BMC Biotechnology最新文献

{"title":"Optimization of abamectin production by Streptomyces avermitilis and its antagonistic activity against Meloidogyne incognita.","authors":"Mona S Zayed, Ahmed A M Abdelhafez, Ahmed E Mahgoub, Olfat Radwan, Wafaa H Radwan","doi":"10.1186/s12896-026-01112-6","DOIUrl":"10.1186/s12896-026-01112-6","url":null,"abstract":"<p><p>This study aims to evaluate the safety of Streptomyces avermitilis and its extracts, as well as the impact of temperature, pH, and salinity on its growth, nematicidal activity, and abamectin production. It also determines the optimal conditions that promote significant mycelium and abamectin yield. S. avermitilis’ extract was found to be non-toxic to human skin fibroblasts and certain selected microorganisms. The highest mycelium dry weight and nematicidal effectiveness (99%) were achieved at pH 7 after 8 days of incubation at 28 °C, with a weight of 6.45 g/L at 0.1% NaCl and 7.81 g/L at 1% NaCl. The production medium supported significantly the highest mycelium production (23.57 g/L) and abamectin yield (3.23 g/L) as well as the highest specific growth rate (µ = 0.65/d), and multiplication rate (MR) (0.93) and the lowest doubling time td (1.06 d). Taguchi design screened seven variables, and identified potato starch, α-amylase, and incubation period as significant factors (p ≤ 0.05). The Box-Behnken design yielded maximum mycelium (71.08 g/L) and abamectin production (8.30 g/L) under optimal conditions of 150 g/L potato starch, 0.15 g/L α-amylase, and a 10-day incubation period. This investigation demonstrates the strong potential of S. avermitilis as an eco-friendly biocontrol agent against M. incognita and highlights the power of response surface methodology in optimizing secondary metabolite production for commercial and agricultural applications.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel therapeutic targets for ovarian hypofunction through hormonal pathway regulation.","authors":"Yunqi Wang, Lijuan Xiang, Rui Zhao, Xiao-Juan Zhang, Yang Chen","doi":"10.1186/s12896-026-01110-8","DOIUrl":"10.1186/s12896-026-01110-8","url":null,"abstract":"<p><p>Ovarian hypofunction (OH) is a multifactorial endocrine disorder characterized by impaired folliculogenesis, hypoestrogenism, and subfertility, affecting an estimated 10–12% of reproductive-aged women worldwide and up to 1% of women under 40 years of age. Current interventions, including hormone replacement therapy (HRT), gonadotropin stimulation, and assisted reproductive technologies (ART), remain largely symptomatic, carry safety concerns, and fail to restore intrinsic ovarian activity. To address this unmet need, we employed an integrative computational strategy combining systems-level biological insights with immunoinformatics approaches to identify and evaluate a multi-epitope peptide candidate targeting the follicle-stimulating hormone receptor (FSHR). Seven FSHR-derived peptides with high predicted immunogenic potential were identified, validated for non-allergenicity and non-toxicity, and fused with flexible linkers into a 62-amino-acid construct. Physicochemical analysis indicated stability (instability index 38.80), thermostability (aliphatic index 89.68), hydrophilicity (GRAVY −0.365), and favorable solubility (Protein-Sol 0.584 compared with an E. coli average of 0.45). Codon optimization improved the codon adaptation index from 0.64 to 0.90 and reduced GC content from 63.98% to 53.23%, suggesting high expression potential in E. coli. Molecular docking with TLR2 yielded a favorable docking score (−296), supported by extensive hydrogen bonding and hydrophobic interactions. Long-timescale molecular dynamics simulations (500 ns) supported structural stability, with RMSD plateauing at approximately 0.3 nm, a compact radius of gyration (2.52–2.55 nm), and a persistent hydrogen-bond network (1250–1400 bonds). In silico immune simulations suggested balanced humoral and cellular immune responses, characterized by early IgM production, class switching to IgG, IL-2–associated T-cell expansion, and immune memory formation. Overall, this computational study identifies a structurally stable and immunologically promising peptide candidate for ovarian hypofunction, providing a rational basis for future experimental validation.