BMC Biotechnology最新文献

{"title":"Electrospun PCL membranes for localized drug delivery and bone regeneration.","authors":"Betul Gedik, Mehmet Ali Erdem","doi":"10.1186/s12896-025-00965-7","DOIUrl":"https://doi.org/10.1186/s12896-025-00965-7","url":null,"abstract":"<p><strong>Background: </strong>Bone loss caused by cysts, tumors, trauma, and other factors is a significant challenge in medicine and dentistry. Effective bone regeneration is essential for accelerated healing and improved bone volume. While systemic drug supplementation helps, local delivery through gbr/gtr membranes is preferred for targeted treatment with minimal systemic effects. This study aims to develop drug-loaded gbr membranes using electrospinning to enhance localized drug delivery and tissue regeneration.</p><p><strong>Methods: </strong>Polycaprolactone (PCL) membranes were produced via electrospinning with various concentrations and solvent ratios. Therapeutic agents-pentoxifylline, carrageenan, and sodium fluoride-were incorporated into the membranes. Morphological analysis was performed using scanning electron microscopy (SEM), mechanical properties were assessed through tensile testing, structural characterization was done via Fourier-transform infrared spectroscopy (FTIR), and thermal properties were evaluated with thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Drug release behavior was studied using UV-Vis spectrophotometry.</p><p><strong>Results: </strong>SEM revealed optimal fiber morphology in membranes with 10% PCL and 1% pentoxifylline, 0.5% NaF, and 1% carrageenan. Tensile strength was highest in 10% PCL membranes (2.5 MPa), outperforming 12% PCL (1.8 MPa). FTIR and TGA confirmed successful drug incorporation and thermal stability, with decomposition temperatures ranging from 395 °C to 510.9 °C. UV-Vis showed effective drug release, with 2% pentoxifylline achieving the highest release at 2 h (34%) and 4 h (62%), demonstrating enhanced performance for localized drug delivery.</p><p><strong>Conclusions: </strong>PCL-based electrospun membranes with therapeutic agents were successfully developed, exhibiting promising characteristics for localized drug delivery and tissue regeneration. These membranes showed comparable mechanical properties to commercial GBR/GTR membranes. Future research should focus on optimizing formulations and evaluating clinical efficacy.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"31"},"PeriodicalIF":3.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12023437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted cancer therapy potential of quercetin-conjugated with folic acid-modified nanocrystalline cellulose nanoparticles: a study on AGS and A2780 cell lines.","authors":"Mozhgan Soltani, Negar Ahmadzadeh, Hasti Nasiraei Haghighi, Niloufar Khatamian, Masoud Homayouni Tabrizi","doi":"10.1186/s12896-025-00962-w","DOIUrl":"https://doi.org/10.1186/s12896-025-00962-w","url":null,"abstract":"<p><p>This study investigates the effects of quercetin-conjugated nanocrystalline cellulose/cetyltrimethylammonium bromide/folic acid nanoparticles (NCC/CTAB/FA NPs) on AGS and A2780 cancer cell lines, focusing on their cytotoxicity and antioxidant capacity. Dynamic light scattering (DLS) analysis revealed an average particle size of 388.70 nm, suitable for cellular uptake and release kinetics. The NCC/CTAB/FA NPs exhibited a rod and spherical morphology and uniform distribution, as confirmed by field emission scanning electron microscopy (FESEM). Fourier-transform infrared (FTIR) spectroscopy confirmed the successful synthesis and functional group integration, supporting the NPs' ability for drug delivery. The encapsulation efficiency value was 81.17%, demonstrating the effective incorporation of Quercetin. Cytotoxicity assays indicated significant reductions in cell viability for AGS and A2780 cells with IC₅₀ values of 3.2 µg/mL and 16.04 µg/mL, respectively, while HDF cells exhibited higher viability. Flow cytometry analysis revealed a dose-dependent induction of apoptosis in AGS cells, supported by changes in gene expression related to apoptosis and inflammation. Furthermore, antioxidant capacity assays demonstrated practical free radical scavenging abilities, with IC₅₀ values of 151.65 µg/mL for ABTS and 349.54 µg/mL for DPPH. NCC/CTAB/FA/Quercetin NPs exhibit promising characteristics for targeted cancer therapy and antioxidant applications.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"29"},"PeriodicalIF":3.