BMC Biotechnology最新文献

{"title":"Biosystematics and molecular characterization of three biotypes of Convolvulus arvensis L. (convolvolaceae) in Marsa Matrouh, Egypt.","authors":"Faiza A Shehata, Muhammad A Elsobky, Marwa M Hegazy, Ehab M B Mahdy","doi":"10.1186/s12896-026-01202-5","DOIUrl":"10.1186/s12896-026-01202-5","url":null,"abstract":"<p><p>The notable characteristic of phenoplasticity in Convolvulus species is flower color polymorphism, with blooms ranging from white to pink. This variation is due to various factors, including genetic drift and natural selection. The primary objective is to conduct a study on the morphoplasticity of eight accessions collected from diverse locations and categorized into three distinct biotypes based on floral pigmentation: Biotype 1 (White; accessions 1, 4, and 7), Biotype 2 (Pale Pink; accessions 2, 5, and 8), and Biotype 3 (Pink; accessions 3 and 6) of Convolvulus arvensis L. Based on the aforementioned characteristics-flower colour, stem outline, leaf shape, pollen features, shape, colpus and pori shapes, epidermal cell shape, and the number of vascular bundles in both stem and petiole-the study employed morphological and anatomical studies, as well as CDDP markers, to evaluate differences among accessions. Leaf tissues are parenchyma above the main vascular bundle, with an angle between the midrib and the wing region. Three distinct biotypes (B1, B2, and B3) have been defined. CDDP marker analysis revealed high polymorphism (74.36%) and Rp (9.50). Samples 7 and 8 (location 3) showed the most informative polymorphism of flower color (white/pale pink/pink). Genetic distances clustered samples primarily by location rather than by color, underscoring the dominant role of location in structuring populations. The interaction between genotype and ecological conditions led to the genetic variation in flower color in C. arvensis.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13615628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149057917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Formulation and evaluation of hydrogel based on Brazilian propolis ethanol extract for promoting diabetic wound healing.","authors":"Huanglin Huo, Zhenhua Zhou, Rongxiang Guo, Jinyi Zhang, Menghan Li, Huicong Zeng, Dongyu Zhao, Roman P Terekhov, Peng Jiang, Qian Zhou, Bo Li","doi":"10.1186/s12896-026-01203-4","DOIUrl":"10.1186/s12896-026-01203-4","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13403621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico prioritization of potential matrix metalloproteinase-13 inhibitors for cartilage degeneration in knee injury-associated osteoarthritis.","authors":"Aijuan Zhang, Hongmei Chen, Na Song, Juanjuan Zhuang, Baochai Chen, Xiuying Lin, Shuang Jin, Chen Chen","doi":"10.1186/s12896-026-01200-7","DOIUrl":"10.1186/s12896-026-01200-7","url":null,"abstract":"<p><p>Knee trauma is an important risk factor for post-traumatic osteoarthritis (PTOA), a progressive joint disease characterized by articular cartilage degeneration and limited disease-modifying treatment options. Matrix metalloproteinase-13 (MMP-13/collagenase-3) contributes to cartilage extracellular matrix degradation through cleavage of type II collagen, making it a relevant molecular target for cartilage-protective drug discovery. This study aimed to identify and prioritize potential small-molecule MMP-13 inhibitors using an integrated in silico workflow. The workflow included MMP-13 sequence retrieval, structural characterization, pharmacophore-based screening, molecular docking, ADMET/toxicity prediction, protein-ligand interaction analysis, protein-protein interaction and co-expression network analysis, and 100 ns molecular dynamics simulation. Among the screened compounds, P4C-BM was prioritized as an early-stage computational lead candidate based on its combined docking score, predicted ADMET profile, interaction pattern, and molecular dynamics stability. However, the findings are preliminary and computational only. The present study does not experimentally demonstrate direct MMP-13 inhibition, selectivity against other MMP family members, cartilage repair, clinical efficacy, or synergy with platelet-rich plasma, physiotherapy, or other treatment modalities. Among the screened compounds, P4C-BM was prioritized as an early-stage computational lead candidate based on its combined docking score, predicted ADMET profile, interaction pattern, and molecular dynamics stability. However, the findings are preliminary and computational only. The present study does not experimentally demonstrate direct MMP-13 inhibition, selectivity against other MMP family members, cartilage protection, cartilage repair, disease modification, or clinical efficacy. Therefore, P4C-BM should be regarded only as a candidate for further experimental validation in enzymatic assays, chondrocyte models, cartilage explant studies, and in vivo osteoarthritis or post-traumatic osteoarthritis models. No practice-related or treatment recommendations can be made from the present data.