发布求助
文献互助 智能选刊 最新文献

Nucleic acids symposium series (2004)最新文献

筛选
英文 中文
Association of nuclear-intermediate filament lamin B1 with necrotic- and apoptotic-morphologies in cell death induced by 5-fluoro-2'-deoxyuridine. 在 5-氟-2'-脱氧尿苷诱导的细胞死亡过程中，核中间膜层粘连蛋白 B1 与细胞坏死和凋亡形态的相关性。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp147
Akira Sato, Akito Satake, Akiko Hiramoto, Akiko Okamatsu, Kentaro Nakama, Hye-Sook Kim, Yusuke Wataya
{"title":"Association of nuclear-intermediate filament lamin B1 with necrotic- and apoptotic-morphologies in cell death induced by 5-fluoro-2'-deoxyuridine.","authors":"Akira Sato, Akito Satake, Akiko Hiramoto, Akiko Okamatsu, Kentaro Nakama, Hye-Sook Kim, Yusuke Wataya","doi":"10.1093/nass/nrp147","DOIUrl":"10.1093/nass/nrp147","url":null,"abstract":"<p><p>We report that anticancer 5-fluoro-2'-deoxyuridine (FUdR) shows cytotoxicity against mouse cancer cell line FM3A cells, using a progeny clone F28-7 and its variant F28-7-A. In this process, the cell-death morphology is different between F28-7 and F28-7-A cells, that is, necrosis in F28-7 but apoptosis in F28-7-A cells. Recently we have investigated the gene and protein expression profiles of necrosis and apoptosis induced by FUdR using transcriptomic and proteomic analyses. In the proteomic analysis of these cells before their exposure to FUdR, the nuclear inner-membrane protein lamin B1 is up-regulated in F28-7 but not in F28-7-A, suggesting that lamin B1 may possess a function to regulate the morphology of cell-death. A knockdown of lamin B1 expression in F28-7 cells has now been performed by use of the small interfering RNA technique, resulting in a decrease of the lamin B1-expression level down to the level in F28-7-A. Remarkably, the FUdR-induced death morphology of this knocked-down F28-7 was apoptosis, definitely different from the necrosis that occurs in the FUdR-treated original F28-7. Our present finding provides an interesting possibility that lamin-B1 may have an important role in regulating cell-death morphology.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"293-4"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28470966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Efficient energy transfer from pyrene to perylene assembled inside DNA duplex. 有效的能量转移从芘到聚在DNA双链内的苝。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp015
Hiromu Kashida, Tomohiko Takatsu, Hiroyuki Asanuma
{"title":"Efficient energy transfer from pyrene to perylene assembled inside DNA duplex.","authors":"Hiromu Kashida,&nbsp;Tomohiko Takatsu,&nbsp;Hiroyuki Asanuma","doi":"10.1093/nass/nrp015","DOIUrl":"https://doi.org/10.1093/nass/nrp015","url":null,"abstract":"<p><p>Pyrene (donor) and perylene (acceptor) were assembled in a DNA duplex to increase the apparent Stokes' shift of perylene. Multiple donors were introduced in the vicinity of acceptors via D-threoninol and natural base pairs were inserted between donors and acceptors in order to suppress undesired interactions between them. When two pyrene moieties were located in proximity to one perylene with one base pair inserted between them, efficient FRET occurred within the duplex. Thus, strong emission at 495 nm was observed from perylene when excited at 345 nm where pyrene has its absorption. The apparent Stokes' shift became as large as 115 nm with a high FRET efficiency (Phi>1). However, the introduction of more than two pyrenes did not enhance the fluorescence intensity of perylene, due to the short Förster radius (R(0)) of the donor pyrene.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"29-30"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28475049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural basis for the specificity of thymidylate kinases from human pathogens: implications for nucleotide analogues activation. 人类病原体胸腺苷酸激酶特异性的结构基础:核苷酸类似物激活的意义。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp021
Philippe Meyer, Christophe Caillat, Dimitri Topalis, Jan Balzarini, Dominique Deville-Bonne