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12001405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parabacteroides distasonis promotes CXCL9 secretion of tumor-associated macrophages and enhances CD8⁺T cell activity to trigger anti-tumor immunity against anti-PD-1 treatment in non-small cell lung cancer mice.","authors":"Zhijun Fan, Zheng Yi, Sheng Li, Junjun He","doi":"10.1186/s12896-025-00963-9","DOIUrl":"https://doi.org/10.1186/s12896-025-00963-9","url":null,"abstract":"<p><strong>Background: </strong>Parabacteroides distasonis (P. distasonis) could regulate inflammatory markers, promote intestinal barrier integrity, and block tumor formation in colon. However, the regulatory effect of P. distasonis on non-small cell lung cancer (NSCLC) remains unknown. This study aimed to investigate the regulatory effect of P. distasonis on NSCLC and its impact on tumor immunity.</p><p><strong>Methods: </strong>We first established a mouse model of Lewis lung cancer, and administered P. distasonis and intrabitoneal injection of anti-mouse PD-1 monoclonal antibody to assess the impact of P. distasonis on tumor immunity, and mouse intestinal barrier. Then, we explored the effect of P. distasonis on CD8⁺T cells and CXCL9 secretion mediated by tumor-associated macrophages (TAM). We used the TLR1/2 complex inhibitor CPT22 to evaluate its effect on macrophage activation. Finally, we explored the effect of P. distasonis on CD8⁺T cells and CXCL9 secreted by TAM in vivo.</p><p><strong>Results: </strong>In vivo, P. distasonis enhanced anti-tumor effects of anti-PD-1 in NSCLC mice, improved intestinal barrier integrity, recruited macrophages, and promoted M1 polarization. In vitro, CD86 and iNOS levels in BMDM were elevated and CD206 and Arg1 levels were suppressed in membrane fraction of P. distasonis (PdMb) group in comparison to Control group. With additional CPT22 pre-treatment, the levels of CD86 and iNOS in BMDM were reduced, and the levels of CD206 and Arg1 were increased. Compared to PBS group, P. distasonis group exhibited higher proportion of CD8⁺T cells in tumor tissues, along with increased positive proportion of GZMB and IFN-γ in CD8⁺T cells. Additionally, in comparison to Control group, PdMb group showed an elevated proportion of GZMB⁺T and IFN-γ⁺T cells within CD8⁺T cells, and secretion of IFN-γ, TNF-α, perforin, and GZMB in CD8⁺T cell supernatant increased. Moreover, the proportion of CXCL9⁺F4/80⁺ macrophages in tumor tissues was higher in P. distasonis group compared to PBS group. In comparison to Control group, CXCL9 protein level in BMDM and CXCL9 secretion level in BMDM supernatant were increased in PdMb group. Finally, P. distasonis enhanced CD8⁺T cell activity by secreting CXCL9 from macrophages in vivo.</p><p><strong>Conclusions: </strong>P. distasonis promoted CXCL9 secretion of TAM and enhanced CD8⁺T cell activity to trigger anti-tumor immunity against anti-PD-1 treatment in NSCLC mice.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"30"},"PeriodicalIF":3.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Keratinase from Pseudomonas aeruginosa-C1M, characterization and applications as novel multifunctional biocatalyst.","authors":"Marium Saba, Safia Farooq, Abdulrahman H Alessa, Kadriye Inan Bektas, Ali Osman Belduz, Alam Zeb Khan, Aamer Ali Shah, Malik Badshah, Samiullah Khan","doi":"10.1186/s12896-025-00959-5","DOIUrl":"https://doi.org/10.1186/s12896-025-00959-5","url":null,"abstract":"<p><strong>Introduction: </strong>This study explores the biogenic synthesis of silver nanoparticles (AgNPs) using keratinase from Pseudomonas aeruginosa-C1M as a reducing and stabilizing agent. The synthesis of AgNPs was characterized by a color change from transparent to dark brown and a UV-Vis absorption peak at 450 nm, confirming nanoparticle formation. The study further investigates the structural, morphological, and functional properties of these AgNPs, particularly their antibacterial activity and their potential role in azo dye decontamination.</p><p><strong>Methods and results: </strong>The FTIR confirmed that AgNPs nanoparticles formation with keratinase. X-ray diffraction analysis showed that the prepared AgNPs were crystalline in nature and had face-centered cubic lattice planes. When observed under the transmission electron microscope and scanning electron microscope the nanoparticles were monodispersed spheres of different sizes. The diameter of the AgNPs was ~ 119 nm according to dynamic light scattering. High dispersion, long-term stability and excellent colloidal properties were supported by a high negative zeta potential value. The silver nanoparticles were found to have an antibacterial activity with zone of inhibition 25 mm and 33 mm against pathogenic strains of Staphylococcus aureus and Escherichia coli respectively. The synthesized zero-valent silver nanoparticles assisted in the decontamination of azo dyes (methyl red, methyl orange, safranin O and methyl violet) through the incorporation of sodium borohydride and light-catalyzed processes.</p><p><strong>Conclusion: </strong>This study demonstrates, for the first time, that keratinase from Pseudomonas aeruginosa-C1M can be used for AgNPs synthesis. The biogenic AgNPs exhibited potent antibacterial activity and played a crucial role in detoxifying hazardous azo dyes. These findings highlight the dual-functional potential of AgNPs for applications in antimicrobial treatments and environmental remediation. Future studies should explore their mechanism of action, scalability, and industrial applications.