BMC Biotechnology最新文献

{"title":"FOXK1 regulates apoptosis, migration and angiogenesis in high glucose-induced vascular endothelial cells through p-AKT/AKT signaling pathway.","authors":"He Long, Qinghua Hu, Lan Yang, Xin Li","doi":"10.1186/s12896-025-01048-3","DOIUrl":"10.1186/s12896-025-01048-3","url":null,"abstract":"<p><strong>Background: </strong>Forkhead box K1 (FOXK1) is a newly discovered gene encoding a transcription factor in the FOX family. It plays important roles in the cell cycle, cell growth, proliferation, differentiation, apoptosis, metabolism, DNA damage, drug resistance, angiogenesis, and carcinogenesis by binding to DNA to function as a transcription factor. Many studies have confirmed that FOXK1 plays a role in promoting cancer in a variety of tumors. However, the pathogenic role of FOXK1 in diabetic retinopathy (DR) is still unclear. The present study was conducted to investigate the mechanism of FOXK1 in the vascular endothelial dysfunction of DR.</p><p><strong>Methods: </strong>Human umbilical vein endothelial cells (HUVECs) were exposed to high glucose (HG) concentrations (25 mmol/L) to establish a vascular endothelial dysfunction model of DR. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the differential expression of FOXK1 in HUVECs treated with HG and low glucose (LG). CCK-8, Transwell, flow cytometry and tube formation assays were used to investigate the effect of FOXK1 on HUVECs function. To explore the downstream signaling pathway of FOXK1, Western blotting was subsequently used to detect the protein expression of p-AKT and AKT after FOXK1 was knocked down in HUVECs. The AKT inhibitor MK-2206 was used to treat HUVECs, and cell migration, apoptosis and angiogenesis were observed.</p><p><strong>Results: </strong>The results showed that FOXK1 expression was significantly increased in HUVECs cultured with high glucose. In this endothelial dysfunction model, knockdown of FOXK1 increased HUVECs activity and migration, inhibited HUVECs apoptosis, angiogenesis and VEGF expression, and activated the p-AKT/AKT signaling pathway. All of these were reversed by the AKT inhibitor MK-2206.</p><p><strong>Conclusions: </strong>FOXK1 may mediate vascular endothelial dysfunction in DR by inhibiting p-AKT/AKT pathway.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"110"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver iodide nanoparticles from Kluyveromyces marxianus M59 beta-glucan: characterization and anticancer activity.","authors":"Berat Cinar-Acar","doi":"10.1186/s12896-025-01046-5","DOIUrl":"10.1186/s12896-025-01046-5","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"111"},"PeriodicalIF":3.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapidly decellularized adipose tissue induces soft tissue vascularization in potential anatomical spaces.","authors":"Piejko Marcin, Hinz Alicja, Mak Paweł, Ligas Elwira, Misztal Krzysztof, Janowski Mirosław, Wałęga Piotr, Drukała Justyna","doi":"10.1186/s12896-025-01042-9","DOIUrl":"10.1186/s12896-025-01042-9","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"109"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Acetylcysteine as an anti-oxidant and anti-inflammatory agent in decreasing histopathological damages and oxidative stress after mercury exposure in lung tissue of rats.","authors":"Kebria Diako, Shadi Rahimi, Seyed Ali Mirbagheri Saghaleksari, Seyed Milad Mousavi Eshkelani, Niloofar Keshavarz Taramsari, Asghar Beigi Harchegani, Zhaleh Mohsenifar, Mahdi Shahriarinour, Najmeh Ranji","doi":"10.1186/s12896-025-01041-w","DOIUrl":"10.1186/s12896-025-01041-w","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"108"},"PeriodicalIF":3.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12481799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient removal of Congo Red Dye using a MgAl-LDH/cuttlebone composite adsorbent.","authors":"Ramzi Chalghaf, Saber Boubakri, Zakarya Ahmed, Bilel Hadrich, Abdesslem Ben Haj Amara, Hafsia Ben Rhaiem","doi":"10.1186/s12896-025-01043-8","DOIUrl":"10.1186/s12896-025-01043-8","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"104"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics-based analysis of the correlation between lipid metabolites and thyroid carcinoma.","authors":"Junyu Liu, Jiajun Huo, Zhi Zhang, Xiongsheng Xiao, Siyi Li, Yongkang Wu, Weijie Chen, Zhuoting Chen, Changwei Zheng, Muge Liu, Baibei Li, Guiping Zhou, Zhibin Huang, Jinquan Li, Bin Liu, Haiqing Luo, Wenyi Qin","doi":"10.1186/s12896-025-01045-6","DOIUrl":"10.1186/s12896-025-01045-6","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"107"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico screening and in vitro biological evaluation reveal Queuine as a promising MAP4K4 inhibitor for treating pancreatic cancer.","authors":"Nigar Kantarci-Carsibasi, Münteha Girgin, Nursah D Fidan, Tugba Bal, Shirin Tarbiat","doi":"10.1186/s12896-025-01033-w","DOIUrl":"10.1186/s12896-025-01033-w","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"103"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the pharmacological potential of Ganoderma lucidum against haemorrhagic and anticoagulant activities of Echis ocellatus venom.","authors":"O O Oyedara, B S Ajisebiola, O E Abioye, O A Fadare, O A Olatunji, F M Adeyemi, M N Enahoro, S F Popoola, O O Oluyide, Z A Adeyemi","doi":"10.1186/s12896-025-01044-7","DOIUrl":"10.1186/s12896-025-01044-7","url":null,"abstract":"<p><strong>Background: </strong>Echis ocellatus is a highly venomous snake that can cause serious medical complications due to the presence of toxic proteins in its venom. These proteins, such as echicetin and phospholipase A₂ (PLA2), often cause severe pathophysiology in snakebite victims. Ganoderma lucidum is recognised for its pharmacological benefits against various diseases. However, the potential of this fungus as an antivenom has not yet been reported.