Biochemistry and Cell Biology最新文献_第6页

Equity in action: a 4-year journey towards gender parity and racial diversity in biochemistry hiring. 行动中的公平：生物化学招聘中性别平等和种族多样性的四年之旅。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0114

Sherri L Christian, Valerie Booth, Scott V Harding, Amy M Todd, Mark D Berry

{"title":"Equity in action: a 4-year journey towards gender parity and racial diversity in biochemistry hiring.","authors":"Sherri L Christian, Valerie Booth, Scott V Harding, Amy M Todd, Mark D Berry","doi":"10.1139/bcb-2025-0114","DOIUrl":"10.1139/bcb-2025-0114","url":null,"abstract":"Recruitment of faculty members in academic departments shapes the department for decades in research and teaching arenas. A diverse department is beneficial for all students as representation of underrepresented minority groups in the professoriate can inspire a greater diversity of students to pursue higher levels of education or research-focused careers. Increased diversity benefits research directly as diverse teams have been shown to have better ideas and outcomes. In 2020, our department had lower gender diversity than expected based on the pool of qualified personnel in Canada. Therefore, we altered our hiring process, primarily by redacting applications, for recruitment into entry-level tenure-track faculty positions. This resulted in the increased hiring of women (17% to 80%) with no substantial change in hiring of racially diverse individuals (50% to 40%). Overall, combined with retirements, the percentage of women faculty in the department went from 25% to 50% and the percentage of racialized faculty went from 38% to 44%. Thus, our intervention was successful in increasing the diversity of our department within a short timeframe. Our experience could provide other departments with a template for making substantive change, even in the absence of internal expertise in the area.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-GlcNAcylation of epidermal growth factor receptor and glucose transporter 1 prevents their intrinsic down regulation in breast cancer cells. 在乳腺癌细胞中，表皮生长因子受体和葡萄糖转运蛋白1的o - glcn酰化阻止了它们的内在下调。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0055

G Pauline Padilla-Meier, Yeshika Bhatia, Suresh Mishra

{"title":"O-GlcNAcylation of epidermal growth factor receptor and glucose transporter 1 prevents their intrinsic down regulation in breast cancer cells.","authors":"G Pauline Padilla-Meier, Yeshika Bhatia, Suresh Mishra","doi":"10.1139/bcb-2025-0055","DOIUrl":"10.1139/bcb-2025-0055","url":null,"abstract":"The hexosamine biosynthetic pathway (HBP) is upregulated in many cancer cell types leading to upregulation of post-translational modification of proteins by β-N-acetylglucosamine (O-GlcNAc), the product of HBP. However, our knowledge of the identity of proteins that undergo O-GlcNAcylation in cancer cells and consequently their roles is very limited. We investigated the O-GlcNAcylation of epidermal growth factor receptor (EGFR) and glucose transporter 1 (GLUT1) in T47D and MDA-MB-231 breast cancer cell models. We examined the effect of the loss of putative O-GlcNAcylation sites in EGFR and GLUT1 on cell-signaling pathways and their functional consequences on cell cycle progression and cell metabolism using fluorescence-activated cell sorting analysis and in vitro assays. EGFR and GLUT1 undergo O-GlcNAcylation in T47D and MDA-MB-231 breast cancer cells, which enhances their functions and prevents their intrinsic downregulation. This appears to involve an interplay between phosphorylation, O-GlcNAcylation, and ubiquitination in both proteins. Importantly, perturbing the putative O-GlcNAcylation sites in both proteins adversely affected their stability, functions, and metabolic status of breast cancer cells, including glucose uptake and lactate production. In conclusion, the reprogrammed metabolism in cancer cells extends beyond energy and macromolecule requirements and contributes to cell-signaling events that support the stability and function of cancer promoting proteins.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-13"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactoferrin in the Eternal City. 永恒之城的乳铁蛋白。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0217

Piera Valenti, Antimo Cutone, Luigi Rosa, Hans J Vogel

引用次数: 0

A combinatorial multi-site directed mutagenesis solution for improved thermal stability of Lactobacillus plantarum tannase. 提高植物乳杆菌单宁酶热稳定性的组合多位点定向诱变溶液。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0134

