Bacteriophage最新文献_第2页

The impact of orally administered phages on host immune response and surrounding microbial communities 口服噬菌体对宿主免疫反应和周围微生物群落的影响

Bacteriophage Pub Date : 2016-07-02 DOI: 10.1080/21597081.2016.1211066

Yingying Hong, J. Thimmapuram, Jiayi Zhang, Clayton K. Collings, K. Bhide, Kyle Schmidt, P. Ebner

{"title":"The impact of orally administered phages on host immune response and surrounding microbial communities","authors":"Yingying Hong, J. Thimmapuram, Jiayi Zhang, Clayton K. Collings, K. Bhide, Kyle Schmidt, P. Ebner","doi":"10.1080/21597081.2016.1211066","DOIUrl":"https://doi.org/10.1080/21597081.2016.1211066","url":null,"abstract":"ABSTRACT Numerous studies have shown the efficacy of phage therapy in reducing foodborne pathogen carriage in food animals. Fewer studies have focused on host reactions, especially in terms of phage-mediated acute immune responses and effects on the gut microbiome. Here we administered E. coli O157:H7 phages in low (single dose of 105 PFU) or high (single dose of 107 PFU) quantities to mice. While there were time points at which cytokine levels in different treatment groups differed from one another, all cytokine levels remained within normal ranges for mice regardless of treatment. Similarly, the patterns of these differences were not dose related, indicating that phage treatment did not result in a strong acute immune response as measured here. In separate experiments, 3-week-old pigs received a diet containing an in-feed antibiotic or daily phage treatment. After two weeks, microbial DNA of ileal, cecal, and fecal contents was characterized using 16S rRNA sequencing. There were no statistical differences in performance among the different groups. Compared to control pigs (no antibiotic, no phage), antibiotic treatment significantly altered ileal microbiome composition (P < 0.05), with Bacilli being most affected (antibiotic treated: 22%; control: 76%; FDR = 0.0572). No significant differences were observed in cecal and fecal microbiome composition between antibiotic-treated and control pigs, and there were no differences in gut microbiome composition between phage treated and control pigs in any intestinal compartment. Significant abundance differences were observed at the OTU level, with OTUs belonging to genera such as Lactobacillus and Streptococcus being over- or under-represented in either antibiotic or phage treated groups compared to control pigs. Determining whether these changes are deleterious to host, however, requires further study.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80611148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Grete Kellenberger-Gujer: Molecular biology research pioneer. Grete Kellenberger-Gujer:分子生物学研究先驱。

Bacteriophage Pub Date : 2016-04-05 eCollection Date: 2016-04-01 DOI: 10.1080/21597081.2016.1173168

Sandra Citi, Douglas E Berg

引用次数: 1

Counteracting selection for antibiotic-resistant bacteria. 对耐抗生素细菌的对抗选择。

Bacteriophage Pub Date : 2016-03-07 eCollection Date: 2016-01-01 DOI: 10.1080/21597081.2015.1096996

Ido Yosef, Miriam Manor, Udi Qimron

引用次数: 13

Bacteriophage P2. 噬菌体P2。

Bacteriophage Pub Date : 2016-02-18 eCollection Date: 2016-01-01 DOI: 10.1080/21597081.2016.1145782

Gail E Christie, Richard Calendar

引用次数: 35

T7 ejectosome assembly: A story unfolds. T7弹射体组装:一个故事展开。

Bacteriophage Pub Date : 2016-02-18 eCollection Date: 2016-01-01 DOI: 10.1080/21597081.2015.1128513

Sebastian Leptihn, Julia Gottschalk, Andreas Kuhn

引用次数: 20

The diverse genetic switch of enterobacterial and marine telomere phages. 肠杆菌和海洋端粒噬菌体的多样性遗传开关。

Bacteriophage Pub Date : 2016-02-18 eCollection Date: 2016-04-01 DOI: 10.1080/21597081.2016.1148805

