利用噬菌体扩增和MALDI-TOF质谱法快速鉴定假马利氏伯克氏菌及其耐药性。

Bacteriophage Pub Date : 2014-04-25 eCollection Date: 2014-01-01 DOI:10.4161/bact.29011
Christopher R Cox, Nicholas R Saichek, Herbert P Schweizer, Kent J Voorhees
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引用次数: 12

摘要

采用MALDI-TOF质谱法对噬菌体扩增进行快速、同步鉴定假马氏伯克氏菌和头孢他啶耐药性测定。假假杆菌头孢他噻肟敏感和耐药ΔpurM突变株Bp82和Bp82.3被广泛靶向假假杆菌噬菌体大肠杆菌感染,在感染3小时后,用MALDI-TOF MS观察到m/z 37.6 kDa噬菌体衣壳蛋白的产生。这允许在感染开始后2小时内可复制的基于噬菌体的细菌ID。MALDI-TOF ms测量到检测的时间与硅模型相关,预测了大约2小时的检测时间。头孢他啶敏感菌株Bp82在不加头孢他啶的情况下,由于噬菌体对活菌的扩增依赖,在感染开始时加入10 μg/mL头孢他啶时,未检出。相比之下,在添加和未添加头孢他啶的情况下,在相同的2 h时间内检测到耐药菌株Bp82.3。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Rapid <i>Burkholderia pseudomallei</i> identification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS.

Rapid <i>Burkholderia pseudomallei</i> identification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS.

Rapid <i>Burkholderia pseudomallei</i> identification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS.

Rapid Burkholderia pseudomallei identification and antibiotic resistance determination by bacteriophage amplification and MALDI-TOF MS.

Phage amplification detected by MALDI-TOF MS was investigated for rapid and simultaneous Burkholderia pseudomallei identification and ceftazidime resistance determination. B. pseudomallei ceftazidime susceptible and resistant ΔpurM mutant strains Bp82 and Bp82.3 were infected with broadly targeting B. pseudomallei phage ϕX216 and production of the m/z 37.6 kDa phage capsid protein observed by MALDI-TOF MS over the course of 3 h infections. This allowed for repoducible phage-based bacterial ID within 2 h of the onset of infection. MALDI-TOF MS-measured time to detection correlated with in silico modeling, which predicted an approximate 2 h detection time. Ceftazidime susceptible strain Bp82, while detectable in the absence of the drug, owing to the reliance of phage amplification on a viable host, was not detectable when 10 μg/mL ceftazidime was added at the onset of infection. In contrast, resistant strain Bp82.3 was detected in the same 2 h timeframe both with and without the addition of ceftazidime.

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