Biochemical Systematics and Ecology最新文献

{"title":"A survey of tryptamines in southern African Senegalia and Vachellia reveals N,N-dimethyltryptamine in Senegalia ataxacantha","authors":"Nicholas Sadgrove,&nbsp;Ben-Erik Van Wyk","doi":"10.1016/j.bse.2025.104993","DOIUrl":"10.1016/j.bse.2025.104993","url":null,"abstract":"<div><div>As part of a preliminary chemophenetic study of the two genera of African ex. <em>Acacia</em> Mill. <em>sensu lato</em> (<em>Senegalia</em> Raf. and <em>Vachellia</em> Wight &amp; Arn.), the distribution of alkaloids and amines was investigated with a focus on resolving unsubstantiated claims surrounding the existence of psychedelics in these genera. Nineteen of the 44 southern African taxa were screened, including eleven from <em>Senegalia</em> and eight from <em>Vachellia</em>. Lipophilic amine-specific extracts were produced from leaves, including other organs if available, then the extracts were studied by high performance liquid chromatography. The identities of four compounds were determined by nuclear magnetic resonance spectroscopy, i.e., tryptamine, <em>N</em>-methyltryptamine (NMT), <em>N,N</em>-dimethyltryptamine (DMT) and hordenine. With repeated sampling of select species (<em>Senegalia ataxacantha</em> (DC.) Kyal. &amp; Boatwr., <em>Senegalia caffra</em> (Thunb.) P.J.H.Hurter &amp; Mabb., and <em>Senegalia polyacantha</em> (Willd.) Seigler &amp; Ebinger.) it was demonstrated that the chemistry varies intraspecifically, and potentially according to season or organ (flowers, bark, or leaves). It was observed that in South African biota from Gauteng province, tryptamines are detected in species from <em>Senegalia</em>, but not from <em>Vachellia</em> so far, representing a potential chemosystematic marker requiring further research. Only <em>S. ataxacantha</em> had quantifiable levels of the psychedelic amine DMT, occurring in the leaves and flowers at concentrations ranging from 0.01 to 0.05% (mass/mass, wet leaf weight). Quantifiable levels of DMT were detected in just one specimen from a single population, but not in others, or other populations sampled (aside from trace amounts). We conclude that further research is required to identify southern African genotypes of <em>Senegalia</em> and <em>Vachellia</em> with levels of small lipophilic amines and alkaloids high enough to be compared to the species used for psychedelic purposes in South America (1.0 &gt; 0.2% of mass, wet leaf weight) and to identify quantifiable levels of tryptamines (&gt;0.01% of mass, wet leaf weight) in species of <em>Vachellia</em>. We also emphasize that due to geographical chemical variation, our data cannot be compared to other parts of Africa, such as Egypt or other countries in eastern Africa.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104993"},"PeriodicalIF":1.4,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new ceramide and other constituents from Crinum ornatum (L.f.) Herb. (Amaryllidaceae) and their chemophenetic significance","authors":"Kedeu Kouvourbe ,&nbsp;Christine Claire Waleguele ,&nbsp;Yves Martial Mba Nguekeu ,&nbsp;Elise Vanessa Ngouadjio Temgoua ,&nbsp;Elodie Gaële Matheuda ,&nbsp;Marie Germaine Tsepeupon Matchide ,&nbsp;Marthe Aimée Tchuente Tchuenmogne ,&nbsp;Arno Rusel Donfack Nanfack ,&nbsp;Takeshi Kodama ,&nbsp;Stijn Anthonissen ,&nbsp;Wim Dehaen ,&nbsp;Silvère Augustin Ngouela ,&nbsp;Mathieu Tene ,&nbsp;Morita Hiroyuki ,&nbsp;Maurice Ducret Awouafack","doi":"10.1016/j.bse.2025.104991","DOIUrl":"10.1016/j.bse.2025.104991","url":null,"abstract":"<div><div>A previously undescribed ceramide, crinceramide (<strong>1</strong>), along with nineteen known compounds (<strong>2</strong>–<strong>20</strong>) were isolated from <em>Crinum ornatum</em> (L.f.) Herb. using column chromatography techniques over silica gel and Sephadex LH-20. The structure of the new compound was established by different spectroscopic methods including 1D and 2D-NMR, HRESI-MS and chemical conversions. The extract and fractions had strong activities on three human cancer cells (lung A549, breast MCF7, and cervical HeLa) with IC₅₀ from &lt;0.63 to 9.76 μg/mL whereas compounds displayed IC₅₀ ranging from 39.79 to &gt; 100 μM. Moupinamide (<strong>2</strong>) was the most active with IC₅₀ 39.79 ± 0.47 μM on A549 cell and <strong>1</strong> had IC₅₀ &gt; 100 μM against all cells used. The antibacterial activity was also assayed and moupinamide (<strong>2</strong>) had significant activity against <em>Mycobacterium smegmatis</em> (MIC = 9.7 μg/mL) while other samples possessed mostly weak and moderate activities. To the best of our knowledge, this study is the first isolation of ceramide from the <em>Crinum</em> genus, providing new insights into the chemodiversity of this genus. Moreover, new data concerning the pharmacological properties of <em>C. ornatum</em> (L.f.) Herb. and its chemophenetic significance in the genus <em>Crinum</em> and the Amaryllidaceae family were revealed.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104991"},"PeriodicalIF":1.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mulberry Diels-Alder-type adducts and other phenolic compounds with ATP-citrate lyase inhibitory effects from Morus alba and their chemotaxonomic significance","authors":"Qi Zhou,&nbsp;Ying-Peng Tong,&nbsp;Jiang Wan,&nbsp;Li-Hong Yan,&nbsp;Chun-Xiao Jiang,&nbsp;Jin-Feng Hu","doi":"10.1016/j.bse.2025.104992","DOIUrl":"10.1016/j.bse.2025.104992","url":null,"abstract":"<div><div>A phytochemical investigation on the twigs of <em>Morus alba</em> L. (family Moraceae) resulted in the isolation of 13 compounds (<strong>1</strong>−<strong>13</strong>), including five mulberry Diels-Alder-type adducts (MDAAs, <strong>1</strong>−<strong>5</strong>), two 2-arylbenzofurans (<strong>6</strong>, <strong>7</strong>), two flavonoids (<strong>8</strong>, <strong>9</strong>), one biphenyl (<strong>10</strong>), one lignan (<strong>11</strong>), and two coumarins (<strong>12</strong>, <strong>13</strong>). Among them, lyoniresinol (<strong>11</strong>) was obtained from the genus <em>Morus</em> for the first time. The structures of these compounds were determined by comparison of their physical and spectroscopic data with those reported in the literature. Kuwanon J (<strong>1</strong>), mulberrofuran J (<strong>2</strong>), kuwanon G (<strong>3</strong>), albafuran C (<strong>4</strong>), mulberrofuran G (<strong>5</strong>), mulberroside C (<strong>6</strong>), and oxyresveratrol (<strong>10</strong>) exhibited remarkable inhibitory effects against ATP-citrate lyase, with IC₅₀ values ranging from 0.3 to 14.9 μM. The chemotaxonomic significance of the isolated MDAAs and other phenolic compounds within the <em>Morus</em> species was discussed through a compound-species network analysis.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104992"},"PeriodicalIF":1.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new aggreceride derivatives and other chemical constituents from the stems of Tarenna conferta Benth and their potential antileishmanial activity against Leishmania donovani promastigotes: Insight from molecular docking analysis","authors":"Raïssa Carelle Dzukoug ,&nbsp;Angelique Nicolas Messi ,&nbsp;Rostanie Dongmo Zeukang ,&nbsp;Susan Lucia Bonnet ,&nbsp;Anke Wilhelm ,&nbsp;Denis-Gregoire Tene ,&nbsp;Xavier Siwe-Noundou ,&nbsp;Abderaman Bintou Souham ,&nbsp;Karel Grobler von Eschwege ,&nbsp;Alex de Theodore Atchade ,&nbsp;Joséphine Ngo Mbing ,&nbsp;Dieudonné Emmanuel Pegnyemb ,&nbsp;Anastasie Ewola Tih ,&nbsp;Raphaël Tih Ghogomu ,&nbsp;Muhammad Iqbal Choudhary","doi":"10.1016/j.bse.2025.104990","DOIUrl":"10.1016/j.bse.2025.104990","url":null,"abstract":"<div><div><em>Tarenna conferta</em> Benth is traditionally used to treat fever, headaches, parasitic and skin diseases. The aim of this study was to identify potential antileishmanial bioactive compounds from <em>T. conferta</em> and <em>in silico</em> studies against target receptors (2JK6 and 2W0H). The performance of successive chromatographic techniques followed by characterization using NMR (1D and 2D) as well as HR-ESIMS analyses, of methylene choride fraction of the stems of <em>T. conferta</em>, led to the identification of two unprecedented derivatives i.e. a new derivative of a dicarboxylic acid (<strong>1</strong>) and a new hydroxy ester derivative (<strong>2</strong>), with seven known compounds <strong>3</strong>, <strong>4</strong>, <strong>5</strong>, <strong>6</strong>, <strong>7</strong>, <strong>8</strong> and <strong>9</strong>. Compounds <strong>1, 3</strong> and <strong>4</strong> displayed highly potent