Electrochemical Detection of S-RBD Protein for Point-of-Care SARS-CoV-2 Monitoring Using Platinum-Black-Based Sensor Array

IF 5.5 3区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Zeeshan, Sathish Panneer Selvam, Jaehwan Park, Sungho Park, Hee-Eun Kim, Sungbo Cho
{"title":"Electrochemical Detection of S-RBD Protein for Point-of-Care SARS-CoV-2 Monitoring Using Platinum-Black-Based Sensor Array","authors":"Zeeshan, Sathish Panneer Selvam, Jaehwan Park, Sungho Park, Hee-Eun Kim, Sungbo Cho","doi":"10.1007/s13206-024-00153-3","DOIUrl":null,"url":null,"abstract":"<p>The COVID-19 pandemic has led to a substantial increase in the advancement of point-of-care (POC) diagnostic tools due to their potential utility in detecting and managing the spread of the disease. Currently, many diagnostic techniques necessitate advanced laboratory equipment and specialized expertise to deliver dependable, cost-effective, specific, and sensitive POC tests for COVID-19 diagnosis. Herein, we report a highly sensitive electrochemical sensor array that features S-RBD protein, covalently anchored on the surface-engineered Pt-black-coated microdisk gold electrodes to monitor severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Computer simulations were performed using different electrode gaps to optimize and fabricate the gold microdisk electrode array. The high sensitivity was ensured by decreasing the electrode gap as well as by depositing Pt-black nanoparticles on the microdisk gold electrodes, by means of chronopotentiometry. The electrical readout depends on monitoring changes in the cyclic voltammograms at the electrode/electrolyte interface as a result of the competitive interaction between monoclonal COVID-19 antibodies and varying antigen concentrations. Overall, the developed electrochemical sensor array exhibits promising electroanalytical capabilities by displaying an excellent linear response ranging from 100 to 1 µg/ml with a detection limit of ~ (0.23 ng/ml). In addition, as a proof-of-concept application, the developed electrochemical sensor array was employed as a sensing platform for the detection of heat-inactivated SARS-CoV-2. Such accomplishments highlight the advantages of low-cost localized electronic devices with high sensitivity and rapid multiple samples detection capabilities to play a crucial role in controlling the spread of infectious diseases like COVID-19.</p>","PeriodicalId":8768,"journal":{"name":"BioChip Journal","volume":"3 1","pages":""},"PeriodicalIF":5.5000,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioChip Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13206-024-00153-3","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

The COVID-19 pandemic has led to a substantial increase in the advancement of point-of-care (POC) diagnostic tools due to their potential utility in detecting and managing the spread of the disease. Currently, many diagnostic techniques necessitate advanced laboratory equipment and specialized expertise to deliver dependable, cost-effective, specific, and sensitive POC tests for COVID-19 diagnosis. Herein, we report a highly sensitive electrochemical sensor array that features S-RBD protein, covalently anchored on the surface-engineered Pt-black-coated microdisk gold electrodes to monitor severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Computer simulations were performed using different electrode gaps to optimize and fabricate the gold microdisk electrode array. The high sensitivity was ensured by decreasing the electrode gap as well as by depositing Pt-black nanoparticles on the microdisk gold electrodes, by means of chronopotentiometry. The electrical readout depends on monitoring changes in the cyclic voltammograms at the electrode/electrolyte interface as a result of the competitive interaction between monoclonal COVID-19 antibodies and varying antigen concentrations. Overall, the developed electrochemical sensor array exhibits promising electroanalytical capabilities by displaying an excellent linear response ranging from 100 to 1 µg/ml with a detection limit of ~ (0.23 ng/ml). In addition, as a proof-of-concept application, the developed electrochemical sensor array was employed as a sensing platform for the detection of heat-inactivated SARS-CoV-2. Such accomplishments highlight the advantages of low-cost localized electronic devices with high sensitivity and rapid multiple samples detection capabilities to play a crucial role in controlling the spread of infectious diseases like COVID-19.

利用基于铂-黑的传感器阵列电化学检测 S-RBD 蛋白,用于护理点 SARS-CoV-2 监测
由于 COVID-19 大流行在检测和控制疾病传播方面的潜在作用,护理点 (POC) 诊断工具的发展有了大幅提高。目前,许多诊断技术都需要先进的实验室设备和专业知识,才能为 COVID-19 诊断提供可靠、经济、特异和灵敏的 POC 检测。在此,我们报告了一种高灵敏度的电化学传感器阵列,该阵列以 S-RBD 蛋白为特征,共价锚定在表面工程化的铂-黑涂层微盘金电极上,用于监测严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)。利用不同的电极间隙进行了计算机模拟,以优化和制造微盘金电极阵列。通过减小电极间隙以及在微盘金电极上沉积铂黑纳米粒子,利用时差法确保了高灵敏度。电读数取决于监测电极/电解质界面上循环伏安图的变化,这是单克隆 COVID-19 抗体与不同浓度抗原之间竞争性相互作用的结果。总之,所开发的电化学传感器阵列具有良好的电分析能力,在 100 至 1 µg/ml 范围内具有出色的线性响应,检测限为 ~ (0.23 ng/ml)。此外,作为概念验证应用,所开发的电化学传感器阵列被用作检测热灭活 SARS-CoV-2 的传感平台。这些成果凸显了具有高灵敏度和快速多样品检测能力的低成本本地化电子设备在控制 COVID-19 等传染病传播方面的优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
BioChip Journal
BioChip Journal 生物-生化研究方法
CiteScore
7.70
自引率
16.30%
发文量
47
审稿时长
6-12 weeks
期刊介绍: BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信