Production of yeast (m2G10) methyltransferase (Trm11 and Trm112 complex) in a wheat germ cell-free translation system.

Kazuki Okada, Yuki Muneyoshi, Yaeta Endo, Hiroyuki Hori
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引用次数: 13

Abstract

Transfer RNA (guanine-N(2)-)-methyltransferase [tRNA (m(2)G10) methyltransferase] catalyzes a methyl-transfer from S-adenosyl-L-methionine to N(2)-atom of guanine at position 10 (G10) in tRNA and generates N(2)-methylguanine at position 10 (m(2)G10). Yeast enzyme contains two protein subunits (Trm11 and Trm112). Trm11 protein is expected to be a catalytic subunit and Trm112 contains a Zinc-finger. In yeast cells, Trm112 binds not only to Trm11 but also to other proteins such as Lys9, Trm9, and Mtq2. Therefore, the Trm112 protein may regulate population of several protein complexes. To address these issues, we started the study on synthesis of Trm112 related protein complexes. In this meeting, we report synthesis of active Trm11-Trm112 complex in a wheat germ cell-free translation system.

小麦无生殖细胞翻译系统中酵母(m2G10)甲基转移酶(Trm11和Trm112复合体)的产生
转移RNA(鸟嘌呤-N(2)-)-甲基转移酶[tRNA (m(2)G10)甲基转移酶]催化s -腺苷- l-蛋氨酸甲基转移到tRNA中10 (G10)位置的鸟嘌呤N(2)-原子上,并在10 (m(2)G10)位置生成N(2)-甲基鸟嘌呤。酵母酶含有两个蛋白质亚基(Trm11和Trm112)。Trm11蛋白有望成为催化亚基,而Trm112含有锌指。在酵母细胞中,Trm112不仅与Trm11结合,还与其他蛋白质如Lys9、Trm9和Mtq2结合。因此,Trm112蛋白可能调控多种蛋白复合物的种群。针对这些问题,我们开始了Trm112相关蛋白复合物的合成研究。在本次会议上,我们报道了在小麦无生殖细胞翻译系统中合成活性Trm11-Trm112复合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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