Avicenna journal of medical biotechnology最新文献

{"title":"Challenges and Future Prospects of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) in Nanomedicine: A Focus on Toxicity, Imaging, and Theranostics.","authors":"Maryam Alvandi, Sahar Nosrati, Ramin Mansouri, Zahra Shaghaghi, Shahnaz Saednia, Sara Alipour, Farzad Fathi","doi":"10.18502/ajmb.v17i4.20070","DOIUrl":"10.18502/ajmb.v17i4.20070","url":null,"abstract":"<p><p>Superparamagnetic Iron Oxide Nanoparticles (SPIONs) have emerged as a pivotal tool in nanomedicine, offering potential in drug delivery, imaging, and targeted therapies. However, their application is challenged by issues such as cytotoxicity, uneven biodistribution, and biocompatibility. SPIONs are predominantly cleared through renal or hepatobiliary pathways, with size and charge playing critical roles in determining their fate. While smaller SPIONs optimize renal clearance, their propensity to agglomerate and activate macrophages may induce inflammatory responses. Radiolabeled SPIONs face additional challenges in molecular imaging and nuclear medicine. Emerging strategies, such as chelator-free radiolabeling and multi-component nanoparticles, aim to address these limitations by improving targeting specificity and enhancing biocompatibility. Looking forward, SPIONs hold immense potential in theranostics, particularly in integrating imaging with targeted drug delivery and therapies. Advances in synthesis and surface functionalization may enhance their safety and effectiveness. Future research should focus on optimizing SPIONs, integrating them with therapeutic agents, and improving targeting and clearance mechanisms. Collaboration among experts and the use of <i>Artificial Intelligence (AI)</i> modeling could accelerate their development for personalized treatment applications. This review uniquely highlights recent advances in radio-labeled SPIONs for molecular imaging and targeted therapy, addressing challenges like biocompatibility, stability, and translational applicability.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"242-248"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Modality Therapy: Synergistic Enhancement of Radio-Hyperthermia by Gold-Gold Sulfide Nanoparticles in MCF-7 Cells.","authors":"Mahdi Sadat-Darbandi, Amirhossein Fathabadi, Shabnam Oloomi, Ameneh Sazgarnia","doi":"10.18502/ajmb.v17i4.20076","DOIUrl":"10.18502/ajmb.v17i4.20076","url":null,"abstract":"<p><strong>Background: </strong>This study examines the synergistic impact of Gold-Gold Sulfide (GGS) nanoparticles combined with hyperthermia and radiotherapy on MCF-7 cancer cells. GGS nanoparticles, with strong near-infrared absorption and photothermal properties, enhance cellular sensitivity to radiotherapy.</p><p><strong>Methods: </strong>MCF-7 cells were treated with varying GGS concentrations and exposed to radiation doses of 50, 100, and 200 <i>cGy</i>, alongside laser irradiation for 10, 40, and 80 <i>s</i>. The IC₅₀ for GGS nanoparticles was approximately 350 <i>μM</i>.</p><p><strong>Results: </strong>Results revealed a significant reduction in cell viability with the combined GGS and laser exposure (p<0.001), demonstrating a synergistic effect in a dose-dependent manner. Further enhancement in cell viability reduction was observed when GGS nanoparticles were combined with both hyperthermia and radiotherapy (p<0.01).</p><p><strong>Conclusion: </strong>These findings suggest that GGS nanoparticles offer greater efficacy and reduced toxicity compared to gold nanoparticles, highlighting their potential for improving cancer therapy outcomes through combined hyperthermic and radiotherapeutic approaches.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"303-311"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-Based Recombinant Vaccines for Foot-and-Mouth Disease: A Meta-Synthesis.","authors":"Maziar Habibi-Pirkoohi, Afsaneh Mohkami","doi":"10.18502/ajmb.v17i4.20075","DOIUrl":"10.18502/ajmb.v17i4.20075","url":null,"abstract":"<p><strong>Background: </strong>Foot-and-Mouth Disease (FMD) remains a persistent global threat to livestock health and food security, particularly in endemic and resource-constrained regions. Conventional inactivated vaccines pose several challenges-including biosafety risks and dependence on cold-chain logistics. These limitations have prompted growing interest in plant-based recombinant vaccine platforms as innovative, scalable, and safer alternatives for FMD prevention.