Biochemistry and Cell Biology最新文献_第6页

Retraction: LncRNA MALAT1 regulates diabetic cardiac fibroblasts through the Hippo-YAP signaling pathway. 撤稿：LncRNA MALAT1通过Hippo-YAP信号通路调控糖尿病心脏成纤维细胞。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0021

引用次数: 0

Revisiting galectin-1, -3, -4, and -9 as biotargets for colorectal cancer. 重述半乳糖凝集素-1、-3、-4和-9作为结直肠癌的生物靶点。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0218

Ammar Akram Kamarudin, Nadiah Abu

引用次数: 0

Lactoferrin enhances the antibiotic treatment of Staphylococcus aureus in bone infection. 乳铁蛋白增强了金黄色葡萄球菌骨感染的抗生素治疗。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2024-0101

Jillian Cornish, Reece Joseph, Jian-Ming Lin, Stuart G Irwin, Janesha Perera, Karen E Callon, Jagir R Hassan, Jingyuan Wen, Haemish Crawford, Brya G Matthews, Nicholas N Ashton, D Williams, Heather M Baker, Eduard N Baker, Simon Swift

{"title":"Lactoferrin enhances the antibiotic treatment of Staphylococcus aureus in bone infection.","authors":"Jillian Cornish, Reece Joseph, Jian-Ming Lin, Stuart G Irwin, Janesha Perera, Karen E Callon, Jagir R Hassan, Jingyuan Wen, Haemish Crawford, Brya G Matthews, Nicholas N Ashton, D Williams, Heather M Baker, Eduard N Baker, Simon Swift","doi":"10.1139/bcb-2024-0101","DOIUrl":"https://doi.org/10.1139/bcb-2024-0101","url":null,"abstract":"Lactoferrin (Lf), we have previously shown, has therapeutic potential in the field of skeletal regenerative medicine demonstrating its potent stimulating effects on bone growth. Recently, we have identified bovine lactoferrin (bLf) as a factor that also enhances antibiotic killing of Staphylococcus aureus (S. aureus). Biofilms are associated with around 65% of all infections and 80% of chronic infections. One feature of biofilm infection is tolerance to antibiotics due to the survival of a subpopulation of biofilm bacteria, where laboratory tests on planktonic cells indicate susceptibility. Tolerance is seen in bone infections of osteomyelitis and prosthetic joints, where methicillin-susceptible S. aureus (MSSA) strains predominate, but where treatments with the frontline penicillinase-resistant antibiotic cefazolin (CEF) can be ineffective. In vitro-grown biofilms of MSSA are 1000-fold more tolerant to CEF but can be eradicated by CEF at 10x minimal inhibitory concentration in the presence of bLf. Bone infection can impede blood circulation within the bone, leading to bone death. Lf as a potent stimulator of bone growth adds to its appeal as a treatment for bone infections.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":"103 ","pages":"1-5"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How prevalent are lactoferrin receptors in Gram-negative bacteria? 乳铁蛋白受体在革兰氏阴性菌中有多普遍？

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2025-01-09 DOI: 10.1139/bcb-2024-0180

Nikolas F Ewasechko, David M Curran, Ken Yu Khaw, Anthony B Schryvers

{"title":"How prevalent are lactoferrin receptors in Gram-negative bacteria?","authors":"Nikolas F Ewasechko, David M Curran, Ken Yu Khaw, Anthony B Schryvers","doi":"10.1139/bcb-2024-0180","DOIUrl":"10.1139/bcb-2024-0180","url":null,"abstract":"Surface receptors in Gram-negative bacteria that bind and extract iron from the host glycoproteins transferrin (Tf) or lactoferrin (Lf) was discovered 35 years ago in pathogenic Neisseria species and subsequently was discovered in other pathogens of humans and food production animals. These bacterial species reside exclusively on the mucosal surfaces of the respiratory or genitourinary tract of their mammalian host and rely on their host specific Tf and Lf receptors to acquire iron for survival. Since the specificity of the bacterial Tf receptors was shown to be due to selective pressures on the host Tf, their presence in bacteria that reside in both mammals and birds indicates that they arose over 320 million years ago. Once Lf arose in mammals due to a gene duplication event, Lf receptors subsequently arose from Tf receptors. The focus on pathogens for discovery of these receptors has led to a limited understanding of how prevalent the Tf and Lf receptors are in commensal species and raises the question whether they are present in additional bacterial lineages. Since the Lf receptor provides a secondary iron acquisition system plus can provide protection from cationic peptides its presence varies in bacterial lineages.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ischemia-induced expression status of cofilin 1, CRSP2, HSP90, HSP27, and IL8 in epicardial adipose tissue and single cell transcriptomic profiling of stromal cells. 缺血诱导的心外膜脂肪组织中Cofilin 1、CRSP2、HSP90、HSP27和IL-8的表达状态以及基质细胞的单细胞转录组学分析

