Behavioural Brain Research最新文献

{"title":"CB1 and CB2 receptors differentially modulate the cognitive impact of maternal immune activation and perinatal cannabinoid exposure","authors":"Han-Ting Chen ,&nbsp;Ken Mackie","doi":"10.1016/j.bbr.2025.115543","DOIUrl":"10.1016/j.bbr.2025.115543","url":null,"abstract":"<div><div>Maternal immune activation (MIA) commonly arises in response to an infection during pregnancy. MIA elevates cytokine levels, triggering an inflammatory cascade, which may be detrimental to the developing nervous system. Similarly, cannabis use and exposure of the fetus to cannabinoids during pregnancy (PCE) may elicit neuroinflammation and lead to detrimental behavioral outcomes. This is particularly concerning as there has been a notable rise in cannabis use during pregnancy. This study endeavors to examine the interaction between MIA and PCE and elucidate the role of CB1 and CB2 receptors in MIA and PCE outcomes. To this end, we compared the impact of MIA, PCE and MIA+PCE in wildtype, CB1, and CB2 cannabinoid receptor knockout mice of both sexes. PCE was modeled by daily 3 mg/kg THC administration from gestational day 5 (GD5) to postnatal day 10. MIA was modeled by intravenous Poly (I:C) injection at GD16.5. Subsequently, we assessed emotional and cognitive behaviors of adult offspring. Adult male offspring of dams exposed to PCE or MIA were impaired in novel object recognition and the delayed alternation working memory tasks. Interestingly, these behavioral impairments were absent when MIA and PCE were combined. Cannabinoid receptor knockout studies found that CB1 receptors mediated behavioral deficits after PCE. In contrast CB2 receptors were necessary for full expression of MIA-induced behavioral impairments. Although females showed more modest behavioral changes after MIA or PCE, CB1 receptors were required for the PCE deficit and CB2 receptors were required for the MIA deficit also in females. Notably, lack of CB2 receptors in males prevented the “protection” following combined MIA + PCE, while CB1 knockout mice remained protected. Taken together, these results suggest a complex interplay between PCE, MIA and CB1 and CB2 cannabinoid receptors.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115543"},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subjective cognitive decline in conjunction with cerebrospinal fluid anti-ATP1A3 autoantibodies and a low amyloid β 1–42/1–40 ratio: Report and literature review","authors":"Niels Hansen ,&nbsp;Kristin Rentzsch ,&nbsp;Anne Elisa Sagebiel ,&nbsp;Sina Hirschel ,&nbsp;Björn Hendrik Schott ,&nbsp;Dirk Fitzner ,&nbsp;Jens Wiltfang ,&nbsp;Claudia Bartels","doi":"10.1016/j.bbr.2025.115541","DOIUrl":"10.1016/j.bbr.2025.115541","url":null,"abstract":"<div><h3>Background</h3><div>Animal studies reveal the role of the sodium/potassium transporting ATPase α-3 subunit (ATP1A3) in maintaining the resting membrane potential and thus in synaptic information processing and potentially cognitive disorders. However, autoantibodies against AT1A3 have not previously been reported in patients with subjective cognitive decline.</div></div><div><h3>Case presentation</h3><div>We report the case of a 57-year-old female who underwent neuropsychological testing, magnetic resonance imaging (MRI) and 18 F fluorodesoxyglucose positron emission tomography (FDG-PET) imaging, and cerebrospinal fluid (CSF) analysis. Neural autoantibodies were assessed in serum and CSF. We found a normal cognitive profile together with a self-reported cognitive decline, and such consistent with subjective cognitive decline (SCD). Analysis of the cerebrospinal fluid revealed anti-ATP1A3 autoantibodies. ATP1A3 autoantibodies were also detected in serum. Analysis of amyloid pathology markers in the CSF showed a slightly reduced amyloid β1–42/ amyloid β1–40 ratio. In view of the possible paraneoplastic autoantibodies, whole-body FDG-PET was performed, which did not reveal a malignancy-specific lesion. FDG-PET of the brain also showed no hypometabolism. We diagnosed SCD based on CSF-affirmed possible Alzheimer´s pathologic change with ATP1A3 autoantibodies in CSF and serum.