Behavioural Brain Research最新文献

{"title":"Machine learning approach to DNA methylation and neuroimaging signatures as biomarkers for psychological resilience in young adults","authors":"Shih-Hsien Lin ,&nbsp;Yen-Hsin Chen ,&nbsp;Meng-Heng Yang ,&nbsp;Chih-Wei Lin ,&nbsp;Andrew Ke-Ming Lu ,&nbsp;Cheng-Ta Yang ,&nbsp;Yun-Hsuan Chang ,&nbsp;Bao-Yu Chen ,&nbsp;Shulan Hsieh ,&nbsp;Sheng-Hsiang Lin","doi":"10.1016/j.bbr.2025.115747","DOIUrl":"10.1016/j.bbr.2025.115747","url":null,"abstract":"<div><div>Psychological resilience is influenced by both psychological and biological factors. However, the potential of using DNA methylation (DNAm) probes and brain imaging variables to predict psychological resilience remains unclear. This study aimed to investigate DNAm, structural magnetic resonance imaging (sMRI), and diffusion tensor imaging (DTI) as biomarkers for psychological resilience. Additionally, we evaluated the ability of epigenetic and imaging markers to distinguish between individuals with low and high resilience using machine learning algorithms. A total of 130 young adults assessed with the Connor–Davidson Resilience Scale (CD-RISC) were divided into high and low psychological resilience groups. We utilized two feature selection algorithms, the Boruta and variable selection using random forest (varSelRF), to identify important variables based on nine for DNAm, sixty-eight for gray matter volume (GMV) measured with sMRI, and fifty-four diffusion indices of DTI. We constructed machine learning models to identify low resilience individuals using the selected variables. The study identified thirteen variables (five DNAm, five GMV, and three DTI diffusion indices) from feature selection methods. We utilized the selected variables based on 10-fold cross validation using four machine learning models for low resilience (AUC = 0.77–0.82). In interaction analysis, we identified cg03013609 had a stronger interaction with cg17682313 and the rostral middle frontal gyrus in the right hemisphere for psychological resilience. Our findings supported the concept that DNAm, sMRI, and DTI signatures can identify individuals with low psychological resilience. These combined epigenetic imaging markers demonstrated high discriminative abilities for low psychological resilience using machine learning models.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115747"},"PeriodicalIF":2.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vulnerability to chronic stress in male rats: Additive effect of low positive affectivity and high hedonic response as measured by sucrose intake","authors":"Marten Vares ,&nbsp;Margus Kanarik ,&nbsp;Kadri Kõiv ,&nbsp;Markus Vares ,&nbsp;Karolina Anja ,&nbsp;Mait Metelitsa ,&nbsp;Karita Laugus ,&nbsp;Helene Tigro ,&nbsp;Sirin Korulu ,&nbsp;Ruth Shimmo ,&nbsp;Jaanus Harro","doi":"10.1016/j.bbr.2025.115749","DOIUrl":"10.1016/j.bbr.2025.115749","url":null,"abstract":"<div><div>Stress contributes to the development of psychiatric disorders. We have previously shown that rats with low inherent positive affectivity, assessed by 50-kHz ultrasonic vocalizations (USV), are more vulnerable to stress, and that free-fed rats with persistently lower consumption of sucrose are less sensitive. Hence we compared the association of these traits with the effect of chronic stress within a single experiment. Male Wistar rats were classified as of high (HC) or low (LC) positive affectivity based on their average 50-kHz USV response, and as young adults the rats were further divided into high and low sucrose-consuming (HSuc and LSuc, respectively). Four groups comprising twenty rats each were formed, and half of the animals submitted to chronic variable stress (CVS) for 5 weeks. CVS was followed by behavioural tests and <em>ex vivo</em> biochemical analyses. In elevated plus-maze, CVS increased the anxiety-related measures most prominently in the LC-HSuc rats. Stress reduced amphetamine-induced 50-kHz USVs statistically significantly only in LC-HSuc rats. Serum glucose and adrenal weight, as well as levels of monoamines and their metabolites in the frontal cortex, striatum, nucleus accumbens, amygdala and hypothalamus, were altered by chronic stress mostly in a phenotype-dependent manner. CVS also increased 5-HT_2 C-receptor gene expression in the striatum of LC-HSuc rats. Conclusively, previous separate findings that individual differences in positive affectivity and sucrose intake (hedonic response) can contribute to stress vulnerability were confirmed. Importantly, the highest vulnerability to stress was found if low positive affectivity and high sucrose consumption coincided.