Biochemistry and Cell Biology最新文献_第7页

Distinct domain organization and diversity of 2'-5'-oligoadenylate synthetases. 2'-5'-oligoadenylate 合成酶不同的结构域和多样性。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-04-11 DOI: 10.1139/bcb-2023-0369

Amit Koul, Lok Tin Hui, Nikhat Lubna, Sean A McKenna

{"title":"Distinct domain organization and diversity of 2'-5'-oligoadenylate synthetases.","authors":"Amit Koul, Lok Tin Hui, Nikhat Lubna, Sean A McKenna","doi":"10.1139/bcb-2023-0369","DOIUrl":"10.1139/bcb-2023-0369","url":null,"abstract":"The 2'-5'-oligoadenylate synthetases (OAS) are important components of the innate immune system that recognize viral double-stranded RNA (dsRNA). Upon dsRNA binding, OAS generate 2'-5'-linked oligoadenylates (2-5A) that activate ribonuclease L (RNase L), halting viral replication. The OAS/RNase L pathway is thus an important antiviral pathway and viruses have devised strategies to circumvent OAS activation. OAS enzymes are divided into four classes according to size: small (OAS1), medium (OAS2), and large (OAS3) that consist of one, two, and three OAS domains, respectively, and the OAS-like protein (OASL) that consists of one OAS domain and tandem domains similar to ubiquitin. Early investigation of the OAS enzymes hinted at the recognition of dsRNA by OAS, but due to size differences amongst OAS family members combined with the lack of structural information on full-length OAS2 and OAS3, the regulation of OAS catalytic activity by dsRNA was not well understood. However, the recent biophysical studies of OAS have highlighted overall structure and domain organization. In this review, we present a detailed examination of the OAS literature and summarized the investigation on 2'-5'-oligoadenylate synthetases.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"305-318"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Antibacterial, anti-invasive, and anti-inflammatory activity of bovine lactoferrin extracted from milk or colostrum versus whole colostrum. 从牛奶或初乳中提取的牛乳铁蛋白与整个初乳相比，具有抗菌、抗侵入和抗炎活性。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI: 10.1139/bcb-2024-0021

Luigi Rosa, Giusi Ianiro, Antonietta Lucia Conte, Maria Pia Conte, Livia Ottolenghi, Piera Valenti, Antimo Cutone

{"title":"Antibacterial, anti-invasive, and anti-inflammatory activity of bovine lactoferrin extracted from milk or colostrum versus whole colostrum.","authors":"Luigi Rosa, Giusi Ianiro, Antonietta Lucia Conte, Maria Pia Conte, Livia Ottolenghi, Piera Valenti, Antimo Cutone","doi":"10.1139/bcb-2024-0021","DOIUrl":"10.1139/bcb-2024-0021","url":null,"abstract":"Lactoferrin (Lf), a multifunctional cationic glycoprotein extracted from milk or colostrum, is able to chelate two ferric ions per molecule, inhibit the formation of reactive oxygen species, interact with the anionic components of bacteria or host cells, and enter inside host cell nucleus, thereby exerting antibacterial, anti-invasive, and anti-inflammatory activities. By virtue of Lf presence, bovine colostrum is expected to perform analogous functions to pure Lf, along with additional activities attributable to other bioactive constituents. The present research aims to compare the antibacterial, anti-invasive, and anti-inflammatory activities of bovine Lf purified from milk (mbLf) and colostrum (cbLf) in comparison to those exhibited by whole bovine colostrum (wbc). The results demonstrated a major efficacy of mbLf in inhibiting pathogenic bacteria and in exerting anti-invasive and anti-survival activities with respect to cbLf and wbc. Furthermore, mbLf lowered IL-6 levels to those of uninfected cells, while a less evident decrease was observed upon cbLf treatment. Conversely, wbc managed to slightly lower IL-6 levels compared to those synthesized by infected cells. These data demonstrate that, to obtain maximum effectiveness in such activities, Lf should be formulated/used without addition of other substances and should be sourced from bovine milk rather than colostrum.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"331-341"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of cells expressing lipocalin-2 (LCN2) as a reporter. 表达脂联素-2（LCN2）作为报告基因的细胞的特征。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-05-02 DOI: 10.1139/bcb-2024-0013