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First breed-pool whole genome sequencing of egyptian sheep: a comprehensive genomic atlas revealing diversity and candidate genes for production and adaptation.","authors":"Nada N A M Hassanine, Ali H Amin, Elsayed E Hafez, Mohamed Morsi M Ahmed, Ahmed H Ismail, Asmaa Zayed Mohamed, Heba Abd El Halim, Ghada M El-Sayed, Ibrahim A A Adss, Khaled H Radwan, Mabrouk A Abo-Zaid, Amr M A Rashad, Hosameldeen Mohamed Husien, Ahmed A Saleh, Mengzhi Wang","doi":"10.1186/s12896-026-01199-x","DOIUrl":"10.1186/s12896-026-01199-x","url":null,"abstract":"<p><strong>Background: </strong>This research provides the first extensive breed‑pool whole‑genome sequencing (WGS) analysis across five Egyptian sheep populations: Barki (BAR), Rahmani (RAH), their crossbred offspring (CRS), Ossimi (OSI) and Awassi (AWI).</p><p><strong>Objectives: </strong>To establish a genomic atlas of the genetic architecture of production and adaptation in Egyptian sheep, providing a baseline for future candidate gene discovery and conservation strategies.</p><p><strong>Methods: </strong>Through Illumina sequencing of 120 samples, we compiled a dataset exceeding 470 Gb, with mean coverage depths spanning 24.2x to 41.3x. Variant profiling, functional annotation, KEGG pathway analysis, and independent structural variant analysis were conducted. Phenotypic data were collected and validated through qRT-PCR gene expression analysis.</p><p><strong>Results: </strong>Variant profiling revealed between 11.9 and 17.4 million SNPs per breed after stringent filtering. Heterozygosity patterns (population‑level estimates) differed substantially between groups, recorded at 60.41% in the CRS crossbred versus 74.92-85.01% in the purebred lines. Functional annotation identified conserved enrichment related to xenobiotic detoxification and lipid metabolism. KEGG pathway analysis prioritized the PPAR signalling pathway (map03320) and fatty acid metabolism (map01212) as highly significant (p < 0.0001). Independent structural variant analysis identified distinct genomic hotspots on chromosomes 2, 6 and 18, overlapping candidate genes; FABP4, KAP cluster and MSTN implicated in the regulation of fat deposition and muscle development. Phenotypic data confirmed a high degree of breed divergence (p < 0.001). RAH and CRS individuals reached higher body condition scores (BCS 4.31‑ 4.53) and increased fat deposition, whereas BAR was significantly leaner (BCS 2.53). The highest trimmed meat yields were observed in CRS (23.95 kg) and RAH (18.40 kg) (p < 0.001), with RAH also displaying the highest intramuscular fat content at 4.20%. qRT‑PCR validation showed elevated expression of lipogenic genes (ACACA, FASN and FABP4) in fat‑tailed breeds and differential expression of myogenic regulators (MSTN and IGF‑1) correlating with muscularity variations.</p><p><strong>Conclusions: </strong>The current findings establish a genomic atlas for the genetic architecture of production and adaptation in Egyptian sheep, providing a baseline for future genetic and conservation strategies. Formal selection signature analyses, such as XP‑EHH and iHS are recommended for subsequent studies.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148454379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin-loaded PCL scaffold suppresses bone metastatic tumors and augments in situ bone formation.","authors":"Lei Yang, Dan Zhang, Xiaoyan Xie, Rui Qiu, Xiaohui Yue, Min Wu, Chengjie Ji, Jingjing Zhong","doi":"10.1186/s12896-026-01198-y","DOIUrl":"10.1186/s12896-026-01198-y","url":null,"abstract":"<p><p>This study developed a dual-functional 3D-printed poly(ε-caprolactone) (PCL) scaffold loaded with quercetin (Q-PCL) for synergistic inhibition of bone metastatic tumors and promotion of bone regeneration. The scaffold was fabricated via melt extrusion, exhibiting a uniform porous structure conducive to sustained quercetin release. In vitro, Q-PCL significantly suppressed renal carcinoma (RENCA) cell proliferation and induced apoptosis, while enhancing the osteogenic differentiation and mineralization of bone marrow mesenchymal stem cells (BMSCs). In a murine subcutaneous tumor model, Q-PCL implantation effectively inhibited tumor growth via apoptosis induction without systemic toxicity. In a rat femoral defect model, the scaffold markedly accelerated bone repair, showing increased bone volume, improved trabecular morphology, and mature bone formation. The Q-PCL scaffold demonstrates great potential as a localized co-therapy strategy for treating osteolytic bone metastases and facilitating bone regeneration.