Jens A Hammerl, Claudia Jäckel, Eugenia Funk, Sabrina Pinnau, Christin Mache, Stefan Hertwig

{"title":"The diverse genetic switch of enterobacterial and marine telomere phages.","authors":"Jens A Hammerl, Claudia Jäckel, Eugenia Funk, Sabrina Pinnau, Christin Mache, Stefan Hertwig","doi":"10.1080/21597081.2016.1148805","DOIUrl":"https://doi.org/10.1080/21597081.2016.1148805","url":null,"abstract":"Temperate bacteriophages possess a genetic switch which regulates the lytic and lysogenic cycle. The genomes of the enterobacterial telomere phages N15, PY54 and ϕKO2 harbor a primary immunity region (immB) comprising genes for the prophage repressor, the lytic repressor and a putative antiterminator, similar to CI, Cro and Q of lambda, respectively. Moreover, N15 and ϕKO2 contain 3 related operator (OR) sites between cI and cro, while only one site (OR3) has been detected in PY54. Marine telomere phages possess a putative cI gene but not a cro-like gene. Instead, a gene is located at the position of cro, whose product shows some similarity to the PY54 ORF42 product, the function of which is unknown. We have determined the transcription start sites of the predicted repressor genes of N15, PY54, ϕKO2 and of the marine telomere phage VP58.5. The influence of the genes on phage propagation was analyzed in E. coli, Y. enterocolitica and V.parahaemolyticus. We show that the repressors and antiterminators of N15, ϕKO2 and PY54 exerted their predicted activities. However, while the proteins of both N15 and ϕKO2 affected lysis and lysogeny by N15, they did not affect PY54 propagation. On the other hand, the respective PY54 proteins exclusively influenced the propagation of this phage. The immB region of VP58.5 contains 2 genes that revealed prophage repressor activity, while a lytic repressor gene could not be identified. The results indicate an unexpected diversity of the growth regulation mechanisms in these temperate phages. ","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"6 2","pages":"e1148805"},"PeriodicalIF":0.0,"publicationDate":"2016-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21597081.2016.1148805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34427454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

CTXϕ: Exploring new alternatives in host factor-mediated filamentous phage replications. ctxφ:探索宿主因子介导的丝状噬菌体复制的新选择。

Bacteriophage Pub Date : 2016-02-11 eCollection Date: 2016-04-01 DOI: 10.1080/21597081.2015.1128512

Eriel Martínez, Javier Campos-Gómez, François-Xavier Barre

引用次数: 5

Lactococcus lactis phage TP901-1 as a model for Siphoviridae virion assembly. 乳球菌噬菌体TP901-1作为虹膜病毒科病毒粒子组装的模型。

Bacteriophage Pub Date : 2016-01-06 eCollection Date: 2016-01-01 DOI: 10.1080/21597081.2015.1123795

Jennifer Mahony, Stephen R Stockdale, Barry Collins, Silvia Spinelli, Francois P Douillard, Christian Cambillau, Douwe van Sinderen

{"title":"Lactococcus lactis phage TP901-1 as a model for Siphoviridae virion assembly.","authors":"Jennifer Mahony, Stephen R Stockdale, Barry Collins, Silvia Spinelli, Francois P Douillard, Christian Cambillau, Douwe van Sinderen","doi":"10.1080/21597081.2015.1123795","DOIUrl":"https://doi.org/10.1080/21597081.2015.1123795","url":null,"abstract":"Phages infecting Lactococcus lactis pose a serious threat to the dairy fermentation sector. Consequently, they are among the most thoroughly characterized Gram positive-infecting phages. The majority of lactococcal phages belong to the tailed family of phages named the Siphoviridae. The coliphage lambda and the Bacillus subtilis phage SPP1 have been the predominant comparators for emerging siphophages both genomically and structurally and both phages recognize a membrane protein receptor. In contrast, the lactococcal P335 group phage TP901-1 attaches to cell wall surface polysaccharides. It is a typical \"lambdoid\" siphophage possessing a long non-contractile tail and a genomic architecture reminiscent of lambda and SPP1 despite low or undetectable sequence homology in many of its encoded products, especially those involved in host recognition. A functional analysis of the structural components of TP901-1 was undertaken based on the characterization of a series of mutants in the region encoding the capsid and tail morphogenetic elements. Through this analysis, it was possible to deduce that, despite the lack of sequence homology, the overall genomic architecture of Siphoviridae phages typified by functional synteny is conserved. Furthermore, a model of the TP901-1 assembly pathway was developed with potential implications for many tailed phages.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":" ","pages":"e1123795"},"PeriodicalIF":0.0,"publicationDate":"2016-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21597081.2015.1123795","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34358012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease. 蜜蜂(Apis mellifera)感染美国褐变病病原类芽孢杆菌(Paenibacillus)幼虫的实验噬菌体治疗。

