新型化学探针检测DNA碱基位点的发展。

Naoshi Kojima, Toshie Takebayashi, Akiko Mikami, Eiko Ohtsuka, Yasuo Komatsu
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引用次数: 1

摘要

我们化学合成了一系列氨基衍生物,以开发新的探针,用于灵敏检测DNA中的碱基(AP)位点。共轭反应结果表明,探针可以通过在其结构中引入芳香族或胍基有效地与AP位点发生反应。特别是,具有两个官能团的探针表现出最有效的反应活性,这表明疏水和静电相互作用共同作用于探针对AP位点的反应。然后,我们合成了一种生物素化探针,并成功地比传统的醛反应探针(ARP)更敏感地检测基因组DNA中的AP位点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of novel chemical probes to detect abasic sites in DNA.

We chemically synthesized a series of aminooxy derivatives to develop novel probes for sensitive detection of abasic (AP) sites in DNA. The results of the conjugation reactions showed that the probes could efficiently react to AP sites by introducing an aromatic or a guanidino group in their structures. In particular, the probe having both functional groups showed the most effective reactivity, indicating that hydrophobic and electrostatic interactions cooperatively acted in the reaction of the probe to AP sites. We then synthesized a biotinylated probe and succeeded in more sensitive detection of AP sites in genomic DNA than with the conventional aldehyde reactive probe (ARP).

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