BMC Biotechnology最新文献

{"title":"Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques.","authors":"Mustafa Sevindik, Celal Bal, Tetiana Krupodorova, Ayşenur Gürgen, Emre Cem Eraslan","doi":"10.1186/s12896-025-00960-y","DOIUrl":"https://doi.org/10.1186/s12896-025-00960-y","url":null,"abstract":"<p><p>In this study, the biological activities of Otidea onotica were investigated using two optimization methods, Response Surface Methodology (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA). The extracts were tested for phenolic content, antioxidant potential, acetylcholinesterase and butyrylcholinesterase inhibitory activities and antiproliferative effects against A549 lung cancer cell line. The results show that the extracts obtained by ANN-GA optimization exhibited higher antioxidant activity compared to RSM extracts and had higher total antioxidant status (TAS), DPPH and FRAP values. Phenolic content analysis revealed eight phenolic compounds and the compounds with the highest concentrations were caffeic acid (in RSM extract) and gallic acid (in ANN-GA extract), respectively. Both extracts showed strong cytotoxic effects against A549 cells depending on the concentration, with ANN-GA extract showing higher antiproliferative activity. Our study provides important findings on the biological activities and therapeutic potential of O. onotica and particularly reveals that the ANN-GA optimization method plays an important role in increasing biological activity. The findings show that O. onotica extracts can be used in the treatment of cancer and neurodegenerative diseases in the future and that optimization techniques offer an effective strategy for enriching phenolic contents.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"25"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro.","authors":"Oksana Mykchaylova, Aleksander Besarab, Anatoliy Negriyko, Margarita Lomberg, Natalia Poyedinok","doi":"10.1186/s12896-025-00957-7","DOIUrl":"10.1186/s12896-025-00957-7","url":null,"abstract":"<p><strong>Background: </strong>The problem of searching for environmentally friendly regulators of the biosynthetic activity of edible and medicinal mushrooms is crucial for creating highly effective biotechnologies. One such regulator is light. This study aimed to compare and evaluate the fatty acid profile and fat quality indices of lipids from the mycelial mass of Pleurotus eryngii under various light-emitting diode (LED) and laser light irradiation regimes.</p><p><strong>Methods: </strong>To determine the effect of artificial light on the biosynthetic activity of P. eryngii, an artificial lighting system based on LED matrices with wavelengths of 470 nm (blue), 530 nm (green), and 650 nm (red), as well as an argon gas laser as a coherent visible light source at 488 nm, was used. For all experimental variants, the energy density on the surface of the samples was set to the same, providing an energy dose of 240 mJ/cm². Irradiation was carried continuously.</p><p><strong>Results: </strong>Twenty-seven fatty acids were identified in the studied P. eryngii mycelial mass samples, including nine saturated fatty acids (SFAs), eight monounsaturated fatty acids (MUFAs) and ten polyunsaturated fatty acids (PUFAs). The control sample (without irradiation) contained the lowest number of fatty acids (fourteen). With irradiation in all modes, a decrease in the amount of SFA and the formation of new MUFA and PUFA with a chain length of C20-C24, which were absent in the control, were observed. Blue light stimulated the synthesis of significant amounts of α-linolenic acid (C18:3ω-3). The ratios of ΣPUFA/ΣSFA, ΣPUFA/ΣMUFA and ΣPUFAω-6/ω-3 in the mass of mycelium irradiated with blue light were within the optimal values for the human diet.</p><p><strong>Conclusions: </strong>The selected mycelial photoactivation modes using low-intensity laser and LED light of different spectral composition and coherence may have potential in the biotechnology of submerged cultivation of P. eryngii to obtain mycelial mass with an improved fatty acid profile, which can be considered as a useful source of lipids.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"24"},"PeriodicalIF":3.