</p><p><strong>Objective: </strong>This study investigated the inhibitory effects of G. lucidum on haemorrhagic and anticoagulant activities induced by E. ocellatus venom, and identified its possible bioactive inhibitor compounds using in vitro, in vivo, and in silico methods.</p><p><strong>Methods: </strong>Ganoderma lucidum was extracted using methanol in a standard procedure, and varying doses (20 and 40 mg) of the extract were tested against the biological activities E. ocellatus venom. Thereafter, the extract of the G. lucidum was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis to identify its bioactive compounds. The identified compounds were docked against the catalytic active sites of echicetin and PLA2 proteins to determine the best inhibitor compound. The Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) properties of compounds were determined using the ADMETlab 2.0 web tool.</p><p><strong>Results: </strong>The extract caused 62.96 ± 1.03% and 59.25 ± 1.59% venom-induced haemorrhage inhibition at doses of 20 and 40 mg, respectively, while plasma clotting times were shortened to 132 and 163 s at 20 and 40 mg, respectively. The GC-MS identified 29 bioactive compounds from G. lucidum extract, out of which hesperidin had the highest docking scores of - 9.3 kcal/mol and - 9.9 kcal/mol against the catalytic sites of echicetin and PLA2 enzymes, respectively.</p><p><strong>Conclusion: </strong>The results indicate that G. lucidum has antivenom potential against E. ocellatus venom-induced toxic activities, and identified hesperidin as a promising compound for antivenom exploration against viper envenoming.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"105"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel single-vector featuring bidirectional promoters for production of bispecific antibody against rabies virus.","authors":"Zhigao Zhang, Jia Li, Guanfeng Lin, Shouchun Cao, Yingsong Wu","doi":"10.1186/s12896-025-01036-7","DOIUrl":"10.1186/s12896-025-01036-7","url":null,"abstract":"<p><strong>Background: </strong>The World Health Organization (WHO) recommends combination therapy with two non-overlapping monoclonal antibodies (mAbs) to enhance protection in Rabies post-exposure prophylaxis (PEP). While bispecific antibodies (bsAbs) provide superior protection over monoclonal antibodies (mAbs) by simultaneously targeting two distinct epitopes, their development is hindered by complex and inefficient vector design. In this study, we aimed to develop a novel single-vector expression system to efficiently produce potent anti-rabies virus (RABV) neutralizing bsAbs.</p><p><strong>Results: </strong>We engineered a minimalistic peaSKYA single-vector featuring bidirectional dual-CMV promoters to enable synchronous co-expression of antibody heavy (HC) and light chains (LC) within a single construct. By eliminating redundant backbone elements, this optimized vector increased the expression-cassette ratio by 17% and improves transfection efficiency by 32% compared to dual-vector systems. The platform demonstrated 2-fold greater yields of bispecific antibodies in HEK293F transient expression, with the tetravalent DVD-Ig format antibody HHDVD showing significantly enhanced neutralization potency versus parental monoclonal antibodies in both pseudovirus-based neutralization assays (PBNA, 2193.19 IU/mg) and rapid fluorescent focus inhibition tests (RFFIT, 1679.52 IU/mg).</p><p><strong>Conclusions: </strong>We developed peaSKYA single-vector expression system for robust production of bispecific antibodies, with candidate HHDVD exhibiting potential in Rabies PEP. This streamlined vector design approach exhibited remarkable potential in bispecific neutralizing antibody development, offering a transferable platform for other engineered bispecific antibodies.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"106"},"PeriodicalIF":3.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prophylactic role of artemisinin in modulating FGFR3, HRAS, and TP53 to prevent early-stage urothelial carcinoma in BBN-induced mouse models.","authors":"Silvia Botrous, Ayaat Elmaghraby, Samar El Achy, Yehia Mustafa, Salah Abdel-Rahman","doi":"10.1186/s12896-025-01039-4","DOIUrl":"10.1186/s12896-025-01039-4","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"102"},"PeriodicalIF":3.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12442291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}