Avicenna journal of medical biotechnology最新文献

{"title":"Expression, Purification, and Application of SARS-CoV-2 Nucleocapsid Protein for Serological Detection of IgG and IgM Antibodies.","authors":"Saeideh Zamani Koukhaloo, Farshid Moosavi, Bahareh Zamani, Niloofar Agharezaee, Parisa Yousefi, Mahboobeh Nazari, Jafar Mahmoudian","doi":"10.18502/ajmb.v18i1.21046","DOIUrl":"10.18502/ajmb.v18i1.21046","url":null,"abstract":"<p><strong>Background: </strong>SARS-CoV-2 is a novel coronavirus that has caused dramatic loss of life and poses an unprecedented public health challenge worldwide. The nucleocapsid (N) protein of SARS-CoV-2 is the most abundant viral protein and a potent immunogen.</p><p><strong>Methods: </strong>In the current study, the <i>N</i> gene of SARS-CoV-2 was amplified from RNA extracted from a COVID-19 positive patient and then cloned into the pCold-I expression vector. The full-length His-tagged N protein was expressed in <i>Escherichia coli</i> (<i>E. coli</i>) using 0.5 <i>mM</i> IPTG and subsequently purified by nickel affinity chromatography. The purified N protein was characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Furthermore, the purified N protein was applied in SARS-CoV-2 IgG and IgM ELISA immunoassays.</p><p><strong>Results: </strong>The results showed that purification of the N protein in the presence of urea yielded a protein band of approximately 48 <i>kDa</i> on SDS-PAGE, corresponding to the full-length N protein. Additionally, Western blot analysis of the purified recombinant N protein showed a band of the same molecular weight. In the SARS-CoV-2 IgG ELISA assay, anti-N protein antibodies from a COVID-19 positive patient's serum successfully recognized the coated N protein. In the IgM ELISA test, an N-HRP conjugate was used in ELISA wells to reveal the interaction of HRP-conjugated N protein with pre-coated anti-N protein antibodies (IgM isotype).</p><p><strong>Conclusion: </strong>These results indicate that the expressed N protein of SARS-CoV-2 could serve as a valuable reagent for the development of antibody-based immunoassays to detect SARS-CoV-2 IgG and IgM antibodies.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translational Medicine and Biotechnology: Turning Discovery into Impact.","authors":"Ladan Kashani, Shahin Akhondzadeh","doi":"10.18502/ajmb.v18i1.21040","DOIUrl":"10.18502/ajmb.v18i1.21040","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Curcuma xanthorrhiza</i> Extract Modulates <i>CASP3</i> and <i>TIMP1</i> Expression and Regulates 8-OHdG, Collagen, and Protein Levels in UV-Induced BJ Cells.","authors":"Wahyu Widowati, Meilinah Hidayat, Rita Tjokropranoto, Dwi Nur Triharsiwi, Hanna Sari Widya Kusuma","doi":"10.18502/ajmb.v18i1.21045","DOIUrl":"10.18502/ajmb.v18i1.21045","url":null,"abstract":"<p><strong>Background: </strong>Ultraviolet (UV) radiation poses a significant health risk, particularly in high-exposure regions including Indonesia, contributing to more than 1.5 million UV-related Disability-Adjusted Life Years (DALYs) globally due to its involvement in photoaging, skin cancers, and chronic inflammation. This study aimed to evaluate the <i>Curcuma xanthorrhiza</i> extract (CXE) mitigating effects against UV-induced damage in human dermal fibroblasts (BJ cells) by assessing gene expression, protein integrity, DNA damage, and collagen levels.</p><p><strong>Methods: </strong>BJ fibroblasts were irradiated to UV radiation and given CXE at 3.13-12.5 <i>μg/ml</i> concentrations. <i>TIMP1</i> and <i>CASP3</i> gene expression were analyzed <i>via</i> qRT-PCR, while total protein, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and collagen content were measured using ELISA.</p><p><strong>Results: </strong>CXE treatment significantly upregulated <i>TIMP1</i> and downregulated <i>CASP3</i> expression in a concentration-dependent manner, with the strongest effects showed at 12.5 <i>μg/ml</i> (p<0.05). At the same concentration, CXE significantly restored total protein levels, reduced 8-OHdG accumulation, and preserved collagen content compared with the UV-induced control (p<0.05).</p><p><strong>Conclusion: </strong>These findings suggest CXE exerts reparative effects against UV-induced photoaging through antioxidant, anti-apoptotic, and Extracellular Matrix (ECM) preserving mechanisms, supporting its potential as a botanical anti-aging therapy.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> and <i>In silico</i> Analysis of <i>SCIN</i> rs376349889 as a Potential Biomarker for Gastric and Colorectal Cancers.","authors":"Neda Vaghefinezhad, Mansoureh Azadeh, Majid Tafrihi, Abasalt Hosseinzadeh Colagar","doi":"10.18502/ajmb.v18i1.21052","DOIUrl":"10.18502/ajmb.v18i1.21052","url":null,"abstract":"<p><strong>Background: </strong>Numerous research endeavors have reported altered expression of Scinderin (<i>SCIN</i>) in various cancer types. Single Nucleotide Polymorphisms (SNPs) represent the most prevalent form of genetic variation within the human genome which can have significant functional consequences, including cancer predisposition.