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"27"},"PeriodicalIF":3.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells.","authors":"Sandra Majo, Chloe Redoute-Timonnier, Aurelie Lacour, Laurine Challeat, Eva Epinette, Jeremie Teillon, Christophe F Grosset, Patrick Auguste","doi":"10.1186/s12896-025-00961-x","DOIUrl":"https://doi.org/10.1186/s12896-025-00961-x","url":null,"abstract":"<p><p>Three-dimensional spheroids are more representative of tumors than cell-cultured monolayers. As in tumors, gradients of oxygen, nutrients and wastes are found in spheroid cultures but not in classical cultured monolayers. On the other hand, cell-based assays on the latter are hardly applicable to spheroid cultures. Such is the case for zymogram assays, which are classically used to measure MMP-2 and MMP-9 activities, and for immunoblots to measure the phosphorylation of proteins involved in ligand-induced intracellular signaling in normal and tumor cells. In this study we used two renal cancer cell lines as models, the first derived from a pediatric rhabdoid tumor and the second from an adult clear cell renal cell carcinoma. Using these two cell lines, we successfully developed a simple inexpensive assay to measure MMP-2 and MMP-9 activities in spheroids established in the presence of methylcellulose. After washing, 1 to 5 spheroids were pooled and stimulated with collagen I for 24 h before analysis. MMP-2 and MMP-9 activities were measured in supernatants using a standard but enhanced zymogram assay. Both pro-MMP-9 and MMP-2 activities were detected in spheroids established from both cell lines. In contrast with our previous data using classical cultures monolayers, collagen I stimulation decreased pro-MMP-9 activity without affecting MMP-2 activity. On the other hand, we could not accurately measure AKT intracellular signaling pathways from spheroids stimulated with collagen I. Finally, we adapted our 3D protocol to analyze the MAPK/ERK pathway in kidney tumor cells following induction by EGF. In conclusion, this zymogram assay for analyzing MMP-2 and MMP-9 activities in spheroids paves the way for novel experimentations in tumor biology.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"28"},"PeriodicalIF":3.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of loading thymoquinone to gold or silver nanoparticles on the efficacy of anti-tumor treatments in breast cancer with or without chemotherapeutic cisplatin.","authors":"Soha Gomaa, Mohamed Nassef, Ahlam Abu-Shafey, Mona Elwan, Asmaa Adwey","doi":"10.1186/s12896-025-00958-6","DOIUrl":"https://doi.org/10.1186/s12896-025-00958-6","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Nanotechnology has been greatly examined for tumor medication, as nanoparticles (NPs) serve a crucial role in drug delivery mechanisms for cancer therapy. In contrast to traditional cancer therapies, NPs-based drug delivery offers several benefits, including increased stability and biocompatibility, improved retention capabilities and permeability, as well as precise targeting.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;The objective of this study was to examine the tumor-targeting efficacy of Thymoquinone (TQ)-loaded gold NPs (AuNPs/TQ conjugate) or TQ-loaded silver NPs (AgNPs/TQ conjugate) in conjunction with the conventional chemotherapy agent cisplatin (CP) in Ehrlich ascites carcinoma (EAC)-bearing mice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The loading capacity of synthesized conjugates was characterized by UV-Vis spectra and transmission electron microscope (TEM). We used CD-1 mice with a peritoneal EAC tumor xenograft model that received oral administration of TQ, AuNPs, AgNPs, AuNPs/TQ conjugate, and AgNPs/TQ conjugate.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;EAC-bearing mice received daily oral administration of one of the following treatments for six consecutive days: TQ, AuNPs, AgNPs, AuNPs/TQ, AgNPs/TQ, AuNPs/TQ + CP, or AgNPs/TQ + CP conjugates. Eleven days after EAC inoculations, assessments were conducted to evaluate the total number of tumor cells, splenocytes, white blood cells (WBCs), C-reactive protein (CRP) levels, flow cytometric analysis of apoptosis in EAC cells, as well as the functionality of the kidney and liver.