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 Epub Date: 2024-12-17 DOI: 10.1139/bcb-2024-0210

Ed Cha, Sung Ho Hong, Vy La, Pranav Madabhushi, Darren Teramoto, Cameron Fung, Finosh G Thankam

{"title":"Ischemia-induced expression status of cofilin 1, CRSP2, HSP90, HSP27, and IL8 in epicardial adipose tissue and single cell transcriptomic profiling of stromal cells.","authors":"Ed Cha, Sung Ho Hong, Vy La, Pranav Madabhushi, Darren Teramoto, Cameron Fung, Finosh G Thankam","doi":"10.1139/bcb-2024-0210","DOIUrl":"10.1139/bcb-2024-0210","url":null,"abstract":"Epicardial adipose tissue (EAT) is a rich source of EAT-derived stromal cells (EATDS), which possess regenerative potential. CRSP2, HSP27, IL8, HSP90, and Cofilin 1 were detected in the secretome of left ventricular stromal cells under ischemia challenge. However, the association of these genes in the EAT and EATDS remain understudied. We aim to assess the status of cofilin 1, CRSP2, HSP27, IL8, and HSP90 in the EAT of myocardial infarction (MI) and coronary artery bypass graft (CABG) swine models and in vitro stimulated ischemic EATDS. Expression status of these proteins in EAT were assessed by immunostaining, and in EATDS using qRT-PCR, immunostaining, and Western blot. EATDS phenotyping was performed using sc-RNAseq analysis. Cofilin 1 was increased while the other four genes were decreased in the CABG. IL8 and HSP90 were increased, while CRSP2, HSP27, and cofilin 1 were decreased in the MI group. Similar trend was displayed in the expression of these genes in EATDS. Additionally, EATDS displayed versatile phenotypes at single cell resolution based on the differential expression of various gene signatures. The findings revealed novel insights into EAT/EATDS biology and further understanding regarding the EATDS sub-phenotypes would open novel avenues in translational cardiology.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"1-15"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proceedings of the Canadian Society for Molecular Biosciences (CSMB) 2024 Conference - Gene Regulation: From Cells to Systems. 加拿大分子生物科学学会（CSMB） 2024年会议论文集-基因调控：从细胞到系统。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2025-01-01 DOI: 10.1139/bcb-2025-0146

Mojgan Rastegar, Hans-Joachim Wieden, James R Davie

{"title":"Proceedings of the Canadian Society for Molecular Biosciences (CSMB) 2024 Conference - Gene Regulation: From Cells to Systems.","authors":"Mojgan Rastegar, Hans-Joachim Wieden, James R Davie","doi":"10.1139/bcb-2025-0146","DOIUrl":"https://doi.org/10.1139/bcb-2025-0146","url":null,"abstract":"The 67th Canadian Society for Molecular Biosciences (CSMB) Annual Conference took place in Winnipeg, Manitoba, from 6 May to 8 May 2024, at the University of Manitoba, Winnipeg. The conference theme \"Gene Regulation: From Cells to Systems\" was selected to reflect the breadth and multidisciplinary nature of CSMB's membership and to facilitate interactions among scientists and researchers across different disciplines and with diverse research programs and approaches. This meeting attracted registered participants from provinces across Canada and the USA. The objective of this meeting was to promote the inclusive dissemination, conversation, and discussions of molecular biosciences research, in different areas of molecular biosciences covering a wide range of topics in basic science and health-related research subjects such as neuroscience, cancer biology, immunology, physiology, bacterial systems, RNA biology, protein homeostasis, cellular metabolism and cell death mechanisms, neurobiology and neurogenetics, epigenetics, chromatin biology, environmental influence, stem cell biology, and genome-wide studies. This meeting provided a discussion opportunity for molecular bioscience researchers to develop innovative and novel strategies and collaborations for biomedical and translational research.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":"103 ","pages":"1-3"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing validated RT-qPCR workflow for the analysis of oligodendrocyte gene expression in the developing murine brain. 建立有效的 RT-qPCR 工作流程，用于分析发育中鼠脑中少突胶质细胞基因的表达。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-08-08 DOI: 10.1139/bcb-2024-0088

