Nucleic acids symposium series (2004)最新文献_第5页

Aptazyme-based biosensors using a eukaryotic cell-free translation system. 利用真核生物无细胞翻译系统的适体酶生物传感器。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp131

Atsushi Ogawa

{"title":"Aptazyme-based biosensors using a eukaryotic cell-free translation system.","authors":"Atsushi Ogawa","doi":"10.1093/nass/nrp131","DOIUrl":"https://doi.org/10.1093/nass/nrp131","url":null,"abstract":"I have constructed a novel aptazyme-based biosensor system for detecting cofactors of the aptazymes using a cell-free luciferase synthesis in wheat germ extract. In this system, the activity of the aptazyme that is fused to a 5'-untranslated region of a luciferase gene can be detected as luciferase expression. In translating the aptazyme-fused mRNA as-is using a wheat germ cell-free translation system, the luciferase is almost not expressed because of the following triple suppression effects: (1) 5'-terminal three bases and (2) 5'-terminal duplex prevent the ribosome from binding to own mRNA; (3) if the ribosome binds, translation of a mimic gene in the aptazyme inhibits that of the downstream luciferase gene (OFF state). In contrast, in the presence of the aptazyme cofactor, the aptazyme in mRNA is self-cleaved to produce an aptazyme-free luciferase gene, which is translated efficiently (ON state). The ON/OFF efficiency and the detection limit of the aptazyme-based biosensor for theophylline are much higher and lower, respectively, compared to those of previously-reported one that utilizes a prokaryotic translation system.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"261-2"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28397577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional DNA nanostructures constructed by folding of multiple rectangles. 通过折叠多个矩形构建三维DNA纳米结构。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp041

Masayuki Endo, Hiroshi Sugiyama

引用次数: 4

Photo-controllable aptamer. Photo-controllable适体。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp098

Shinzi Ogasawara, Mizuo Maeda

引用次数: 3

NMR studies of DNA recognition mechanism of HMGB1 protein. HMGB1蛋白DNA识别机制的核磁共振研究。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp045

Kyoko Furuita, Shunpei Murata, Jungoo Jee, Satoshi Ichikawa, Akira Matsuda, Chojiro Kojima

{"title":"NMR studies of DNA recognition mechanism of HMGB1 protein.","authors":"Kyoko Furuita, Shunpei Murata, Jungoo Jee, Satoshi Ichikawa, Akira Matsuda, Chojiro Kojima","doi":"10.1093/nass/nrp045","DOIUrl":"https://doi.org/10.1093/nass/nrp045","url":null,"abstract":"A 2'-deoxyuridylate dimer cyclized via cross-linkage by an ethylene (U(et)(p)U) or a propylene (U(pr)(p)U) linker at the 5-position was incorporated into DNA oligomers. Fluorescence resonance energy transfer (FRET) experiments showed that they bent at approximately 90 degrees . We investigated binding abilities of U(et)(p)U and U(pr)(p)U DNA oligomers to HMGB1 A-box protein, which specifically binds to bent DNA, using nuclear magnetic resonance (NMR) spectroscopy. Both DNA oligomers bind to HMGB1 A-box protein, however, the U(et)(p)U DNA oligomer has higher affinity than the U(pr)(p)U DNA oligomer. In order to explain this difference, we studied the solution structures of the U(et)(p)U and U(pr)(p)U DNA oligomers using NMR. Most (1)H signals except for 4', 5' and 5'' were assigned. Cross-peak patterns of (1)H-(1)H NOESY spectra indicate that both oligomers have right-handed B-form like structures and the cyclization in 2'-deoxyuridylates does not break Watson-Crick base pairs. Chemical shift differences between these two DNA oligomers suggest the presence of the local structural differences in the region of 2'-deoxyuridylate dimer and its 3' side between the U(et)(p)U and U(pr)(p)U DNA oligomers.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"89-90"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28397562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Monitoring biological interactions using perforated evanescent-field-coupled waveguide-mode nanobiosensors. 利用穿孔倏逝场耦合波导模式纳米生物传感器监测生物相互作用。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp047

Subash C B Gopinath, Koichi Awazu, Makoto Fujimaki, Junji Tominaga, Kailash C Gupta, Penmetcha K R Kumar

引用次数: 2

9-(2-C-Cyano-2-deoxy-beta-D-arabino-pentofuranosyl)guanine, a potential antitumor agent against B-lymphoma infected with Kaposi's sarcoma-associated herpesvirus. 9-(2- c -氰-2-脱氧- β -d -阿拉伯-戊呋喃基)鸟嘌呤，一种潜在的抗卡波西肉瘤相关疱疹病毒感染b淋巴瘤的抗肿瘤药物。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp048

Satoshi Ichikawa, Masaki Otawa, Yasuhiro Teishikata, Koji Yamada, Masahiro Fujimuro, Hideyoshi Yokosawa, Akira Matsuda

引用次数: 2

Synthesis and properties of new fluorescent nucleosides and oligodeoxynucleotides derived from 5-formyl-2'-deoxyuridine. 5-甲酰基-2'-脱氧尿苷新荧光核苷和寡脱氧核苷酸的合成与性质

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp068

Wataru Hirose, Kousuke Sato, Akira Matsuda

引用次数: 3

Inhibition of influenza virus by baculovirus-mediated shRNA. 杆状病毒介导的shRNA对流感病毒的抑制作用

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp144

Hitoshi Suzuki, Hiroshi Saitoh, Tomoyuki Suzuki, Hiroshi Takaku

引用次数: 10

Investigation of the mechanism of the fluorescent emission of the silylated pyrene modified DNA. 硅烷化芘修饰DNA荧光发射机理的研究。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp016

Tomohisa Moriguchi, Masayoshi Hattori, Tohru Sekiguchi, Kazuo Shinozuka

引用次数: 2

Synthesis of exciton-controlled fluorescent probes for RNA imaging. 用于RNA成像的激子控制荧光探针的合成。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp078

Shuji Ikeda, Takeshi Kubota, Hiroyuki Yanagisawa, Mizue Yuki, Akimitsu Okamoto

引用次数: 3