</p><p><strong>Methods: </strong>This study investigated SNP-induced structural alterations in the SCIN protein and their potential effects on stability and function, using <i>in vitro</i> and <i>in silico</i> approaches. Integrating experimental and computational data provides insight into the role of this variant in tumorigenesis and highlights its potential as a molecular biomarker for cancer diagnosis and prognosis.</p><p><strong>Results: </strong>Out of 1,054 nonsynonymous SNPs (nsSNPs), 11 were consistently predicted to be deleterious. Among them, rs376349889 (R511G) was associated with decreased protein stability, loss of ADP-ribosylation at R511, disrupted ionic interactions, and increased hydrophobicity, all of which may impair SCIN function. Subsequently, genotyping of 200 colorectal cancer and 200 gastric cancer samples for the rs376349889 SNP was performed using High-Resolution Melting (HRM) technique in compared to a matched control group.</p><p><strong>Conclusion: </strong>The findings revealed a considerable difference in the allelic prevalence of the rs376349889 SNP between cancer patients and control samples. Notably, the GG genotype was linked to a higher susceptibility to both gastric and colorectal cancers (p<0.0001). These results suggest that rs376349889 may influence <i>SCIN</i>-related oncogenic mechanisms and could serve as a promising biomarker for identifying or evaluating the risk of gastrointestinal cancers at an early stage.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"79-93"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges of the Application of Emerging Neuroscience Technologies in Courts.","authors":"Hassan Bakhtiary, Shahriar Eslamitabar, Shirin Shiarzian, Ehsan Lame","doi":"10.18502/ajmb.v18i1.21043","DOIUrl":"10.18502/ajmb.v18i1.21043","url":null,"abstract":"<p><p>Significant advances in neuroscience have improved the ability of physicians to diagnose and manage neurological and psychiatric disorders in patients. The use of neuroscience evidence in criminal trials in developed countries has increased significantly in the last two decades. This rapid increase has raised questions among the legal and scientific communities about the effects that these technologies can have on judicial decision-makers. The role of neuroscience in criminal liability is a topic that has been discussed in recent years. The purpose of this article is to review the use of neuroscience evidence in the criminal justice system, as well as current research examining the effects of neuroscience evidence on judicial decision-makers in criminal cases. This review is warranted given legal and scientific concerns about the impact of potential bias. The present study was conducted and analyzed using a documentary method and with reference to research published in the last four years. Some argue that neuroscience is irrelevant in the criminal court, while others believe that it can help prove the lack of control of behavior by many criminals. However, the truth is likely somewhere in between, as certain types of neuroscience evidence may be useful and relevant in criminal trials. This article describes recent advances in neuroscience in the fields of functional neuroimaging and artificial intelligence \"deep learning\" algorithms, and examines the legal and ethical challenges and potential benefits and drawbacks.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"32-38"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Step-by-Step Preparation of Immobilized Recombinant <i>Staphylococcus aureus</i> Protein A (SpA): A Versatile Tool for Efficient Antibody Purification.","authors":"Mahboobeh Nazari, Zahra Salimzadeh, Amir-Hassan Zarnani, Roya Ghods, Ramin Ghahremanzadeh","doi":"10.18502/ajmb.v18i1.21044","DOIUrl":"10.18502/ajmb.v18i1.21044","url":null,"abstract":"<p><strong>Background: </strong>This study highlights the significance of using <i>Staphylococcus aureus</i> (<i>S. aureus</i>) Protein A (SpA) for antibody purification.</p><p><strong>Methods: </strong>The gene encoding Protein A was isolated from <i>S. aureus</i> and cloned into the pET-28a vector. Following transformation into <i>Escherichia coli</i> (<i>E. coli)</i> BL21, recombinant Protein A was expressed and purified using a nickel affinity resin.</p><p><strong>Results: </strong>The recombinant expression of protein A produced a yield of 50 <i>mg/L</i>, indicating a substantial production efficiency. The characterization of the recombinant protein through various ELISA tests confirmed its binding affinity to antibodies. Subsequently, the recombinant Protein A was immobilized on two different matrices: activated Sepharose 4B and amine-functionalized magnetic nanoparticles.