Nucleic acids symposium series (2004)最新文献_第6页

Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae. 酿酒酵母tRNA成熟各阶段修饰状态的精确分析。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp151

Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki

{"title":"Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae.","authors":"Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki","doi":"10.1093/nass/nrp151","DOIUrl":"https://doi.org/10.1093/nass/nrp151","url":null,"abstract":"Transfer RNAs (tRNAs) are decorated with various post-transcriptional modifications which are enzymatically introduced at various stages of maturation. It is known that eukaryotic tRNAs are modified both in nucleus and cytoplasm. However, the order of tRNA modifications remains to be investigated. To unveil the precise timing of each modification associated with tRNA processing, we isolated precursor forms of Saccharomyces cerevisiae tRNAs at different stages of maturation, and analyzed their primary structures including modifications by mass spectrometry. The primary transcript of tRNA(Ile) was isolated from the tRNA fraction co-precipitated with Lhp1p, a yeast homolog of La protein. The precursor tRNA(Ile) without 3'-trailer sequence was isolated from the expression controllable strain when RNase P was transiently inactivated. Mass spectrometric analyses of these tRNAs revealed that many modifications were fully introduced at the stage of primary transcript, however, some modifications were partially introduced.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"301-2"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28475071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Physiological role of RsgA in ribosome biosynthesis. RsgA在核糖体生物合成中的生理作用。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp154

Yoichi Hase, Shinichiro Yokoyama, Takatugu Kimura, Shimon Goto, Akira Muto, Hyouta Himeno

引用次数: 1

Rational design and synthesis of ansa-adenosines as potential antitumor agents. 潜在抗肿瘤药物ansa-腺苷的合理设计与合成。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp003

Kazuhiro Muranaka, Satoshi Ichikawa, Akira Matsuda

引用次数: 0

Fluorescent properties of acridonyl group in DNA duplex. DNA双链中吖啶酮基的荧光性质。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp069

Masayasu Kuwahara, Atsushi Kobayashi, Masayoshi Tajima, Shunsuke Kitazume, Keisuke Anahara, Hiroaki Ozaki, Seiji Tobita

引用次数: 0

Thymidylate kinase: the lost chemotherapeutic target. 胸苷激酶:丢失的化疗靶点。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp142

Mahmoud Kandeel, Aya Kato, Yoshiaki Kitamura, Yukio Kitade

引用次数: 9

Toward a reciprocal evolution system between RNA and peptides as an artificial model for the early RNP world. 迈向RNA和多肽之间的相互进化系统，作为早期RNP世界的人工模型。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp017

Yoshiya Ikawa, Hiroyuki Furuta, Kohei Yamashita, Norimasa Kashiwagi

引用次数: 0

The exceptional properties of Plasmodium deoxyguanylate pathways as a potential area for metabolic and drug discovery studies. 脱氧鸟苷疟原虫途径的特殊性质作为代谢和药物发现研究的潜在领域。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp020

Mahmoud Kandeel, Yoshiaki Kitamura, Yukio Kitade

引用次数: 15

Development of new DNA triplex triads by using 5-substituted deoxycytidine. 用5-取代脱氧胞苷制备新的DNA三联体。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp082

Takashi Kanamori, Hirosuke Tsunoda, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio

引用次数: 0

Screening of amber suppressor tRNAs suitable to introduce nonnatural amino acids into proteins by real-time monitoring of cell-free translation. 通过实时监测无细胞翻译筛选适合将非天然氨基酸引入蛋白质的琥珀色抑制trna。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp149

Issei Iijima, Takahiro Hohsaka

{"title":"Screening of amber suppressor tRNAs suitable to introduce nonnatural amino acids into proteins by real-time monitoring of cell-free translation.","authors":"Issei Iijima, Takahiro Hohsaka","doi":"10.1093/nass/nrp149","DOIUrl":"https://doi.org/10.1093/nass/nrp149","url":null,"abstract":"Incorporation of nonnatural amino acids into proteins is a useful technique to analyze protein structure and function. We have reported that amber suppressor tRNAs suitable for efficient and specific incorporation of nonnatural amino acids into proteins can be obtained by screening a wide variety of naturally occurring tRNAs in an E. coli. cell-free translation system. The amber suppressor activity of the tRNAs was evaluated by incorporation of a fluorescent nonnatural amino acid and fluorescent SDS-PAGE analysis of cell-free translation products, though the SDS-PAGE was troublesome and time-consuming. In this research, we developed an alternative method for the screening of amber suppressor tRNAs by real-time measurement of fluorescence resonance energy transfer (FRET) from GFP to BODIPY558-linked nonnatural amino acid, which was incorporated into the N-terminus of GFP by amber suppressor tRNAs. Using this method, we demonstrated that the screening of the amber suppressor activity of various prokaryotic Trp tRNAs was performed in a high-throughput manner.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"297-8"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28475069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of yeast (m2G10) methyltransferase (Trm11 and Trm112 complex) in a wheat germ cell-free translation system. 小麦无生殖细胞翻译系统中酵母(m2G10)甲基转移酶(Trm11和Trm112复合体)的产生

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp152

Kazuki Okada, Yuki Muneyoshi, Yaeta Endo, Hiroyuki Hori

引用次数: 13