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115749"},"PeriodicalIF":2.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual differences in the activity of executive function brain regions during number comparison","authors":"Amanda Martinez-Lincoln ,&nbsp;Daniel R. Leopold ,&nbsp;Boman R. Groff ,&nbsp;Darren J. Yeo ,&nbsp;Erik G. Willcutt ,&nbsp;Laurie E. Cutting ,&nbsp;Marie T. Banich ,&nbsp;Gavin R. Price","doi":"10.1016/j.bbr.2025.115740","DOIUrl":"10.1016/j.bbr.2025.115740","url":null,"abstract":"<div><div>Math skills require the integration of math-specific and domain-general skills, such as executive functions (EF). Neuroimaging studies consistently report intraparietal sulci activation during arithmetic tasks; however, activation of frontal brain regions associated with EF varies across studies. The discrepancies in brain regions associated with EF during math tasks may be due, in part, to variations amongst individuals and task demands. The current study examined neural activations associated with ratio effect in canonical math and EF regions in adolescents and subsequently examined how this activity was related to concurrently acquired behavioral measures of math ability and EF. Findings revealed differential relations between behavioral measures and neural ratio effects for symbolic (i.e., digits) vs. nonsymbolic (i.e., dot arrays) stimuli. The neural ratio effect during symbolic number comparison in the left inferior parietal lobe (IPL) and left inferior frontal junction (IFJ) correlated positively with an individual’s calculation scores. Similarly, the neural ratio effect during nonsymbolic comparison in the right and left inferior parietal lobes correlated positively with an individual’s math fluency. However, while a measure of an individual’s inhibitory control positively correlated with the nonsymbolic neural ratio effect in the left IFJ, working memory positively correlated with the symbolic neural ratio effect in the left IPL, left IFJ, left precentral gyrus, and left posterior dorsolateral prefrontal cortex. These findings suggest that the format of numerical information influences the neural systems engaged and that engagement varies with individual differences in math abilities.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115740"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTS OF SEROTONIN REUPTAKE INHIBITION ON ANXIETY-LIKE BEHAVIOR AND ADIPOCYTES MORPHOMETRY IN OFFSPRING SUBMITTED TO MATERNAL HIGH-FAT/HYPERCALORIC DIET.","authors":"Diana Isabela Machado Corrêa, Jeymesson Raphael Cardoso Vieira, Luana Olegário da Silva, Raquel da Silva Aragão, Thaynan Raquel Dos Prazeres Oliveira, Regina Katiuska Bezerra da Silva, Kelli Nogueira Ferraz Pereira Althoff, Widarlane Ângela da Silva Alves, Eduardo Carvalho Lira, Isabeli Lins Pinheiro, Lígia Cristina Monteiro Galindo","doi":"10.1016/j.bbr.2025.115746","DOIUrl":"https://doi.org/10.1016/j.bbr.2025.115746","url":null,"abstract":"<p><p>A high-fat/hypercaloric diet (HHD) can reduce the synaptic availability of serotonin (5-HT), an effect opposite to that attributed to Serotonin Reuptake Inhibition (SRI). This study aimed to investigate the effects of SRI via fluoxetine on body weight (BW), anxiety-like behavior, and adiposity in the offspring of rats exposed to maternal HHD.</p><p><strong>Materials and methods: </strong>Rats received Control Diet (C, 3.44kcal/g) or HHD (4.62kcal/g) from before mating until the end of lactation. On the 1^st postnatal day (PND), the litters received sterile saline (S, 10µl/g) or fluoxetine (F, 10mg/Kg, 10µl/g), as follows: C-S; C-F; HHD-S and HHD-F. In young and adult offspring, BW, anxiety-like behavior, and retroperitoneal white adipose tissue (RWAT) were evaluated.</p><p><strong>Results: </strong>From the 10^th to the 60^th PND, the HHD-F and C-F groups presented lower BW than their respective controls. In young offspring, the HHD-F group spent less time in the periphery and less time in immobility than the C-F group, besides less immobility in the HHD-S group. In adult offspring, time in the periphery and immobility were greater in the HHD-F group than in the HHD-S group and the HHD-F group than in the C-F group, respectively. At the 30^th PND, the weight of WAT was lower in the HHD-F group than in the C-F group. At the 60^th PND, the WAT weight was lower in the HHD-F group than in the HHD-S group. The area and perimeter of adipocytes were smaller in HHD-F than in HHD-S at the 30^th and 60^th PND.</p><p><strong>Conclusions: </strong>IRS attenuated the effects of maternal HHD consumption on BW and RWAT and on the area and perimeter of adipocytes in the offspring of rats. However, in the long term, the effects of SRI on behavioral phenotypic expression were less obvious.