antileishmanial activity against <em>Leishmania donovani</em> promastigotes with IC₅₀ values of 3.586 ± 0.554 μg/mL, 0.154 ± 0.8123 μg/mL and 0.789 ± 0.105 μg/mL respectively, compared to amphotericine B (IC₅₀ = 0.198 ± 0.704 μM), used as positive control. However crude extract exhibited significant antileishmanial activity against <em>L. donovani</em> (MHOM/SD/62/1S) promastigotes (IC₅₀ 56.860 ± 1.755 μg/mL) and showed no cytotoxicity on RAW 264.7 macrophage cells. The molecular docking provides a captivating glimpse into the intricate interactions between compounds <strong>1</strong>, <strong>2</strong>, <strong>3</strong>, and <strong>4</strong> and the target receptors (2JK6 and 2W0H). The results revealed that, compounds <strong>1</strong>, <strong>3</strong>, and <strong>4</strong> consistently displayed strong binding affinities and hydrogen bond interactions, showcasing their potential for effective therapeutic interventions. Compounds <strong>2</strong> and <strong>3</strong> exhibit moderate binding affinities with essential amino acids. In terms of potential drug development, compound <strong>4</strong> emerges as a standout candidate due to its exceptionally high binding affinities and specific interactions.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104990"},"PeriodicalIF":1.4,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and chemotaxonomic studies on the roots of Angelica acutiloba","authors":"Zhi-Xia Sun ,&nbsp;Fei-Fei Geng ,&nbsp;Han-Yu Zhang ,&nbsp;Yong-Hu Chen ,&nbsp;Zhe Jiang ,&nbsp;Xue-Zheng Li","doi":"10.1016/j.bse.2025.104985","DOIUrl":"10.1016/j.bse.2025.104985","url":null,"abstract":"<div><div>A chemical study of <em>Angelica acutiloba</em> resulted in the isolation of 27 distinct compounds: six phthalides (<strong>1</strong>–<strong>6</strong>), four coumarins (<strong>7</strong>–<strong>10</strong>), one benzofuran (<strong>11</strong>), five esters (<strong>12–16</strong>), one saturated fatty acid (<strong>17</strong>)<strong>,</strong> five phenols (<strong>18–22</strong>), one sterol (<strong>23</strong>), one amide (<strong>24</strong>), one hydroxy acid (<strong>25</strong>), two glycosides (<strong>26, 27</strong>). Among these, several compounds were isolated from various sources for the first time. Specifically, compounds <strong>4</strong>, <strong>13</strong>, <strong>16</strong>, <strong>20</strong>, and <strong>25</strong> were isolated for the first time from the family Apiaceae; compounds <strong>2</strong>, <strong>3</strong>, <strong>5</strong>, <strong>7</strong>, <strong>10</strong>, <strong>12</strong>, <strong>21</strong>, <strong>22</strong>, and <strong>26</strong> were isolated for the first time from the genus <em>Angelica</em> L.; and compounds <strong>6</strong>, <strong>8</strong>, <strong>9</strong>, <strong>11</strong>, <strong>14</strong>, <strong>18</strong>, <strong>19</strong>, <strong>24</strong>, and <strong>27</strong> were isolated for the first time from <em>A. acutiloba</em>. In addition, the chemotaxonomic importance of the compounds isolated from <em>A. acutiloba</em> were addressed. The results demonstrated that <em>A. acutiloba</em> comprises a range of typical components observed in the family Apiaceae and exhibits a close genetic relationship with <em>A. sinensis</em>.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104985"},"PeriodicalIF":1.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variation in chemical composition and bioactivity of Eucalyptus tereticornis leaf essential oil","authors":"Mamta Khaiper ,&nbsp;Pawan Kumar Poonia ,&nbsp;Sunil Kumar Dhanda ,&nbsp;Ravi Beniwal ,&nbsp;Preety Verma ,&nbsp;Mohammed Nasir","doi":"10.1016/j.bse.2025.104988","DOIUrl":"10.1016/j.bse.2025.104988","url":null,"abstract":"<div><div><em>Eucalyptus</em> is recognized worldwide for its medicinal properties. This study aimed to evaluate the chemical composition, antifungal and antioxidant properties of essential oils (EOs) from <em>Eucalyptus tereticornis</em> clone C-7 during different seasons: winter, summer and rainy. EOs were obtained from leaves via hydrodistillation and their chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). The analysis identified 77, 57 and 82 compounds in the EOs obtained during winter, summer and rainy season, respectively. The key constituents identified included isopulegol, eucalyptol, <em>cis</em>-sabinene hydrate, camphene, 3-carene, bicyclogermacrene and (−)globulol. Summer season EOs showed the most potent antifungal activity against the four fungal species tested: Magnaporthe grisea, Rhizoctonia solani, Rhizoctonia bataticola and Fusarium oxysporum. Furthermore, the antiradical activity by DPPH test was highest in summer season EOs, followed by rainy season EOs. The seasonal variation in the chemical composition of <em>Eucalyptus tereticornis</em> EOs highlights that its potential use should consider its harvesting for medicinal and nutraceutical applications.