BioChip Journal最新文献_第2页

Development of an Electrochemical Biosensor for Tetrodotoxin Using Specific Binding Peptide on Polypyrrole/Au Nanoparticle-Modified Electrodes 利用聚吡咯/金纳米粒子修饰电极上的特异性结合肽开发河豚毒素电化学生物传感器

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-07-22 DOI: 10.1007/s13206-024-00162-2

Su Min Kim, Ping Xu, Moon Seop Hyun, Jong Pil Park, Chan Yeong Park, Tae Jung Park

{"title":"Development of an Electrochemical Biosensor for Tetrodotoxin Using Specific Binding Peptide on Polypyrrole/Au Nanoparticle-Modified Electrodes","authors":"Su Min Kim, Ping Xu, Moon Seop Hyun, Jong Pil Park, Chan Yeong Park, Tae Jung Park","doi":"10.1007/s13206-024-00162-2","DOIUrl":"https://doi.org/10.1007/s13206-024-00162-2","url":null,"abstract":"The concern regarding tetrodotoxin (TTX), a highly hazardous marine neurotoxin found in puffer fish, has expanded beyond Asia due to the migration of puffer fish caused by the rise in global temperatures. This highlights the urgent need to develop fast yet reliable methods for detecting TTX. In this study, we developed a peptide-based potentiometric TTX sensor based on a polypyrrole/Au nanoparticle-modified carbon screen-printed electrode (PPy/AuNP SPE). The bioreceptor responsible for recognizing TTX is a specific binding peptide that was discovered through phage display technique. The phage-displayed peptide candidates were sorted based on frequency and similarity, and their binding affinity was subsequently assessed via phage enzyme-linked immunosorbent assay. The C-terminal of the specific binding peptide was then modified with cysteamine to facilitate its immobilization through Au–S bonding on the PPy/AuNP SPE platform, thereby constructing the TTX sensor. The sensing platform was prepared by successive electrodeposition of polypyrrole and AuNP onto the surface of carbon SPE as a substrate. Both materials play significant roles to improve the poor conductivity of carbon SPE and provide sufficient immobilization sites for TTX receptors, respectively. Finally, the PPy/AuNP TTX sensor demonstrated a detection limit of around 2.80 ppb with a detection range from 2 to 1000 ppb, making it a promising platform for rapid and reliable marine toxin detection.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"48 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141744745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Electrochemical Immuno-cytosensor Modified with Nanofibers for the Determination of a Carcinoembryonic Antigen 用纳米纤维修饰的电化学免疫细胞传感器用于检测癌胚抗原

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-07-10 DOI: 10.1007/s13206-024-00159-x

Zehra Yildizbakan, Derya Bal Altuntaş, Hatice Sevim Nalkiran, Sema Aslan, Atilla Eren Mamuk, Çağdaş Koçak, Şeymanur Kurt, İhsan Nalkiran, Çiğdem Yener, C. Gökhan Ünlü

{"title":"An Electrochemical Immuno-cytosensor Modified with Nanofibers for the Determination of a Carcinoembryonic Antigen","authors":"Zehra Yildizbakan, Derya Bal Altuntaş, Hatice Sevim Nalkiran, Sema Aslan, Atilla Eren Mamuk, Çağdaş Koçak, Şeymanur Kurt, İhsan Nalkiran, Çiğdem Yener, C. Gökhan Ünlü","doi":"10.1007/s13206-024-00159-x","DOIUrl":"https://doi.org/10.1007/s13206-024-00159-x","url":null,"abstract":"In this study, La0.25Fe0.75FeO3 (PNp)perovskite nanoparticle was synthesized using the sol–gel method. PNp-coated polyacrylonitrile (PAN) nanofibers were prepared by electrospinning on the pencil graphite electrode (PGE) surface. In another step, carcinoembryonic antigen (CEA) was loaded with CEA antibodies (Anti-CEA) as a biomarker receptor. Finally, PGE/PAN@PNp/Anti-CEA was used for CEA detection. Optimization steps and cell culture steps were performed using differential pulse voltammetry (DPV). The use of this composite system is a novel immunosensor development approach for label-free detection of CEA. Under optimum conditions, detection limit (LOD) of PGE/PAN@PNp/Anti-CEA immunosensor LOD 1.48 ng/mL, limit of quantification (LOQ) = 4.94 ng/mL, reproducibility 1.46% (n = 5) and R2 = 0.9984 for antigen concentration within a linear working range of 0.1–10 ng/mL. Also, immunosensor recovery in real serum samples containing dopamine and ascorbic acid was found as 98.94 ± 7.43. It has great potential in clinical screening of different cancer biomarkers. The number of cells attached to the PGE/PAN@PNp/Anti-CEA/BSA(bovine serum)/CEA surface decreased in RT-4(bladder cancer), MDA-MB-231 (triple-negative breast adenocarcinoma cell line), and T98G cells (glioblastoma multiforme cell line), which are known as CEA-negative cell lines, whereas the number of MCF-7 cells (estrogen-sensitive human breast cancer cell line, known to be CEA positive) attached to the PGE/PAN@PNp/Anti-CEA/BSA/CEA surface increased, indicating higher affinity to the immunosensor surface. As a result, while MCF-7, which is CEA positive, can be determined best when using an immune-cytosensor, the cell that can be best determined with cytosensors was found to be RT-4.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"35 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Immunoassay Using Diffusion Layer Dispersion Effect of Electrodes 利用电极的扩散层分散效应进行电化学免疫测定