</p>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":" ","pages":"115746"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term intermittent fasting induces region- and sex-specific changes in astrocyte morphology and expression and anxiety-like behaviors in CD-1 mice","authors":"Minerva Ortiz-Valladares ,&nbsp;Diana Amezcua-Cárdenas ,&nbsp;Ricardo Pedraza-Medina ,&nbsp;Christian Peregrino-Ramírez ,&nbsp;Esmeralda Rojas-Cobián","doi":"10.1016/j.bbr.2025.115745","DOIUrl":"10.1016/j.bbr.2025.115745","url":null,"abstract":"<div><div>Intermittent fasting (IF) is a metabolic intervention that alters systemic and cerebral energy dynamics, promoting neurochemical and cellular adaptations that may influence mood and emotional regulation. Astrocytes, as key regulators of brain metabolism, neurotransmitter clearance, and neuroinflammatory processes, are particularly sensitive to fluctuations in nutrient availability. Dysregulation of astrocytic morphology and glial fibrillary acidic protein (GFAP) expression has been implicated in the pathophysiology of anxiety. However, the long-term effects of IF on astrocyte integrity and behavior remain poorly understood. This study examined how a six-week 16:8 IF protocol, initiated at postnatal day (PD) 60, modulates astrocytic morphology and GFAP expression in brain regions involved in emotional and homeostatic processing, including the medial prefrontal cortex (mPFC), insular cortex (IC), and amygdala (AM), in male and female CD1 mice. Anxiety-like behavior was assessed on PDs 102–103 using the Open Field Test (OFT), Elevated Plus Maze (EPM), and Light-Dark Test (LDT). Immunohistochemistry and Sholl analysis were used to quantify astrocyte number, cytoplasmic area, and branching complexity. IF increased anxiety-like behavior, particularly in the EPM. Region- and sex-specific astrocytic remodeling was observed: IF reduced cytoplasmic area and branching in the mPFC, induced modest changes in the IC, and produced robust increases in GFAP-positive astrocyte density and proximal arborization in the AM. Notably, anxiety indices positively correlated with astrocyte number in the AM, suggesting a potential link between glial reactivity and emotional responses to metabolic stress. These findings highlight the amygdala as a key site of astrocytic sensitivity to IF and underscore the importance of brain region and sex as modulators of glial adaptation.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115745"},"PeriodicalIF":2.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral or intranasal angiotensin-(1-7) improves anxiety and depression-like behaviors in mice subjected to allergic pulmonary inflammation","authors":"Sthéfanie C.A. Gonçalves ,&nbsp;Juliana F. Gregório ,&nbsp;Kamylle S. Ferraz ,&nbsp;Giselle S. Magalhães ,&nbsp;Silvia A. Zebral ,&nbsp;Marco Antônio P. Fontes ,&nbsp;Maria da Gloria Rodrigues-Machado ,&nbsp;Ruben D. Sinisterra ,&nbsp;Andrea S. Haibara ,&nbsp;Lucas M. Kangussu ,&nbsp;Robson A.S. Santos ,&nbsp;Maria Jose Campagnole-Santos","doi":"10.1016/j.bbr.2025.115744","DOIUrl":"10.1016/j.bbr.2025.115744","url":null,"abstract":"<div><div>Allergic asthma is a chronic respiratory disease characterized by airway inflammation and hyperresponsiveness, that has a challenging management when associated with anxiety and depression, which affects around 50 % of patients with difficult-to-control asthma. Angiotensin-(1-7) [Ang-(1-7)], a key mediator of the renin-angiotensin system, has demonstrated the ability to attenuate airway remodeling, hyperreactivity and to promote resolution of lung inflammation. Furthermore, Ang-(1-7) exhibits anxiolytic and antidepressant effects when administered into the brain. Here we investigated whether peripheral administration of Ang-(1-7) by two different routes could alleviate depressive and anxiety-like behaviors and inflammation in asthmatic mice. Male Balb/c mice, 8–10 weeks old, underwent sensitization and challenge with ovalbumin. Asthmatic mice received either an oral or intranasal formulation of Ang-(1-7) included in hydroxypropyl-β-cyclodextrin (HPβCD). Both oral and intranasal Ang-(1-7) treatments induced reduction in lung inflammation, and effectively ameliorated depressive and anxiety-like behaviors, as shown in elevated plus maze, open field, and tail suspension tests. Notably, intranasal Ang-(1-7) decreased levels of TNF-α and IL-6 in the prefrontal cortex, and, regardless of treatment, there was an elevation in IL-10. Additionally, in other asthmatic animals, ICV administration of Ang-(1-7), which resulted in the expected attenuation in depressive and anxiety-like behaviors, did not significantly reduce lung inflammation, suggesting the improvement in behavioral tests following peripheral treatment with Ang-(1-7) cannot be solely attributed to a decrease in pulmonary inflammation. The data of the present study showed that both intranasal and oral administration of Ang-(1-7) not only alleviates pulmonary inflammation but also mitigates stress-coping behaviors, indicating this peptide as a promising therapeutic candidate for enhancing the treatment and quality of life for patients with asthma.