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148434901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and in vitro evaluation of teicoplanin PEGylated (TEC-PEGylated) niosomes for antimicrobial and anti-biofilm activity against vancomycin-intermediate Staphylococcus aureus (VISA).","authors":"Jaber Hemmati, Zahra Chegini, Iraj Sedighi, Mehdi Azizi, Raha Zare Shahraki, Mohsen Chiani, Mohammad Reza Arabestani","doi":"10.1186/s12896-026-01201-6","DOIUrl":"10.1186/s12896-026-01201-6","url":null,"abstract":"<p><p>Vancomycin-intermediate Staphylococcus aureus (VISA) is a prominent pathogen in burn wound infections, mainly known for biofilm formation. Recently, infections associated with antibiotic-resistant VISA isolates have increasingly posed life-threatening risks. Accordingly, there is an urgent necessity to investigate and develop efficient strategies against the rapid spread of VISA strains in the healthcare system. In this research, a niosomal drug delivery system was developed using the thin-film hydration method and then surface‑modified with polyethylene glycol (PEG). Teicoplanin (TEC) was further incorporated into PEGylated niosome (TEC-PEGylated niosome), and key physicochemical properties, including encapsulation efficiency (EE %), drug release profile, particle size, surface zeta charge, morphology, polydispersity index (PDI), and 30-day stability were measured. The antimicrobial potential of TEC-PEGylated niosome against five VISA strains isolated from burn wounds was investigated by determining the minimum inhibitory/bactericidal concentrations (MIC, MBC) and the time-kill assay. Furthermore, the anti-biofilm properties of the synthesized niosomal formulation were evaluated with a microtiter-plate (MTP) method and compared to those of free drug. FE-SEM results revealed that the niosomal formulation had spherical morphology with an approximate size of 250 nm. Hydrodynamic size, surface zeta charge, and EE% of the formulated niosomes were found be 278.8 ± 5.0 nm, + 9.5 ± 3.4 mV, and 65.9% ± 1.6, respectively. Also, TEC-PEGylated niosome demonstrated a significantly improved antibacterial ability compared to free drug. Additionally, TEC-PEGylated niosome effectively inhibited the biofilm formation capacity in all VISA strains. It was concluded that synthesized niosomal system could be an effective drug delivery system owing to several advantageous characteristics, including sustained-release profiles, acceptable stability, and other desirable features. These properties enable PEGylated niosomes to effectively deliver a diverse array of antimicrobial agents, including TEC. This capability could present a promising novel strategy for addressing burn infections, particularly those caused by VISA strains.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process optimization for protoplast preparation from Grifola frondosa and development of a high-yield mutant with enhanced mycelial growth and protein production.","authors":"Qiaoyu Wang, Ziteng Zhao, Qiuyu Lu, Ke'er Xiao, Lingyu Gao, Mukaddas Sai, Lili Guan, Linna Du","doi":"10.1186/s12896-026-01197-z","DOIUrl":"10.1186/s12896-026-01197-z","url":null,"abstract":"<p><p>Grifola frondosa is a highly valued medicinal mushroom with diverse bioactive properties; however, its low mycelial biomass remains a major bottleneck affecting the efficiency of liquid fermentation. To address this issue, protoplast-mediated mutagenesis was employed in this study to breed a high-performance G. frondosa mutant with superior fermentation traits. Following optimization using intelligent strategies, the highest yield of protoplasts was achieved (6.573 × 10⁶ protoplasts/g fresh weight) under suitable isolation parameters (fungal age of 7.9 days, lywallzyme concentration 2.1%, enzymatic hydrolysis time 5.2 hours, enzymatic temperature of 30 °C, and mannitol concentration of 0.6 mol/L). Subsequently, DES-induced protoplast mutagenesis yielded a stable G. frondosa mutant (D24) showing 62.78% greater mycelial biomass and 111.11% higher protein yield relative to the parental strain. Furthermore, fermentation kinetics profiling and phenotypic observation suggested that the mutant D24 exhibited a shortened lag phase, enhanced hyphal dispersion, and denser mitochondrial distribution compared with the original strain. Additionally, genome resequencing identified 57,216 single nucleotide polymorphisms (SNPs) in D24 relative to the original strain, including 4,068 non-synonymous SNPs. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment indicated that the mutated genes were potentially involved in amino acid synthesis and metabolism, lipid metabolism, and others. These candidate mutations may be associated with the increased mycelial biomass and protein content. Collectively, this study has developed a novel high-yielding mutant of G. frondosa, which may serve as a promising material for future pharmaceutical and biotechnological applications.