Samantha Smith, Emma R Swan, Kendra L Furber

{"title":"Establishing validated RT-qPCR workflow for the analysis of oligodendrocyte gene expression in the developing murine brain.","authors":"Samantha Smith, Emma R Swan, Kendra L Furber","doi":"10.1139/bcb-2024-0088","DOIUrl":"10.1139/bcb-2024-0088","url":null,"abstract":"Myelination is essential for the proper conduction of impulses across neuronal networks. Mature, myelinating glia differentiate from progenitor cells through distinct stages that correspond to oligodendrocyte-specific gene expression markers. Reverse transcription quantiatative PCR (RT-qPCR) is a common technique used to quantify gene expression across cell development; however, a lack of standardization and transparency in methodology may lead to irreproducible data. Here, we have designed and validated RT-qPCR assays for oligodendrocyte genes and reference genes in the developing C57BL6/J mouse brain that align with the MIQE guidelines, including quality controls for primer specificity, temperature dependence, and efficiency. A panel of eight commonly used reference genes was ranked using a series of reference gene stability methods that consistently identified Gapdh, Sdha, Hmbs, Hprt1, and Pgk1 as the top candidates for normalization across brain regions. In the cerebrum, myelin genes peaked in expression at postnatal day 21, which corresponds to the peak of developmental myelination. The gene expression patterns from the brain homogenate were in agreement with previously reported RNA-seq and microarray profiles from oligodendrocyte lineage cells. The validated RT-qPCR assays begin to build a framework for future investigation into the molecular mechanisms that regulate myelination in mouse models of brain development, aging, and disease.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"492-505"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canada's contributions to RNA research: past, present, and future perspectives. 加拿大对核糖核酸研究的贡献：过去、现在和未来的展望。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-09-25 DOI: 10.1139/bcb-2024-0176

Eric Lécuyer, Martin Sauvageau, Ute Kothe, Peter J Unrau, Masad J Damha, Jonathan Perreault, Sherif Abou Elela, Mark A Bayfield, Julie M Claycomb, Michelle S Scott

{"title":"Canada's contributions to RNA research: past, present, and future perspectives.","authors":"Eric Lécuyer, Martin Sauvageau, Ute Kothe, Peter J Unrau, Masad J Damha, Jonathan Perreault, Sherif Abou Elela, Mark A Bayfield, Julie M Claycomb, Michelle S Scott","doi":"10.1139/bcb-2024-0176","DOIUrl":"10.1139/bcb-2024-0176","url":null,"abstract":"The field of RNA research has provided profound insights into the basic mechanisms modulating the function and adaption of biological systems. RNA has also been at the center stage in the development of transformative biotechnological and medical applications, perhaps most notably was the advent of mRNA vaccines that were critical in helping humanity through the Covid-19 pandemic. Unbeknownst to many, Canada boasts a diverse community of RNA scientists, spanning multiple disciplines and locations, whose cutting-edge research has established a rich track record of contributions across various aspects of RNA science over many decades. Through this position paper, we seek to highlight key contributions made by Canadian investigators to the RNA field, via both thematic and historical viewpoints. We also discuss initiatives underway to organize and enhance the impact of the Canadian RNA research community, particularly focusing on the creation of the not-for-profit organization RNA Canada ARN. Considering the strategic importance of RNA research in biology and medicine, and its considerable potential to help address major challenges facing humanity, sustained support of this sector will be critical to help Canadian scientists play key roles in the ongoing RNA revolution and the many benefits this could bring about to Canada.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"472-491"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The importance of prion research. 朊病毒研究的重要性

