Avicenna journal of medical biotechnology最新文献

{"title":"Bioactive Silver Nanoparticles Coated with Curcumin Inhibit Angiogenesis through MMP-9 and Cox-2 Down-Regulation.","authors":"Tayebe Ramezani Farzin, Mohammad Nabiuni","doi":"10.18502/ajmb.v18i2.21507","DOIUrl":"10.18502/ajmb.v18i2.21507","url":null,"abstract":"<p><strong>Background: </strong>Inhibition of angiogenesis is an attractive approach in cancer therapy. Both curcumin and silver nanoparticles (AgNPs) have demonstrated anti-angiogenic properties; however, the the poor water solubility of Curcumin and the side effects of AgNPs adversely affect their activity.</p><p><strong>Methods: </strong>In this study, AgNPs coated with curcumin (Cur-AgNPs), was used to improve aqueous-phase solubility of curcumin and decrease the side effects of AgNPs. Afterwards, treatment with curcumin enhanced the anti-angiogenic activity of Cur-AgNPs. The nanoparticles were synthesized as both reducing and stabilizing agents. Evaluation of anti-angiogenesis was assessed <i>in vitro</i> using Human Umbilical Vein Endothelial Cells (HUVECs) and <i>in vivo</i> through the Chorioallantoic Membrane (CAM) assay. Data were analyzed by one-way ANOVA with Tukey's multiple comparison test.</p><p><strong>Results: </strong>Synthesized Cur-AgNPs have an average diameter of 39 <i>nm</i>, with spherical shapes and an absorbance peak at 450 <i>nm</i> in the UV-visible spectrum. Cur-AgNPs showed a negative zeta potential. EDAX and FTIR confirmed the conjugation of curcumin with AgNPs. <i>In vitro</i> anti-angiogenesis assays demonstrated that Cur-AgNPs reduced the viability of HUVECs, an Inhibitory Concentration (IC50) value of 13 <i>μg/ml</i>. DAPI and acridine orange/propidium iodide staining revealed a significant increase in apoptotic cells following treatment with Cur-AgNPs. The expression of <i>Matrix Metalloproteinase 9</i> (<i>MMP-9</i>) and <i>Cyclooxygenase-2</i> (<i>COX-2</i>) was also inhibited in treated cells. <i>In vivo</i> anti-angiogenesis assays using the CAM model showed significant decrease in the number, length and hemoglobin content of CAM blood vessels.</p><p><strong>Conclusion: </strong>Curcumin conjugated with AgNPs may represent a promising strategy to enhance the therapeutic potential of both AgNPs and curcumin. However, further investigations, particularly regarding safety and biocompatibility of Cur-AgNPs, is needed in this field.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"147-156"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteolin-Treated 4T1 Cell-Derived Exosomes as Novel Antiproliferative Agents <i>In Vitro</i> and <i>In Vivo</i>.","authors":"Pardis Heidari, Razieh Hosseini, Nika Nasihatgar","doi":"10.18502/ajmb.v18i2.21499","DOIUrl":"10.18502/ajmb.v18i2.21499","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is the most widespread malignancy among women worldwide. Luteolin, a flavonoid, has demonstrated anti-cancer effects by triggering apoptosis in tumor cells. Exosomes are gaining much attention for cancer therapeutic approaches due to multitude of beneficial effects. This study is aimed to investigate the possible potential of exosomes derived from luteolin-treated 4T1 cells to ameliorate tumor in comparison to luteolin treatment only.</p><p><strong>Methods: </strong>In this study, 4T1 cell culture was exposed to luteolin. Following exosome extraction, they were characterized using field emission scanning electron microscopy, dynamic light scattering and western blot analysis. MTT assay was performed in order to evaluate cell viability after exposure to different concentrations of luteolin and exosomes. An <i>in vivo</i> breast cancer model was induced <i>via</i> subcutaneous injection of 4T1 cells to the BALB/C mice. After 14 days, tumor volume was measured, and expression of RhoA and ERK mRNAs were quantified by Real Time PCR.