Biochemistry and Cell Biology最新文献_第7页

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0284

引用次数: 0

Treatment of lactoferrin and antimicrobial peptide N6 on bacterial enteritis caused by Escherichia coli in mice. 乳铁蛋白和抗菌肽 N6 对大肠杆菌引起的小鼠细菌性肠炎的治疗作用。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2024-10-11 DOI: 10.1139/bcb-2024-0103

Xuanxuan Ma, Kun Zhang, Na Yang, Ya Hao, Ruoyu Mao, Da Teng, Jianhua Wang

{"title":"Treatment of lactoferrin and antimicrobial peptide N6 on bacterial enteritis caused by Escherichia coli in mice.","authors":"Xuanxuan Ma, Kun Zhang, Na Yang, Ya Hao, Ruoyu Mao, Da Teng, Jianhua Wang","doi":"10.1139/bcb-2024-0103","DOIUrl":"10.1139/bcb-2024-0103","url":null,"abstract":"Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in humans and animals. The study aimed to evaluate the efficacy of bovine lactoferrin (bLf) as an adjuvant combined with AMP (N6) in the treatment of E. coli-induced bacterial enteritis. Firstly, 40 female ICR mice were randomly divided into four groups. The ETEC-A, ETEC-B, and ETEC-C groups were gavaged with 0.2 mL of ETEC K88 at 5 × 109, 5 × 108, and 5 × 107 CFU/mL for three consecutive days, respectively, the CK control group was given PBS. Based on the clinical symptoms and intestinal changes, the optimal model dose of ETEC K88 was determined to be 5 × 108 CFU/mL. Sixty female ICR mice were randomly divided into six groups: CK group (uninfected), NC group (infected and untreated), N6 treatment group (20 mg/kg), bLf treatment group (100 mg/kg), bLf + N6-A treatment group (10 mg/kg N6+100 mg/kg bLf), and bLf + N6-B group (20 mg/kg N6+100 mg/kg bLf). The clinical symptoms, intestinal morphology, inflammatory response and serum metabolites were monitored. The results showed that compared with the NC group, the bLf-N6-A and bLf-N6-B treatment groups had significant reductions in TNF-α and IL-6, significant increases in IL-10, and significant reductions in endotoxin and DAO in plasma (p < 0.05). Meanwhile, the bLf-N6-A and bLf-N6-B treatment groups significantly increased the expression of ZO-1, claudin-1 and occludin, increased the height of small intestinal mucosal villi and VH/CD after ETEC K88-induced intestinal injury (p < 0.05). The combination of bLf and N6 relieved enteritis by balancing intestinal mucosal immunity, improving intestinal morphology and barrier function. BLf combined with N6 can be used as an effective therapeutic strategy for the treatment of bacterial enteritis.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrocortisone improves post-resuscitation myocardial dysfunction by inhibiting the NF-κB pathway. 氢化可的松通过抑制 NF-κB 通路改善复苏后心肌功能障碍。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2024-11-13 DOI: 10.1139/bcb-2024-0162