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Neural Network - Multi-Objective Genetic Algorithm based optimization for the enhanced pigment accumulation in Synechocystis sp. PCC 6803.","authors":"Namrata Bhagat, Guddu Kumar Gupta, Amritpreet Kaur Minhas, Deepak Chhabra, Pratyoosh Shukla","doi":"10.1186/s12896-025-00955-9","DOIUrl":"10.1186/s12896-025-00955-9","url":null,"abstract":"<p><strong>Background: </strong>Natural colorants produced by the cyanobacterium include carotenoids, chlorophyll a and phycocyanin. The current study used the Synechocystis sp. PCC 6803 to examine how abiotic stress conditions, such as low temperature as well as high light intensity, affect the pigment accumulations in comparison to the control conditions. Additionally, using the response surface methodology (RSM) and artificial neural network - multi-objective genetic algorithm (ANN-MOGA), the impact of several nitrogen sources such as urea, ammonium chloride, and sodium nitrate as nutritional stress on the pigment accumulations in the Synechocystis sp. PCC 6803 was examined.</p><p><strong>Results: </strong>The results showed that the pigment accumulation was more pronounced when urea and ammonium chloride was used in combination with nitrate, respectively, as nitrogen source. With the help of our prediction model that used ANN-MOGA, we were able to enhance the synthesis of chlorophyll a, carotenoids, and phycocyanin by 21.93 µg/mL, 9.78 µg/mL, and 0.05 µg/mL, respectively compared to control with 6.37, 3.88 and 0.008 µg/mL. The significant scavenging activity of pigment was showed with 7.66 ± 0.001 values of IC50. Additionally, a very good correlation of coefficient (R²) value 0.99, 0.99 and 0.92 was obtained for APX, CAT and GPX enzyme activity, respectively.</p><p><strong>Conclusions: </strong>The findings lays the groundwork for future attempts to turn cyanobacteria into a commercially viable source of natural pigments by demonstrating the benefits of using the RSM and machine learning techniques like ANN-MOGA to optimise the production of cyanobacterial pigments. The significant scavenging and antioxidant activities like CAT, GPX and APX were also shown by the pigments of the Synechocystis sp. PCC 6803. Furthermore, these machine learning tools can be used as a model to improve and optimize the yields for other metabolites production.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"23"},"PeriodicalIF":3.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of plasma kallikrein specificity of antitrypsin variants identified by phage display and partial reversion.","authors":"Sangavi Sivananthan, Tyler Seto, Negin C Tehrani, Varsha Bhakta, William P Sheffield","doi":"10.1186/s12896-025-00956-8","DOIUrl":"10.1186/s12896-025-00956-8","url":null,"abstract":"<p><strong>Background: </strong>The naturally occurring variant Alpha-1 Antitrypsin M358R (AAT M358R), modified at the P1 position of the reactive center loop (RCL), shifts its inhibitory protease target from neutrophil elastase to multiple coagulation and contact proteases, including activated plasma kallikrein (Pka; KLKB1). Our aim was to increase the specificity of AAT M358R for Pka as a potential novel therapeutic agent to treat pathological swelling arising from elevated Pka levels in patients with Hereditary Angioedema.</p><p><strong>Results: </strong>Two AAT M358R T7Select phage display libraries randomized at RCL positions P7-P3 and P2-P3' were iteratively probed with Pka. The most abundant Pka-inhibitory motifs from phage display were P7-P3, QLIPS; and P2-P3', VRRAY (mutated residues in bold). AAT variants expressing these motifs, alone or in combination, as well as six less-mutated P7-P3 revertant proteins were expressed, purified, and characterized kinetically. Variants AAT M358R (QLIPS) (designated 7-QLIPS-3) and 7-FLEPS-3 exhibited significantly enhanced selectivity for Pka (over factor XIa) by factors of 6.9 and 9.2, respectively, without increasing the stoichiometry of inhibition (SI) or decreasing the inhibition rate relative to AAT M358R. No other variants matched this profile.</p><p><strong>Conclusions: </strong>Pro substitution at P4 was found to be important for enhanced inhibition of Pka by AAT M358R. Two novel variants with this substitution are more rapid and selective inhibitors of Pka than AAT M358R and may provide better control of Pka in vivo than existing HAE therapeutics.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"22"},"PeriodicalIF":3.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extract optimization of Ulva lactuca L. and biological activities of optimized extracts.","authors":"Nuh Korkmaz","doi":"10.1186/s12896-025-00954-w","DOIUrl":"10.1186/s12896-025-00954-w","url":null,"abstract":"<p><strong>Background: </strong>Algae are microscopic or macroscopic organisms that can photosynthesize and are found in both freshwater and saltwater ecosystems and are considered one of the cornerstones of life. In our study, the biological activities of extracts produced under the extract conditions that provided the highest biological activity of Ulva lactuca L. were determined.