Bacteriophage最新文献_第6页

Bacteriophage behavioral ecology: How phages alter their bacterial host's habits. 噬菌体行为生态学：噬菌体如何改变细菌宿主的习性？

Bacteriophage Pub Date : 2014-07-08 eCollection Date: 2014-01-01 DOI: 10.4161/bact.29866

Katherine R Hargreaves, Andrew M Kropinski, Martha Rj Clokie

引用次数: 0

Molecular basis of RNA polymerase promoter specificity switch revealed through studies of Thermus bacteriophage transcription regulator. 热噬菌体转录调节剂研究揭示RNA聚合酶启动子特异性开关的分子基础。

Bacteriophage Pub Date : 2014-05-29 eCollection Date: 2014-01-01 DOI: 10.4161/bact.29399

Konstantin Severinov, Leonid Minakhin, Shun-Ichi Sekine, Anna Lopatina, Shigeyuki Yokoyama

引用次数: 2

Rapid Burkholderia pseudomallei identification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS. 利用噬菌体扩增和MALDI-TOF质谱法快速鉴定假马利氏伯克氏菌及其耐药性。

Bacteriophage Pub Date : 2014-04-25 eCollection Date: 2014-01-01 DOI: 10.4161/bact.29011

Christopher R Cox, Nicholas R Saichek, Herbert P Schweizer, Kent J Voorhees

{"title":"Rapid Burkholderia pseudomallei identification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS.","authors":"Christopher R Cox, Nicholas R Saichek, Herbert P Schweizer, Kent J Voorhees","doi":"10.4161/bact.29011","DOIUrl":"https://doi.org/10.4161/bact.29011","url":null,"abstract":"Phage amplification detected by MALDI-TOF MS was investigated for rapid and simultaneous Burkholderia pseudomallei identification and ceftazidime resistance determination. B. pseudomallei ceftazidime susceptible and resistant ΔpurM mutant strains Bp82 and Bp82.3 were infected with broadly targeting B. pseudomallei phage ϕX216 and production of the m/z 37.6 kDa phage capsid protein observed by MALDI-TOF MS over the course of 3 h infections. This allowed for repoducible phage-based bacterial ID within 2 h of the onset of infection. MALDI-TOF MS-measured time to detection correlated with in silico modeling, which predicted an approximate 2 h detection time. Ceftazidime susceptible strain Bp82, while detectable in the absence of the drug, owing to the reliance of phage amplification on a viable host, was not detectable when 10 μg/mL ceftazidime was added at the onset of infection. In contrast, resistant strain Bp82.3 was detected in the same 2 h timeframe both with and without the addition of ceftazidime.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"4 ","pages":"e29011"},"PeriodicalIF":0.0,"publicationDate":"2014-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.29011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32524328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Morphotypes of virus-like particles in two hydrothermal vent fields on the East Scotia Ridge, Antarctica. 南极洲东斯科舍脊两个热液喷口中病毒样颗粒的形态型。

Bacteriophage Pub Date : 2014-04-02 eCollection Date: 2014-01-01 DOI: 10.4161/bact.28732

Andrew D Millard, Ian Hands-Portman, Katrin Zwirglmaier

引用次数: 7

Flap endonuclease of bacteriophage T7: Possible roles in RNA primer removal, recombination and host DNA breakdown. 噬菌体T7的皮瓣内切酶:在RNA引物去除、重组和宿主DNA分解中的可能作用。

Bacteriophage Pub Date : 2014-03-11 eCollection Date: 2014-01-01 DOI: 10.4161/bact.28507

Hitoshi Mitsunobu, Bin Zhu, Seung-Joo Lee, Stanley Tabor, Charles C Richardson

引用次数: 6

Genome analysis of the staphylococcal temperate phage DW2 and functional studies on the endolysin and tail hydrolase. 葡萄球菌温带噬菌体DW2的基因组分析及内溶素和尾水解酶的功能研究。

Bacteriophage Pub Date : 2014-03-06 eCollection Date: 2014-01-01 DOI: 10.4161/bact.28451

Ruth Keary, Olivia McAuliffe, R Paul Ross, Colin Hill, Jim O'Mahony, Aidan Coffey

