Behavioural Brain Research最新文献

{"title":"Region-specific cognitive effects of HD-tDCS in older adults: M1, DLPFC, and cerebellum","authors":"Roya Khanmohammadi,&nbsp;Mehrnaz Inanlu,&nbsp;Vahid Rafiee Manesh","doi":"10.1016/j.bbr.2025.115571","DOIUrl":"10.1016/j.bbr.2025.115571","url":null,"abstract":"<div><div>While regions like the primary motor cortex (M1), left dorsolateral prefrontal cortex (DLPFC), and cerebellum are linked to cognitive functions, it is unclear which offers the greatest cognitive benefit. This study aimed to assess the effects of high-definition transcranial direct current stimulation (HD-tDCS) on these regions, focusing on inhibitory control, cognitive flexibility, and working memory. This parallel, randomized, double-blinded, and controlled trial involved 80 older adults, randomly assigned to one of four groups: anodal stimulation of M1, left DLPFC, cerebellum, or sham. Inhibitory control was assessed using reaction time (RT) and rate-correct score (RCS) from a Go/No-Go task. Trail Making Test A (TMT-A) and B (TMT-B) measured processing speed and cognitive flexibility, while the backward digit span test evaluated working memory. All assessments were conducted pre- and post-stimulation. Notably, cerebellum stimulation significantly improved working memory (p = 0.010), whereas M1, DLPFC, and sham did not. Significant interaction effects emerged for TMT-A and TMT-B, with both M1 and DLPFC stimulation enhancing performance (TMT-A: p = 0.005, p = 0.025; TMT-B: p &lt; 0.001, p = 0.045, respectively), while cerebellum and sham had no significant impact. Additionally, RT and RCS showed no significant effects. Anodal stimulation of M1 and DLPFC improved cognitive flexibility and processing speed, whereas cerebellum stimulation selectively enhanced working memory. However, inhibitory control did not improve, highlighting the need for further tailored interventions. These findings underscore distinct region-specific effects of tDCS on cognitive performance in older adults.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"486 ","pages":"Article 115571"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolonged Drinking in the Dark in Adult Female Mice Attenuates the Central and Peripheral Cytokine Response to Lipopolysaccharide.","authors":"Lauren G Singleton, Victoria A Macht, Rachel A Kohman","doi":"10.1016/j.bbr.2025.115557","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115557","url":null,"abstract":"<p><p>Alcohol misuse has long-term effects on immune function, and can alter the inflammatory profile basally and following an insult. Additionally, alcohol-induced inflammation may promote alcohol cravings and dependence, suggesting a bi-directional relationship. However, most studies assessing the relationship between alcohol consumption and innate immune signaling were performed exclusively in males or yielded ambiguous results. The present study extended this work by assessing the bidirectional association between alcohol and inflammation in the Drinking in the Dark (DID) binge paradigm in adult female mice. A week after receiving LPS (1mg/kg) or saline, female C57BL/6J mice were evaluated for voluntary EtOH consumption in a prolonged DID paradigm. Next, whether prolonged voluntary EtOH intake influenced the inflammatory response to LPS in the brain and liver was assessed. Our results demonstrate that voluntary binge drinking in the DID model was unaltered by a prior immune challenge. However, prolonged alcohol consumption attenuated the central and peripheral immune response to LPS exposure. Compared to female mice who drank only water, EtOH-consuming females showed diminished brain levels of interleukin-1β (IL-1β) (p =0.005) and liver levels of IL-1β and tumor necrosis factor-α four hours after LPS (p < 0.05). Twelve days of DID was not sufficient to promote a basal increase in inflammatory cytokines. Collectively, the results demonstrate that prolonged voluntary EtOH exposure blunts the inflammatory response in adult females. These findings provide novel insight into the immunomodulatory effects of binge alcohol intake in females, contributing to our understanding of the role of inflammatory signaling in alcohol-consuming individuals.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115557"},"PeriodicalIF":2.