Bacteriophage Pub Date : 2016-01-05 eCollection Date: 2016-01-01 DOI: 10.1080/21597081.2015.1122698

Diane G Yost, Philippos Tsourkas, Penny S Amy

{"title":"Experimental bacteriophage treatment of honeybees (Apis mellifera) infected with Paenibacillus larvae, the causative agent of American Foulbrood Disease.","authors":"Diane G Yost, Philippos Tsourkas, Penny S Amy","doi":"10.1080/21597081.2015.1122698","DOIUrl":"https://doi.org/10.1080/21597081.2015.1122698","url":null,"abstract":"American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":" ","pages":"e1122698"},"PeriodicalIF":0.0,"publicationDate":"2016-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21597081.2015.1122698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34358011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Development of expanded host range phage active on biofilms of multi-drug resistant Pseudomonas aeruginosa. 多重耐药铜绿假单胞菌生物膜活性扩展宿主范围噬菌体的研究进展。

Bacteriophage Pub Date : 2016-01-05 eCollection Date: 2016-01-01 DOI: 10.1080/21597081.2015.1096995

Abigail C Mapes, Barbara W Trautner, Kershena S Liao, Robert F Ramig

{"title":"Development of expanded host range phage active on biofilms of multi-drug resistant Pseudomonas aeruginosa.","authors":"Abigail C Mapes, Barbara W Trautner, Kershena S Liao, Robert F Ramig","doi":"10.1080/21597081.2015.1096995","DOIUrl":"10.1080/21597081.2015.1096995","url":null,"abstract":"Phage therapy is a promising treatment of multi-drug resistant (MDR) bacterial infections but is limited by the narrow host range of phage. To overcome this limitation, we developed a host range expansion (HRE) protocol that expands the host range of Pseudomonas aeruginosa-specific phage by cycles of co-incubation of phage with multiple P. aeruginosa strains. Application of the HRE protocol to a mixture of 4 phages, using 16 P. aeruginosa strains for development, resulted in undefined phage mixtures with greatly expanded host range. Individual phage clones derived from the undefined mixture had expanded host ranges but no individual clone could lyse all of the strains covered by the undefined mixture from which it was isolated. Reconstituting host range-characterized clones into cocktails produced defined cocktails with predictable and broad host ranges. The undefined mixture from the 30th cycle of the mixed-phage HRE (4ϕC30) showed a dose-dependent ability to prevent biofilm formation by, and to reduce a pre-existing biofilm of, 3 P. aeruginosa clinical isolates that produced high amounts of biofilm. A defined cocktail reconstituted from 3 host range-characterized clones had activity on high biofilm-formers susceptible to the phage. Phage therapy was superior to antibiotic therapy (levofloxacin) in a strain of P. aeruginosa that was resistant to levofloxacin. The HRE protocol establishes a rapid approach to create libraries of phage clones and phage cocktails with broad host range, defined composition and anti-biofilm activity.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":" ","pages":"e1096995"},"PeriodicalIF":0.0,"publicationDate":"2016-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21597081.2015.1096995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34358009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 62