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104988"},"PeriodicalIF":1.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urease inhibitory compounds from the stem bark of Parinari excelsa sabine and their chemophenetic significance","authors":"Alain W. Ngouonpe ,&nbsp;Joseph Tchamgoue ,&nbsp;Alain Tadjong Tcho ,&nbsp;Bruno Dupon A. Ambamba ,&nbsp;Muhammad S. Ali ,&nbsp;Mehreen Lateef ,&nbsp;Simeon F. Kouam","doi":"10.1016/j.bse.2025.104989","DOIUrl":"10.1016/j.bse.2025.104989","url":null,"abstract":"<div><div>The chemical study of <em>n</em>-hexane and ethyl acetate soluble fractions from the stem bark of <em>Parinari excelsa</em> led to the isolation of eleven secondary metabolites, including lupane (<strong>1</strong>, <strong>6</strong>, <strong>9</strong> and <strong>10</strong>), taraxarane (<strong>7</strong> and <strong>11</strong>), and fridelane (<strong>5</strong>) triterpenes, a steroidal glycoside (<strong>4</strong>) and three phenolic compounds (<strong>2</strong>, <strong>3</strong> and <strong>8</strong>). Their structures were established by spectroscopic techniques (especially 1D and 2D NMR) and high-resolution mass spectrometry, as well as by comparison with literature data. Apart from 3-<em>O</em>-β-<span>d</span>-glucopyranosyl-stigmasta-5,11(12)-diene (<strong>4</strong>), all these compounds are reported for the first time in the genus <em>Parinari</em>. Compounds <strong>3</strong>, <strong>4</strong> and <strong>10</strong> were found to be urease inhibitors with IC₅₀ values ranging from 10.8 to 22.4, while compounds <strong>1</strong> and <strong>5−8</strong> showed moderate activity with IC₅₀ ranging from 49.4 to 62.9 μM. The chemophenetic relevance of the isolates is discussed.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104989"},"PeriodicalIF":1.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the components and toxicity of the guttation of Alocasia cucullata (Lour.) Schott","authors":"Lue Hong ,&nbsp;Zerong Wu ,&nbsp;Jiahui Gong ,&nbsp;Jiahui Ma ,&nbsp;Shengzhi Liu","doi":"10.1016/j.bse.2025.104986","DOIUrl":"10.1016/j.bse.2025.104986","url":null,"abstract":"<div><div><em>Alocasia cucullata</em> (Lour.) Schott is a member of the Araceae family and is characterized by its unique leaf morphology and vibrant flowers; thus, it is a valuable addition to gardens and interior decor. However, <em>A. cucullata</em> may contain toxic compounds, including calcium oxalate crystals, lectins, and alkaloids, and their distribution within the plant tissues remains poorly understood. In this study, untargeted metabolomics was used to analyse the distribution of the bioactive compounds across the roots, stems, leaves, and guttation of <em>A. cucullata</em>. The toxic effects of these tissues on the MDA-MB-231 human breast cancer cell line were evaluated using the MTT assay. Microscopic techniques were utilized to examine the microstructure of the guttation and stems, and the focus was on the morphology of the calcium oxalate crystals. Liquid chromatography and ELISA kits were used to quantify the calcium oxalate and lectin contents in the various tissues. The results demonstrated that the active constituents, primarily fatty acids, phenylpropanoids, flavonoids, peptides, and alkaloids, were concentrated in the roots. Notably, guttation exhibited no toxicity and lacked both calcium oxalate crystals and significant lectin levels. This research enhances the understanding of the bioactive composition and safety profile of <em>A. cucullata</em> and provides a theoretical foundation for future scientific investigations and safe cultivation, with implications for both scientific and industrial applications.