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13628877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.).","authors":"Xiaoyuan Xi, Jia Song, Mengqing Feng, Xiaodong Qian, Heng Sun, Liqin Li","doi":"10.1186/s12896-026-01187-1","DOIUrl":"10.1186/s12896-026-01187-1","url":null,"abstract":"<p><strong>Background: </strong>Protoplasts have been widely utilized in tissue culture, single-cell RNA sequencing, and transient gene expression analyses. However, the primary challenge in their preparation lies in the efficient removal of rigid cell walls to obtain high-quality plant cells. The aim of this study is to establish an effective method for protoplast isolation from saffron (Crocus sativus L.), thereby accelerating advances in molecular biology research in this species.</p><p><strong>Result: </strong>Tissue-specific protoplasts were successfully isolated from apical buds, petals, leaves, and roots of saffron, with yields of approximately 8.81 × 10⁶, 1.36 × 10⁷, 7.84 × 10⁵, and 5.69 × 10⁵/g FW, and viabilities of ~ 95%, ~ 94%, ~ 96%, and ~ 61%, respectively. The optimal enzymatic digestion conditions varied by tissue type: apical buds (0.6 M D-mannitol, 1.5% cellulase, 0.5% macerozyme, 28 °C, 3 h); roots (0.7 M D-mannitol, 2.0% cellulase, 0.5% macerozyme, 0.5% pectinase, 28 °C, 4 h); petals (0.7 M D-mannitol, 1.5% cellulase, 0.5% macerozyme, 28 °C, 3.5 h); and mesophyll (0.7 M D-mannitol, 1.5% cellulase, 0.5% macerozyme, 28 °C, 4 h). The quality of the protoplasts was affected by developmental stages and sampling locations. Furthermore, two high-quality single-cell libraries were constructed using protoplasts from non-flowering and flowering apical buds, respectively. We successfully visualized the subcellular localization of GFP in the mesophyll protoplasts.</p><p><strong>Conclusion: </strong>This study presents the first detailed report on protoplast isolation, evaluation, and application in saffron. By overcoming the technical challenges of tissue-specific protoplast isolation from diverse organs, we established a highly efficient method that enables both the development of novel germplasm and mechanistic insights into saffron organogenesis, growth, and development.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13352814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual antimicrobial and anticancer functions of artemisinin-loaded PEG₄₀₀-oa nanoparticles: synergistic activity with ciprofloxacin and mechanistic insights from molecular docking.","authors":"Asma Seyedan, Mahdi Shahriarinour, Somayeh Ataei Jaliseh, Najmeh Ranji, Hossein Zahmatkesh","doi":"10.1186/s12896-026-01195-1","DOIUrl":"https://doi.org/10.1186/s12896-026-01195-1","url":null,"abstract":"<p><p>Artemisinin, a bioactive compound from Artemisia annua with potent antioxidant, antimicrobial and anticancer properties, faces limited clinical utility due to poor solubility and low bioavailability. Given the rising prevalence of multidrug-resistant (MDR) Pseudomonas aeruginosa alternative therapeutic strategies are essential. This study synthesized and characterized artemisinin-loaded micelle/liposome nanoparticles (Art/PEG₄₀₀-OA NPs) and evaluated their antibacterial, antibiofilm, and anticancer efficacy. Artemisinin was successfully encapsulated within PEG₄₀₀-OA NPs and characterized via FT-IR, FE-SEM, TEM, TGA, and zeta potential measurements. The nanoparticles exhibited uniform morphology, particle sizes of 40.8 nm (TEM) and 149.8 nm (FE-SEM), high encapsulation efficiency (92.14%), and strong thermal and colloidal stability. Synergistic and partial synergistic interactions between Art/PEG₄₀₀-OA NPs and ciprofloxacin were observed in MDR bacterial samples, significantly inhibiting biofilm formation. Quantitative RT-PCR analysis revealed the downregulation of efflux pump genes (mexA, mexB, mexX, mexY, oprM) and biofilm-associated genes (algD, pelA) following combination therapy. In AGS gastric cancer cells, Art/PEG₄₀₀-OA NPs induced dose- and time-dependent cytotoxicity (IC₅₀: 67.75, 62.25 and 54.98 µg/mL at 24, 48 and 72 h), increased apoptosis (31.76% ± 4.2%), and reduced wound-healing migration (56.65% ± 4.26%). Expression analysis demonstrated upregulation of tumor-suppressive miR-34a, and downregulation of its targets (CTNNB1, TGFB1, MMP2 and MMP9). Conversely, miR-181a was downregulated, leading to the upregulation of apoptotic target genes (TP53, CASP8, CASP9). Molecular docking supported strong artemisinin binding to key bacterial and oncogenic proteins. Overall, Art/PEG₄₀₀-OA nanoparticles represent a potent dual-action nanotherapeutic platform against antibiotic-resistant infections and gastric cancer.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Expression of Concern: Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus.","authors":"Ahmed N Alabssawy, Mohammed Abu-Elghait, Ahmad M Azab, Hassan M M Khalaf-Allah, Abdelrahman S Ashry, Ahmed O M Ali, Abu-Bakr A A Sabra, Salem S Salem","doi":"10.1186/s12896-026-01192-4","DOIUrl":"10.1186/s12896-026-01192-4","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13317312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148350562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}