Nucleic acids symposium series (2004)最新文献_第5页

9-(2-C-Cyano-2-deoxy-beta-D-arabino-pentofuranosyl)guanine, a potential antitumor agent against B-lymphoma infected with Kaposi's sarcoma-associated herpesvirus. 9-(2- c -氰-2-脱氧- β -d -阿拉伯-戊呋喃基)鸟嘌呤，一种潜在的抗卡波西肉瘤相关疱疹病毒感染b淋巴瘤的抗肿瘤药物。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp048

Satoshi Ichikawa, Masaki Otawa, Yasuhiro Teishikata, Koji Yamada, Masahiro Fujimuro, Hideyoshi Yokosawa, Akira Matsuda

引用次数: 2

Synthesis of 5'-fluoro-2'-beta-methylneplanocin analogues. 5'-氟-2'- β -甲基萘醌类似物的合成。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp051

Hiroki Kumamoto, Marina Kobayashi, Hiromichi Tanaka

引用次数: 1

Acridone-labeled DNA aptamer for the detection of biomolecules. 用于检测生物分子的吖啶酮标记DNA适体。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp128

Hiroaki Ozaki, Yasuhisa Hagiwara, Hiromi Asakura, Masayasu Kuwahara

引用次数: 1

Aptazyme-based biosensors using a eukaryotic cell-free translation system. 利用真核生物无细胞翻译系统的适体酶生物传感器。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp131

Atsushi Ogawa

{"title":"Aptazyme-based biosensors using a eukaryotic cell-free translation system.","authors":"Atsushi Ogawa","doi":"10.1093/nass/nrp131","DOIUrl":"https://doi.org/10.1093/nass/nrp131","url":null,"abstract":"I have constructed a novel aptazyme-based biosensor system for detecting cofactors of the aptazymes using a cell-free luciferase synthesis in wheat germ extract. In this system, the activity of the aptazyme that is fused to a 5'-untranslated region of a luciferase gene can be detected as luciferase expression. In translating the aptazyme-fused mRNA as-is using a wheat germ cell-free translation system, the luciferase is almost not expressed because of the following triple suppression effects: (1) 5'-terminal three bases and (2) 5'-terminal duplex prevent the ribosome from binding to own mRNA; (3) if the ribosome binds, translation of a mimic gene in the aptazyme inhibits that of the downstream luciferase gene (OFF state). In contrast, in the presence of the aptazyme cofactor, the aptazyme in mRNA is self-cleaved to produce an aptazyme-free luciferase gene, which is translated efficiently (ON state). The ON/OFF efficiency and the detection limit of the aptazyme-based biosensor for theophylline are much higher and lower, respectively, compared to those of previously-reported one that utilizes a prokaryotic translation system.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"261-2"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28397577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional DNA nanostructures constructed by folding of multiple rectangles. 通过折叠多个矩形构建三维DNA纳米结构。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp041

Masayuki Endo, Hiroshi Sugiyama

引用次数: 4

Photo-controllable aptamer. Photo-controllable适体。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp098

Shinzi Ogasawara, Mizuo Maeda

引用次数: 3

Synthesis and properties of new fluorescent nucleosides and oligodeoxynucleotides derived from 5-formyl-2'-deoxyuridine. 5-甲酰基-2'-脱氧尿苷新荧光核苷和寡脱氧核苷酸的合成与性质

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp068

Wataru Hirose, Kousuke Sato, Akira Matsuda

引用次数: 3

Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae. 酿酒酵母tRNA成熟各阶段修饰状态的精确分析。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp151

Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki

{"title":"Precise analysis of modification status at various stage of tRNA maturation in Saccharomyces cerevisiae.","authors":"Takayuki Ohira, Kenjyo Miyauchi, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki","doi":"10.1093/nass/nrp151","DOIUrl":"https://doi.org/10.1093/nass/nrp151","url":null,"abstract":"Transfer RNAs (tRNAs) are decorated with various post-transcriptional modifications which are enzymatically introduced at various stages of maturation. It is known that eukaryotic tRNAs are modified both in nucleus and cytoplasm. However, the order of tRNA modifications remains to be investigated. To unveil the precise timing of each modification associated with tRNA processing, we isolated precursor forms of Saccharomyces cerevisiae tRNAs at different stages of maturation, and analyzed their primary structures including modifications by mass spectrometry. The primary transcript of tRNA(Ile) was isolated from the tRNA fraction co-precipitated with Lhp1p, a yeast homolog of La protein. The precursor tRNA(Ile) without 3'-trailer sequence was isolated from the expression controllable strain when RNase P was transiently inactivated. Mass spectrometric analyses of these tRNAs revealed that many modifications were fully introduced at the stage of primary transcript, however, some modifications were partially introduced.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"301-2"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28475071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Investigation of the mechanism of the fluorescent emission of the silylated pyrene modified DNA. 硅烷化芘修饰DNA荧光发射机理的研究。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp016

Tomohisa Moriguchi, Masayoshi Hattori, Tohru Sekiguchi, Kazuo Shinozuka

引用次数: 2

Physiological role of RsgA in ribosome biosynthesis. RsgA在核糖体生物合成中的生理作用。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp154

Yoichi Hase, Shinichiro Yokoyama, Takatugu Kimura, Shimon Goto, Akira Muto, Hyouta Himeno

引用次数: 1