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115744"},"PeriodicalIF":2.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regions of interest assessment of prenatal exposure to tobacco on adolescent cortical thickness and sulcal depth","authors":"Andrew Kochvar ,&nbsp;Steven R. Laviolette ,&nbsp;Ali S. Khan ,&nbsp;Benjamin Grin ,&nbsp;Hongying Daisy Dai","doi":"10.1016/j.bbr.2025.115741","DOIUrl":"10.1016/j.bbr.2025.115741","url":null,"abstract":"<div><div>Maternal tobacco use during pregnancy (MTDP) remains a global and domestic public health issue. This study seeks to investigate the long-term impact of MTDP on brain morphology during late childhood and early adolescence using the Adolescent Brain Cognitive Development (ABCD) dataset. Children aged 9–10 were enrolled using the ABCD school selection probability sample method for national representation. Participants and their parents or guardians underwent interviews and surveys, and children underwent Magnetic Resonance Imaging (MRI) at baseline and 2-year follow-up. Morphometric brain measures of cortical thickness and sulcal depth across 34 regions of interest on T1-weighted MRI images were analyzed. Of 11,448 at baseline, 1607 children fell into the MTDP group. Intracranial volume (p &lt; 0.001), total cortical surface area, and volume (p &lt; 0.0001) were significantly lower among MTDP children (vs. control) at both waves 1 and 2. A sustained difference was found in mean cortical thickness at the parahippocampal gyrus as well as sulcal depth at the isthmus cingulate, parahippocampal, lateral occipital, and lingual gyri. Several regions of interest demonstrated differences in the cortical thickness and sulcal depth at single time points. An association between MTDP and long-term outcomes of regional morphometric differences in cortical thickness and sulcal depth on MRI was found at both baseline among 9–10 years old and at 2-year follow-ups. Taken together with NIH cognitive testing from the same population comparison, the results suggest longstanding cognitive deficits corresponding to specific brain regions.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115741"},"PeriodicalIF":2.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Setting standards: Auditory MMN and P3a habituate differentially in a two-syllable oddball task","authors":"Tobias A. Wagner-Altendorf ,&nbsp;Valentina M. Skeries ,&nbsp;Marlitt Rein ,&nbsp;Anna Cirkel ,&nbsp;Thomas F. Münte ,&nbsp;Marcus Heldmann","doi":"10.1016/j.bbr.2025.115743","DOIUrl":"10.1016/j.bbr.2025.115743","url":null,"abstract":"<div><div>The Mismatch Negativity (MMN) and the P3a of the event-related EEG potential (ERP) serve as indices of prediction error detection in processing auditory stimuli. The characteristics of both components regarding the significance of the stimuli as standard or deviant have now been comprehensively described, but little is known about how these components behave during the establishment of standards or deviants. Most investigations used paradigms where the average of many instantiations of a stimulus per participant is calculated. Thus, the nascent process of establishing a stimulus as a standard stimulus, i.e., the very initial process of setting a standard, cannot be tracked. The present study used an auditory oddball task, building on a previous study using a two-tone pitch paradigm, but incorporating the speech syllables “ba” (standard) and “pa” (deviant). A rather small number of stimuli (11 deviant instantiations) was applied to a large sample of participants (&gt; 230), to allow for ERP characterization as a function of the standard’s or deviant’s position in a series of standard and deviant stimuli. We found that, beginning with the fourth instantiation of the standard/deviant pair, the (new) standard was established, and no further modification of the ERP to repeated presentation of the standard stimulus could be detected. Analyzing MMN and P3a components to deviant stimuli revealed a prominent habituation of the P3a between the first and the second instantiation of the deviant, whereas no relevant habituation of the MMN could be detected, indicative of a differential MMN/P3a habituation to repeated speech-like auditory stimuli.