</p><p><strong>Results: </strong>The MTT assay demonstrated that exosomes from luteolin-treated 4T1 cells at a concentration of 320 <i>μg/μl</i> reduced cell viability by approximately 70% in a dose-dependent manner. Tumor volume in the exosome-treated group decreased by 57% relative to the tumor group, while the luteolin-treated group demonstrated a 39% reduction. Furthermore, <i>RhoA</i> gene expression was substantially downregulated in the exosome-treated group, and exosomes were more effective than luteolin in reducing <i>ERK</i> gene expression.</p><p><strong>Conclusion: </strong>Exosomes derived from luteolin-treated 4T1 cells effectively suppress breast cancer cell growth by reducing 4T1 cell viability and by decreasing tumor volume and downregulating tumor-associated genes <i>RhoA</i> and <i>ERK</i>. These results propose a novel therapeutic strategy for breast cancer, highlighting the promising potential of exosomes as an efficient drug delivery system.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"96-103"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Anti-melanogenic Effect of Aqueous and Hydroalcoholic Extracts of <i>Nasturtium Officinale</i> on the B16F10 Cell Line.","authors":"Mohaddese Heydari, Masoud Sadeghi-Dinani, Fatemeh Shafiee","doi":"10.18502/ajmb.v18i2.21505","DOIUrl":"10.18502/ajmb.v18i2.21505","url":null,"abstract":"<p><strong>Background: </strong>This project aimed to evaluate the anti-melanogenic characteristics of <i>Nasturtium officinale</i> (<i>N. officinale</i>) by assessing the impact of both aqueous and hydroalcoholic extracts on the inhibition of cellular and mushroom tyrosinase enzymes, as well as the suppression of the melanin synthesis in B16F10 melanoma cells.</p><p><strong>Methods: </strong>The aerial components of <i>N. officinale</i> were subjected to extraction using distilled water: ethanol (7:3) through the maceration technique. The extract's phenolic compounds were quantified employing the Folin-Ciocalteu method. The evaluation of the safety profile of the extracts on B16F10 cells was done by the MTT assay. Subsequently, the melanin concentration in B16F10 cells, alongside the inhibitory effects on both mushroom and cellular tyrosinase, was assessed following treatment with the aforementioned extracts.</p><p><strong>Results: </strong>The aqueous and hydroalcoholic extracts exhibited no significant toxicity on B16F10 when compared to Phosphate-Buffered Saline (PBS). Additionally, there was no notable difference in the cytotoxic effects of extracts on the B16F10 cell line. Both extracts resulted in inhibition of cellular and mushroom tyrosinase, along with a decrease in melanin levels in B16F10 in a concentration-dependent manner. Ultimately, the total phenolic content in the aqueous and hydroalcoholic extracts was found to be approximately 14 and 30 <i>mg/g</i> of gallic acid, respectively.</p><p><strong>Conclusion: </strong>This <i>in vitro</i> investigation offers evidence supporting the skin brightening properties of <i>N. officinale</i> as an anti-melanogenic agent. Given its safety profile and absence of toxic effects on melanoma cells, it may be incorporated into the formulation of skin-brightening products following preclinical tests.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"131-136"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Alternating Magnetic Fields for Normalizing Blood Parameters and Restoring Renal, and Cardiac Function in Diabetic Mice.","authors":"Mokhamad Tirono, Farid Samsu Hananto","doi":"10.18502/ajmb.v18i2.21504","DOIUrl":"10.18502/ajmb.v18i2.21504","url":null,"abstract":"<p><strong>Background: </strong>In recent years, the number of adults aged 20-79 years living with diabetes has increased more than threefold. Currently, the treatment of diabetes typically involves the long-term use of chemical and herbal drugs. However, prolonged use of chemical drugs may lead to side effects that can be detrimental to health. Therefore, this study aims to normalize blood glucose levels and restore kidney and heart cells.</p><p><strong>Methods: </strong>The research was conducted using diabetic mice as experimental subjects. The treatment involved exposure to an alternating Magnetic Field with Magnetic Flux Densities of 0.3 and 0.6 <i>mT</i> for 20 <i>min/day</i> over five consecutive days. The frequencies of the applied Magnetic Fields were 50, 100, 150, and 200 <i>Hz</i>.