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Clues to Memory Loss: High-Fat Diets and Brain-Adipose Crosstalk in Zebrafish.","authors":"Farah Mejbel Al Jaberi, Ragdha Alzarzour, Aidiahmad Dewa, Azira Muhamad, Fauziahanim Zakaria","doi":"10.1016/j.bbr.2025.115559","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115559","url":null,"abstract":"<p><p>Obesity is a growing public health concern that significantly impacts cognitive functions, including memory. This research explores how a high-fat diet affects short-term memory, employing the novel object recognition (NOR) test and NMR-based metabolomics to elucidate metabolic alterations in the brain and adipose tissue. The zebrafish were divided into two groups: one receiving a standard diet (SD) and the other a high-fat diet (HFD). Body mass index (BMI) was assessed every two weeks for a period of eight weeks. The NOR test was used to determine the discrimination index (DI) for evaluating the short-term memory of the SD and HFD groups. NMR spectroscopy was employed to investigate the metabolites in brain and adipose tissues, and multivariate data analysis was conducted to discover significant metabolic alterations. The high-fat diet (HFD) resulted in a significant increase in body mass index (BMI) (p < 0.0001) compared to the standard diet (SD) group from week 4 to week 8. A significant reduction in the discrimination index (24.95%) in the HFD group against the SD group suggests a decline in memory performance among HFD subjects. NMR-based metabolomics of adipose tissue revealed that linoleic acid and caprylic acid were consistently found to exhibit increased levels in the HFD group across all assessments, whereas lauric acid, ALA, EPA, and DHA were consistently present at elevated levels in the adipose tissue of the SD group. NMR-based metabolomics of the brain identified GABA, taurine, and histamine as the key metabolites distinguishing the HFD from the SD group in female zebrafish. For male zebrafish brains, taurine, phenylalanine, and tryptophan were identified as the most significant metabolites for differentiating between HFD and SD. These metabolites demonstrated a notable decrease in the HFD group relative to the SD group. The results of this study align with those of previously reported studies in rodents and humans, indicating that memory impairment associated with obesity may stem from neuroinflammation and changes in synaptic plasticity. This research provides insights into the molecular changes in adipose tissue and the brain that occur when individuals receive a high-fat diet (HFD), which may enhance our understanding of the link between obesity and memory impairment, ultimately leading to a better comprehension of the disease.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115559"},"PeriodicalIF":2.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neonatal vocalization rate predicts future prosocial behavior in C57 BL/6J mice","authors":"Matthew S. Binder,&nbsp;Elise B. Cauley,&nbsp;Nicole I. Cofsky,&nbsp;Morgan O. Lemler","doi":"10.1016/j.bbr.2025.115560","DOIUrl":"10.1016/j.bbr.2025.115560","url":null,"abstract":"<div><div>Neonatal Ultrasonic vocalizations (USVs) are an innate form of mouse communicative behavior that are produced throughout the first two postnatal weeks. While neonatal USVs are commonly assessed, their relationship to future behaviors is largely unknown. In the present study, we addressed this by analyzing vocalizations in C57BL/6 pups throughout development. We then examined each animal’s anxiety, locomotion, depressive, prosocial, and aggressive behaviors in adolescence. To analyze the results, we used correlations and also divided the mice into a high and a low group according to quantitative measures of their vocalizations, using a median split design. For call rate, we found a large positive correlation between call rate and sociability, furthermore, high vocalizers were significantly more prosocial than low vocalizers. No other significant differences and significant correlations were found. When we controlled for the relative contribution of the weight, sex, litter size, and sex composition of the litter, as well as the duration, pitch and amplitude of the calls, we found that high vocalizers were still significantly more prosocial than low vocalizers, indicating that this relationship cannot be attributed to these other factors. When the data was split according to the pitch, duration, and amplitude of the vocalizations, no significant adolescent behavioral differences nor correlations were found. Similarly, the types of calls produced had minimal relevance to adolescent behaviors. Altogether, our study elucidated a long-term implication for USVs, finding that the number of USVs produced throughout early development is a significant predictor of an animal’s future prosocial behavior.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"486 ","pages":"Article 115560"},"PeriodicalIF":2.