</p><p><strong>Methods: </strong>Two different methods, Response Surface Method (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA) integration were used for optimization. In this context, the optimum extract conditions were determined as 54.940 ˚C temperature, 6.513 h, 42.109 ethanol/water ratio according to the RSM method and 56.286 ˚C temperature, 7.2793 h, 36.8625 ethanol/water ratio according to ANN-GA. The antioxidant activities of the optimized extracts were evaluated by Rel Assay kits, DPPH and FRAP methods. Anticholinesterase activities were determined by testing against acetylcholinesterase and butyrylcholinesterase enzymes. In addition, antiproliferative effects were examined in A549 lung cancer cell line and phenolic compound contents were analyzed by LC-MS/MS.</p><p><strong>Results: </strong>As a result of the analyzes, it was seen that the extract obtained from the conditions recommended by ANN-GA exhibited higher activities. Optimized extracts of U. lactuca were found to have potential in terms of antioxidant activities. The highest total antioxidant value was determined as 6.272 ± 0.024 mmol/L. In addition, it was determined that extracts of U. lactuca produced under optimum conditions showed strong cytotoxic effects against A549 lung cancer cell line. In addition, gallic acid, 4-hydroxybenzoic acid, caffeic acid, vanillic acid, syringic acid, quercetin and kaempferol were found in the extracts of the algae produced under optimum conditions. The highest detected compound was caffeic acid. In addition to all these properties, it was seen that U. lactuca has anticholinesterase potential in our study.</p><p><strong>Conclusion: </strong>In conclusion, optimized extracts of U. lactuca attract attention with their antioxidant and cytotoxic activities as well as anticholinesterase potential and can be considered as a potential source for cancer treatment and management of neurological diseases.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"21"},"PeriodicalIF":3.5,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing lipid accumulation in Tetradesmus obliquus under heavy metal stress for sustainable biodiesel innovation.","authors":"Eman A Alwaleed, Hamdy R M Galal, Mohamed Aboueldahab, Hani Saber","doi":"10.1186/s12896-025-00951-z","DOIUrl":"10.1186/s12896-025-00951-z","url":null,"abstract":"<p><p>This study investigates the potential of Tetradesmus obliquus for lipid accumulation under heavy metal stress and evaluates it's aviability for biodiesel production. We surveyed how different concentrations of heavy metals, including manganese (Mn), cobalt (Co), and zinc (Zn), influence the carbohydrate & protein, lipid yield, and fatty acid profiles of T. obliquus cultures. Our results demonstrated that while lipid content increased under heavy metal stress, the extent of accumulation was highly dependent on metal type and concentration. Notably, the algal culture treated with 0.04 mM Co²⁺ showed the highest lipid accumulation. Treatment with 0.3 mM Zn²⁺ resulted in the highest proportion of saturated fatty acids (SFA). The Relative Enrichment Efficiency Coefficient (REEC) analysis demonstrated that 0.04 mM and 0.07 mM Co²⁺ led to the highest lipid and carbohydrate content stimulation. Additionally, GC-MS analysis revealed increased monounsaturated fatty acids (MUFA) under several metal stress conditions. The study demonstrated that exposure to specific concentrations of heavy metals can significantly enhance lipid accumulation and alter the fatty acid profiles of T. obliquus, which are crucial for improving biodiesel quality. The implications of these findings suggest that heavy metal-induced stress could be a feasible approach to enhancing lipid accumulation for sustainable biodiesel production, and T.obliquus is a promising candidate for future biodiesel production.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"20"},"PeriodicalIF":3.