Yaqin Fang, Fenglin Song, Chunyan Gao, Zhiming Wang

{"title":"Hydrocortisone improves post-resuscitation myocardial dysfunction by inhibiting the NF-κB pathway.","authors":"Yaqin Fang, Fenglin Song, Chunyan Gao, Zhiming Wang","doi":"10.1139/bcb-2024-0162","DOIUrl":"10.1139/bcb-2024-0162","url":null,"abstract":"Myocardial dysfunction is a major cause of early mortality after successful cardiopulmonary resuscitation (CPR) following cardiac arrest (CA). Following the return of spontaneous circulation, myocardial ischemia-reperfusion injury can activate the NF-κB pathway, leading to the transcription of inflammatory genes that impair myocardial function. While clinical studies show hydrocortisone (HC) improves outcomes in CA patients during CPR, its specific role in modulating the NF-κB pathway is unclear. In this study, we established an in vitro model by inducing hypoxia/reoxygenation (H/R) injury in H9C2 cardiomyocytes using Na2S2O4, followed by HC treatment. The results showed that HC treatment of H/R-injured cardiomyocytes promoted proliferation, inhibited apoptosis, and suppressed the NF-κB pathway, thereby reducing interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-alpha (TNF-α) levels. Moreover, inhibition of the NF-κB pathway enhanced the proliferative capacity of H/R cardiomyocytes, decreased apoptosis rates, and reduced IL-6, IL-8, and TNF-α expression levels, with these effects being further amplified by HC treatment. These findings were further supported by in vivo experiments. In conclusion, our study suggests that HC may promote H/R cardiomyocyte proliferation, inhibit apoptosis, and alleviate inflammatory responses by suppressing the NF-κB pathway, providing new evidence to support its potential clinical application in CA management.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell RNA sequencing analysis reveals the heterogeneity and effect of TAMs in colorectal cancer. 单细胞RNA测序分析揭示了tam在结直肠癌中的异质性和作用。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0041

Chengang Wang, Ying Qian, Mingsheng Zhang

{"title":"Single-cell RNA sequencing analysis reveals the heterogeneity and effect of TAMs in colorectal cancer.","authors":"Chengang Wang, Ying Qian, Mingsheng Zhang","doi":"10.1139/bcb-2025-0041","DOIUrl":"10.1139/bcb-2025-0041","url":null,"abstract":"Colorectal cancer (CRC) is a prevalent and malignant tumor of the digestive system, characterized by high incidence and mortality rates. This study aimed to investigate the heterogeneity of the tumor microenvironment (TME) and the involvement of immune cells in CRC. Single-cell RNA sequencing (scRNA-seq) data obtained from the Gene Expression Omnibus database were used to analyze and identify six major cell types across normal, core, and border tumor samples. A total of 27 414 cells from various regions of patients with CRC were selected for subsequent analyses. Cellular interaction analysis revealed that differential signaling pathways between the TME and normal tissues, with several pathways involving interactions between myeloid cells and epithelial cells. Myeloid cells were extracted and classified into six subtypes based on markers identified in the literature. Monocle3 revealed the trajectory of tumor-associated macrophages (TAMs) and identified genes associated with pseudotime. Single-Cell ENrichment analysis for Interpreting Cellular Heterogeneity analysis identified specific regulons and target genes associated with TAMs. This study reanalyzed single-cell RNA-sequencing data and provided insights into the heterogeneity of the TME, particularly in relation to the role of TAMs.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LMAN2 interacts with HEATR3 to expedite HER2-positive breast cancer advancement and inflammation and Akt/ERK/NF-κB signaling. LMAN2与heat3相互作用，加速her2阳性乳腺癌进展和炎症以及Akt/ERK/NF-κB信号传导。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2025-01-08 DOI: 10.1139/bcb-2024-0166