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;EAC-bearing mice that received treatment with TQ, AuNPs, AgNPs, AuNPs/TQ, and AgNPs/TQ exhibited significantly enhanced anti-tumor activity and improved therapeutic efficacy. Our results further revealed that the combined synergistic approach of TQ's anti-tumor properties, along with the efficient penetration abilities of AuNPs or AgNPs, led to a significant inhibition of the growth of tumor cells in EAC tumor-bearing mice. Moreover, the incorporation of CP into the AuNPs/TQ or AgNPs/TQ conjugates substantially augmented the anti-proliferative effects against EAC tumor cells, effectively overcoming resistance to chemotherapeutic agents. Furthermore, our data revealed that this combination resulted in an elevation of leukocyte counts, along with an increase in the absolute quantities of lymphocytes, neutrophils, and monocytes, thereby activating the immune system and reducing the inflammatory marker CRP. However, the restoration of splenocyte levels, which had been reduced due to EAC cell inoculation, required an extended period to return to baseline. Furthermore, the results indicated moderate alterations in the functionality of both the liver and kidney.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;To conclude, AuNPs, AgNPs, AuNPs/TQ, and AgNPs/TQ may hold great promise as potential nanoparticle-based therapies for cancer treatment. Additionally, provides numerous benefits compared to ","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"26"},"PeriodicalIF":3.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques.","authors":"Mustafa Sevindik, Celal Bal, Tetiana Krupodorova, Ayşenur Gürgen, Emre Cem Eraslan","doi":"10.1186/s12896-025-00960-y","DOIUrl":"10.1186/s12896-025-00960-y","url":null,"abstract":"<p><p>In this study, the biological activities of Otidea onotica were investigated using two optimization methods, Response Surface Methodology (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA). The extracts were tested for phenolic content, antioxidant potential, acetylcholinesterase and butyrylcholinesterase inhibitory activities and antiproliferative effects against A549 lung cancer cell line. The results show that the extracts obtained by ANN-GA optimization exhibited higher antioxidant activity compared to RSM extracts and had higher total antioxidant status (TAS), DPPH and FRAP values. Phenolic content analysis revealed eight phenolic compounds and the compounds with the highest concentrations were caffeic acid (in RSM extract) and gallic acid (in ANN-GA extract), respectively. Both extracts showed strong cytotoxic effects against A549 cells depending on the concentration, with ANN-GA extract showing higher antiproliferative activity. Our study provides important findings on the biological activities and therapeutic potential of O. onotica and particularly reveals that the ANN-GA optimization method plays an important role in increasing biological activity. The findings show that O. onotica extracts can be used in the treatment of cancer and neurodegenerative diseases in the future and that optimization techniques offer an effective strategy for enriching phenolic contents.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"25"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro.","authors":"Oksana Mykchaylova, Aleksander Besarab, Anatoliy Negriyko, Margarita Lomberg, Natalia Poyedinok","doi":"10.1186/s12896-025-00957-7","DOIUrl":"10.1186/s12896-025-00957-7","url":null,"abstract":"<p><strong>Background: </strong>The problem of searching for environmentally friendly regulators of the biosynthetic activity of edible and medicinal mushrooms is crucial for creating highly effective biotechnologies. One such regulator is light. This study aimed to compare and evaluate the fatty acid profile and fat quality indices of lipids from the mycelial mass of Pleurotus eryngii under various light-emitting diode (LED) and laser light irradiation regimes.</p><p><strong>Methods: </strong>To determine the effect of artificial light on the biosynthetic activity of P. eryngii, an artificial lighting system based on LED matrices with wavelengths of 470 nm (blue), 530 nm (green), and 650 nm (red), as well as an argon gas laser as a coherent visible light source at 488 nm, was used. For all experimental variants, the energy density on the surface of the samples was set to the same, providing an energy dose of 240 mJ/cm². Irradiation was carried continuously.</p><p><strong>Results: </strong>Twenty-seven fatty acids were identified in the studied P. eryngii mycelial mass samples, including nine saturated fatty acids (SFAs), eight monounsaturated fatty acids (MUFAs) and ten polyunsaturated fatty acids (PUFAs). The control sample (without irradiation) contained the lowest number of fatty acids (fourteen). With irradiation in all modes, a decrease in the amount of SFA and the formation of new MUFA and PUFA with a chain length of C20-C24, which were absent in the control, were observed. Blue light stimulated the synthesis of significant amounts of α-linolenic acid (C18:3ω-3). The ratios of ΣPUFA/ΣSFA, ΣPUFA/ΣMUFA and ΣPUFAω-6/ω-3 in the mass of mycelium irradiated with blue light were within the optimal values for the human diet.