Kimihiko Sugaya

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The journey from bench to bedside-it takes a science village. 从工作台到床边--这需要一个科学村。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-04-19 DOI: 10.1139/bcb-2024-0075

Anne-Marie Mes-Masson

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SENP1 knockdown potentiates the apoptosis, cell cycle arrest, and reduces cisplatin resistance of diffuse large B cell lymphoma cells via inducing ferroptosis. SENP1 基因敲除可通过诱导铁蛋白沉积促进弥漫大 B 细胞淋巴瘤细胞凋亡、细胞周期停滞并降低顺铂抗性。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI: 10.1139/bcb-2023-0285

Jinfeng Dong, Xiaoqiang Zheng

{"title":"SENP1 knockdown potentiates the apoptosis, cell cycle arrest, and reduces cisplatin resistance of diffuse large B cell lymphoma cells via inducing ferroptosis.","authors":"Jinfeng Dong, Xiaoqiang Zheng","doi":"10.1139/bcb-2023-0285","DOIUrl":"10.1139/bcb-2023-0285","url":null,"abstract":"Ferroptosis has been regarded as a critical event in the process of diffuse large B cell lymphoma (DLBCL). Sentrin-specific protease 1 (SENP1) has emerged as an oncogene in multiple human malignancies. The present work was to investigate the effects of SENP1 on the progression of DLBCL and the possible regulatory mechanism involving ferroptosis. SENP1 expression in DLBCL tissues, parental and cisplatin-resistant DLBCL cells were, respectively, tested by GEPIA database, RT-qPCR, and Western blot. Cell viability was estimated via CCK-8 assay. Flow cytometry analysis estimated cell apoptosis and cycle. Western blot examined the expression of apoptosis-, cell cycle-, and ferroptosis-associated proteins. TBARS assay and BODIPY 581/591 C11 probe measured lipid peroxidation. Related assay kit assessed total iron levels. CCK-8 and flow cytometry evaluated cisplatin resistance. SENP1 expression was raised in DLBCL tissues and cells. SENP1 knockdown reduced cell viability, boosted cell apoptosis, cell cycle arrest, and elevated cisplatin sensitivity in DLBCL. SENP1 depletion drove the ferroptosis of both parental and cisplatin-resistant DLBCL cells and ferroptosis inhibitor Fer-1 reversed the influences of SENP1 inhibition on cell viability, apoptosis, cell cycle, and cisplatin resistance in DLBCL. Anyway, SENP1 absence might facilitate ferroptosis to obstruct the development of DLBCL and cisplatin resistance.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"319-330"},"PeriodicalIF":2.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-based design of small molecule inhibitors of the cagT4SS ATPase Cagα of Helicobacter pylori. 基于结构设计的幽门螺旋杆菌 cagT4SS ATP 酶 Cagα 小分子抑制剂。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-02-20 DOI: 10.1139/bcb-2023-0331

Claire Morin, Vijay Tailor Verma, Tarun Arya, Bastien Casu, Eric Jolicoeur, Réjean Ruel, Anne Marinier, Jurgen Sygusch, Christian Baron