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-07-08 DOI: 10.1007/s13206-024-00163-1

Eunyoung Kim, Jun-Hee Park, Bong-Ki Ryu, Ga-Yeon Lee

{"title":"Electrochemical Immunoassay Using Diffusion Layer Dispersion Effect of Electrodes","authors":"Eunyoung Kim, Jun-Hee Park, Bong-Ki Ryu, Ga-Yeon Lee","doi":"10.1007/s13206-024-00163-1","DOIUrl":"https://doi.org/10.1007/s13206-024-00163-1","url":null,"abstract":"Diffusion layer dispersed electrodes were presented for application in electrochemical immunoassays. The diffusion layer refers to the region on the electrode surface where concentration gradients occur during the redox reactions of the target analytes. To control the diffusion layer, electrodes of the same area and size were fabricated, and the shape of the diffusion layer was controlled by adjusting each electrode distance. To estimate the properties of the electrodes, cyclic voltammetry (CV) analysis was performed using ferricyanide ([Fe(CN)6–3]) and 3,3′5,5′-tetramethylbenzidine (TMB) as a model redox couple. Changes in the diffusion layer shape according to electrode distance were compared through simulation. The redox currents for the target analytes increased as electrode distance increased. These results indicate that even if the electrode shape and area are the same, dispersing the diffusion layer by adjusting the distance between the electrodes can affect the microelectrode characteristics. Finally, the diffusion layer dispersed electrode was applied for the medical diagnosis of the human hepatitis B virus (hHBV) antigen, and the human hepatitis C virus (hHCV) antibody was compared with conventional assay methods, such as TMB-based chromogenic detection.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"43 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141571216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Overlimiting Current in a Three-Dimensional Hierarchical Micro/Nanofluidic System by Non-uniform Compartmentalization 通过非均匀区隔增强三维分层微/纳流体系统中的超限电流

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-07-08 DOI: 10.1007/s13206-024-00161-3

Hyungjoo Park, Misun Kim, Seunghyun Kang, Taewan Kim, Sehyuk Yoon, Jihee Park, Sungjae Ha, Sung Jae Kim

{"title":"Enhancement of Overlimiting Current in a Three-Dimensional Hierarchical Micro/Nanofluidic System by Non-uniform Compartmentalization","authors":"Hyungjoo Park, Misun Kim, Seunghyun Kang, Taewan Kim, Sehyuk Yoon, Jihee Park, Sungjae Ha, Sung Jae Kim","doi":"10.1007/s13206-024-00161-3","DOIUrl":"https://doi.org/10.1007/s13206-024-00161-3","url":null,"abstract":"Overlimiting current (OLC) is a non-linear current response that occurs related to an ion concentration polarization (ICP) phenomenon in micro/nanofluidic systems and holds great importance since it represents the rate of selective ion transportation through perm-selective structure. For last two decades, numerous studies of OLC have been reported about understanding the fundamentals of nanoelectrokinetics and enhancing ion transportation through perm-selective membranes. Recent study reported that the alignment of non-uniform microspace near the perm-selective membranes in two-dimensional micro/nanofluidic systems can significantly enhance OLC, i.e., overlimiting conductance (σOLC). This is attributed to recirculation flow induced by combination of unbalanced electroosmosis and induced pressure driven flow among non-uniform microspaces. However, 2D micro/nanofluidic systems have limited practicality due to their small volume and low throughput. Herein, we tested the OLC enhancement using 3D-printed hierarchical micro/nanofluidic systems with respect to the non-uniformity of microspaces. The 3D microspaces were fabricated as a mesh structure using a conventional 3D printer. By comparing current–voltage measurement with each type of mesh, we experimentally confirmed the generation of recirculation flow among non-uniform meshes and ionic current enhancement in 3D hierarchical micro/nanofluidic system. Also, we further investigated the enhancement of overlimiting conductance depending on the mesh pattern. Furthermore, we validated that this effect of microscale non-uniform compartmentalization, both increasing surface area and aligning non-uniform spaces, appears not only at low molar concentration but at high molar concentrations. This demonstration can offer a strategy to design optimal electrochemical systems where a perm-selective ion transportation is crucial.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"40 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141570978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in Kidney-on-Chip: Antibiotic-Induced Kidney Injury and Future Directions 片上肾脏的进展：抗生素引起的肾损伤和未来发展方向