</p><p><strong>Methods: </strong>This study employed a qualitative meta-synthesis approach, guided by the Barroso-Sandelowski method, to systematically extract, interpret, and integrate findings from 35 peer-reviewed empirical studies published between 2000 and 2025. The selected studies focused on the development and evaluation of plant-made vaccines targeting FMD. Thematic coding and interpretive synthesis were applied to identify recurrent patterns, challenges, and opportunities across the literature.</p><p><strong>Results: </strong>The analysis yielded four dominant themes: (1) Platform Diversity: A variety of plant and algal expression hosts were used through transient or stable transformation systems, (2) Immunization Routes: Oral vaccination was noted for its logistical advantages and potential for mass immunization, though often requiring adjuvants to enhance immunogenicity, (3) Scale-Up Challenges: Key barriers included low recombinant protein yields, heterogeneity in post-translational modifications and high variability between production batches and (4) Regulatory Readiness: Despite encouraging pre-clinical data, most candidates have not progressed beyond experimental stages.</p><p><strong>Conclusion: </strong>Plant-based recombinant vaccines represent a promising frontier in the fight against FMD, offering novel avenues for safer, more accessible immunization strategies. However, their transition from bench to field remains hindered by technical limitations in expression and purification, as well as institutional and regulatory gaps.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"293-302"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barbed Wire Around Science: The Politicization of Knowledge.","authors":"Shahin Akhondzadeh","doi":"10.18502/ajmb.v17i4.20068","DOIUrl":"10.18502/ajmb.v17i4.20068","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"233"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12918706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiplasmodial Activity of Green-Synthesized MgO Nanoparticles Using <i>Achillea millefolium</i> Against Chloroquine-Resistant and-Sensitive <i>Plasmodium falciparum</i>.","authors":"Niloufar Khamsehpour, Haleh Hanifian, Mehdi Nateghpour, Ahmad Raeisi, Mohammad Shabani, Gholamreza Hassanpour, Aram Khezri, Seyed Ahmad Dehdast, Leila Farivar, Saeed Shahsavari","doi":"10.18502/ajmb.v17i4.20071","DOIUrl":"10.18502/ajmb.v17i4.20071","url":null,"abstract":"<p><strong>Background: </strong>Resistance to antimalarial medications, particularly in <i>Plasmodium falciparum</i> (<i>P. falciparum</i>), has emerged as a significant challenge, highlighting the need for innovative therapeutic strategies. Green-synthesized magnesium oxide nanoparticles (MgO NPs) represent a promising approach to therapeutic interventions. This study presents one of the first detailed evaluations of green-synthesized MgO NPs derived from <i>Achillea millefolium</i> (<i>A. millefolium</i>) against both chloroquine-sensitive (3D7) and chloroquine-resistant (K1) <i>P. falciparum</i> strains.</p><p><strong>Methods: </strong>In this study, MgO NPs were biosynthesized using <i>A. millefolium</i> extracts with varying solvent ratios. The nanoparticles were characterized using UV-Vis, FTIR, FESEM, and DLS techniques. Cytotoxicity was assessed <i>via</i> MTT and hemolysis assays. Their antiplasmodial efficacy was evaluated <i>in vitro</i> against chloroquine-sensitive (3D7) and -resistant (K1) <i>P. falciparum</i> strains.</p><p><strong>Results: </strong>The synthesized MgO NPs displayed quasi-spherical morphology and nanoscale size. Among tested formulations, the most effective showed IC₅₀ values of 0.17 <i>mg/ml</i> for the 3D7 strain and 0.76 <i>mg/ml</i> for the K1 strain, indicating significant antiplasmodial activity.</p><p><strong>Conclusion: </strong>Green-synthesized MgO NPs using <i>A. millefolium</i> demonstrated potent antiplasmodial activity at low IC₅₀ concentrations, showing efficacy against both chloroquine-sensitive and -resistant <i>P. falciparum</i> strains. These findings highlight their promise as plant-based nanotherapeutics for malaria treatment.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"249-257"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanosilver from Mangosteen Peel Extract (<i>Garcinia mangostana L.