T Deandre Chevannes, Antony D St-Jacques, Matthew E Loewen, Michele C Loewen

{"title":"A combinatorial multi-site directed mutagenesis solution for improved thermal stability of Lactobacillus plantarum tannase.","authors":"T Deandre Chevannes, Antony D St-Jacques, Matthew E Loewen, Michele C Loewen","doi":"10.1139/bcb-2025-0134","DOIUrl":"10.1139/bcb-2025-0134","url":null,"abstract":"This study used a modified flapless (FLT) version of tannase from Lactobacillus plantarum, (LpTan) to explore the effects of \"stacking\" site mutations predicted by Protein Repair One Stop Shop (PROSS) to increase stability. Four different LpTan structural-state models (including apo, substrate- and product- bound as well as FLT) were comparatively applied, yielding 143 predicted mutations. Of these, eight mutations (including Q63T, A65D, A184Y, A257D, V276Y, T321G, G421D, and G439D (FLT numbering)) were selected to stack, based on conservation of the prediction across all four structural states. Combinatorial screening of the arising 256-member library yielded a selection of possible hits, of which four were further characterized. Variant P6H7 contained 7 of the 8 mutations (excluding V276Y) and showed the highest significant kcat, 17% higher than FLT and 30% higher than LpTan, and a 4.5 °C increase in Tm . Variant P8E5 with 6 of 8 mutations (excluding A257D and G439D), yielded a 6.5 °C increase in Tm compared to FLT. The two other variants showed more moderate increases, albeit still greater than FLT or LpTan. Overall, the ability to design thermal stabilized versions of a tannase is emphasized. Putative mechanisms underlying the stabilization imparted by the highlighted variations are discussed.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational screening of filamin mechanical binding proteins using AlphaFold2. 利用AlphaFold2计算筛选丝蛋白机械结合蛋白。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0194

Jennifer Johnson, Nicanor González-Morales

{"title":"Computational screening of filamin mechanical binding proteins using AlphaFold2.","authors":"Jennifer Johnson, Nicanor González-Morales","doi":"10.1139/bcb-2025-0194","DOIUrl":"10.1139/bcb-2025-0194","url":null,"abstract":"Filamins are dimeric actin-binding protein that play a critical role in mechanical signaling. They contain a mechanosensory region (MSR) that naturally folds into a globular closed conformation. Under mechanical stress, the MSR unfolds into an open conformation, exposing binding sites for numerous proteins. Filamins are involved in diverse cellular functions, and their mechanical binding targets are highly context-dependent. In this study, we employed AlphaFold2 modelling for screening proteins that specifically recognize the open conformation of filamins. We focused on the Drosophila melanogaster filamin, Cheerio, and conducted a biased screen to identify mechanical binding proteins. We selected the top 132 hits from the initial screening for further characterization. All identified binding proteins specifically recognize the open conformation of the MSR and not the closed conformation. Interestingly, the binding regions of these proteins lack obvious sequence similarity. While some false positives were identified, they could be effectively filtered out based on the secondary structure formed at the binding interface. This study provides a framework for identifying specifically filamin interactions in mechanosignaling.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiofilm activities of lactoferricin-related Trp- and Arg-rich antimicrobial hexapeptides against pathogenic Staphylococcus aureus and Pseudomonas aeruginosa strains. 乳铁蛋白相关的富含 Trp 和 Arg 的抗菌六肽对致病性金黄色葡萄球菌和铜绿假单胞菌菌株的抗生物膜活性。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2024-10-17 DOI: 10.1139/bcb-2024-0183

Gopal Ramamourthy, Hans J Vogel

{"title":"Antibiofilm activities of lactoferricin-related Trp- and Arg-rich antimicrobial hexapeptides against pathogenic Staphylococcus aureus and Pseudomonas aeruginosa strains.","authors":"Gopal Ramamourthy, Hans J Vogel","doi":"10.1139/bcb-2024-0183","DOIUrl":"10.1139/bcb-2024-0183","url":null,"abstract":"Recently, several antimicrobial peptides (AMPs), varying in length from 12 to 37 residues, have been shown to act as antibiofilm agents. Here, we report a study of 23 hexapeptides modeled after four different Trp- and Arg-rich AMPs, including the RRWQWR-NH2 peptide, derived from bovine lactoferrin. They were tested against the pathogenic Gram-negative Pseudomonas aeruginosa PAO1 strain and a Gram-positive Staphylococcus aureus MRSA strain. Both strains were engineered to express the green fluorescent protein (GFP) protein, and fluorescence detection was used to measure the ability of the peptides to prevent biofilm formation (minimum biofilm inhibitory concentration (MBIC)) or to cause the breakdown of established biofilms (minimum biofilm eradication concentration (MBEC)). Similar antibiofilm activities were obtained with the standard crystal violet dye assay. Most Trp- and Arg-rich hexapeptides displayed a potent antibiofilm activity against the Gram-positive S. aureus MRSA strain. In particular, hexapeptides with 3 Arg and 3 Trp were very effective, especially when they contained the three Trp in sequence. Somewhat unexpectedly, the antimicrobial (MIC) values correlated with the MBIC and MBEC values, which has not been seen for several other AMP/antibiofilm peptides. Our results demonstrate that short Trp- and Arg-rich peptides merit further studies as antibiofilm agents that could be deployed to address part of the antimicrobial resistance problem.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-18"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and meta-analysis. 乳铁蛋白补充对儿童感染的疗效：一项系统综述和荟萃分析。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0181

