{"title":"Fluorescent properties of acridonyl group in DNA duplex.","authors":"Masayasu Kuwahara, Atsushi Kobayashi, Masayoshi Tajima, Shunsuke Kitazume, Keisuke Anahara, Hiroaki Ozaki, Seiji Tobita","doi":"10.1093/nass/nrp069","DOIUrl":null,"url":null,"abstract":"<p><p>We designed and synthesized a nucleoside derivative in which the nucleobase is replaced with acridone. The nucleoside derivative was incorporated into an oligodeoxyribonucleotide (ODN), and its influence on the stability of ODN hybrids and its fluorescent properties in a DNA duplex were measured by thermodynamic analysis and fluorescent spectroscopy. These results showed that the acridonyl group could distinguish the type of nucleobase paired from fluorescent intensity, although the hybrid stability did not depend significantly on the types of nucleobases paired.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"137-8"},"PeriodicalIF":0.0000,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp069","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids symposium series (2004)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/nrp069","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We designed and synthesized a nucleoside derivative in which the nucleobase is replaced with acridone. The nucleoside derivative was incorporated into an oligodeoxyribonucleotide (ODN), and its influence on the stability of ODN hybrids and its fluorescent properties in a DNA duplex were measured by thermodynamic analysis and fluorescent spectroscopy. These results showed that the acridonyl group could distinguish the type of nucleobase paired from fluorescent intensity, although the hybrid stability did not depend significantly on the types of nucleobases paired.
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