{"title":"Genome analysis of the staphylococcal temperate phage DW2 and functional studies on the endolysin and tail hydrolase.","authors":"Ruth Keary, Olivia McAuliffe, R Paul Ross, Colin Hill, Jim O'Mahony, Aidan Coffey","doi":"10.4161/bact.28451","DOIUrl":"https://doi.org/10.4161/bact.28451","url":null,"abstract":"This study describes the genome of temperate Siphoviridae phage DW2, which is routinely propagated on Staphylococcus aureus DPC5246. The 41941 bp genome revealed an open reading frame (ORF1) which has a high level of homology with members of the resolvase subfamily of site-specific serine recombinase, involved in chromosomal integration and excision. In contrast, the majority of staphylococcal phages reported to date encode tyrosine recombinases. Two putative genes encoded by phage DW2 (ORF15 and ORF24) were highly homologous to the NWMN0273 and NWMN0280 genes encoding virulence factors carried on the genome of ϕNM4, a prophage in the genome of S. aureus Newman. Phage DW2 also encodes proteins highly homologous to two well-characterized Staphylococcus aureus pathogenicity island derepressors encoded by the staphylococcal helper phage 80α indicating that it may similarly act as a helper phage for mobility of pathogenicity islands in S. aureus. This study also focused on the enzybiotic potential of phage DW2. The structure of the putative endolysin and tail hydrolase were investigated and used as the basis for a cloning strategy to create recombinant peptidoglycan hydrolyzing proteins. After overexpression in E. coli, four of these proteins (LysDW2, THDW2, CHAPE1-153, and CHAPE1-163) were demonstrated to have hydrolytic activity against peptidoglycan of S. aureus and thus represent novel candidates for exploitation as enzybiotics.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"4 ","pages":"e28451"},"PeriodicalIF":0.0,"publicationDate":"2014-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.28451","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32570259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Life in Science: William C Summers. 科学生活:威廉·C·萨默斯。

Bacteriophage Pub Date : 2014-01-01 DOI: 10.4161/bact.26691

William C Summers

引用次数: 0

Molecular imaging of T4 phage in mammalian tissues and cells. 哺乳动物组织和细胞中T4噬菌体的分子成像。

Bacteriophage Pub Date : 2014-01-01 Epub Date: 2014-02-27 DOI: 10.4161/bact.28364

Zuzanna Kaźmierczak, Agnieszka Piotrowicz, Barbara Owczarek, Katarzyna Hodyra, Paulina Miernikiewicz, Dorota Lecion, Marek Harhala, Andrzej Górski, Krystyna Dąbrowska

引用次数: 33

Application of bacteriophages for detection of foodborne pathogens. 应用噬菌体检测食源性病原体。

Bacteriophage Pub Date : 2014-01-01 Epub Date: 2014-02-07 DOI: 10.4161/bact.28137

Mathias Schmelcher, Martin J Loessner

引用次数: 0

Different approaches for using bacteriophages against antibiotic-resistant bacteria. 利用噬菌体对抗耐药细菌的不同方法。

Bacteriophage Pub Date : 2014-01-01 Epub Date: 2014-03-11 DOI: 10.4161/bact.28491

Ido Yosef, Ruth Kiro, Shahar Molshanski-Mor, Rotem Edgar, Udi Qimron

{"title":"Different approaches for using bacteriophages against antibiotic-resistant bacteria.","authors":"Ido Yosef, Ruth Kiro, Shahar Molshanski-Mor, Rotem Edgar, Udi Qimron","doi":"10.4161/bact.28491","DOIUrl":"https://doi.org/10.4161/bact.28491","url":null,"abstract":"Bacterial resistance to antibiotics is an emerging threat requiring urgent solutions. Ever since their discovery, lytic bacteriophages have been suggested as therapeutic agents, but their application faces various obstacles: sequestration of the phage by the spleen and liver, antibodies against the phage, narrow host range, poor accessibility to the infected tissue, and bacterial resistance. Variations on bacteriophage use have been suggested, such as temperate phages as gene-delivery vehicles into pathogens. This approach, which is proposed to sensitize pathogens residing on hospital surfaces and medical personnel's skin, and its prospects are described in this addendum. Furthermore, phage-encoded products have been proposed as weapons against antibiotic resistance in bacteria. We describe a new phage protein which was identified during basic research into T7 bacteriophages. This protein may serendipitously prove useful for treating antibiotic-resistant pathogens. We believe that further basic research will lead to novel strategies in the fight against antibiotic-resistant bacteria.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"4 1","pages":"e28491"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.28491","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32195796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23