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal efficacy of Citrusfusion mediated silver nanoparticles in Candida species.","authors":"A Harine, S Ranjani, S Hemalatha","doi":"10.1186/s12896-025-00952-y","DOIUrl":"10.1186/s12896-025-00952-y","url":null,"abstract":"<p><strong>Background: </strong>Candida species are commensal fungi that can become opportunistic pathogens under specific host and environmental conditions. The emergence of multidrug-resistant Candida strains poses a significant challenge. Nanotechnology represents a cutting-edge field offering precise and targeted delivery systems for combating fungal infections, leveraging the unique properties of plant-derived bioactive compounds. This investigation employed a biogenic approach utilizing polyherbal leaf extracts from Citrus limon and Citrus medica, known for their abundant Citral content.</p><p><strong>Results: </strong>Citrus sp. extracts were used to synthesize Citrusfusion silver nanoparticles (CitAgNPs) through a green synthesis method. Characterization of CitAgNPs was carried out using advanced analytical methods ensuring the quality, uniformity, size, and charge. The synthesized CitAgNPs exhibited non toxic effect when tested on Vigna radiata and Danio rerio, highlighting their potential for sustainability and safe therapeutic use. Antifungal assays demonstrated the potent efficacy of CitAgNPs in various Candida strains, with low MIC and MFC. CitAgNPs exhibited remarkable biofilm inhibition capabilities and elucidated specific mechanisms of action in Candida species, surpassing the performance of fluconazole.</p><p><strong>Conclusion: </strong>This study underscores the immense potential of nanotechnology-driven approaches harnessing Citrus leaf extract for synthesizing highly effective antifungal nanoparticles. The fusion of biogenic nanoparticles with Citrus bioactive compounds presents a sustainable strategy for addressing the escalating challenge of azole-resistant Candida infections. The research outcomes suggest that CitAgNPs have promising applications in inhibiting Candida biofilms, offering potential solutions for infections caused by diaper rashes and onychomycosis, providing safe and effective alternatives to antifungal therapies.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"18"},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic antibacterial activity of chitosan-polyethylene glycol nanocomposites films containing ZIF-8 and doxycycline.","authors":"Fahimeh Jamiri, Bahar Nayeri Fasaei, Seyed Mehdi Joghataei, Ramak Yahyaraeyat, Azin Mazloom-Jalali","doi":"10.1186/s12896-025-00953-x","DOIUrl":"10.1186/s12896-025-00953-x","url":null,"abstract":"<p><strong>Background: </strong>Antibiotic resistance is a growing global threat due to antibiotic overuse and limited treatment options. Multidrug-resistant bacteria, like Staphylococcus aureus and Escherichia coli, increase infection complexity and mortality. This study explores nanocomposite films of ZIF-8 nanoparticles and Doxycycline (Dox) to enhance antibacterial efficacy. In this study, nanocomposite films composed of chitosan (CS) and polyethylene glycol (PEG), incorporating zeolitic imidazolate framework-8 (ZIF-8) nanoparticles and DOX, were developed. These films were characterized by their morphological, mechanical, antibacterial, and drug-release properties. Antibacterial efficacy was evaluated using disk diffusion, broth microdilution, and checkerboard assay methods to determine MICs and potential synergistic effects.</p><p><strong>Results: </strong>The nanocomposite films demonstrated flexibility, semi-transparency, and a yellowish-brown hue, with films containing ZIF-8 nanoparticles being thicker (79 ± 0.2 μm) than those without (54 ± 0.5 μm). The tensile strength was enhanced with the incorporation of ZIF-8, peaking at 53.12 MPa for the CS-PEG-G-10% DOX-4% ZIF-8 film. XRD analysis confirmed the crystallinity of the ZIF-8 and DOX, with distinct peaks observed for each material. The drug release studies revealed an initial burst followed by sustained release, with higher release rates in acidic environments compared to neutral and alkaline media. The CS-PEG-G-10% DOX-4% ZIF-8 nanocomposite film demonstrated significantly higher antibacterial activity, achieving the lowest MIC values, particularly against S. aureus (22.5 mm inhibition zone) compared to E. coli (14 mm inhibition zone). Additionally, a notable synergistic effect was observed between CS-PEG-G-10% DOX and CS-PEG-G-10% DOX, with FICI values below 0.5.</p><p><strong>Conclusions: </strong>The CS-PEG-G-10% DOX-4% ZIF-8 nanocomposite exhibits enhanced antibacterial efficacy and optimal properties, positioning it as a strong candidate for developing effective treatments against multidrug-resistant pathogens.