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of reward learning on inhibitory control in internet gaming disorder: Evidence from behavioral and ERP","authors":"Ziyu Mao ,&nbsp;Jing Huang ,&nbsp;Mengyue Zhang ,&nbsp;Meng Zhang ,&nbsp;Chenyue Zhao ,&nbsp;Zhengxing Liu ,&nbsp;Xiaoli Xing","doi":"10.1016/j.bbr.2025.115558","DOIUrl":"10.1016/j.bbr.2025.115558","url":null,"abstract":"<div><div>Reward dysregulation and deficits in inhibitory control significantly contribute to the development of internet gaming disorder (IGD). While prior research demonstrates that reward history influences individuals' inhibitory control, it remains unclear whether this effect extends to individuals with IGD. The primary aim of this study was to investigate whether individuals with IGD exhibit impairments in reward learning and whether prior reward learning influences their inhibitory control, using both behavioral and event-related potential (ERP) measures. This study first employed a probability selection task to examine potential impairments in reward learning among individuals with IGD. Next, a stop-signal task incorporating reward- and punishment-associated stimuli was used to further investigate the behavioral and electroencephalographic effects of prior reward learning on subsequent inhibitory control. Results revealed that during the reward-learning phase, the IGD group exhibited significantly longer response times than the control group in both the learning and transfer phases. Additionally, the feedback-related negativity amplitude in the IGD group was significantly lower than that in the control group. Conversely, the P3 wave amplitude induced by positive and negative feedback in the IGD group were significantly higher than in the control group. In the inhibitory control phase following reward learning, the Nogo-P3 wave amplitude in response to reward cues was significantly greater in the IGD group than in the control group. Moreover, within the IGD group, the Nogo-P3 wave amplitude evoked by reward cues was significantly larger than the amplitude evoked by loss cues. These findings suggest that reward learning is impaired in individuals with IGD and that stimuli with a prior reward history may compromise inhibitory control, potentially serving as a critical factor in addiction development in this population.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"486 ","pages":"Article 115558"},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroanatomical basis of 5-HT1A receptor agonism in disruption of maternal behavior in rats","authors":"Lanlan Zhang ,&nbsp;Jinyue Pang ,&nbsp;Qiyan Feng,&nbsp;Jinmei Hao,&nbsp;Xin Gu,&nbsp;Xiayang Jiang,&nbsp;Shengmei Yang,&nbsp;Wanhong Wei,&nbsp;Ruiyong Wu","doi":"10.1016/j.bbr.2025.115554","DOIUrl":"10.1016/j.bbr.2025.115554","url":null,"abstract":"<div><div>The acute activation of serotonin 1 A (5-HT_1A) receptors appears to disrupt maternal behavior in rats; however, the underlying neuroanatomical mechanisms remain poorly understood. We employed two approaches to investigate the role of 5-HT_1A receptors in maternal behavior to address this knowledge gap. First, we used real-time polymerase chain reaction (PCR) to analyze 5-HT_1A receptor mRNA expression in female rats at different reproductive stages. We identified stage- and region-specific expression patterns, including temporary increases in the nucleus accumbens (NAc), ventral tegmental area (VTA), and dorsal raphe nucleus (DRN), as well as a temporary decrease in the medial prefrontal cortex (mPFC), amygdala, hippocampus, and ventromedial hypothalamic nucleus (VMH) during the perinatal, early, and middle postpartum periods. These findings suggest that coordinated 5-HT_1A activity across these brain regions is critical for normal maternal behavior. Second, we used c-Fos immunohistochemistry to elucidate the central mechanisms underlying the effects of the acute and repeated administration of 8-OH-DPAT (a 5-HT_1A receptor agonist, 1.0 mg/kg, sc.) on maternal behavior. Acute 8-OH-DPAT administration disrupted maternal behaviors, including pup retrieval, pup licking, nest building and hovering over pups, while simultaneously increased c-Fos expression in the mPFC, ventral bed nucleus of the stria terminalis (vBNST), NAc shell, lateral septum (LS), and medial amygdala (MeA). Disruptions in pup retrieval, pup licking and nest building persisted following five days of repeated 8-OH-DPAT treatment, whereas hovering over pups showed substantial recovery, returning to near-normal levels. Concurrently, c-Fos expression increased in the vBNST but decreased in the mPFC, MeA, and DRN. These results suggest that acute and repeated 8-OH-DPAT administration disrupts maternal behavior via distinct presynaptic and postsynaptic 5-HT_1A receptor mechanisms. This study highlights the complex regulatory role of 5-HT_1A receptor activity in maternal care and provides insights into the neuroanatomical and neurochemical mechanisms underlying maternal behavior.