the liver.</p>","PeriodicalId":8909,"journal":{"name":"Biogerontology","volume":"26 5","pages":"163"},"PeriodicalIF":4.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}