</p><p><strong>Conclusion: </strong>The immobilization on magnetic nanoparticles presents a versatile alternative, offering the advantages of rapid separation, high surface area, and ease of handling. Magnetic nanoparticles facilitate automation and reduce processing time, making them particularly attractive for clinical and industrial applications. These immobilized forms were used to efficiently purify serum IgG, demonstrating the potential of Protein A as an effective tool for antibody isolation in biotechnological applications.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a Novel Immunotoxin against Prostate Cancer based on PE40 Toxin: An <i>In silico</i> Approach.","authors":"Sadaf Azdoo, Mortaza Taheri-Anganeh, Khadijeh Ahmadi, Abdolkarim Ghadimi Moghadam, Ahmad Movahedpour, Ali Jamshidi","doi":"10.18502/ajmb.v18i1.21050","DOIUrl":"10.18502/ajmb.v18i1.21050","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer, is the second most prevalent malignant tumor and fifth leading cause of cancer-related death among men worldwide. Patients suffer from adverse side effects and low efficacy of traditional therapeutic approaches. At present, cancer-targeted therapy is a fascinating strategy of cancer therapy re-search via employing immunotoxins, which is a fusion of a targeting molecule and a killer toxin that can recognize a specific antigen on cancerous cells and trigger cell death.</p><p><strong>Methods: </strong>This study used a prostate-specific scFv and a truncated version of Pseudomonas-exotoxin to design a novel immunotoxin. After the construct design, the construct's secondary structure, physicochemical features, and allergenicity were predicted by SOPMA, Protparam, and AllergenFP, respectively. Then, the 3D structure was built via ITASSER. ProSa-web and PROCHECK were used for structure validation. The 3D model was docked by Cluspro, and molecular dynamics was carried out by GROMACS.</p><p><strong>Results: </strong>The results showed that the average RMSF value for the PSMA receptor was 0.478 Å, and for the designed toxin was 0.292 Å. The low range of changes indicates the stability of the complex during the simulation.</p><p><strong>Conclusion: </strong>The present results indicate that the designed immunotoxin is structurally stable, non-allergenic, and capable of binding PSMA, suggesting it as a potential candidate for further experimental evaluation.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"18 1","pages":"69-78"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of CRISPR Sequences in Archaea and Bacteria with Eukaryotic microRNAs.","authors":"Reyhane Ramezani, Mandana Behbahani, Hassan Mohabatkar, Kimia Sarraf Mamouri, Fatemeh Hejazi","doi":"10.18502/ajmb.v17i4.20072","DOIUrl":"10.18502/ajmb.v17i4.20072","url":null,"abstract":"<p><strong>Background: </strong>This study explores repetitive Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sequences from the archaea <i>Acidianus sp.</i> and <i>Acidianus ambivalens</i> (<i>A. ambivalens</i>), as well as from the bacterium <i>Yersinia ruckeri</i> (<i>Y. ruckeri</i>). These sequences are compared with human microRNA (miRNA) sequences to investigate potential genetic similarities and disease associations.</p><p><strong>Methods: </strong>CRISPR sequences were retrieved from the CRISPR/Cas⁺⁺ database, and human miRNA sequences were obtained from miRBase. Sequence alignments were performed using BLASTn with an E-value threshold of 1e-5 to identify significant similarities. Genes associated with matched human miRNAs were identified through the HGNC and GeneCards databases. Further analyses included comparison with disease-associated miRNAs reported in human and mouse datasets.</p><p><strong>Results: </strong>In <i>Y. ruckeri</i>, alignments revealed similarities to miRNAs linked with genes such as <i>FOXO1</i>, <i>PTEN</i>, <i>PAX7</i>, and <i>DOCK3</i>, which are associated with lung cancer and muscular dystrophies. In <i>A. ambivalens</i>, aligned miRNAs corresponded to loci including <i>CHM13</i> and <i>GRCh38,</i> potentially linked to periembolic adenocarcinoma and mild pre-eclampsia. For <i>Acidianus sp.</i>, matches were observed with miRNAs associated with genes like <i>Irak2</i>, <i>NOS2</i>, <i>STAT1</i>, and <i>Numb</i>, which have been implicated in Psoriatic arthritis, Alzheimer's disease, Hepatocellular carcinoma, and Coronary artery disease.</p><p><strong>Conclusion: </strong>CRISPR sequences from these prokaryotes show notable similarities with human miRNAs, suggesting possible indirect links to genes involved in major diseases. These preliminary findings emphasize the need for further investigation into shared sequence motifs and their functional roles in host-pathogen interactions or evolutionary biology.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"258-276"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Expression of <i>ADAMTS2</i>, <i>CA1</i>, and <i>OLAH</i> in Immune Cells of Rheumatoid Arthritis Patients: Real-time PCR Study on <i>In-silico</i> analysis of Treated <i>vs.