{"title":"Structure-based design of small molecule inhibitors of the cagT4SS ATPase Cagα of Helicobacter pylori.","authors":"Claire Morin, Vijay Tailor Verma, Tarun Arya, Bastien Casu, Eric Jolicoeur, Réjean Ruel, Anne Marinier, Jurgen Sygusch, Christian Baron","doi":"10.1139/bcb-2023-0331","DOIUrl":"10.1139/bcb-2023-0331","url":null,"abstract":"We here describe the structure-based design of small molecule inhibitors of the type IV secretion system of Helicobacter pylori. The secretion system is encoded by the cag pathogenicity island, and we chose Cagα, a hexameric ATPase and member of the family of VirB11-like proteins, as target for inhibitor design. We first solved the crystal structure of Cagα in a complex with the previously identified small molecule inhibitor 1G2. The molecule binds at the interface between two Cagα subunits and mutagenesis of the binding site identified Cagα residues F39 and R73 as critical for 1G2 binding. Based on the inhibitor binding site we synthesized 98 small molecule derivates of 1G2 to improve binding of the inhibitor. We used the production of interleukin-8 of gastric cancer cells during H. pylori infection to screen the potency of inhibitors and we identified five molecules (1G2_1313, 1G2_1338, 1G2_2886, 1G2_2889, and 1G2_2902) that have similar or higher potency than 1G2. Differential scanning fluorimetry suggested that these five molecules bind Cagα, and enzyme assays demonstrated that some are more potent ATPase inhibitors than 1G2. Finally, scanning electron microscopy revealed that 1G2 and its derivatives inhibit the assembly of T4SS-determined extracellular pili suggesting a mechanism for their anti-virulence effect.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"226-237"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The sperm nuclear basic proteins of the sword fern (Polystichum munitum). 剑蕨（Polystichum munitum）的精子核基本蛋白。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-02-12 DOI: 10.1139/bcb-2023-0343

Juan Ausió, Alistair Knox, Bo-Hyun Kim, Elaine Humphrey, Brent Gowen, Naoki Minamino, Patrick von Aderkas

引用次数: 0

Cell-in-cell-mediated intercellular communication exacerbates the pro-inflammatory progression in asthma. 细胞间介导的细胞间通讯加剧了哮喘的炎症进展。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-04-03 DOI: 10.1139/bcb-2023-0228

Shan Wang, Bowen Liu, Huiru He, Jiahao Huang, Fangping He, Ying He, Ailin Tao

{"title":"Cell-in-cell-mediated intercellular communication exacerbates the pro-inflammatory progression in asthma.","authors":"Shan Wang, Bowen Liu, Huiru He, Jiahao Huang, Fangping He, Ying He, Ailin Tao","doi":"10.1139/bcb-2023-0228","DOIUrl":"10.1139/bcb-2023-0228","url":null,"abstract":"Cell-in-cell (CIC) structures have been suggested to mediate intracellular substance transport between cells and have been found widely in inflammatory lung tissue of asthma. The aim of this study was to investigate the significance of CIC structures in inflammatory progress of asthma. CIC structures and related inflammatory pathways were analyzed in asthmatic lung tissue and normal lung tissue of mouse model. In vitro, the activation of inflammatory pathways by CIC-mediated intercellular communication was analyzed by RNA-Seq and verified by Western blotting and immunofluorescence. Results showed that CIC structures of lymphocytes and alveolar epithelial cells in asthmatic lung tissue mediated intercellular substance (such as mitochondria) transfer and promoted pro-inflammation in two phases. At early phase, internal lymphocytes triggered inflammasome-dependent pro-inflammation and cell death of itself. Then, degraded lymphocytes released cellular contents such as mitochondria inside alveolar epithelial cells, further activated multi-pattern-recognition receptors and NF-kappa B signaling pathways of alveolar epithelial cells, and thereby amplified pro-inflammatory response in asthma. Our work supplements the mechanism of asthma pro-inflammation progression from the perspective of CIC structure of lymphocytes and alveolar epithelial cells, and provides a new idea for anti-inflammatory therapy of asthma.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"262-274"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Granulocyte pro-myeloperoxidase is redundantly processed by proprotein convertase furin and PC7 in HL-60 cells. 粒细胞前髓过氧化物酶在 HL-60 细胞中被前蛋白转化酶呋喃和 PC7 重复处理。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-03-14 DOI: 10.1139/bcb-2023-0339