</p><p><strong>Conclusions: </strong>The selected mycelial photoactivation modes using low-intensity laser and LED light of different spectral composition and coherence may have potential in the biotechnology of submerged cultivation of P. eryngii to obtain mycelial mass with an improved fatty acid profile, which can be considered as a useful source of lipids.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"24"},"PeriodicalIF":3.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Neural Network - Multi-Objective Genetic Algorithm based optimization for the enhanced pigment accumulation in Synechocystis sp. PCC 6803.","authors":"Namrata Bhagat, Guddu Kumar Gupta, Amritpreet Kaur Minhas, Deepak Chhabra, Pratyoosh Shukla","doi":"10.1186/s12896-025-00955-9","DOIUrl":"10.1186/s12896-025-00955-9","url":null,"abstract":"<p><strong>Background: </strong>Natural colorants produced by the cyanobacterium include carotenoids, chlorophyll a and phycocyanin. The current study used the Synechocystis sp. PCC 6803 to examine how abiotic stress conditions, such as low temperature as well as high light intensity, affect the pigment accumulations in comparison to the control conditions. Additionally, using the response surface methodology (RSM) and artificial neural network - multi-objective genetic algorithm (ANN-MOGA), the impact of several nitrogen sources such as urea, ammonium chloride, and sodium nitrate as nutritional stress on the pigment accumulations in the Synechocystis sp. PCC 6803 was examined.</p><p><strong>Results: </strong>The results showed that the pigment accumulation was more pronounced when urea and ammonium chloride was used in combination with nitrate, respectively, as nitrogen source. With the help of our prediction model that used ANN-MOGA, we were able to enhance the synthesis of chlorophyll a, carotenoids, and phycocyanin by 21.93 µg/mL, 9.78 µg/mL, and 0.05 µg/mL, respectively compared to control with 6.37, 3.88 and 0.008 µg/mL. The significant scavenging activity of pigment was showed with 7.66 ± 0.001 values of IC50. Additionally, a very good correlation of coefficient (R²) value 0.99, 0.99 and 0.92 was obtained for APX, CAT and GPX enzyme activity, respectively.</p><p><strong>Conclusions: </strong>The findings lays the groundwork for future attempts to turn cyanobacteria into a commercially viable source of natural pigments by demonstrating the benefits of using the RSM and machine learning techniques like ANN-MOGA to optimise the production of cyanobacterial pigments. The significant scavenging and antioxidant activities like CAT, GPX and APX were also shown by the pigments of the Synechocystis sp. PCC 6803. Furthermore, these machine learning tools can be used as a model to improve and optimize the yields for other metabolites production.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"23"},"PeriodicalIF":3.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of plasma kallikrein specificity of antitrypsin variants identified by phage display and partial reversion.","authors":"Sangavi Sivananthan, Tyler Seto, Negin C Tehrani, Varsha Bhakta, William P Sheffield","doi":"10.1186/s12896-025-00956-8","DOIUrl":"10.1186/s12896-025-00956-8","url":null,"abstract":"<p><strong>Background: </strong>The naturally occurring variant Alpha-1 Antitrypsin M358R (AAT M358R), modified at the P1 position of the reactive center loop (RCL), shifts its inhibitory protease target from neutrophil elastase to multiple coagulation and contact proteases, including activated plasma kallikrein (Pka; KLKB1). Our aim was to increase the specificity of AAT M358R for Pka as a potential novel therapeutic agent to treat pathological swelling arising from elevated Pka levels in patients with Hereditary Angioedema.</p><p><strong>Results: </strong>Two AAT M358R T7Select phage display libraries randomized at RCL positions P7-P3 and P2-P3' were iteratively probed with Pka. The most abundant Pka-inhibitory motifs from phage display were P7-P3, QLIPS; and P2-P3', VRRAY (mutated residues in bold). AAT variants expressing these motifs, alone or in combination, as well as six less-mutated P7-P3 revertant proteins were expressed, purified, and characterized kinetically. Variants AAT M358R (QLIPS) (designated 7-QLIPS-3) and 7-FLEPS-3 exhibited significantly enhanced selectivity for Pka (over factor XIa) by factors of 6.9 and 9.2, respectively, without increasing the stoichiometry of inhibition (SI) or decreasing the inhibition rate relative to AAT M358R. No other variants matched this profile.</p><p><strong>Conclusions: </strong>Pro substitution at P4 was found to be important for enhanced inhibition of Pka by AAT M358R. Two novel variants with this substitution are more rapid and selective inhibitors of Pka than AAT M358R and may provide better control of Pka in vivo than existing HAE therapeutics.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"22"},"PeriodicalIF":3.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}