</i>) for Antibacterial Dental Preventive Agents.","authors":"Vinna Kurniawati Sugiaman, Rosalina Intan Saputri, Jeffrey Jeffrey, Wahyu Widowati, Mariska Elisabeth, Vini Ayuni, Dhanar Septyawan Hadiprasetyo","doi":"10.18502/ajmb.v17i4.20073","DOIUrl":"10.18502/ajmb.v17i4.20073","url":null,"abstract":"<p><strong>Background: </strong>Teeth are vital structures prone to issues such as caries and plaque formation, often caused by <i>Streptococcus mutans (</i>S. <i>mutans)</i>. This issue can be mitigated using natural ingredients like mangosteen fruit (<i>Garcinia mangostana</i> <i>L</i>.), especially its peel, is known for its medicinal benefits. However, its extract may take time to show effects, so it is being combined with nanosilver for improved drug distribution. To observe the antibacterial and antibiofilm potential of Mangosteen Peel Extract (MPE) in nanosilver form as a preventive agent in dentistry.</p><p><strong>Methods: </strong>The extraction was succeeded by a phytochemical assay and biosynthesis of MPE into Mangosteen Peel Extract Nanosilver (MPNs). Particle Size Analysis (PSA) and Transmission Electron Microscopy (TEM) were used to study this procedure. Disc diffusion tests were used to evaluate the antibacterial properties, and the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined. Furthermore, the antibiofilm activity against S. <i>mutans</i> was investigated.</p><p><strong>Results: </strong>the phytochemical contents in MPE were flavonoids, tannins, saponins, phenols, alkaloids, triterpenoids, and terpenoids. Particle size of MPNs was 126.1 <i>nm</i> and the Polydispersity Index (PDI) was 0.419. The highest antibacterial concentration as inhibition zone against <i>S. mutans</i> was 16.37±0.38 <i>mm</i> and 119.37±2.16% inhibitory activity, at the highest concentration (100%) p<0.05. The percentage of biofilm inhibition against <i>S. mutans</i> was 27.64-105.94% which was concentration dependent.</p><p><strong>Conclusion: </strong>MPNs has potential as an antibacterial and antibiofilm agent that can be used as a preventive agent in medicine.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 4","pages":"277-285"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12919320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Quality and Quantity of Nanoparticles Extracted from Human Adipose Tissue Derived-Mesenchymal Stem Cells.","authors":"Mobina Karimi, Banafsheh Heidari, Hanieh Jafary, Kavosh Zandsalimi","doi":"10.18502/ajmb.v17i3.19277","DOIUrl":"10.18502/ajmb.v17i3.19277","url":null,"abstract":"<p><strong>Background: </strong>Nanoparticles, as small extracellular vesicles, are considered promising tools in tissue engineering and regenerative medicine. This study aimed to investigate the effects of different processing and culture condition on the quality and quantity of extracts derived from human Adipose-Mesenchymal Stem Cells (AD-MSCs).</p><p><strong>Methods: </strong>AD-MSCs were proliferated in both the experimental and control groups. Nanoparticles were extracted from AD-MSCs-extracts and analyzed using SEM, TEM, DLS, Zeta potential, FTIR and BCA analyses. The morphological characteristics (shape, size, distribution, surface topography, and agglomeration/aggregation), structural appearance (poly-disperse intensity, colloidal particle behavior, surface charge, and stability), chemical properties (functional groups and ionic interactions) and total protein concentration were detected in the extracted nanoparticles. Additionally, the morphological characteristics, apoptosis, mitochondrial oxidoreductase activity, and migration potential of AD-MSCs in both groups were evaluated using acridine orange staining, MTT, and scratch assays.</p><p><strong>Results: </strong>In the experimental group, 100% of the nanoparticles had a diameter of 112.8± 25 <i>nm</i>, with the most frequency of 111.4 <i>nm</i>. However, in the control group, 72% of nanoparticles had a diameter of 350.2±43.6 <i>nm</i> with the highest frequency of 339.8 <i>nm</i> (p≤0.05). The Z-average, Poly-disperse intensity, and electrostatic stability of nanoparticles in the control and experimental groups were 171.9 <i>nm</i>, 0.727 and -0.000011 <i>cm²</i> <i>/Vs vs.