</div></div><div><h3>Conclusions</h3><div>To our knowledge, this is the first report of CSF and serum ATP1A3 autoantibodies associated with SCD although an incidental finding cannot be fully excluded. In addition, amyloid pathology was detected via CSF biomarkers, suggesting that ATP1A3 autoantibodies are a potentially promising biomarker in SCD with an Alzheimer´s pathologic change if confirmed in large-scale studies.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115541"},"PeriodicalIF":2.6,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RAGE/AP-1/OTR signaling pathway in rat hippocampus DG involved in CUS induced depressive-like behaviors","authors":"Xuemei Li ,&nbsp;Xin Wang ,&nbsp;Lifen Xue,&nbsp;Lan Luo,&nbsp;Lingxiao Hu,&nbsp;Wengao Jiang","doi":"10.1016/j.bbr.2025.115540","DOIUrl":"10.1016/j.bbr.2025.115540","url":null,"abstract":"<div><div>There has been a growing body of evidence indicating that the oxytocin (OT) system plays a significant role in the neurophysiology of chronic stress-related mood disorders in recent years. However, the precise alterations for the OT system in response to chronic stress and the underlying mechanism remains unclear. The present study demonstrated that chronic unpredictable stress (CUS) resulted in a reduction in the expression of RAGE and OTR, as well as an inhibition of AP-1 phosphorylation. RAGE knockdown in hippocampus DG induced depressive-like behaviors, down-regulated the OTR protein and mRNA levels, and reduced the AP-1 phosphorylation. The administration of OT via the nasal route reversed the depressive-like behaviors induced by RAGE knockdown, increased the levels of BDNF expression and AP-1 phosphorylation. On the other hand, RAGE over-expression in the hippocampus DG resisted the effects of CUS on depression-like behaviors, AP-1 phosphorylation, and OTR expression. These finding suggested that RAGE signaling pathway is involved in CUS induced depressive-like behaviors at least partially by regulating OTR expression.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115540"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Music boosts the recovery of attention after mental fatigue in healthy young male subjects: A human auditory event-related potential study","authors":"Zhiding Wang ,&nbsp;Wenhao Xu ,&nbsp;Cheng Zhang ,&nbsp;Chaoyue Zhang ,&nbsp;Yinji Liu ,&nbsp;Pinhong Chen ,&nbsp;Gencheng Han ,&nbsp;Lubin Wang","doi":"10.1016/j.bbr.2025.115539","DOIUrl":"10.1016/j.bbr.2025.115539","url":null,"abstract":"<div><div>Daily life faces continuous cognitive tasks. Several methods could lessen cognitive fatigue including music. To find out how music functions in recovering cognitive fatigue, twenty-seven participants were randomly assigned to the rest group (N = 12) and the music group (N = 15). To evaluate the effects of Mozart K488 music on attention function after a continuous cognitively demanding task. Participants completed subjective questionnaires and the contingent negative variation (CNV) task before fatigue, after fatigue, and after the rest/musical intervention. EEG and ECG data were also collected during the experiment. The results showed that 5 min of Mozart K488 music resulted in improved CNV task performance in the musical intervention group. For EEG data, recoveries of the initial CNV and terminal CNV amplitude in Cz and CPz electrodes were observed and compared with the values after Mental Fatigue, which music increased the iCNV and tCNV. Alpha-ERD was lower after listening to music than after resting. Moreover, during music playing, compared to other brain regions the EEG alpha power of participants was significantly high in the central frontal region. This study demonstrates a short-term musical intervention can effectively boost the recovery of attention after Mental Fatigue.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115539"},"PeriodicalIF":2.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of analgesic and anxiolytic effects of alcoholic extracts from Eryngium billardieri and Urtica dioica in a rat model of chronic pain","authors":"Mohammad Hosein Naeimi Ghahroodi ,&nbsp;Zahra Bahari ,&nbsp;Mahdi Mashhadi Akbar Boojar","doi":"10.1016/j.bbr.2025.115537","DOIUrl":"10.1016/j.bbr.2025.115537","url":null,"abstract":"<div><h3>Objective</h3><div>The treatment of neuropathic pain is crucial, as it not only alleviates physical discomfort but also reduces anxiety associated with pain, ultimately enhancing the quality of life for affected individuals. This study investigates the protective effects of hydro-alcoholic extracts from <em>Eryngium billardieri</em> (Er) and <em>Urtica dioica</em> (Ur) on neuropathic pain and anxiety responses in an animal model.