肠杆菌和海洋端粒噬菌体的多样性遗传开关。

Bacteriophage Pub Date : 2016-02-18 eCollection Date: 2016-04-01 DOI:10.1080/21597081.2016.1148805
Jens A Hammerl, Claudia Jäckel, Eugenia Funk, Sabrina Pinnau, Christin Mache, Stefan Hertwig
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引用次数: 7

摘要

温带噬菌体具有调节裂解和溶原循环的遗传开关。肠杆菌端粒噬菌体N15、PY54和ko2的基因组含有一个初级免疫区(imb),由噬菌体抑制因子、裂解抑制因子和推测的抗菌素基因组成,分别类似于λ的CI、Cro和Q。此外,N15和ko2在cI和cro之间含有3个相关的算子(OR)位点,而在PY54中只检测到一个位点(OR3)。海洋端粒噬菌体具有假定的cI基因,但不具有克隆样基因。相反,一个位于cro位置的基因,其产物与PY54 ORF42产物有一定的相似性,其功能未知。我们已经确定了N15, PY54, ko2和海洋端粒噬菌体VP58.5的预测抑制基因的转录起始位点。分析了这些基因对大肠杆菌、小肠结肠炎杆菌和副溶血性弧菌噬菌体繁殖的影响。结果表明,N15、ko2和PY54的抑制因子和反终止因子发挥了预期的活性。然而,虽然N15和ko2蛋白都影响N15的裂解和溶原性,但它们不影响PY54的繁殖。另一方面,各自的PY54蛋白只影响该噬菌体的繁殖。VP58.5的imb区含有2个显示前噬菌体抑制活性的基因,而裂解抑制基因未被鉴定。结果表明,这些温带噬菌体的生长调节机制具有意想不到的多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The diverse genetic switch of enterobacterial and marine telomere phages.

The diverse genetic switch of enterobacterial and marine telomere phages.

The diverse genetic switch of enterobacterial and marine telomere phages.

The diverse genetic switch of enterobacterial and marine telomere phages.

Temperate bacteriophages possess a genetic switch which regulates the lytic and lysogenic cycle. The genomes of the enterobacterial telomere phages N15, PY54 and ϕKO2 harbor a primary immunity region (immB) comprising genes for the prophage repressor, the lytic repressor and a putative antiterminator, similar to CI, Cro and Q of lambda, respectively. Moreover, N15 and ϕKO2 contain 3 related operator (OR) sites between cI and cro, while only one site (OR3) has been detected in PY54. Marine telomere phages possess a putative cI gene but not a cro-like gene. Instead, a gene is located at the position of cro, whose product shows some similarity to the PY54 ORF42 product, the function of which is unknown. We have determined the transcription start sites of the predicted repressor genes of N15, PY54, ϕKO2 and of the marine telomere phage VP58.5. The influence of the genes on phage propagation was analyzed in E. coli, Y. enterocolitica and V.parahaemolyticus. We show that the repressors and antiterminators of N15, ϕKO2 and PY54 exerted their predicted activities. However, while the proteins of both N15 and ϕKO2 affected lysis and lysogeny by N15, they did not affect PY54 propagation. On the other hand, the respective PY54 proteins exclusively influenced the propagation of this phage. The immB region of VP58.5 contains 2 genes that revealed prophage repressor activity, while a lytic repressor gene could not be identified. The results indicate an unexpected diversity of the growth regulation mechanisms in these temperate phages.

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