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical and biological properties of trabecula and strut scaffolds in bone defect reconstruction.","authors":"Linlin Liu, Shuxian Wang, Xin Wang, Juncai Liu","doi":"10.1186/s12896-026-01126-0","DOIUrl":"10.1186/s12896-026-01126-0","url":null,"abstract":"<p><p>BACKGROUND: Different porous structures have different mechanical and biological properties in bone defect repair, and different types of porous structures should be applied in different scenarios. In this paper, trabecula scaffolds and strut scaffolds under different porosity conditions were designed and fabricated by 3D printing. Their mechanical and biological properties were studied, and their fluid characteristics were analyzed using computational fluid dynamics (CFD). RESULTS: The results showed that the yield strength of the strut scaffold was higher than that of the trabecula scaffold, and the strut scaffold was subjected to uniform force, with the maximum stress being much smaller than that of the trabecula scaffold under the same force. The bone ingrowth performance of the trabecula structure scaffold was better than that of the strut scaffold. CFD analysis indicated that the trabecula scaffold had more fluid flow areas inside and provided greater mechanical stimulation to the pillars, which was conducive to bone tissue growth. CONCLUSION: The study demonstrated that different types of porous structures could be selected based on the characteristics of different implantation sites when designing porous scaffolds, thereby maximizing the advantages of 3D printed porous scaffolds.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13069813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial bioleaching of rare earth elements from phosphate minerals: a biotechnology-driven systematic review of mechanisms, bioprocess determinants, and opportunities for sustainable recovery.","authors":"Sonali Prabodha Vijayarathna, Ileperumaarachchige Vayanga Nishani Rathnayake, Pradeep Wishwanath Samarasekere","doi":"10.1186/s12896-026-01125-1","DOIUrl":"10.1186/s12896-026-01125-1","url":null,"abstract":"<p><strong>Background: </strong>Rising demand for rare earth elements (REEs) and the severe environmental impact of conventional extraction from phosphate minerals (monazite, apatite) have intensified the search for green alternatives. Microbial bioleaching offers a low-energy, low-waste, and a sustainable biotechnological alternative by exploiting the ability of fungi and bacteria to generate organic acids, siderophores, reducing agents, and other metabolites that solubilize REEs. Although interest in REE bioleaching has increased, a biotechnology-focused synthesis of microbial mechanisms, metabolic constraints, and process determinants specific to phosphate matrices remains limited.</p><p><strong>Methods: </strong>A PRISMA-guided systematic review was conducted. Scopus, Web of Science, PubMed, and Google Scholar and other major databases were searched to identify peer-reviewed studies reporting microbial bioleaching of REEs from phosphate minerals. From 443 identified records, 25 studies met the inclusion criteria after screening and eligibility assessment. These studies were evaluated based on microbial species, metabolic mechanisms, culture conditions, mineral substrates, and REE solubilization performance.</p><p><strong>Results: </strong>Fungal species, particularly Aspergillus, Penicillium and Paecilomyces demonstrated the highest REE mobilization efficiencies through intensive production of citric, oxalic, and gluconic acids, along with phosphatase activity. Bacterial strains, including Acidithiobacillus, Bacillus, Pantoea, Burkholderia, Pseudomonas, and Klebsiella contributed complementary mechanisms such as proton extrusion, siderophore secretion, and Fe(III) / Fe(II) redox cycling. Bioleaching performance was strongly influenced by media composition, carbon source, nitrogen assimilation, pH evolution, mineralogy of the phosphate substrate, pulp density, and particle size. Across studies, the lack of standardized conditions limited direct comparability, but organic acid dominated pathways consistently produced the most robust REE solubilization.</p><p><strong>Conclusions: </strong>Microbial bioleaching is a promising biotechnological platform for REE recovery from phosphate minerals, driven by metabolically diverse acidogenic, chelating, enzymatic, and redox mechanisms. However, advancements remain constrained by heterogeneous methodologies, limited integration of mechanistic studies, and minimal use of engineered strains or controlled bioreactor systems. Future progress requires standardized experimental frameworks, improved mechanistic understanding of organism-specific roles, and rational design of optimized microbial systems. This review offers a biotechnology-centered foundation to guide next-generation research on sustainable REE mobilization from phosphate resources.