</p><p><strong>Results: </strong>The results showed that the greatest reduction in blood glucose levels (92.11%) was observed at a frequency of 100 <i>Hz</i> and an Magnetic Flux Density of 0.6 <i>mT</i>. Meanwhile, the highest increase in hemoglobin levels (81.11%) occurred at a frequency of 150 <i>Hz</i> and a Magnetic Flux Density of 0.3 <i>mT</i>. Other parameters that experienced non-linear changes included cholesterol levels, blood viscosity, and erythrocytes count, glomerulus and kidney cell density, and heart cell density.</p><p><strong>Conclusion: </strong>The optimal effects of magnetic field exposure do not always occur at the same frequency or Magnetic Flux Density.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"121-130"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Novel Nucleic Acid Target for the Rapid and Specific Detection of <i>Mycobacterium Simiae</i> Using Comparative Genomic Analysis.","authors":"Reza Kamali Kakhki, Mohammad Abavisani, Kiarash Ghazvini, Hosna Zare, Noshin Hojatpanah, Jamal Falahi, Alireza Neshani","doi":"10.18502/ajmb.v18i2.21508","DOIUrl":"10.18502/ajmb.v18i2.21508","url":null,"abstract":"<p><strong>Background: </strong><i>Mycobacterium simiae</i> (<i>M. simiae</i>) is a non-tuberculous <i>mycobacterium</i> (NTM) that closely resembles <i>Mycobacterium tuberculosis</i> (<i>M. tuberculosis</i>) in clinical and biochemical characteristics, notably its niacin-positive phenotype. This similarity frequently leads to misdiagnosis and inappropriate treatment with first-line anti-tuberculosis drugs, to which <i>M. simiae</i> is frequently resistant. Current diagnostic methods are expensive or need complex equipment, highlighting the urgent need for a rapid, specific, and accessible molecular target to identify <i>M. simiae</i> accurately.</p><p><strong>Methods: </strong>In this study, a modified genome comparison method was applied to the complete reference genome of <i>M. simiae</i> (AP022568.1) in order to identify a putative species-specific nucleotide sequence. A conventional PCR assay was designed to amplify a 168-<i>bp</i> fragment within this target, designated MST601 (<i>M. simiae</i> Target, 601 <i>bp</i>). The analytical sensitivity [Limit of Detection (LOD)] was determined using serial dilutions of genomic DNA. The pilot evaluation of the assay was assessed using 10 well-characterized clinical isolates of <i>M. simiae</i>.</p><p><strong>Results: </strong>The MST601-PCR assay demonstrated high analytical sensitivity, with a limit of detection of ~10 <i>fg</i> (≈2 genome equivalents per reaction) of <i>M. simiae</i> genomic DNA. No cross-reactivity among the tested species was observed with any of the 10 non-target mycobacterial species tested. The assay successfully amplified the target sequence from all 10 clinical isolates. Sequencing of the amplicons revealed ≥99% identity to reference <i>M. simiae</i> strains in the GenBank database, validating the assay's accuracy.</p><p><strong>Conclusion: </strong>A species-specific nucleic acid target, MST601, facilitating the rapid and accurate detection of <i>M. simiae via</i> conventional PCR was presented. This assay provides a low-cost and accessible option for diagnostic laboratories.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"157-166"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monoclonal Antibodies and the Rise of Precision Immunopsychiatry.","authors":"Shahin Akhondzadeh","doi":"10.18502/ajmb.v18i2.21498","DOIUrl":"10.18502/ajmb.v18i2.21498","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"94-95"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Molecular Signature of Senescence in Mesenchymal Stem Cells.","authors":"Forough-Azam Sayahpour, Marjan Nejati, Mahya Rouhollahi-Masoumi, Mohamadreza Baghaban Eslaminejad, Sara Taleahmad","doi":"10.18502/ajmb.v18i2.21500","DOIUrl":"10.18502/ajmb.v18i2.21500","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal Stem Cells (MSCs) play a pivotal role in regenerative medicine due to their multipotency and immunomodulatory properties. However, during <i>in vitro</i> expansion, MSCs undergo senescence, characterized by a decline in proliferation, impairment of differentiation potential, and altered secretory profiles, which limits their therapeutic efficacy. This study aimed to identify novel molecular regulators and network-level interactions underlying MSC senescence through microarray analysis of the GSE7888 dataset, comparing early and senescent MSCs.