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"486 ","pages":"Article 115554"},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do snakes alter our visual attention to food?","authors":"Songhe Li, Justin R Keene, Breanna N Harris, James A Carr","doi":"10.1016/j.bbr.2025.115550","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115550","url":null,"abstract":"<p><p>The need to detect and avoid predators drives many aspects of foraging behavior. Snakes are historical predators of primates, but predator avoidance-foraging tradeoffs are rarely studied in humans. We examined whether the humans have a detection bias for snake versus food images using eye-tracking technology in 76 undergraduate student participants (38 men, 38 women). We tested three questions: 1) Do humans exhibit a visual bias to snakes over food? 2) Does food palatability affect any visual bias to snakes? 3) Is the response to snakes specific for these predators or a generalized reaction to a visually evocative stimulus? We analyzed three metrics in balanced pairs of food and snake images and images normatively ranked for arousal (low, high) and emotional valence (negative and positive): saccade latency, gaze duration, and saccade bouts. There was a strong bias toward shorter saccade latency with the snake images relative to food images. Gaze duration and saccade bouts were significantly greater for snake images relative to food images. Food palatability had discrete effects on the visual bias to snake images. Finally, qualitatively similar effects on visual bias were observed in response to negative valence, high arousal non-snake images. While humans display a gaze bias to snakes over food, this bias may be related to the negative valence and high arousal linked to snake images rather than key visual features of snakes themselves.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115550"},"PeriodicalIF":2.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of working memory training on cognitive flexibility, dendritic spine density and long-term potentiation in female mice.","authors":"Vasiliki Stavroulaki, Lida-Evmorfia Vagiaki, Orestis Nikolidakis, Maria Zafeiri, Maria E Plataki, Kyriaki Sidiropoulou","doi":"10.1016/j.bbr.2025.115555","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115555","url":null,"abstract":"<p><p>Working memory (WM) is a cognitive ability that allows the short-term maintenance and manipulation of information for goal-directed behavior. The prefrontal cortex (PFC) and the hippocampus (HPC) are two brain regions implicated in WM task performance. Several studies indicate that training in WM (WMT) can enhance performance in various other cognitive tasks. However, our understanding of the neurobiological changes induced by WMT is very limited. Previous work from our lab showed that WMT enhances synaptic and structural plasticity in the PFC and HPC in male mice. In this study, we investigate the effect of WMT on cognitive flexibility and synaptic properties in PFC and HPC in adult female mice. To this end, female adult mice were split into 3 groups: a) mice that remained in their home cage (naïve), b) mice that performed the alternation task in the T-maze (non-adaptive) and c) mice that were trained in the delayed alternation task for 9 days (adaptive). The delayed alternation task was used for WMT. In one cohort, following the delayed alternation task, all mice were tested in the attention set-shifting (AST) task to measure cognitive flexibility, followed by harvesting of the brains for Golgi-Cox staining to study dendritic spine density. Our results showed that in female mice, there were no differences in AST performance among the three groups tested, however, the latency to make a choice was reduced in the adaptive group. With regards to dendritic spine density, no significant differences were identified in PFC while increased dendritic spine density was found in HPC of the adaptive group, compared to the naïve group. In a second cohort, acute brain slices were prepared after completion of the delayed alternation task to investigate the synaptic properties in the PFC and the HPC. Evoked field excitatory post-synaptic potential (fEPSP) recordings were performed in either PFC or HPC brain slices. Our results show that tetanic-induced long-term potentiation (LTP) in the PFC was not different among the three training groups. In the HPC, theta-burst induced LTP was significantly increased in the adaptive group also compared to the non-adaptive and naïve groups. These results reveal both similarities and differences of WMT on performance in the attention set-shifting task, dendritic spine density and LTP in females, compared to males.