</i> Newly Diagnosed Patients.","authors":"Fatemeh Dehghan, Zahra Bazi, Mehrdad Aghaei, Masoomeh Gholizadeh, Romina Malakouti, Zahra Hesari","doi":"10.18502/ajmb.v17i4.20074","DOIUrl":"10.18502/ajmb.v17i4.20074","url":null,"abstract":"<p><strong>Background: </strong>Rheumatoid Arthritis (RA) is a chronic inflammatory joint disease. Current treatments often have limited efficacy and cause side effects due to their nonspecific action, while early diagnosis is challenging. This study combined bioinformatics and experimental methods to identify key genes and pathways involved in RA, aiming to discover novel therapeutic targets and diagnostic biomarkers.</p><p><strong>Methods: </strong>RNA-seq data from immune cells of RA patients and healthy donors (GSE117769) were analyzed with DESeq to identify Differentially Expressed Genes (DEGs). Affected pathways were explored using EnrichR, and druggable genes were identified through DGIdb and a literature review. Expression of candidate genes was validated in additional RA blood and synovium microarray datasets (GSE45291, GSE82107, GSE77298) using the GEO2R tool. Finally, RT-qPCR was used to measure the expression of selected genes in Peripheral blood Mononuclear Cells (PBMCs) from newly-diagnosed and chronic RA patients and controls, with associations to clinical features and diagnostic accuracy assessed. Synovial fluid of RA patients were stained with Giemsa.</p><p><strong>Results: </strong>Combined <i>in-silico</i> and experimental analysis demonstrated significant upregulation of <i>CA1, OLAH</i>, and <i>ADAMTS2</i> in the PBMCs of RA patients. However, only <i>ADAMTS2</i> showed high expression in the synovial tissue of these patients. While <i>OLAH</i> and <i>ADAMTS2</i> were predominantly overexpressed in newly-diagnosed cases, <i>CA1</i> levels were consistently elevated in both early and chronic stages of RA.</p><p><strong>Conclusion: </strong>This study identified <i>CA1, OLAH</i>, and <i>ADAMTS2</i> as being upregulated in RA, with <i>ADAMTS2</i> showing promise as a therapeutic target, suggesting it may also have potential as a candidate for diagnosis and treatment.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"286-292"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Synergy Between Mitochondrial Function and Innate Heat in Traditional Persian Medicine: A Modern Scientific Perspective on Thermoregulation.","authors":"Majid Nimrouzi, Masoud Hashemzaei","doi":"10.18502/ajmb.v17i4.20069","DOIUrl":"10.18502/ajmb.v17i4.20069","url":null,"abstract":"<p><p>This study investigates the synergy between Traditional Persian Medicine (TPM)'s concept of innate heat (Hararat-e-Gharizi) and modern mitochondrial thermoregulation. TPM emphasizes innate heat as essential for sustaining life, paralleling modern understandings of mitochondrial ATP production and heat generation. This integration occurs through mitochondrial biogenesis, proton leak (<i>via</i> uncoupling proteins), and <i>Reactive Oxygen Species</i> (ROS) signaling, which correspond to the TPM concept of heat sustaining vital functions. These findings may guide novel therapeutic strategies that integrate TPM principles with mitochondrial biology. A comprehensive review of historical TPM texts and modern literature was conducted, comparing innate heat with mitochondrial roles in thermoregulation and energy balance. Data from PubMed, Google Scholar, and Scopus were analyzed to explore mechanisms of heat production in both traditional and modern contexts. Findings demonstrated that TPM's innate heat correlates with mitochondrial biogenesis, heat generation <i>via</i> Uncoupling Proteins (UCP1), and ROS regulation. These concepts reflect TPM's understanding of maintaining bodily warmth for health and longevity. The relationship between Hararat-e-Gharizi and mitochondrial thermogenesis offers a bridge between ancient medicinal practices and modern cellular biology. Both emphasize the role of heat in maintaining homeostasis and preventing disease, with modern science validating TPM's holistic approach. Clarifying these mechanisms provides deeper insight into therapeutic implications, highlighting thermodynamic parallels and the role of ROS signaling as a novel framework for understanding disease etiology and treatment. This study bridges Traditional Persian Medicine and modern mitochondrial thermoregulation, introducing integrative perspectives for personalized healthcare. It also highlights thermodynamic parallels and ROS signaling as a novel framework for understanding disease etiology and treatment. This study underscores the relevance of TPM's innate heat in modern medicine, emphasizing the importance of mitochondrial efficiency in thermoregulation and overall health. Integrating these perspectives can enhance personalized therapeutic strategies for disease prevention and longevity.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"234-241"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12918707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}