</div></div><div><h3>Methods</h3><div>40 male Wistar rats were used to investigate neuropathy induced by the chronic constriction injury (CCI) model. Animals were divided into five experimental groups (N = 8): [sham], [CCI], [CCI+Er], [CCI+Ur], and [CCI+Imipramine]. Er and Ur were administered orally for 30 days, starting on the day of surgery. Behavioral tests, including acetone for cold allodynia, the elevated plus maze for anxiety-like behaviors, and the open field for innate anxiety, were conducted on day −1 (before CCI) and on days 2, 4, 6, 14, 21, and 30. Data were analyzed by one-way analysis of variance test, and P &lt; 0.05 was considered significant.</div></div><div><h3>Results</h3><div>Neuropathic surgery resulted in cold allodynia and anxiety-like behaviors throughout the experiment as compared to the sham. Er extract significantly decreased both cold allodynia and anxiety-like behaviors in the CCI group. However, Ur extract only significantly reduced cold allodynia (and not anxiety-like behaviors). Additionally, there was no difference between the analgesic effects of Er and Ur extracts.</div></div><div><h3>Conclusions</h3><div>These findings support the use of Er as a potential comprehensive treatment for neuropathic pain and anxiety symptoms. Future research should focus on exploring the specific mechanisms behind these effects and the potential for synergistic treatments to improve outcomes for individuals suffering from neuropathy.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115537"},"PeriodicalIF":2.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective effect of nano-carboxymethyl chitosan from Doryteuthis sibogae against rotenone-induced Parkinson’s disease in the zebrafish model","authors":"Srinivasan Palaniselvam ,&nbsp;Vignesh Narasimman ,&nbsp;R. Vijayashree ,&nbsp;Saravanan Ramachandran","doi":"10.1016/j.bbr.2025.115523","DOIUrl":"10.1016/j.bbr.2025.115523","url":null,"abstract":"<div><div>This study investigates the neuroprotective effects of N-carboxymethyl chitosan (N-CMC) against rotenone (ROT)-induced Parkinson's disease (PD) in a zebrafish (ZF) model. In vivo experiments revealed that ROT-exposed ZF larvae exhibited reduced locomotor activity, increased edge preference, and impaired touch response, while N-CMC treatment significantly improved these behavioral parameters. The reactive oxygen species (ROS) levels in ROT-exposed larvae were elevated (37.75 %) but decreased with N-CMC treatment (30.28 %). Apoptosis was also reduced from 38.87 % in ROT-exposed larvae to 16.52 % with N-CMC treatment. In vitro studies using the N2A cell line confirmed N-CMC’s neuroprotective effects. In adult ZF, ROT exposure decreased locomotion, and N-CMC treatment reversed these effects, as demonstrated through ToxTrac analysis. Novel Tank and Light/Dark tests showed significant behavioral improvements with N-CMC. Neurotransmitter analysis indicated increased dopamine, GABA, and glutamate levels in ROT-exposed ZF, which were moderated with N-CMC treatment. Gene expression analysis of gap43, syn2a, and tuba1b showed upregulation in ROT-exposed ZF, while N-CMC treatment downregulated these genes. Antioxidant assays demonstrated that ROT decreased SOD, CAT, and GSH levels in the brain, while N-CMC treatment increased these antioxidant levels by 1.3-fold, 7.5-fold, and 1.3-fold, respectively. Histopathology revealed neuronal degeneration in ROT-exposed ZF brains, but N-CMC treatment protected the neuronal loss. This study is the first to explore the neuroprotective and antioxidant properties of N-CMC in a ZF model, indicating its potential therapeutic benefits over conventional ROT-based treatments for PD.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115523"},"PeriodicalIF":2.