Vanessa Lapointe, Frédéric Couture

{"title":"Granulocyte pro-myeloperoxidase is redundantly processed by proprotein convertase furin and PC7 in HL-60 cells.","authors":"Vanessa Lapointe, Frédéric Couture","doi":"10.1139/bcb-2023-0339","DOIUrl":"10.1139/bcb-2023-0339","url":null,"abstract":"Neutrophil myeloperoxidase/H2O2/chloride system is a key mechanism to control pathogen infection. This enzyme, myeloperoxidase, plays a pivotal role in the arsenal of azurophilic granules that are released through degranulation upon neutrophil activation, which trigger local hypochlorous acid production. Myeloperoxidase gene encodes a protein precursor named promyeloperoxidase that arbors a propeptide that gets cleaved later during secretory routing in post-endoplasmic reticulum compartments. Although evidence suggested that this processing event was performed by one or different enzymes from the proprotein convertases family, the identity of this enzyme was never investigated. In this work, the naturally producing myeloperoxidase promyelocytic cell line HL-60 was used to investigate promyeloperoxidase cleavage during granulocytic differentiation in response to proprotein convertase inhibitors decanoyl-RVKR-chloromethylketone and hexa-d-arginine. Stable PC knockdown of endogenously expressed proprotein convertases, furin and PC7, was achieved using lentiviral delivery of shRNAs. None of the knockdown cell line could reproduce the effect of the pan-proprotein convertases inhibitor decanoyl-RVKR-chloromethylketone that accumulated intracellular promyeloperoxidase stores in HL-60 cells, therefore illustrating that both furin and PC7 redundantly process this proprotein.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"275-284"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140130651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protamines and the sperm nuclear basic proteins Pandora's Box of insects. 原胺和精子核基本蛋白昆虫的潘多拉魔盒

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-02-26 DOI: 10.1139/bcb-2023-0363

Melissa R Leyden, Brent Gowen, Rodrigo Gonzalez-Romero, Jose Maria Eirin-Lopez, Bo-Hyun Kim, Fumio Hayashi, Jay McCartney, Patrick C Zhang, Miyoko Kubo-Irie, Jeffrey Shabanowitz, Donald F Hunt, Patrick Ferree, Harold Kasinsky, Juan Ausió

{"title":"Protamines and the sperm nuclear basic proteins Pandora's Box of insects.","authors":"Melissa R Leyden, Brent Gowen, Rodrigo Gonzalez-Romero, Jose Maria Eirin-Lopez, Bo-Hyun Kim, Fumio Hayashi, Jay McCartney, Patrick C Zhang, Miyoko Kubo-Irie, Jeffrey Shabanowitz, Donald F Hunt, Patrick Ferree, Harold Kasinsky, Juan Ausió","doi":"10.1139/bcb-2023-0363","DOIUrl":"10.1139/bcb-2023-0363","url":null,"abstract":"Insects are the largest group of animals when it comes to the number and diversity of species. Yet, with the exception of Drosophila, no information is currently available on the primary structure of their sperm nuclear basic proteins (SNBPs). This paper represents the first attempt in this regard and provides information about six species of Neoptera: Poecillimon thessalicus, Graptosaltria nigrofuscata, Apis mellifera, Nasonia vitripennis, Parachauliodes continentalis, and Tribolium castaneum. The SNBPs of these species were characterized by acetic acid urea gel electrophoresis (AU-PAGE) and high-performance liquid chromatography fractionated. Protein sequencing was obtained using a combination of mass spectrometry sequencing, Edman N-terminal degradation sequencing and genome mining. While the SNBPs of several of these species exhibit a canonical arginine-rich protamine nature, a few of them exhibit a protamine-like composition. They appear to be the products of extensive cleavage processing from a precursor protein which are sometimes further processed by other post-translational modifications that are likely involved in the chromatin transitions observed during spermiogenesis in these organisms.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"238-251"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0