</p><p><strong>Methods: </strong>A total of 4597 Differentially Expressed Genes (DEGs) were identified between early (passages 4-5) and senescent (passages 22-28) MSCs, with 2219 upregulated and 2379 downregulated. Key regulators such as <i>CDKN1A</i> (p21), <i>CDK4</i>, and <i>CDK6</i> were implicated in cell cycle arrest and the progression of senescence. Pathway analysis highlighted the mTOR, FoxO, and p53 signaling pathways as key regulators of stress responses, metabolism, and aging. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis highlighted cellular processes, including protein transport and intracellular signaling, while protein-protein interaction networks identified high-connectivity nodes such as <i>TP53</i>, <i>FOXO3</i>, and <i>MDM2</i>. Senescent MSCs displayed phenotypic changes, including altered morphology and the emergence of the Senescence-Associated Secretory Phenotype (SASP), which impaired regenerative potential.</p><p><strong>Results: </strong>The findings suggest that targeting the mTOR, FoxO, and p53 pathways could delay senescence and enhance MSC therapeutic potential. Interventions such as rapamycin and FoxO3 activators show promise in reversing senescence.</p><p><strong>Conclusion: </strong>Future research should explore small molecules and gene-based therapies targeting senescence pathways to improve MSC-based regenerative strategies. These results provide a foundation for developing innovative approaches to optimize MSC applications in clinical therapies.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 2","pages":"104-112"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13374677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence-Assisted CRISPR Gene Editing: Current Advances, Clinical Challenges, and Future Directions in Precision Medicine.","authors":"Mahdi Yousefian, Maryam Baharmast","doi":"10.18502/ajmb.v18i1.21041","DOIUrl":"10.18502/ajmb.v18i1.21041","url":null,"abstract":"<p><p>Recent advances in Artificial Intelligence (AI) have profoundly transformed the field of genome editing, particularly through integration with the Clustered Regularly Inter-spaced Short Palindromic Repeats (CRISPR) technology. This review highlights how AI-driven computational models are reshaping guide RNA (gRNA) design, off-target prediction, and editing precision in CRISPR-Cas systems. A PRISMA-informed literature survey was conducted using PubMed, Scopus, EMBASE, and Google Scholar databases to identify studies exploring AI-assisted CRISPR applications in gene therapy and biomedical research. The results demonstrate that deep learning, machine learning, and reinforcement learning approaches significantly enhance prediction accuracy, algorithmic efficiency, and translational potential across genetic diseases such as β-thalassemia, muscular dystrophy, and cancer. Moreover, ethical challenges, algorithmic bias, and data security concerns remain critical barriers to clinical adoption. This review also discusses the emerging landscape of AI-assisted CRISPR research in Iran, emphasizing national progress, infrastructural constraints, and future opportunities. Overall, the convergence of AI and CRISPR technologies promises to advance precision medicine by accelerating the development of personalized, efficient, and ethically responsible genome-editing solutions.