Sujian Xiao, Tong Yu, Fulan Yang, Huozhong Yuan, Jun Ni

{"title":"LMAN2 interacts with HEATR3 to expedite HER2-positive breast cancer advancement and inflammation and Akt/ERK/NF-κB signaling.","authors":"Sujian Xiao, Tong Yu, Fulan Yang, Huozhong Yuan, Jun Ni","doi":"10.1139/bcb-2024-0166","DOIUrl":"10.1139/bcb-2024-0166","url":null,"abstract":"The paper aimed to reveal the impacts and the possible mechanism of action of lectin mannose-binding 2 protein (LMAN2) in HER2-positive breast cancer (BC). The expression, prognostic potential of LMAN2, and the correlation between LMAN2 and HEAT repeat containing 3 (HEATR3) in BC were analyzed in TCGA database. Intact, Mentha, and BioGrid databases predicted LMAN2-HEATR3 interactions. Reverse transcription-quantitative PCR and Western blot examined LMAN2 expression. Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine staining, wound healing, and transwell assays, respectively, detected the aggressive cellular biological behaviors including proliferation, migration, and invasion. Western blot analyzed the expression of matrix metalloproteinases, HEATR3, and protein kinase B (Akt)/extracellular signal-regulated kinase (ERK)/nuclear factor-kappaB (NF-κB) signaling-related proteins. Co-immunoprecipitation assay was used to prove the relationship of LMAN2 with HEATR3. Enzyme-linked immunosorbent assay detected inflammatory cytokine levels. LMAN2 was overexpressed in HER2-positive BC tissues and cells and indicated unfavorable prognosis of BC patients. LMAN2 knockdown suppressed HER2-positive BC cell proliferation, migration, and invasion. LMAN2 interacted with and had a positive correlation with HEATR3. HEATR3 up-regulation reversed the repressive role of LMAN2 interference in the progression of HER2-positive BC, Akt/ERK/NF-κB signaling, and inflammatory response. Altogether, LMAN2 silencing might exert anti-tumor and anti-inflammatory properties and inactivate Akt/ERK/NF-κB signaling in HER2-positive BC via binding to HEATR3.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitotic bookmarking provides epigenetic persistence or plasticity for biological control and cancer. 有丝分裂书签为生物控制和癌症提供表观遗传持久性或可塑性。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0047

Andrew J Fritz, Emory Pacht, Rabail H Toor, Janine S Warren, Haley W Greenyer, Jackson R Del Porto, Abigail G Person, Sadie J Korzec, Georgiy Zotkin, Jessica L Heath, Prachi N Ghule, Jonathan A R Gordon, Andre J Van Wijnen, Seth E Frietze, Karen C Glass, Jane B Lian, Janet L Stein, Gary S Stein

{"title":"Mitotic bookmarking provides epigenetic persistence or plasticity for biological control and cancer.","authors":"Andrew J Fritz, Emory Pacht, Rabail H Toor, Janine S Warren, Haley W Greenyer, Jackson R Del Porto, Abigail G Person, Sadie J Korzec, Georgiy Zotkin, Jessica L Heath, Prachi N Ghule, Jonathan A R Gordon, Andre J Van Wijnen, Seth E Frietze, Karen C Glass, Jane B Lian, Janet L Stein, Gary S Stein","doi":"10.1139/bcb-2025-0047","DOIUrl":"10.1139/bcb-2025-0047","url":null,"abstract":"Mitotic bookmarking, the retention of regulatory proteins and lncRNAs on chromatin during mitosis, epigenetically sustains competency for phenotype-specific gene expression in progeny cells. Gene expression is predominantly suppressed during mitosis. Bookmarking provides the guidance for the resumption of gene expression in progeny cells that is obligatory for physiological control of lineage commitment, specialized cell structure and phenotypic function. While regulatory continuity is supported by the persistence of genome-associated regulatory complexes, altered bookmarking mediates plasticity for responsiveness to physiological cues. Bookmarking fidelity ensures genome integrity and controls expression of tumor suppressors and proto-oncogenes. Cancer-compromised aberrations in bookmarking results in transcriptional dysregulation and the initiation of tumor-associated processes.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-10"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: LncRNA MALAT1 regulates diabetic cardiac fibroblasts through the Hippo-YAP signaling pathway. 撤稿：LncRNA MALAT1通过Hippo-YAP信号通路调控糖尿病心脏成纤维细胞。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0021

引用次数: 0

Effects of different sources of lactoferrin on cytokine response to SARS-COV-2, respiratory syncytial virus, and rotavirus infection in vitro. 不同来源的乳铁蛋白对体外 SARS-COV-2、呼吸道合胞病毒和轮状病毒感染的细胞因子反应的影响。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0146