{"title":"Structural basis for the specificity of thymidylate kinases from human pathogens: implications for nucleotide analogues activation.","authors":"Philippe Meyer,&nbsp;Christophe Caillat,&nbsp;Dimitri Topalis,&nbsp;Jan Balzarini,&nbsp;Dominique Deville-Bonne","doi":"10.1093/nass/nrp021","DOIUrl":"https://doi.org/10.1093/nass/nrp021","url":null,"abstract":"<p><p>Several human pathogens possess nucleoside or nucleotide kinases with large substrate specificity compared to their human counterparts. This phenomenon has been successfully exploited for the specific targeting of prodrugs such as Acyclovir against herpes virus. Combined structural and biochemical studies of these enzymes can thus provide essential information for the rational design of specific antimicrobial agents. Here we studied the structural basis for the specificity of a thymidylate kinase from the poxvirus family. Poxvirus thymidylate kinase has unusual substrate specificity and can accept bulky analogues such as 5-bromo-vinyl-dUMP (BVdUMP). The 2 A crystal structure of the thymidylate kinase bound to this compound now gives the structural basis for its specific molecular recognition.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"41"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28475055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Synthetic nanocircular RNA for controlling of gene expression. 合成纳米环状RNA控制基因表达。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp033
Hiroshi Abe, Naoko Abe, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito
{"title":"Synthetic nanocircular RNA for controlling of gene expression.","authors":"Hiroshi Abe,&nbsp;Naoko Abe,&nbsp;Miwako Uda,&nbsp;Satoshi Tsuneda,&nbsp;Yoshihiro Ito","doi":"10.1093/nass/nrp033","DOIUrl":"https://doi.org/10.1093/nass/nrp033","url":null,"abstract":"<p><p>We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"65-6"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28398376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Acceleration of guanine oxidation under visible light irradiation by photon upconversion based on triplet-triplet annihilation. 基于三重态-三重态湮灭的光子上转换加速可见光照射下鸟嘌呤氧化。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp092
Kazuo Tanaka, Narufumi Kitamura, Kenichi Inafuku, Yoshiki Chujo
{"title":"Acceleration of guanine oxidation under visible light irradiation by photon upconversion based on triplet-triplet annihilation.","authors":"Kazuo Tanaka,&nbsp;Narufumi Kitamura,&nbsp;Kenichi Inafuku,&nbsp;Yoshiki Chujo","doi":"10.1093/nass/nrp092","DOIUrl":"https://doi.org/10.1093/nass/nrp092","url":null,"abstract":"<p><p>We report the fluorescent polymer complex which can show fluorescence emission at 380 nm with the excitation of 520 nm in aqueous media. This photon upconversion based on triplet-triplet annihilation can efficiently take place via inter-molecular energy transfers between the Ru complex as a sensitizer and anthracene molecules as an emitter captured into the water-soluble network polymers. We performed the oxidation reaction of 2'-deoxyguanosine by riboflavin in the presence of the polymer complex with the visible light irradiation. It was clearly indicated that oxidative decomposition can be accelerated by UV light generation via upconversion based on triplet-triplet annihilation.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"183-4"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28398385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Development of novel chemical probes to detect abasic sites in DNA. 新型化学探针检测DNA碱基位点的发展。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp023
Naoshi Kojima, Toshie Takebayashi, Akiko Mikami, Eiko Ohtsuka, Yasuo Komatsu
{"title":"Development of novel chemical probes to detect abasic sites in DNA.","authors":"Naoshi Kojima,&nbsp;Toshie Takebayashi,&nbsp;Akiko Mikami,&nbsp;Eiko Ohtsuka,&nbsp;Yasuo Komatsu","doi":"10.1093/nass/nrp023","DOIUrl":"https://doi.org/10.1093/nass/nrp023","url":null,"abstract":"<p><p>We chemically synthesized a series of aminooxy derivatives to develop novel probes for sensitive detection of abasic (AP) sites in DNA. The results of the conjugation reactions showed that the probes could efficiently react to AP sites by introducing an aromatic or a guanidino group in their structures. In particular, the probe having both functional groups showed the most effective reactivity, indicating that hydrophobic and electrostatic interactions cooperatively acted in the reaction of the probe to AP sites. We then synthesized a biotinylated probe and succeeded in more sensitive detection of AP sites in genomic DNA than with the conventional aldehyde reactive probe (ARP).</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"45-6"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28396106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Development of peptide-oligonucleotide conjugates for regulation of small RNA function. 肽-寡核苷酸偶联物调控小RNA功能的研究进展。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp027
Asako Yamayoshi, Daiki Momokawa, Akio Kobori, Akira Murakami
