合成纳米环状RNA控制基因表达。

Hiroshi Abe, Naoko Abe, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito
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引用次数: 4

摘要

我们设计并合成了用于RNA干扰的哑铃状纳米环状RNA,它由一个茎和两个环组成(1)。RNA哑铃被人类Dicer酶特异性识别和切割,并因此在细胞中转化为双链RNA,尽管这种RNA在血清中不易降解。对其结构进行了优化,使其RNAi活性最大化。当茎长为23个碱基对时,活性最强。与正常的siRNA相比,RNAi的活性被分子的形状延长了,这是一个无穷无尽的结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synthetic nanocircular RNA for controlling of gene expression.

We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.

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