Valerie S Mayorga, Rafaella Navarro, Victor D Torres Roldan, Meritxell Urtecho, Silvia Tipe, Bea Calvert, Laura A Wright, Theresa J Ochoa

{"title":"Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and meta-analysis.","authors":"Valerie S Mayorga, Rafaella Navarro, Victor D Torres Roldan, Meritxell Urtecho, Silvia Tipe, Bea Calvert, Laura A Wright, Theresa J Ochoa","doi":"10.1139/bcb-2024-0181","DOIUrl":"10.1139/bcb-2024-0181","url":null,"abstract":"Pediatric infections account for approximately one-third of all deaths in children under 5 years globally. Lactoferrin (LF) supplementation has the potential to reduce infection-related morbidity due to its antimicrobial, anti-inflammatory, and immunoregulatory properties. We conducted a systematic review and meta-analysis of oral LF supplementation randomized controlled trials in population under 18 years old. The primary outcomes were infection-associated outcomes: late onset sepsis (LOS), diarrhea, and upper respiratory infections (URIs). We also analyzed mortality among LOS studies. Of 1594 citations identified, 25 studies met eligibility criteria, including 10 studies of LOS, 14 of diarrhea, and 8 of URI. LF supplementation was associated with fewer patients with culture-proven or probable neonatal LOS compared to placebo (odds ratio (OR): 0.60; 95% confidence interval (CI): 0.42-0.86), with fewer patients with diarrhea compared to placebo in children (OR: 0.56; 95% CI: 0.41-0.75), and no significant fewer patients with URI (OR: 0.61; 95% CI: 0.27-1.40). Before LF can be used as a public health intervention, it is necessary to refine some aspects of the design of future trials. Ideally these trials should be conducted in countries with the highest burden of infections, where the potential benefit is expected to have the largest impact.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-23"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Evaluation of drug-free methods for the detection of gene silencing in S. cerevisiae. 修正：酿酒葡萄球菌基因沉默检测的无药方法评价。

IF 2.1 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0363

Kholoud Shaban, Safia Mahabub Sauty, Ashley Fisher, Ashley Cheng, Krassimir Yankulov

引用次数: 0

Retraction: Active vitamin D activates chondrocyte autophagy to reduce osteoarthritis via mediating the AMPK-mTOR signaling pathway. 撤回：活性维生素D通过介导AMPK-mTOR信号通路激活软骨细胞自噬，减少骨关节炎。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0020

引用次数: 0

Srcap loss alters H2A.Z-dependent and neuronal differentiation-related gene expression in N2A cells. Srcap损失改变H2A。N2A细胞中z依赖性和神经元分化相关基因的表达。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0294

Karanveer S Johal, Sandra A Youssef, Samira M Ibrahim, Lina A Dizon-Mapula, Isabella R Galluzzo, Gilda Stefanelli

{"title":"Srcap loss alters H2A.Z-dependent and neuronal differentiation-related gene expression in N2A cells.","authors":"Karanveer S Johal, Sandra A Youssef, Samira M Ibrahim, Lina A Dizon-Mapula, Isabella R Galluzzo, Gilda Stefanelli","doi":"10.1139/bcb-2024-0294","DOIUrl":"10.1139/bcb-2024-0294","url":null,"abstract":"The chromatin remodeler SRCAP plays a critical role in depositing the histone variant H2A.Z, which is essential for transcriptional regulation, chromatin accessibility, and neurodevelopmental processes. Despite its known importance, the mechanisms by which SRCAP regulates H2A.Z dynamics during neuronal differentiation remain poorly understood. Here, we investigated the impact of Srcap knockdown on H2A.Z incorporation and transcriptional regulation in N2A cells. Chromatin immunoprecipitation revealed reduced H2A.Z occupancy at activity-dependent and neurodevelopmental genes upon Srcap knockdown, confirming Srcap's role in H2A.Z deposition. Interestingly, CBP recruitment and global histone H3 acetylation were unaffected by Srcap knockdown at steady-state conditions, suggesting an H2A.Z-specific function of Srcap. We also observed that retinoic acid-induced neuronal differentiation leads to dynamic changes in H2A.Z levels at developmental loci, which are disrupted in Srcap-deficient cells. Gene expression analysis revealed altered expression of neurodevelopmental genes in the absence of Srcap, correlating with reduced H2A.Z occupancy. Together, these findings demonstrate that Srcap is essential for regulating H2A.Z dynamics and gene expression during neuronal differentiation, offering new insights into its role in chromatin remodelling and its potential involvement in neurodevelopmental disorders.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0