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115743"},"PeriodicalIF":2.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The human brain in decision-making processes in hospital administrative and financial units: Neural mechanisms, theta activity, and awareness","authors":"Zeynep Merve Dinler ,&nbsp;Mahmut Akbolat","doi":"10.1016/j.bbr.2025.115737","DOIUrl":"10.1016/j.bbr.2025.115737","url":null,"abstract":"<div><div>This study aims to examine theta activity in the frontal region during rational decisions and in the temporal region during intuitive decisions. It also evaluates awareness levels using a decision-making scale administered to personnel in hospital administrative and financial units. The study included 56 participants (32 women, 24 men). The experimental design employed EEG recordings during decision-making tasks, with theta (4–7 Hz) responses analyzed using oscillatory brain activity methods. The findings indicate a significant increase in theta activity in the frontal region during rational decision-making (F = 21.104, p &lt; 0.001), suggesting that these decisions require greater cognitive control and attention. This supports cognitive control theories that highlight the role of the prefrontal cortex in deliberative processes. Additionally, elevated theta activity was observed in the temporal regions during intuitive decision-making (F = 6.821, p &lt; 0.001), reflecting the involvement of memory and sensory integration processes. Weak negative correlations were found between rational decision-making scale scores and theta activity in the T7 region (r = –.129), and between intuitive decision-making scale scores and theta activity in the T8 region (r = –.180). These findings suggest that self-reported awareness may only partially capture the underlying neurophysiological mechanisms. Overall, these results highlight the role of regional brain oscillations in differentiating rational and intuitive decision-making. They carry important implications for enhancing cognitive assessment tools and advancing our understanding of neural mechanisms in high-stakes hospital settings.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115737"},"PeriodicalIF":2.6,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repeated administration of AMPA/kainate antagonists transiently suppresses nicotine-seeking in an operant rat model","authors":"Maria Hrickova,&nbsp;Petra Amchova,&nbsp;Jana Ruda-Kucerova","doi":"10.1016/j.bbr.2025.115742","DOIUrl":"10.1016/j.bbr.2025.115742","url":null,"abstract":"<div><div>This study evaluated the impact of repeated intravenous administration of AMPA/kainate receptor antagonists, NBQX and CNQX, on the operant nicotine self-administration in rats. Wistar rats were subjected to operant intravenous self-administration of nicotine under a fixed ratio 1 schedule of reinforcement. During the maintenance phase of self-administration, 5 mg/kg intravenous NBQX was administered daily prior to the session for a duration of 10 days. Subsequently, the rats underwent a forced abstinence period, remaining in their home cages for two weeks. Following the abstinence, a reinstatement session was conducted. During the final 3 days of abstinence and preceding the reinstatement session, the animals were treated with either NBQX or CNQX (5 and 6 mg/kg intravenously, respectively) to assess their effect on drug-seeking behavior. A ten-day treatment with NBQX did not have any significant influence on the nicotine intake. Repeated administration of NBQX or CNQX before reinstatement had no effect on nicotine-seeking behavior in terms of total numbers of operant responses. However, both of these ligands decreased the number of active nose-pokes (nicotine-seeking) in the first 20 min of the reinstatement session. While no effect of repeated NBQX treatment on nicotine intake was observed, this study reports a transient effect of repeated NBQX or CNQX treatment in the reinstatement of nicotine-seeking behavior in an operant IVSA model. Further pharmacokinetic studies may help optimize behavioral protocols assessing the potential role of AMPA/kainate antagonists in the field of nicotine addiction research where current evidence remains limited.</div></div>","PeriodicalId":8823,"journal":{"name":"Behavioural Brain Research","volume":"494 ","pages":"Article 115742"},"PeriodicalIF":2.6,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"心理学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}