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"19"},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of cellulose-based card for direct genetic testing of spinal muscular atrophy.","authors":"Yogik Onky Silvana Wijaya, Mawaddah Ar Rochmah, Dian Kesumapramudya Nurputra, Arta Farmawati","doi":"10.1186/s12896-024-00938-2","DOIUrl":"10.1186/s12896-024-00938-2","url":null,"abstract":"<p><strong>Background: </strong>Spinal muscular atrophy (SMA) is a devastating neuromuscular condition resulting from the loss of the survival motor neuron 1 (SMN1) gene. Precise genetic testing has become essential after the authorization of several potent medications. To achieve this objective, the use of dried blood spot (DBS) has assured convenient and extensive testing from a distance. Nevertheless, developing countries such as Indonesia sometimes lack access to standard filter papers like FTA or Guthrie cards for DBS processing. Here, we aim to develop a cellulose-based card as an alternative filter paper for DBS preparation suitable for the genetic testing of SMA including but not limited to a direct polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiplex allele-specific amplification (multi-ASA).</p><p><strong>Results: </strong>An in-house paper was developed from a 180 gsm cellulose-based paper and was used for DBS preparation. The performance of dried blood spotted on the cellulose-based card (DBSc) was compared to pure genomic DNA (gDNA) isolate and dried blood spotted on FTA cards (DBSf) for genetic testing. The results of the genetic testing of our cellulose-based card were completely matched with those of gDNA and DBSf in both direct PCR-RFLP and Multi-ASA to separate SMN1 from SMN2. In addition, after three months of storing, the DBSc continued to exhibit a clear result, suggesting its high stability for DNA storage.</p><p><strong>Conclusion: </strong>Our cellulose-based card has the potential to be used for DBS carrier and for further genetic testing using PCR. Our findings can assist physicians in sending DBS samples from SMA suspicion cases to genetic testing centers, thereby preventing diagnosis delay or misdiagnosis.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"17"},"PeriodicalIF":3.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and assessment of lipase-CuO nanoparticle conjugates for enhanced antimicrobial efficacy against clinical pathogens.","authors":"Eman M Handak, Dina H Amin, Mai M Elhateir","doi":"10.1186/s12896-025-00950-0","DOIUrl":"10.1186/s12896-025-00950-0","url":null,"abstract":"<p><p>In the battle against clinical infections particularly the resistant pathogens, the creation of new antimicrobial drugs is essential. This study focuses on synthesis and characterization of Lipase-CuO nanoparticle conjugates in order to investigate their antibacterial efficiency. Lipase enzyme and CuO nanoparticles were synthesized biologically by specific selected fungal strains. Statistical optimization of lipase enzyme was done using a Plackett-Burman design giving two enhancement models for lipase production with increasing in productivity up to 143.43% (2800 U/ml). Copper oxide (CuO) nanoparticles were characterized using visual indication of greenish color formation, UV-vis spectrum analysis which revealed a strong peak at 300 nm. Also, CuO nanoparticles appeared as distinct, well-dispersed spherical particles with average size of 71.035 nm using TEM, while conjugate appears as large protein molecules linked to the nanoparticles. Also, using techniques like energy dispersive X-ray (EDAX) the resultant conjugates formation was confirmed as the elemental analysis approved its formation. The antimicrobial activity of Lipase-CuO nanoparticles conjugates was tested against a range of clinical pathogens. The results demonstrated a significant increase in antimicrobial potency compared to both CuO nanoparticles and lipase alone particularly against E. coli strain NRC B-3703 with remarkable increase of 373.6% and 75% followed by S. aureus with increase of 50 and 42.8%compared to that of individual CuO nanoparticles and lipase enzyme, respectively. These findings suggest that Lipase-CuO nanoparticle conjugates hold great promise as a novel antimicrobial strategy, offering a potential solution to combat bacterial infections, especially those caused by multidrug-resistant strains. The study highlights the importance of nanotechnology in enhancing the efficacy of traditional antimicrobial agents and opens new avenues for targeted antimicrobial therapies.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":"25 1","pages":"16"},"PeriodicalIF":3.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}