Development of peptide-oligonucleotide conjugates for regulation of small RNA function.

Asako Yamayoshi, Daiki Momokawa, Akio Kobori, Akira Murakami
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引用次数: 1

Abstract

Recently, various microRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs) have been identified and play important roles in gene regulatory networks. However it is a little known about their biological functions. These small RNAs exhibit their function to form a ribonucleoprotein complex, the RISC (RNA-induced silencing complex), which modulates gene expression by translational repression. In this study, we developed a novel peptide antagonist to inhibit the RISC function. The peptide was conjugated to 2'-O-methyl oligoribonucleotides, which have a complementary sequence to the guide strand of siRNA, and regulatory effects of the peptide on RISC activity were examined. It was revealed that the peptide drastically enhanced inhibitory effects of the oligonucleotide on RISC activity. Here we demonstrate our peptide-oligonucleotide conjugate that can provide a powerful and specific way to regulate the small RNA function.

肽-寡核苷酸偶联物调控小RNA功能的研究进展。
近年来,各种microRNAs (miRNAs)和内源性小干扰rna (sirna)被发现并在基因调控网络中发挥重要作用。然而,人们对它们的生物学功能知之甚少。这些小rna表现出形成核糖核蛋白复合物的功能,即rna诱导沉默复合物,通过翻译抑制调节基因表达。在这项研究中,我们开发了一种新的肽拮抗剂来抑制RISC功能。将该肽偶联到与siRNA引导链具有互补序列的2'- o -甲基寡核苷酸上,并检测了该肽对RISC活性的调节作用。结果表明,该肽显著增强了寡核苷酸对RISC活性的抑制作用。在这里,我们展示了我们的肽寡核苷酸缀合物,可以提供一个强大的和特定的方式来调节小RNA的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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