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles co-loaded with bacillus Calmette-Guérin pure protein derivative and doxorubicin for bladder tumor therapy.","authors":"Jindong Zhang, Shuai Su, Maoyu Liu, Yu Luo, Chengcheng Wei, Yang Cao, Honglin Cheng, Shenyin Zhu, Delin Wang","doi":"10.1186/s12896-026-01122-4","DOIUrl":"10.1186/s12896-026-01122-4","url":null,"abstract":"<p><p>Bladder perfusion chemotherapy remains the standard treatment for bladder cancer, yet its effectiveness is frequently limited by rapid drug clearance through urinary excretion and inadequate infiltration of immune cells into bladder tissue. To address these challenges, we developed an active-targeting nano-drug delivery system specifically designed for bladder tumors. This system utilizes a sialic acid-targeted poly (lactic-co-glycolic acid) (PLGA) platform to co-deliver doxorubicin (DOX) and the purified protein derivative (PPD) of bacillus Calmette-Guérin (BCG-PPD). By leveraging the selective binding of phenylboronic acid to sialic acid, the system enhances tumor-specific drug uptake, significantly amplifying DOX’s therapeutic efficacy and inducing immunogenic cell death. Furthermore, BCG-PPD exerts potent immunostimulatory effects, promoting dendritic cell (DC)-mediated tumor antigen processing and presentation, which in turn drives robust cytotoxic T lymphocyte (CTL) infiltration into the tumor microenvironment. The superior anti-tumor performance of this system was validated in an orthotopic bladder cancer mouse model. In conclusion, by synergistically combining targeted drug delivery with chemo-immunotherapy, our nanoparticle system presents a highly effective and promising new paradigm for bladder cancer therapy.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147321253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-synthesized Desertifilum silver nanoparticles induce BAX/BCL-2 mediated apoptosis in Ehrlich carcinoma.","authors":"Amira L Hanna, Atef S Elgebaly, Magdy M Mohmed, Mahmoud W Sadik, Hayam M Hamouda, Hanan A Goda, Afaf Altrawy","doi":"10.1186/s12896-026-01107-3","DOIUrl":"10.1186/s12896-026-01107-3","url":null,"abstract":"<p><p>BACKGROUND: Green and biogenic silver nanoparticles (AgNPs) are widely researched as anticancer treatments; nevertheless, in vivo data on cyanobacteria-derived formulations and their apoptotic mechanisms are limited. OBJECTIVES: To assess the antitumor activity, hematological safety, and apoptosis-related pathways of Desertifilum tharense-derived AgNPs, provided either independently or in conjunction with Desertifilum exopolysaccharides (EPS), in a mouse Ehrlich cell carcinoma (ECC) model. METHODS: Male Swiss albino mice were assigned to five groups: healthy control, AgNP toxicity control, tumor control, AgNP-treated tumor, and AgNPs + EPS-treated tumor. Endpoints encompassed body weight, thigh tumor volume, hematological profiling, histology, immunohistochemistry identification of cleaved caspase 3, and real-time PCR quantification of BAX, BCL-2, and caspase 3 mRNA, utilizing the BAX/BCL-2 ratio as an indicator of apoptotic equilibrium. RESULTS: Tumor control subjects exhibited significant increases in body weight and tumor volume, along with anemia, leukocytosis, and altered leukocyte differentials. The AgNP-treated groups had markedly decreased tumor sizes and partial restoration of hematological parameters, whereas healthy mice treated solely with AgNP maintained values within physiological limits. Tumor sections from treated mice exhibited significant necrosis and a treatment-dependent increase in cleaved caspase 3 staining, most apparent and widespread in the AgNPs + EPS group. Gene expression analysis demonstrated a considerable upregulation of BAX and caspase 3, alongside a downregulation of BCL-2 in treated tumors, with the combination formulation exhibiting the highest BAX/BCL-2 ratio, indicative of pronounced activation of mitochondria-mediated apoptosis. CONCLUSIONS: Desertifilum-derived AgNPs demonstrate in vivo antitumor efficacy against ECC with satisfactory hematological safety and induce caspase 3 dependent, mitochondria-mediated apoptosis via regulation of the BAX/BCL-2 axis. The co-administration with EPS enhances the pro-apoptotic gene and protein profile, endorsing cyanobacteria-based AgNP/EPS systems as viable candidates for future nano-based cancer therapies.