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-06-27 DOI: 10.1007/s13206-024-00160-4

Min-Hyeok Kim, Yugyeong Lee, Gwang Myeong Seo, Sungsu Park

引用次数: 0

Deep Learning-Based Automated Analysis of NK Cell Cytotoxicity in Single Cancer Cell Arrays 基于深度学习的单个癌细胞阵列中 NK 细胞细胞毒性自动分析

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-06-27 DOI: 10.1007/s13206-024-00158-y

Dowon Moon, Seong-Eun Kim, Chuangqi Wang, Kwonmoo Lee, Junsang Doh

引用次数: 0

Recent Developments in MXene-Based Enzyme-Free Electrochemical Glucose Sensing 基于 MXene 的无酶电化学葡萄糖传感技术的最新进展

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-06-25 DOI: 10.1007/s13206-024-00157-z

Phan Gia Le, Sungbo Cho

{"title":"Recent Developments in MXene-Based Enzyme-Free Electrochemical Glucose Sensing","authors":"Phan Gia Le, Sungbo Cho","doi":"10.1007/s13206-024-00157-z","DOIUrl":"https://doi.org/10.1007/s13206-024-00157-z","url":null,"abstract":"Diabetes mellitus is now on the rise worldwide, drawing a lot of attention from scientists. Well-uncontrolled diabetes leads to many serious diseases, including cardiovascular, ophthalmic, and nephrotic. It also caused 6.7 million deaths by 2021 and put a strain on the healthcare system. Therefore, developing useful methods for precisely diagnosing the current glucose concentration can serve as a gold standard for future treatment of diabetes. The classification of electrochemical glucose sensors with and without enzymes has been researched and developed. Among them, the use of enzymes in sensor fabrication results in high costs and easy denaturation in extreme conditions, whereas an enzyme-free approach can overcome these disadvantages and extend life expectancy. To support this, diverse nanomaterials were used, including MXene as an emerging material with favorable physiochemical properties that can be adapted for nonenzymatic electrochemical glucose sensor fabrication. In this study, diabetic disease, synthesis and application of MXene, and electrochemical biosensor were discussed. The application of MXene was thoroughly researched using recent representative publications. Conclusions and prospects for the MXene-based nonenzymatic electrochemical sensor are also explored and discussed.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"81 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141503421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergetic Chemo-Mechano Antimicrobial Puncturable Nanostructures for Efficient Bioaerosol Removal 高效去除生物气溶胶的协同化学机械抗菌可穿刺纳米结构

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-06-03 DOI: 10.1007/s13206-024-00156-0