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"3-15"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HLA Type and the Effect of HLA Antibodies in Kidney, Liver, and Pancreas Transplantation: A Review.","authors":"Edalat Zarei, Jamshid Roozbeh, Hamed Nikoupour Deylami, Saman Nikeghbalian, Mahbube Ahrami, Farukhruzi Nasrollozoda, Ahmad Hashemzehi, Masoud Hashemzaei","doi":"10.18502/ajmb.v18i1.21042","DOIUrl":"10.18502/ajmb.v18i1.21042","url":null,"abstract":"<p><p>Solid Organ Transplantation (SOT) has evolved from being an experimental procedure to a well-established therapeutic option for patients with end-stage organ failure. Among the most prevalent types of transplantation are liver, kidney, and pancreas transplants. Progress in surgical techniques and organ procurement has led to a decrease in complications, such as ischemic injury. Nevertheless, immune-mediated graft rejection continues to pose a significant challenge. The purpose of this review is to underscore the significance of Human Leukocyte Antigen (HLA) in the outcomes of SOT, particularly its critical role in donor-recipient matching, the risk of rejection, and the long-term survival of grafts. A comprehensive review of the relevant literature concerning the relationship between HLA and SOT was conducted, focusing on the function of Major Histocompatibility Complex (MHC) molecules, HLA typing, and the effects of HLA diversity on organ matching and clinical results. HLA typing serves as a fundamental element in assessing donor-recipient compatibility and minimizing the chances of graft rejection. The extensive polymorphism of HLA alleles, along with the existence of donor-specific antibodies, complicates the matching process, influences waiting periods, and impacts graft prognosis. Modulating HLA-mediated immune responses has the potential to enhance graft stability in liver, kidney, and pancreas transplantation. HLA molecules are crucial to the success of SOT. Ongoing clinical trials investigating novel immunosuppressive agents and HLA-targeted strategies may improve rejection management and long-term transplant outcomes. This review highlights the critical importance of HLA in liver, kidney, and pancreas transplantation.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"16-31"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sophoraflavanone G in Nano-Niosomal Form: Implications for Bacterial Inhibition, Biofilm Disruption, and Cancer Suppression.","authors":"Manouchehr Teymouri, Reyhaneh Khayyer, Milad Iranshahy, Reza Salarinia, Parastoo Zarghami Moghaddam, Ameneh Mohammdi, Toktam Memariani, Samaneh Mollazadeh","doi":"10.18502/ajmb.v18i1.21049","DOIUrl":"10.18502/ajmb.v18i1.21049","url":null,"abstract":"<p><strong>Background: </strong>Sophoraflavanone G, SG, is a flavonoid compound found in Sophora species with various biological properties, including antibacterial, anticancer, antibio-film activities. However, this compound shows limited solubility in water, which reduces its bioavailability and hinders its practical application. To overcome this barrier, SG nano-niosomal form was prepared.</p><p><strong>Methods: </strong>In the current study, a nano-niosomal form of SG was prepared using cholesterol (Chol) and Tween 20. Antibacterial and antibiofilm activities were assessed by disc and well diffusion and biofilm assays, respectively, while anticancer specificity was evaluated by MTT on KB and L929 cell lines.</p><p><strong>Results: </strong>Disc and well diffusion assays showed a reduction in planktonic antibacterial activity of niosomal SG compared with free SG, whereas biofilm assays improved anti-biofilm effects; MTT assays indicated reduced cytotoxicity toward L929 cells with retained activity against KB cancer cells, suggesting improved anticancer specificity.</p><p><strong>Conclusion: </strong>While niosomal formulation decreased SG's activity against planktonic bacteria, it enhanced antibiofilm effects and improved anticancer specificity by reducing toxicity to normal cells, making niosomal SG a promising candidate for cancer-directed therapeutic applications despite limited antimicrobial gains.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"61-68"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}