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12951942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147301629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Rumex. crispus seeds and synergistic application of photodynamic/photothermal antimicrobial therapy.","authors":"Atiyeh Nomani, MohammadReza Aghdaee, Sajad Shariat, Naser Faraji, Siamak Javani","doi":"10.1186/s12896-026-01120-6","DOIUrl":"10.1186/s12896-026-01120-6","url":null,"abstract":"<p><p>BACKGROUND: Antibiotic resistance and the emergence of antimicrobial resistance (AMR) threaten global health. It is necessary to explore new solutions, such as the use of metal-based nanoparticles and nanomaterials for antimicrobial photodynamic/photothermal therapy (aPDT/aPTT). In this paper, we investigated the antibacterial effect, PTT, and PDT performance of biogenic silver nanoparticles (AgNPs) and nanostructures derived from them. METHOD: Biogenic AgNPs (R. crispus seeds mediated AgNPs, RC-AgNPs) are produced by the extract of the R. crispus seeds. The morphology of RC-AgNPs-treated bacteria was studied using scanning electron microscopy (SEM). Then RC-AgNP @ poly-G was synthesized by the interaction of poly-G with biogenic AgNPs. Nanostructures characterized by UV-visible spectroscopy, Fourier-transform infrared (FT-IR), dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The entropy and temperature studies of poly G secondary structure were carried out using the Vienna RNA secondary structure server and UV-visible spectroscopy at 25°, 37°, and 40° C. RESULTS: Results have shown that RC-AgNPs and RC-AgNP @ poly-G exhibit a synergistic antibacterial effect with PTT and PDT. CONCLUSIONS: In summary, these nanoparticles could be nanotheranostic agents. They have acceptable potential in the therapy and diagnosis of infectious and non-infectious diseases. Consequently, they are good objectives for future research.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13040958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLGA nanofibers carrying rhFGF1 for the effective treatment of skin wounds in diabetic mice.","authors":"Yujie Zhang, Xianshi Wang, Shasha Ye, Jiana Li, Junyi Wu, Xiaoyan Bao, Yeli Zhao","doi":"10.1186/s12896-026-01121-5","DOIUrl":"10.1186/s12896-026-01121-5","url":null,"abstract":"<p><p>Diabetic foot ulcers are among the most common complications of diabetes and can lead to delayed wound healing. Fibroblast growth factor (FGF1) is a classic drug for the treatment of skin wounds but has the disadvantages of a short half-life and instability. Poly (lactic-co-glycolic acid) (PLGA) nanofibers are sustained-release biomaterials that have potential for use as therapeutic delivery systems. However, the therapeutic effect of PLGA loaded with recombinant human FGF1 (rhFGF1) on diabetic wound healing is unknown. Therefore, this study aimed to explore the therapeutic effects of PLGA-rhFGF1 in type 2 diabetic (T2D) wound mice. We found that PLGA offers good sustained release, which enhances the stability and bioactivity of rhFGF1. PLGA-rhFGF1 promoted wound closure, re-epithelialization and the expression of keratin 10 and keratin 14 in T2D mice on day 14. PLGA-rhFGF1 significantly decreased the levels of TNF-α and IL-6 in serum and skin tissues as well as the level of IL-1β in skin tissues. Moreover, PLGA-rhFGF1 decreased the mRNA levels of CXCL1, MCP1 and MIP2, and the fluorescence intensity of F4/80 and Ly6G in T2D wound mice and increased the collagen content and the protein expression of collagen I in the dermis of T2D wounds. PLGA-rhFGF1 also increased blood flow; the mRNA levels of the angiogenesis-related factors VEGF, Ang-1 and eNOS and the fluorescence intensity of CD31 in T2D wound mice. These data indicate that PLGA releases rhFGF1 slowly and promotes skin wound healing in T2D mice. The mechanisms through which PLGA-rhFGF1 produces these effects involve decreased inflammation and the promotion of granulation, re-epithelialization and angiogenesis.