{"title":"Development of peptide-oligonucleotide conjugates for regulation of small RNA function.","authors":"Asako Yamayoshi,&nbsp;Daiki Momokawa,&nbsp;Akio Kobori,&nbsp;Akira Murakami","doi":"10.1093/nass/nrp027","DOIUrl":"https://doi.org/10.1093/nass/nrp027","url":null,"abstract":"<p><p>Recently, various microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs) have been identified and play important roles in gene regulatory networks. However it is a little known about their biological functions. These small RNAs exhibit their function to form a ribonucleoprotein complex, the RISC (RNA-induced silencing complex), which modulates gene expression by translational repression. In this study, we developed a novel peptide antagonist to inhibit the RISC function. The peptide was conjugated to 2'-O-methyl oligoribonucleotides, which have a complementary sequence to the guide strand of siRNA, and regulatory effects of the peptide on RISC activity were examined. It was revealed that the peptide drastically enhanced inhibitory effects of the oligonucleotide on RISC activity. Here we demonstrate our peptide-oligonucleotide conjugate that can provide a powerful and specific way to regulate the small RNA function.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"53-4"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28396110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins. 高耐热性RNA发夹的紫外熔化分析的精确曲线拟合程序。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp114
Junji Kawakami, Yuko Tanaka, Kae Kishimoto
{"title":"Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins.","authors":"Junji Kawakami,&nbsp;Yuko Tanaka,&nbsp;Kae Kishimoto","doi":"10.1093/nass/nrp114","DOIUrl":"https://doi.org/10.1093/nass/nrp114","url":null,"abstract":"<p><p>Thermostable RNA hairpins with specific loop sequences are critically important structural elements for proper folding of functional RNAs. Thermodynamic parameters of the structural elements are indispensable for secondary structure prediction. However when RNA folds into highly thermostable hairpin-loop structures, it is difficult to determine the melting temperature and other thermodynamic parameters from the normal UV melting analysis. In this study, we demonstrate a procedure to solve the problem using our software, Jfit. UV absorbance in the higher temperature region above 95 degrees C and the upper baseline were estimated using this analytical procedure. The ideal lines of -RlnK vs. 1/T were generated from the predicted thermodynamic parameters and the solution candidates were compared with the van't Hoff plot generated from the measured melting curve to judge their self-consistency. As a result, our analysis was able to reduce the number of the possible solution and to specify the best set of thermodynamic parameters for the stable hairpin formation.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"227-8"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28396477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Synthesis of 5'-fluoro-2'-beta-methylneplanocin analogues. 5'-氟-2'- β -甲基萘醌类似物的合成。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp051
Hiroki Kumamoto, Marina Kobayashi, Hiromichi Tanaka
{"title":"Synthesis of 5'-fluoro-2'-beta-methylneplanocin analogues.","authors":"Hiroki Kumamoto,&nbsp;Marina Kobayashi,&nbsp;Hiromichi Tanaka","doi":"10.1093/nass/nrp051","DOIUrl":"https://doi.org/10.1093/nass/nrp051","url":null,"abstract":"<p><p>Synthesis of 5'-fluoro-2'-beta-methylneplanocin analogues (5) was carried out. The cyclopentenone 16 was prepared from methyl mannopyranoside by using ring closing metathesis, stereoselective introduction of methyl group, and seleno cyclization as representative steps. Introduction of a fluorine atom was conducted by electrophilic fluorination. Antiviral activity of 5 will also be presented.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"101-2"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28397568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Acridone-labeled DNA aptamer for the detection of biomolecules. 用于检测生物分子的吖啶酮标记DNA适体。
Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp128
Hiroaki Ozaki, Yasuhisa Hagiwara, Hiromi Asakura, Masayasu Kuwahara
{"title":"Acridone-labeled DNA aptamer for the detection of biomolecules.","authors":"Hiroaki Ozaki,&nbsp;Yasuhisa Hagiwara,&nbsp;Hiromi Asakura,&nbsp;Masayasu Kuwahara","doi":"10.1093/nass/nrp128","DOIUrl":"https://doi.org/10.1093/nass/nrp128","url":null,"abstract":"<p><p>An acridone-labeled DNA aptamer was synthesized to produce an aptamer probe. This aptamer probe could detect a specific molecule based on conformational changes upon binding of the specific molecules and the quenching of acridone emission.</p>","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"255-6"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28397574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信