Biochemistry and Cell Biology最新文献_第6页

WWP2 binds to NKRF, enhances the NF-κB signaling, and promotes malignant phenotypes of acute myeloid leukemia cells. WWP2与NKRF结合，增强NF-κB信号传导，并促进急性髓系白血病细胞的恶性表型。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-02-01 Epub Date: 2023-11-03 DOI: 10.1139/bcb-2022-0360

Hongjia Wang, Xin Lian, Kexin Wang, Shuye Wang

{"title":"WWP2 binds to NKRF, enhances the NF-κB signaling, and promotes malignant phenotypes of acute myeloid leukemia cells.","authors":"Hongjia Wang, Xin Lian, Kexin Wang, Shuye Wang","doi":"10.1139/bcb-2022-0360","DOIUrl":"10.1139/bcb-2022-0360","url":null,"abstract":"Acute myeloid leukemia (AML) is one of the hematological malignancies with a high recurrence rate. WW domain-containing E3 ubiquitin protein ligase 2 (WWP2) is identified as a pivotal regulator of tumor progression. This study aimed to assess the possible role of WWP2 in AML. Analysis of the GEPIA database indicated an elevated WWP2 expression in AML. We established stable WWP2-overexpressed or WWP2-silenced cells using lentivirus loaded with cDNA encoding WWP2 mRNA or shRNA targeting WWP2. Notably, WWP2 overexpression facilitated cell proliferation and cell cycle progression, which was manifested as the increase of colony formation number, S-phase percentage and cell cycle related protein levels. As observed, WWP2 knockdown presented opposite effects, leading to inhibition of tumorigenicity. Strikingly, WWP2 knockdown induced apoptosis, accompanied by upregulation of pro-apoptosis proteins cleaved caspase-9, Bax and cleaved caspase-3 and downregulation of anti-apoptosis protein Bcl-2. Functionally, we further confirmed that WWP2 overexpression enhanced the NF-κB signaling and upregulated the levels of downstream genes, which may contribute to aggravating the development of AML. More importantly, by co-immunoprecipitation assay, we verified that WWP2 bound to NF-κB-repressing factor (NKRF) and promoted NKRF ubiquitylation. Dramatically, NKRF overexpression abolished the role of WWP2 in facilitating the process of AML. Overall, our observations confirm that WWP2 exerts a critical role in the tumorigenicity of AML, and NKRF is regarded as an essential factor in the WWP2-mediated AML progression. WWP2 may be proposed as a promising target of AML.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71420301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BioCanRx Summit for Cancer Immunotherapy 2022 Proceedings. 癌症免疫疗法BioCanRx峰会2022年论文集。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-02-01 Epub Date: 2023-10-23 DOI: 10.1139/bcb-2023-0207

Stacey N Lee, Victoria Hoskin, Céline M Laumont, Shannon Snelling, Lorenzo Lindo, Lou Bird, Vera Samarkina, Chantale Thurston, Grace Fox, Sarah Ivanco, Megan Mahoney, Jeanette E Boudreau, Sarah Nersesian

{"title":"BioCanRx Summit for Cancer Immunotherapy 2022 Proceedings.","authors":"Stacey N Lee, Victoria Hoskin, Céline M Laumont, Shannon Snelling, Lorenzo Lindo, Lou Bird, Vera Samarkina, Chantale Thurston, Grace Fox, Sarah Ivanco, Megan Mahoney, Jeanette E Boudreau, Sarah Nersesian","doi":"10.1139/bcb-2023-0207","DOIUrl":"10.1139/bcb-2023-0207","url":null,"abstract":"From 19 to 21 November 2022, BioCanRx held its first post-pandemic in-person Summit for Cancer Immunotherapy in Montreal, Canada. The meeting was well attended by patients, trainees, researchers, clinicians, and industry professionals, who came together to discuss the current state and future of biotherapeutics for cancer in Canada and beyond. Three plenaries, three keynote speakers, a lively debate, and panel discussions, together with poster sessions and a social event, made the event memorable and productive. The current state of cellular therapies, cellular engineering, clinical trials, and the role of the cancer microbiome were discussed in plenary session, and the patient voice was welcomed and present throughout the meeting, in large part due to the Learning Institute, a BioCanRx initiative to include patient partners in research. In this meeting review, we highlight the platform presentations, keynote speakers, debate combatants, panellists, and the patient perspective on the annual meeting.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between chromatin, chromosomes, and epigenetics. 染色质、染色体和表观遗传学之间的串扰。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-02-01 Epub Date: 2023-08-29 DOI: 10.1139/bcb-2023-0165