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13040920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147301600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin potentiates DSF/Cu-loaded pluronic nanoparticles to improve therapeutic efficacy in triple-negative breast cancer.","authors":"Mehdi Pourbakhsh, Abolfazl Doustmihan, Masoud Jabraili, Marziyeh Fathi, Rana Jahanban Esfahlan","doi":"10.1186/s12896-026-01113-5","DOIUrl":"10.1186/s12896-026-01113-5","url":null,"abstract":"<p><p>BACKGROUND: Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype characterized by poor prognosis and resistance to conventional therapies, underscoring the need for novel therapeutic strategies. Drug repurposing has emerged as a promising approach, with disulfiram (DSF) and metformin demonstrating anticancer potential. In addition, nanoparticle (NP)-based drug delivery systems can enhance therapeutic efficacy and tumor selectivity. This study investigates the antitumor effects of a combination therapy using DSF/Cu complex–loaded pluronic-based nanoparticles and metformin in TNBC cells. METHODS: Pluronic-based nanoparticles were synthesized using the thin-film hydration method, and hyaluronic acid (HA) was conjugated to pluronic, as confirmed by H-NMR spectroscopy. Drug–nanoparticle interactions were characterized using FTIR analysis. Drug loading efficiency, release profiles, and cellular uptake were evaluated under acidic and physiological conditions. Cytotoxic effects of free drugs and NP formulations were assessed using the MTT assay, while apoptosis was analyzed by flow cytometry. The expression levels of STAT3, CD24, CD44 and ALDH1 were quantified by real-time PCR. RESULTS: Successful conjugation of HA to pluronic was confirmed by H-NMR, and FTIR analysis verified physical interactions between the drugs and pluronic micelles. Encapsulation efficiencies for DSF and DSF/Cu were 89.5% and 73%, respectively. DSF/Cu-loaded nanoparticles exhibited a faster drug release under acidic conditions compared to physiological pH. NP formulations significantly enhanced cellular uptake and demonstrated a synergistic-like potentiating effect, wherein sub-cytotoxic concentrations of metformin markedly enhanced the anticancer efficacy of DSF/Cu-loaded nanoparticles. Apoptosis assays revealed increased apoptotic cell death with minimal necrosis. Furthermore, real-time PCR analysis showed significant downregulation of STAT3 and stemness-associated gene expression compared to untreated controls. CONCLUSION: Pluronic-based nanoparticles efficiently encapsulate DSF/Cu and enhance its anticancer activity. The combination of metformin with DSF/Cu-loaded nanoparticles significantly inhibits TNBC cell growth and reduces stemness properties. This low-toxicity, metabolism-assisted combination strategy shows strong potential for TNBC treatment and warrants further in vivo investigation.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13032520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and evaluation of hydrogel based on Brazilian propolis ethanol extract for promoting diabetic wound healing.","authors":"Huanglin Huo, Zhenhua Zhou, Rongxiang Guo, Jinyi Zhang, Menghan Li, Huicong Zeng, Dongyu Zhao, Roman P Terekhov, Peng Jiang, Qian Zhou, Bo Li","doi":"10.1186/s12896-026-01115-3","DOIUrl":"10.1186/s12896-026-01115-3","url":null,"abstract":"<p><p>Diabetes wounds represent a significant clinical challenge, primarily attributed to their complex microenvironment characterized by persistent inflammation, heightened infection susceptibility, and impaired angiogenesis. To address these interconnected challenges, a multifunctional hydrogel dressing (designated EEP/PS) was constructed using pullulan, Brazilian propolis ethanolic extract, and polyvinyl alcohol. This biomaterial exhibits an interconnected porous structure, suitable mechanical properties, tissue adhesion, remarkable thermal stability, excellent sustained-release and swelling capacity. Notably, EEP/PS shows potent antioxidant capability and significantly enhances cell migration in hydrogen peroxide-induced oxidative injury models. Furthermore, the hydrogel downregulates the expression and secretion of key pro-inflammatory cytokines in LPS-stimulated macrophages, including tumor necrosis factor-α, interleukin-6, and interleu-kin-1β. The hydrogel also exhibits broad-spectrum antibacterial efficacy against common wound pathogens Escherichia coli and Staphylococcus aureus. In a diabetic rat model, EEP/PS accelerated wound closure by synergistically promoting reepithelialization, collagen deposition, angiogenesis while mitigating local inflammation. Additionally, EEP/PS exhibits an excellent hemostasis capacity and bio-compatibility, critical for clinical translation. Collectively, these findings highlight the translational potential of EEP/PS as a multifunctional biomaterial for diabetic wound care.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13032371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146775976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}