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-07-12 DOI: 10.1139/bcb-2024-0018

Shehab Eid, Seojin Lee, Claire E Verkuyl, Dustin Almanza, Joseph Hanna, Sandra Shenouda, Ari Belotserkovsky, Wenda Zhao, Joel C Watts

{"title":"The importance of prion research.","authors":"Shehab Eid, Seojin Lee, Claire E Verkuyl, Dustin Almanza, Joseph Hanna, Sandra Shenouda, Ari Belotserkovsky, Wenda Zhao, Joel C Watts","doi":"10.1139/bcb-2024-0018","DOIUrl":"10.1139/bcb-2024-0018","url":null,"abstract":"Over the past four decades, prion diseases have received considerable research attention owing to their potential to be transmitted within and across species as well as their consequences for human and animal health. The unprecedented nature of prions has led to the discovery of a paradigm of templated protein misfolding that underlies a diverse range of both disease-related and normal biological processes. Indeed, the \"prion-like\" misfolding and propagation of protein aggregates is now recognized as a common underlying disease mechanism in human neurodegenerative disorders such as Alzheimer's and Parkinson's disease, and the prion principle has led to the development of novel diagnostic and therapeutic strategies for these illnesses. Despite these advances, research into the fundamental biology of prion diseases has declined, likely due to their rarity and the absence of an acute human health crisis. Given the past translational influence, continued research on the etiology, pathogenesis, and transmission of prion disease should remain a priority. In this review, we highlight several important \"unsolved mysteries\" in the prion disease research field and how solving them may be crucial for the development of effective therapeutics, preventing future outbreaks of prion disease, and understanding the pathobiology of more common human neurodegenerative disorders.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"448-471"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141598333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathophysiological relevance and therapeutic outlook of GPR43 in atherosclerosis. 动脉粥样硬化中 GPR43 的病理生理学相关性和治疗前景。

IF 2.4 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-07-16 DOI: 10.1139/bcb-2024-0053

Mu-Yao Tang, Hao Xie, Jin-Tao Tao, Chun Zhang, Yao-Hua Luo, Cong Zhang, Si-Qin Peng, Lin-Xi Xie, Wen-Bo Lv, Chi Zhang, Liang Huang

{"title":"Pathophysiological relevance and therapeutic outlook of GPR43 in atherosclerosis.","authors":"Mu-Yao Tang, Hao Xie, Jin-Tao Tao, Chun Zhang, Yao-Hua Luo, Cong Zhang, Si-Qin Peng, Lin-Xi Xie, Wen-Bo Lv, Chi Zhang, Liang Huang","doi":"10.1139/bcb-2024-0053","DOIUrl":"10.1139/bcb-2024-0053","url":null,"abstract":"Atherosclerosis (AS) is an inflammatory arterial disorder that occurs due to the deposition of the excessive lipoprotein under the artery intima, mainly including low-density lipoprotein and other apolipoprotein B-containing lipoproteins. G protein-coupled receptors (GPCRs) play a crucial role in transmitting signals in physiological and pathophysiological conditions. GPCRs recognize inflammatory mediators, thereby serving as important players during chronic inflammatory processes. It has been demonstrated that free fatty acids can function as ligands for various GPCRs, such as free fatty acid receptor (FFAR)1/GPR40, FFAR2/GPR43, FFAR3/GPR41, FFAR4/GPR120, and the lipid metabolite binding glucose-dependent insulinotropic receptor (GPR119). This review discusses GPR43 and its ligands in the pathogenesis of AS, especially focusing on its distinct role in regulating chronic vascular inflammation, inhibiting oxidative stress, ameliorating endothelial dysfunction and improving dyslipidemia. It is hoped that this review may provide guidance for further studies aimed at GPR43 as a promising target for drug development in the prevention and therapy of AS.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":" ","pages":"418-429"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0