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104986"},"PeriodicalIF":1.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of chemical composition, nematicidal activity and acetylcholinesterase inhibition with in silico mechanistic insights of Hedychium flavescens essential oils and extracts from aerial parts and rhizomes","authors":"Himani Karakoti ,&nbsp;Pooja Bargali ,&nbsp;Ravendra Kumar ,&nbsp;Om Prakash ,&nbsp;Satya Kumar ,&nbsp;Stefania Garzoli ,&nbsp;Dharmendra Singh Rawat ,&nbsp;Jolanta Maslowiecka ,&nbsp;Valery A. Isidorov","doi":"10.1016/j.bse.2025.104987","DOIUrl":"10.1016/j.bse.2025.104987","url":null,"abstract":"<div><div><em>Hedychium flavescens</em> is recognized for its medicinal and pesticidal potential, yet its comprehensive phytochemical profile and bioactivity against nematodes remain underexplored. This study offers a comparative analysis of the chemical composition, nematicidal activity and acetylcholinesterase (AChE) inhibition of essential oils (EOs) and extracts from the plant's aerial and rhizome parts. GC-MS analysis identified significant chemical variation between the parts, with monoterpenes and sesquiterpenes predominating in both EOs. The major compounds were <em>α</em>-pinene, <em>β</em>-pinene, 1,8-cineole, linalool, and <em>E-</em>caryophyllene. On the other side, the extracts were dominated by fatty acids, sterols, and diterpenoids. Nematicidal activity was evaluated against <em>Meloidogyne incognita</em>, with rhizome EO showing higher potency (76% mortality at 1 μL/mL). Aerial and rhizome EOs exhibited significant nematode egg hatching inhibition, with 77.59–89.66% inhibition at 1000 μg/mL after 96 h of exposure. <em>In-vitro</em> AChE inhibition assays demonstrated potential neuroprotective properties in terms of IC₅₀ values with rhizome EO showing best inhibition activity (IC₅₀ = 181.03 μg/mL). Additionally, <em>in silico</em> docking studies provided insights into the mechanism of action of key compounds on AChE. The findings support <em>H. flavescens</em> as a natural alternative to synthetic nematicides and AChE inhibitors. Further studies are needed to explore its broader applications.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"121 ","pages":"Article 104987"},"PeriodicalIF":1.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical constituents from the fruits of Forsythia suspensa and their chemotaxonomic significance","authors":"Sui Liu ,&nbsp;Xiang-Xin Niu ,&nbsp;Jun-Lei He ,&nbsp;Chang-Hua Yue ,&nbsp;Zi-Xiao Jiang ,&nbsp;Zhang-Xin Yu ,&nbsp;Yang-Yang Liu","doi":"10.1016/j.bse.2025.104984","DOIUrl":"10.1016/j.bse.2025.104984","url":null,"abstract":"<div><div>A new labdane diterpenoid (4<em>R</em>,5<em>R</em>,7<em>R</em>,9<em>R</em>,10<em>R</em>)-methyl-7-hydroxylabda-8(17),13(<em>E</em>)-diene-18-carboxy-15-oate (<strong>1</strong>), along with three known analogues (<strong>2</strong>–<strong>4</strong>), a cyclohexenone derivative (<strong>5</strong>), three phenolic acids (<strong>6</strong>–<strong>8</strong>) and four lignan compounds (<strong>9</strong>–<strong>12</strong>), were isolated from the fruits of <em>Forsythia suspensa</em>. The structures were elucidated using comprehensive spectroscopic methods, including HRESIMS, 1D and 2D NMR, the absolute configuration of <strong>1</strong> was established based on quantum-chemical electronic circular dichroism (ECD) calculations. The compounds <strong>1</strong>–<strong>5</strong>, <strong>9</strong>, and <strong>11</strong> were evaluated for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced mouse monocyte macrophage (RAW 264.7) cells, as well as for their anti-cervical cancer activity. However, none of these compounds exhibited significant activity at a concentration of 50 μM. Notably, compounds <strong>1, 5</strong>, <strong>9,</strong> and <strong>11</strong> were reported here for the first time from the genus <em>Forsythia</em>, and this is also the first discovery of a cyclohexenone derivative within this genus. These findings contribute to the growing understanding of the chemical diversity and chemotaxonomic significance of <em>F. suspensa</em>.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"120 ","pages":"Article 104984"},"PeriodicalIF":1.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}