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115555"},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-dependent behavioral alterations in BALB/c mouse bearing a non-CNS solid tumor.","authors":"Isaias Gutierrez-Leal, Luisa M Onofre-Alvarado, Diana Caballero-Hernández, Ana L Cantu-Ruiz, Moises A Franco-Molina, Ricardo Gomez-Flores, Patricia Tamez-Guerra, Cristina Rodríguez-Padilla","doi":"10.1016/j.bbr.2025.115556","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115556","url":null,"abstract":"<p><strong>Background: </strong>Peripheral tumors can alter the central nervous system activity leading to behavior alterations and cancer-related cognitive impairment (CRCI). Although commonly attributed to anti-cancer treatments, findings of CRCI in newly diagnosed cancer patients suggest that tumors alone may impair brain functions, including working memory and processing speed.</p><p><strong>Methods: </strong>We assessed male and female mice behavior using a novel object recognition and a Y maze test along with the open field and burrowing tests. The tests were performed before and after tumor implantation (subcutaneous murine L5178Y-R lymphoma injection in the posterior hind limb), and through its progression to evaluate mobility, anxiety, motivation recognition, and spatial working memory.</p><p><strong>Results: </strong>Male mice showed deficits in recognition memory, scoring a low novel object time exploration (42.26% in males [p = 0.02] and 50.15% [p = 0.53] in females). Spontaneous alternation was significantly impaired in both male (p = 0.01) and female (p = 0.03) mice. During tumor progression, only female mice showed decreased mobility in indicators such as average speed, mobility rate, and total distance in the open field test, as well as deficient burrowing activity, indicating a lack of motivation or sickness behavior. Our findings suggest that tumor burden is associated with behavioral alterations in a sex-dependent manner in a mouse model of lymphoma.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115556"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-29a-3p Ameliorate Behavioral Deficiency in Hypoxia-Ischemia Brain Damage in Neonatal Mice by inhibiting BTG2.","authors":"Qian Luo, Xiaohui Xing, Yan Song, Bing Gu, Quan Hu, Weiyang Liu, Yilei Xiao, Zhen Wang","doi":"10.1016/j.bbr.2025.115552","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115552","url":null,"abstract":"<p><p>It has been reported that miR-29a-3p played a part in series neurological disorders. However, it remains unclear whether miR-29a-3p participate in the pathological mechanism in hypoxia-ischemia (HI) brain injury. In this study, we detected the change of miR-29a-3p level in the ipsilateral cortex following HI brain injury and found that miR-29a-3p was significantly increased at 3 days in the ipsilateral cortex following HI insult in neonatal mice. Therefore, we further explored the role of miR-29a-3p in HI brain injury and its molecular mechanism. The results showed that miR-29a-3p mimics attenuated and miR-29a-3p antagomir aggravated brain infarction volume at 3 days following HI insult. We further found that overexpression of miR-29a-3p also suppressed apoptosis and neuroinflammation, reduced synaptic loss and prevent HI-induced microglial morphological changes 3 days following HI insult. Neurobehavioral tests revealed that overexpression of miR-29a-3p could improve both short-term and long-term behavioral defects after HI injury. Furthermore, we proved that miR-29a-3p targets B-cell translocation gene 2 (BTG2) and further inhibits the expression of Bax by luciferase reporter assay and qRT-PCR. Moreover, overexpression of miR-29a-3p, by applying liposomes through intranasal route, could also achieve the same therapeutic effect in HI injury. Our data showed that by inhibiting BTG2/Bax, increasing level of miR-29a-3p might serve as a strategy to prevent brain damage and behavioral deficiency in HI.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115552"},"PeriodicalIF":2.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}