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between neuropsychiatric disorders and aging: A review on telomere length, oxidative stress, and inflammation","authors":"Tala Solh ,&nbsp;Şule Coşkun Cevher","doi":"10.1016/j.bbr.2025.115528","DOIUrl":"10.1016/j.bbr.2025.115528","url":null,"abstract":"<div><div>Aging is the group of time-independent changes that occur in an organism and that ultimately end in death. The relationship between aging and neuropsychiatric disorders is complex. Not only does the incidence of several neuropsychiatric disorders rise with age, but also these disorders are linked with premature mortality and are even thought to be syndromes of accelerated biological aging. Oxidative stress, inflammation and telomere length are factors commonly used to assess biological aging. The purpose of this review is to sum up the existing information about the state of those factors in schizophrenia, depression, bipolar disorder and anxiety disorders, and to summarize the effects of treatment on telomere length in patients with those neuropsychiatric disorders. The main focus, however, is on telomere length seeing the highly controversial study results on this biomarker in neuropsychiatric disorders. There is no scientific consensus on the state of those factors in the mentioned neuropsychiatric disorders or on the effects of treatment on telomere length, thus further research is needed where confounding variables are controlled. Regarding telomere length, it is highly important to explore whether short telomeres lead to the development of neuropsychiatric disorders or vice versa, as it carries huge clinical potential.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115528"},"PeriodicalIF":2.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the neuropeptide S receptor 1 rs324981 polymorphism in modulating emotionality and cognitive flexibility: Insights from a gene-edited mouse model","authors":"Virginie Rappeneau ,&nbsp;Ann-Marie Tobinski ,&nbsp;Leonardo Miguel Caisachana Guevara ,&nbsp;Neele Meyer ,&nbsp;Kay Jüngling ,&nbsp;Chadi Touma","doi":"10.1016/j.bbr.2025.115530","DOIUrl":"10.1016/j.bbr.2025.115530","url":null,"abstract":"<div><div>The neuropeptide S (NPS) and its receptor (NPSR1) are involved in various physiological processes, including arousal, sleep, anxiety, memory, and stress responses in rodents. Recent attention has focused on the association between the NPS/NPSR1 system and stress-related disorders, particularly involving a specific single nucleotide polymorphism (SNP) in the <em>NPSR1</em> gene (rs324981). This SNP causes an amino acid change at position 107 in the protein, reducing NPSR1 signalling potency; however, its effects on behavioural, cognitive, and physiological aspects relevant to stress-related disorders remain unclear.</div><div>Addressing this topic, we characterized the behavioural phenotype of a gene-editing mouse model, expressing either the murine/ancestral NPSR1-I107 variant or the human NPSR1-N107 variant. Both, male and female mice underwent a comprehensive behavioural test battery assessing arousal, exploratory and anxiety-related behaviour under varying levels of novelty stress. Moreover, cognitive functions were evaluated with a special focus on cognitive flexibility using the Attentional Set Shifting Task (ASST). Additionally, markers of behavioural and endocrine stress reactivity were assessed as well as changes in body weight and body composition.</div><div>Our results showed that NPSR1-N107 mice displayed increased anxiety-related behaviour compared to NPSR1-I107 mice, with no significant differences in arousal, exploratory behaviour or hormonal stress responses. However, NPSR1-N107 mice also exhibited better rule-reversal learning in the ASST, indicating enhanced cognitive flexibility. These findings provide clear evidence for a role of the NPSR1 rs324981 SNP in regulating emotionality and cognitive flexibility, underscoring the potential of the NPSR1-I107N mouse model for further elucidating the molecular mechanisms underlying stress-related disorders.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115530"},"PeriodicalIF":2.