Alyssa Ialongo, Ssu-Yu Yeh, Ho Sung Rhee

引用次数: 0

Comparative analysis of the effect of hypoxia in two different tumor cell models shows the differential involvement of PTEN control of proangiogenic pathways. 对两种不同肿瘤细胞模型中低氧效应的比较分析表明，PTEN 对促血管生成途径的控制有不同程度的参与。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-02-01 Epub Date: 2023-07-17 DOI: 10.1139/bcb-2023-0047

Aleksandra Majewska, Klaudia Brodaczewska, Aleksandra Filipiak-Duliban, Claudine Kieda

{"title":"Comparative analysis of the effect of hypoxia in two different tumor cell models shows the differential involvement of PTEN control of proangiogenic pathways.","authors":"Aleksandra Majewska, Klaudia Brodaczewska, Aleksandra Filipiak-Duliban, Claudine Kieda","doi":"10.1139/bcb-2023-0047","DOIUrl":"10.1139/bcb-2023-0047","url":null,"abstract":"Hypoxia, low, non-physiological oxygen tension is a key regulator of tumor microenvironment, determining the pathological tumor vascularization. Alleviation of hypoxia through vessel normalization may be a promising therapeutic approach. We aimed to assess the role of low oxygen tension in PTEN-related pathways and proangiogenic response, in vitro, in two different tumor cell lines, focusing on potential therapeutic targets for tumor vessel normalization. Downregulation of PTEN in hypoxia mediates the activation of distinct mechanisms: cytoplasmic pAKT activation in melanoma and pMDM2 modulation in kidney cancer. We show that hypoxia-induced proangiogenic potential was stronger in Renca cells than B16 F10-confirmed by a distinct secretory potential and different ability to affect endothelial cells functions. Therefore, the impact of hypoxia on PTEN-mediated regulation may determine the therapeutic targets and effectiveness of vessel normalization and intrinsic characteristics of cancer cell have to be taken into account when designing treatment.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LINE-1: an emerging initiator of cGAS-STING signalling and inflammation that is dysregulated in disease. LINE-1：一种新出现的cGAS STING信号和炎症的引发剂，在疾病中失调。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2024-02-01 Epub Date: 2023-08-29 DOI: 10.1139/bcb-2023-0134

Sabateeshan Mathavarajah, Graham Dellaire

{"title":"LINE-1: an emerging initiator of cGAS-STING signalling and inflammation that is dysregulated in disease.","authors":"Sabateeshan Mathavarajah, Graham Dellaire","doi":"10.1139/bcb-2023-0134","DOIUrl":"10.1139/bcb-2023-0134","url":null,"abstract":"The cGAS-STING (cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING)) axis integrates DNA damage and cellular stress with type I interferon (IFN) signalling to facilitate transcriptional changes underlying inflammatory stress responses. The cGAS-STING pathway responds to cytosolic DNA in the form of double-stranded DNA, micronuclei, and long interspersed nuclear element 1 (L1) retroelements. L1 retroelements are a class of self-propagating non-long terminal repeat transposons that have remained highly active in mammalian genomes. L1 retroelements are emerging as important inducers of cGAS-STING and IFN signalling, which are often dysregulated in several diseases, including cancer. A key repressor of cGAS-STING and L1 activity is the exonuclease three prime repair exonuclease 1 (TREX1), and loss of TREX1 promotes the accumulation of L1. In addition, L1 dysregulation is a common theme among diseases with chronic induction of type I IFN signalling through cGAS-STING, such as Aicardi-Goutières syndrome, Fanconi anemia, and dermatomyositis. Although TREX1 is highly conserved in tetrapod species, other suppressor proteins exist that inhibit L1 retrotransposition. These suppressor genes when mutated are often associated with diseases characterized by unchecked inflammation that is associated with high cGAS-STING activity and elevated levels of L1 expression. In this review, we discuss these interconnected pathways of L1 suppression and their role in the regulation of cGAS-STING and inflammation in disease.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10103733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating the immune system as a therapeutic target for myelodysplastic syndromes and acute myeloid leukemia. 调节免疫系统作为骨髓增生异常综合征和急性髓系白血病的治疗靶点。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2023-12-01 Epub Date: 2023-08-11 DOI: 10.1139/bcb-2022-0374

Caroline M Putnam, Lahari Kondeti, Meredith B A Kesler, Melinda E Varney

引用次数: 0

Correction: Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells. 更正:硝基氮氧化物对PC12细胞缺氧损伤的保护作用。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2023-12-01 Epub Date: 2023-11-24 DOI: 10.1139/bcb-2023-0321

Hongbo Luo, Wei Sun, Jin Shao, Huiping Ma, Zhengping Jia, Linlin Jing

引用次数: 0

Time to treat the climate and nature crisis as one indivisible global health emergency. 是时候将气候和自然危机视为一个不可分割的全球卫生紧急事件。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2023-12-01 DOI: 10.1139/bcb-2023-0329