Kyeong Seok Kim, Inae Lee, Joonseok Lee

{"title":"Synergetic Chemo-Mechano Antimicrobial Puncturable Nanostructures for Efficient Bioaerosol Removal","authors":"Kyeong Seok Kim, Inae Lee, Joonseok Lee","doi":"10.1007/s13206-024-00156-0","DOIUrl":"https://doi.org/10.1007/s13206-024-00156-0","url":null,"abstract":"Concerns regarding air pollution and the risk of infectious diseases caused by bioaerosols have led to growing demand for effective filtration solutions. The effective capture of bioaerosols is essential; however, addressing the potential multiplication of captured aerosols on the air filter over time is also crucial. Therefore, the development of air filters with enhanced antimicrobial protection is imperative for preventing the proliferation of bioaerosols and ensuring safer and healthier air filtration systems. In this study, antimicrobial peptides conjugated nanostructures were used to enhance the capture and killing of bioaerosols with synergistic chemo-mechano-antimicrobial actions. We designed a puncturable nanostructure on an air filter and functionalized it with the antimicrobial LL-37 peptide. Bioaerosol filtration and antimicrobial performance tests were conducted to evaluate the synergistic effects of the antimicrobial puncturable nanostructures on bioaerosol removal. The peptide-conjugated puncturable nanostructured air filter outperformed bare air filters in bioaerosol capture and exhibited significantly better contact-killing properties against bioaerosols. These attributes indicate the ability of the filter to significant capture and kill airborne pathogens. The synergetic chemo-mechano-antimicrobial nanostructured filter is promising for combating airborne threats and serves as a safe filtration solution that is free from biotoxicity concerns and is suitable for widespread implementation in the market.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"19 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141254108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling 微观生理系统作为器官特异性体外血管模型用于疾病建模

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-05-14 DOI: 10.1007/s13206-024-00152-4

Ungsig Nam, Seokhun Lee, Ashfaq Ahmad, Hee-gyeong Yi, Jessie S. Jeon

{"title":"Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling","authors":"Ungsig Nam, Seokhun Lee, Ashfaq Ahmad, Hee-gyeong Yi, Jessie S. Jeon","doi":"10.1007/s13206-024-00152-4","DOIUrl":"https://doi.org/10.1007/s13206-024-00152-4","url":null,"abstract":"The vascular system, essential for human physiology, is vital for transporting nutrients, oxygen, and waste. Since vascular structures are involved in various disease pathogeneses and exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific vascular models. While animal models possess sophisticated vascular morphologies, they exhibit significant discrepancies from human tissues due to species differences, which limits their applicability. To overcome the limitations arising from these discrepancies and the oversimplification of 2D dish cultures, microphysiological systems (MPS) have emerged as a promising alternative. These systems more accurately mimic the human microenvironment by incorporating cell interactions, physical stimuli, and extracellular matrix components, thus facilitating enhanced tissue differentiation and functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model and patient responses. This review focuses on recent advancements in MPS, particularly in modeling the human organ-specific vascular system, and discusses their potential in biological adaptation.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"36 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140932107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-Site Food Toxin Detection System Using a Gb3-Immobilized Microchip and a Portable Fluorescence Sensing System 使用 Gb3 固定化微芯片和便携式荧光传感系统的现场食品毒素检测系统

IF 4.3 3区工程技术

BioChip Journal Pub Date : 2024-05-13 DOI: 10.1007/s13206-024-00155-1

Keying Li, Jeongtae Kim, Yujin Jeong, Moo-Seung Lee, Chiwan Koo

{"title":"On-Site Food Toxin Detection System Using a Gb3-Immobilized Microchip and a Portable Fluorescence Sensing System","authors":"Keying Li, Jeongtae Kim, Yujin Jeong, Moo-Seung Lee, Chiwan Koo","doi":"10.1007/s13206-024-00155-1","DOIUrl":"https://doi.org/10.1007/s13206-024-00155-1","url":null,"abstract":"Shiga toxin (Stx) is one of the most potent bacterial toxins known to cause serious gastrointestinal disease in humans. However, the high costs in terms of time, equipment, and operators hinder their further application in timely diagnosis. In this work, we provide a method of coating globotriaosylceramide (Gb3), an Stx receptor, on a glass chip to detect food toxins in real time and a portable fluorescence detection system that can diagnose Stx after loading the chip. The total size of the system with an organic light-emitting diode (OLED) display is about 8.8 × 6.2 × 15 cm3 and its weight is about 375.5 g with a lithium-ion battery. The novel solvent-assisted lipid bilayer method was utilized to coat a bilayer of receptors on the glass substrate instead of placing a bi-lipid membrane and inserting receptors into the membrane, thereby increasing the fluorescence intensity within the chip and being detected by the system. The optimal conditions for coating receptors were verified and the flow rate of 3 μL/min and flow time of 10 min for the process of solvent exchange showed relatively good efficiency. The limit of detection was 250 pg/mL of Stx1B and the system successfully detected Stx in the lettuce eluate sample. The stability of the chip remained highly reliable within 3 days of storage, but after 14 days, the chip lost most of its detection ability due to delamination of the coated receptor bilayer.","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"23 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140932106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0