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"White matter tract involvement in anarchic hand syndrome following stroke: Diffusion tensor imaging study","authors":"Jihee Park ,&nbsp;Woo-Suk Tae ,&nbsp;Sekwang Lee ,&nbsp;Sung-Bom Pyun","doi":"10.1016/j.bbr.2025.115529","DOIUrl":"10.1016/j.bbr.2025.115529","url":null,"abstract":"<div><div>Anarchic Hand Syndrome (AHS) is a rare neurological disorder characterized by involuntary, purposeful hand movements. AHS can also result from damage to the corpus callosum (CC), intra-hemispheric tracts, or descending tracts, but its precise causes remain unclear. This study aimed to identify the white matter tracts associated with AHS development using the automated reconstruction of 42 tracts from diffusion tensor imaging (DTI).</div><div>We included three female AHS patients with anterior cerebral arterial (ACA) infarctions. Additionally, we enrolled 20 age-matched control subjects and six patients with ACA infarctions but without AHS symptoms (N-AHS group) for comparison. DTI was conducted in AHS, N-AHS, and control groups. Fractional anisotropy (FA) and volume values were extracted from the DTI datasets of participants using TRActs Constrained by UnderLying Anatomy (TRACULA) technique.</div><div>The AHS group showed lower FA values of the CC body (parietal and temporal section), right arcuate fasciculus (AF), corticospinal tract, extreme capsule, inferior longitudinal fasciculus (ILF), and superior longitudinal fasciculus than the control group. However, these tracts exhibited no significant difference between N-AHS and control groups. Similarly, the volume value in the genu of CC in the AHS group was lower than controls, but not in other tracts.</div><div>Our results suggests that the extensive CC lesion, especially in the posterior parietal and temporal section of CC body, and damage to intra-hemispheric tracts (AF and ILF), is associated with the development of AHS. These results involved in AHS and underscore the significance of considering a more complex network disruption, involving various white matter tracts beyond CC for understanding this syndrome.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115529"},"PeriodicalIF":2.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic Zebrafish Irritant-evoked Hyperlocomotion (EZIH) as a high-throughput behavioral model for nociception","authors":"Amelia A. Bunnell ,&nbsp;Erin M. Marshall ,&nbsp;Summer K. Estes ,&nbsp;Monica C. Deadmond ,&nbsp;Sandra Loesgen ,&nbsp;James A. Strother","doi":"10.1016/j.bbr.2025.115526","DOIUrl":"10.1016/j.bbr.2025.115526","url":null,"abstract":"<div><div>Behavioral models have served a key role in understanding nociception, the sensory system by which animals detect noxious stimuli in their environment. Developing zebrafish (<em>Danio rerio</em>) are a powerful study organism for examining nociceptive pathways, given the vast array of genetic, developmental, and neuroscience tools available for these animals. However, at present there are few widely-adopted behavioral models for nociception in developing zebrafish. This study examines the locomotor response of hatching-stage zebrafish embryos to dilute solutions of the noxious chemical and TRPA1 agonist allyl isothiocyanate (AITC). At this developmental stage, AITC exposure induces a robust uniphasic hyperlocomotion response. This behavior was thoroughly characterized by determining the effects of pre-treatment with an array of pharmacological agents, including anesthetics, TRPA1 agonists/antagonists, opioids, NSAIDs, benzodiazepines, SSRIs, and SNRIs. Anesthetics suppressed the response to AITC, pre-treatment with TRPA1 agonists induced hyperlocomotion and blunted the response to subsequent AITC exposures, and TRPA1 antagonists and the opioid buprenorphine tended to reduce the response to AITC. The behavioral responses of zebrafish embryos to a noxious chemical were minimally affected by the other pharmacological agents examined. The feasibility of using this behavioral model as a screening platform for drug discovery efforts was then evaluated by assaying a library of natural product mixtures from microbial extracts and fractions. Overall, our results indicate that irritant-evoked locomotion in embryonic zebrafish is a robust behavioral model for nociception with substantial potential for examining the molecular and cellular pathways associated with nociception and for drug discovery efforts.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"485 ","pages":"Article 115526"},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}