引用次数: 0

Automatic detection of Cryptosporidium in optical microscopy images using YOLOv5x: a comparative study. 使用YOLOv5x在光学显微镜图像中自动检测隐孢子虫：一项比较研究。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2023-12-01 Epub Date: 2023-09-26 DOI: 10.1139/bcb-2023-0059

Johan Sebastian Lopez Salguero, Melissa Rodríguez Rendón, Jessica Triviño Valencia, Jorge Andrés Cuellar Gil, Carlos Andrés Naranjo Galvis, Oscar Moscoso Londoño, César Leandro Londoño Calderón, Fabio Augusto Gonzáles Osorio, Reinel Tabares Soto

{"title":"Automatic detection of Cryptosporidium in optical microscopy images using YOLOv5x: a comparative study.","authors":"Johan Sebastian Lopez Salguero, Melissa Rodríguez Rendón, Jessica Triviño Valencia, Jorge Andrés Cuellar Gil, Carlos Andrés Naranjo Galvis, Oscar Moscoso Londoño, César Leandro Londoño Calderón, Fabio Augusto Gonzáles Osorio, Reinel Tabares Soto","doi":"10.1139/bcb-2023-0059","DOIUrl":"10.1139/bcb-2023-0059","url":null,"abstract":"Here, a machine learning tool (YOLOv5) enables the detection of Cryptosporidium microorganisms using optical and phase contrast microscope images. The two databases were processed using 520 images (optical microscopy) and 1200 images (phase contrast microscopy). It used Python libraries to label, standardize the size, and crop the images to generate the input tensors to the YOLOv5 network (s, m, and l). It implemented two experiments using randomly initialized weights in optical and phase contrast microscope images. The other two experiments used the parameters for the best training time obtained before and after retraining the models. Metrics used to assess model accuracy were mean average accuracy, confusion matrix, and the F1 scores. All three metrics confirmed that the optimal model used the best epoch of optical imaging training and retraining with phase contrast imaging. Experiments with randomly initialized weights with optical imaging showed the lowest precision for Cryptosporidium detection. The most stable model was YOLOv5m, with the best results in all categories. However, the differences between all models are lower than 2%, and YOLOv5s is the best option for Cryptosporidium detection considering the differences in computational costs of the models.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10367400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NDUFA4 promotes the progression of head and neck paraganglioma by inhibiting ferroptosis. NDUFA4通过抑制铁下垂促进头颈部副神经节瘤的进展。

IF 2.9 3区生物学

Biochemistry and Cell Biology Pub Date : 2023-12-01 Epub Date: 2023-08-21 DOI: 10.1139/bcb-2023-0018

Zhigang Wang, Erxing Tao, Yiming Chen, Qi Wang, Min Liu, Liang Wei, Siyi Xu, Wei Chen, Chunlong Zhong

{"title":"NDUFA4 promotes the progression of head and neck paraganglioma by inhibiting ferroptosis.","authors":"Zhigang Wang, Erxing Tao, Yiming Chen, Qi Wang, Min Liu, Liang Wei, Siyi Xu, Wei Chen, Chunlong Zhong","doi":"10.1139/bcb-2023-0018","DOIUrl":"10.1139/bcb-2023-0018","url":null,"abstract":"NDUFA4 is a component of respiratory chain-oxidative phosphorylation pathway. NDUFA4 is highly expressed in tumor tissues, but little is known about the function of NDUFA4 in head and neck paraganglioma (HNPGL). We examined NDUFA4 expression in tissues from 10 HNPGL patients and 6 controls using qRT-PCR and Western blotting. NDUFA4 knockdown PGL-626 cells were established by using lentivirus infection and puromycin screening. Cell viability, ATP production, lipid reactive oxygen species, and mitochondrial membrane potential assays were performed to investigate the ferroptotic effects in NDUFA4 deficiency HNPGL cancer cells. Xenograft mouse model was created to detect the synergetic antitumor action between NDUFA4 deficiency and Metformin. NDUFA4 was upregulated in tumor tissues of HNPGL patients. NDUFA4 knockdown impaired the assembly of mitochondrial respiratory chain complexes and decreased the production of ATP and reduced cancer cell viability. Mechanistically, NDUFA4 knockdown increased cell ferroptosis, which further promoted Metformin-induced ferroptosis in PGL-626 cells. Therefore, NDUFA4 deficiency enhanced Metformin-mediated inhibition of the HNPGL progression in mice. In conclusion, NDUFA4 promotes the progression of HNPGL, and NDUFA4 knockdown enhances Metformin-mediated inhibition of the HNPGL progression in a mouse model.","PeriodicalId":8775,"journal":{"name":"Biochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0