</i> 103.7 <i>nm</i>, 0.205 and 0.000481 <i>cm²</i> <i>/Vs,</i> respectively (p≤0.05). In the experimental group, Zeta potential was -61.8 <i>mV</i>, which is in the range of ζ >-30<i>mV</i>. Although, Zeta potential in the control group was -1.5 <i>mV</i>, which is in the range of -30 <i>mV</i> <ζ <30 <i>mV</i> (p≤0.05). Total protein concentrations in the control and experimental groups were 11 and 41%, respectively (p≤0.05).</p><p><strong>Conclusion: </strong>Nanoparticles derived from AD-MSCs have high therapeutic applications in tissue engineering and regenerative medicine.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"186-195"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of a Unique Dextran Polymer-Conjugated Antibody and Horseradish Peroxidase Complex.","authors":"Bahareh Zamani, Niloofar Agharezaee, Farshid Moosavi, Saeideh Zamani Koukhaloo, Parisa Yousefi, Jafar Mahmoudian","doi":"10.18502/ajmb.v17i3.19276","DOIUrl":"10.18502/ajmb.v17i3.19276","url":null,"abstract":"<p><strong>Background: </strong>Immunohistochemistry (IHC) is a practical technique that utilizes the specific binding between an antigen and antibody to detect and localize specific antigens in tissue and cells. The optimal sensitivity in IHC is of utmost importance to achieve reliable results even when antigens are present at low abundance on the samples. Here, a dextran polymer labeled with Horseradish Peroxidase (HRP) and an antibody to improve the sensitivity of the IHC technique was synthesized.</p><p><strong>Methods: </strong>To this end, free thiol groups were introduced on sodium periodate-activated 30 <i>kDa</i> dextran using cystamine, followed by attachment of sulfo-MBS-activated goat anti-mouse antibody and sulfo-MBS-activated HRP to the activated dextran.</p><p><strong>Results: </strong>The production of Poly-HRP Antibody (PHA) was confirmed by the appearance of a new protein band exceeding 150 <i>kDa</i> on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Additionally, Enzyme-Linked Immunosorbent Assay (ELISA) and IHC techniques were employed to characterize PHA's functionality. The data demonstrated that PHA effectively detected target antigens in these assays.</p><p><strong>Conclusion: </strong>The newly synthesized PHA has the potential to provide a more sensitive platform for early detection of biomarkers in IHC. Further research is needed to evaluate its cost-effectiveness.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"180-185"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resorbable Bone-Fixation Materials: Synthesis, Physical-Chemical and Biological Properties.","authors":"Bakhtiniso Muzaffarova, Sherzod Eranov, Sanjar Tillayev","doi":"10.18502/ajmb.v17i3.19278","DOIUrl":"10.18502/ajmb.v17i3.19278","url":null,"abstract":"<p><p>Artificial bone materials were synthesized using the \"solvent casting method\" using polylactide/hydroxyapatite and various organic-inorganic modifiers. The physicochemical properties of the materials were studied using modern methods. IR spectroscopy showed that interactions between polymer macromolecules and hydroxyapatite occurred. When the powder was studied by the X-ray diffraction method, it was found to have an average crystallinity of 50-60%. When the textural properties were examined using SEM analysis, it was found that the introduction of magnesium phosphate into the samples resulted in the formation of porous particles with dimensions of 100-250 <i>μm</i>. This in turn, leads to the improvement of metabolic processes when the samples are introduced into living tissues. When the microhardness was determined by the Vickers method, it was found to be close to the hardness of natural bone, <i>i.e.</i> 27-34 HV. <i>In vitro</i> resorption was also performed in Simulated Body Fluids (SBF). Non-toxicity was observed when cytotoxic properties were studied. When the resorption process was studied <i>in vivo</i> in the upper third of the femur of rabbits, it was found that the ossification process of the samples was satisfactory after 28 days.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"196-207"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When Classrooms Become Battlegrounds: The Assault on Iranian Science.","authors":"Shahin Akhondzadeh","doi":"10.18502/ajmb.v17i3.19273","DOIUrl":"10.18502/ajmb.v17i3.19273","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"158"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}