Rulan Jiang, Xiaogu Du, Bo Lönnerdal

{"title":"Effects of different sources of lactoferrin on cytokine response to SARS-COV-2, respiratory syncytial virus, and rotavirus infection in vitro.","authors":"Rulan Jiang, Xiaogu Du, Bo Lönnerdal","doi":"10.1139/bcb-2024-0146","DOIUrl":"10.1139/bcb-2024-0146","url":null,"abstract":"Lactoferrin (Lf) is a multifunctional iron-binding glycoprotein, involved in a wide range of bioactivities, including immunomodulatory and antiviral activities. Lf in human milk and bovine Lf added to infant formula may provide some protection against viral infections. However, functions of Lfs from different sources may differ due to varying manufacturing processes and posttranslational modifications. Here, effects of Lfs (11 commercial bovine milk Lfs, 2 recombinant Lfs, and native human/bovine milk Lf) on cytokine responses to virus infection were examined by infecting human intestinal epithelial cells (Caco-2 cells) with rotavirus (naked) or normal human bronchial epithelial cells (BEAS-2B cells) with respiratory syncytial virus (RSV, enveloped) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein 1. Effects of Lf on viral infection were evaluated by quantitative real-time polymerase chain reaction analysis of transcripts of cytokines/chemokines (TNF-α, IL-1β, IL-6, IL-8, IL-10, IFN-β, and CXCL10). Our results show that viral infection changes transcription of these cytokines and that Lfs significantly and variously influence immune responses to rotavirus, RSV, and SARS-CoV-2 in vitro. Thus, Lf may provide protection against virus infection by down-regulating pro-inflammatory cytokine/chemokine responses. Recombinant bovine and human Lf show similar effects as bovine milk Lfs suggesting that different posttranslational modifications do not affect the antiviral activity on cytokine response.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting galectin-1, -3, -4, and -9 as biotargets for colorectal cancer. 重述半乳糖凝集素-1、-3、-4和-9作为结直肠癌的生物靶点。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0218

Ammar Akram Kamarudin, Nadiah Abu

引用次数: 0

Lactoferrin enhances the antibiotic treatment of Staphylococcus aureus in bone infection. 乳铁蛋白增强了金黄色葡萄球菌骨感染的抗生素治疗。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0101

Jillian Cornish, Reece Joseph, Jian-Ming Lin, Stuart G Irwin, Janesha Perera, Karen E Callon, Jagir R Hassan, Jingyuan Wen, Haemish Crawford, Brya G Matthews, Nicholas N Ashton, D Williams, Heather M Baker, Eduard N Baker, Simon Swift

{"title":"Lactoferrin enhances the antibiotic treatment of Staphylococcus aureus in bone infection.","authors":"Jillian Cornish, Reece Joseph, Jian-Ming Lin, Stuart G Irwin, Janesha Perera, Karen E Callon, Jagir R Hassan, Jingyuan Wen, Haemish Crawford, Brya G Matthews, Nicholas N Ashton, D Williams, Heather M Baker, Eduard N Baker, Simon Swift","doi":"10.1139/bcb-2024-0101","DOIUrl":"https://doi.org/10.1139/bcb-2024-0101","url":null,"abstract":"Lactoferrin (Lf), we have previously shown, has therapeutic potential in the field of skeletal regenerative medicine demonstrating its potent stimulating effects on bone growth. Recently, we have identified bovine lactoferrin (bLf) as a factor that also enhances antibiotic killing of Staphylococcus aureus (S. aureus). Biofilms are associated with around 65% of all infections and 80% of chronic infections. One feature of biofilm infection is tolerance to antibiotics due to the survival of a subpopulation of biofilm bacteria, where laboratory tests on planktonic cells indicate susceptibility. Tolerance is seen in bone infections of osteomyelitis and prosthetic joints, where methicillin-susceptible S. aureus (MSSA) strains predominate, but where treatments with the frontline penicillinase-resistant antibiotic cefazolin (CEF) can be ineffective. In vitro-grown biofilms of MSSA are 1000-fold more tolerant to CEF but can be eradicated by CEF at 10x minimal inhibitory concentration in the presence of bLf. Bone infection can impede blood circulation within the bone, leading to bone death. Lf as a potent stimulator of bone growth adds to its appeal as a treatment for bone infections.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":"103 ","pages":"1-5"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0