Avicenna journal of medical biotechnology最新文献

{"title":"Swimming Exercise Attenuates DOX-Induced Cardiotoxicity by Modulating Apoptosis and DRP1/PGC1α/miR-23a Dependent Pathway in Rat Heart Tissue.","authors":"Hassan Darvakh, Saied Shakerian, Rohollah Ranjbar, Mohammad Reza Tabandeh","doi":"10.18502/ajmb.v17i3.19281","DOIUrl":"10.18502/ajmb.v17i3.19281","url":null,"abstract":"<p><strong>Background: </strong>Doxorubicin (DOX) is a widely used drug in cancer chemotherapy, but its cardiotoxicity limits its clinical applications. Combining exercise with chemotherapy offers a promising approach to mitigate the side effects of chemotherapy drugs. Limited information is available on the effects of swimming exercise on the molecular mechanisms related to DOX cardiotoxicity. This study aims to investigate the modulatory effect of swimming exercise on the apoptosis and <i>miR-23a</i>-dependent mitochondrial biogenesis and dynamics pathways in rat heart tissue treated with DOX.</p><p><strong>Methods: </strong>In this experimental study, thirty-two adult male Wistar desert rats (200-220 <i>g</i>) were randomly divided into four groups, including control, Doxorubicin (DOX; intraperitoneal injection of 5 <i>mg/kg</i> of DOX, once a week, for five weeks), swimming exercise (SE; water exercise for 60 <i>min</i>/day, five days a week, for six weeks) and DOX group along with Swimming Exercise (DOX-SE). At the end of the study, the cardiac expression of proteins related to apoptosis and mitochondrial biogenesis and <i>mir23-a</i> were analyzed using western blot and real-time PCR methods, respectively. One-way analysis of variance (ANOVA)with Tukey's post hoc test was used to analyze the data.</p><p><strong>Results: </strong>These findings revealed that DOX administration led to a significant decrease in the cardiac expression of PGC-1α and DRP-1 proteins and an increase in apoptotic proteins (caspase 3 and cytochrome C) compared to the control group (p<0.0001). Swimming exercise resulted in a significant increase expression in cardiac tissue of PGC-1α and DRP-1 proteins and a decrease in the expression of apoptotic proteins in the DOX-treated group (p<0.0001, p<0.01). Compared to the control group, the protein levels in the heart of the <i>miR-23a</i> were significantly increased in the DOX-treated group (p<0.001). However, exercise training attenuated the DOX-induced reduction in <i>miR-23a</i> expression gene in the cardiac muscle of DOX-treated mice (p<0.05).</p><p><strong>Conclusion: </strong>These findings suggest that swimming exercise may protect against DOX-induced cardiotoxicity by regulating apoptosis and DRP1/PGC1α/miR-23a pathway. This highlights exercise as a potential non-pharmacological strategy to mitigate chemotherapy-related heart damage.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"225-232"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term Inflammatory Exposure Affects Umbilical Cord-derived Mesenchymal Stem Cells Migration and Differentiation Through Modulation of NLRP3 Inflammasome Expression.","authors":"Helsy Junaidi, Dewi Sukmawati, Anoop Narayanan V, Jeanne A Pawitan, Sri Widia Jusman","doi":"10.18502/ajmb.v17i3.19279","DOIUrl":"10.18502/ajmb.v17i3.19279","url":null,"abstract":"<p><strong>Background: </strong>An innovative approach for tissue restoration using Umbilical Cord-derived Mesenchymal Stem Cells (UC-MSCs) is hindered by their poor survival rate due to the detrimental effects of the injured tissue microenvironment. Activation of NLRP3 inflammasome in an inflammatory environment which is followed by cellular impairment, has been reported. However, the expression of NLRP3 inflammasome in UC-MSCs in response to the inflammatory environment is not well understood. This study aims to investigate the impact of short-term exposure to an inflammatory environment induced by Lipopolysaccharide (LPS) on hUC-MSCs, focusing on cell viability, migration, differentiation, and the expression of NLRP3 inflammasome-related genes.</p><p><strong>Methods: </strong>hUC-MSC were exposed to LPS at concentration of 10 and 50 <i>μg/ml</i> for 3 and 6 <i>hr</i>. Cell viability was assessed using CCK-8 assay, migration capacity was evaluated using a scratch test, and differentiation capacity and the expression of NLRP3 inflammasome-related genes were measured using qRT-PCR.</p><p><strong>Results: </strong>Short-term LPS induction did not affect the viability of hUC-MSCs but reduced their migration and differentiation capacity, particularly at 50 <i>μg/ml</i> for both time points (p<0.05). The induction caused an increase in the mRNA levels of NLRP3, TLR-4, and RelA/p65, which correlated with elevated expression of caspase-1 and IL-1β.</p><p><strong>Conclusion: </strong>Short-term exposure to LPS influences hUC-MSCs by upregulating NLRP3, TLR4/ReIA (p65), IL-1β, and caspase-1 mRNA levels, leading to impaired migration and differentiation ability. This study underscores the significant impact of short-term exposure to an inflammatory microenvironment on hUC-MSC, potentially compromising their migration and differentiation capacity through the NLRP3 pathway.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"208-215"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorating Potential of Quercetin and Curcumin on Glucose-6-Phosphate Dehydrogenase Expression <i>via</i> miRNAs in Rats with Type 2 Diabetes Mellitus.","authors":"Mahsima Bagheri, Ameneh Khodarahmi, Fatemeh Zare Mehrjardi, Ali Moradi","doi":"10.18502/ajmb.v17i3.19280","DOIUrl":"10.18502/ajmb.v17i3.19280","url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes mellitus (T2DM) is accompanied by a significant risk of oxidative stress. While a link between T2DM and G6PD deficiency has been suggested, their interaction is not precisely understood. Furthermore, emerging evidence suggests an expression association between <i>G6PD</i> and <i>miR-1, miR-122,</i> and <i>miR-206</i>. Given the antioxidant and anti-inflammatory properties of Curcumin (Cur) and Quercetin (Q), This study aimed to assess the effects of curcumin and quercetin on G6PD expression and its correlation with the mentioned microRNA expression in liver, renal, heart, and muscle in rats with T2DM.</p><p><strong>Methods: </strong>RT-qPCR was employed to determine <i>miR-1</i>, <i>miR-122</i>, <i>miR-206</i>, and <i>G6PD</i> expression.</p><p><strong>Results: </strong>The findings revealed that curcumin and quercetin treatment elevated <i>G6PD</i> gene expression. Also, the treated groups exhibited down-regulation of <i>miR-1</i>, <i>miR-122</i>, and <i>miR-206</i> (p<0.05). Furthermore, there was a significant inverse correlation between <i>G6PD</i> and <i>miR-1</i> in heart, <i>miR-122</i> in all tissues except renal and <i>miR-206</i> expression in skeletal muscle and heart (p<0.05).</p><p><strong>Conclusion: </strong>This study suggests that curcumin and quercetin may prevent the development of T2DM by effectively increasing <i>G6PD</i> expression and reducing <i>miR-1</i>, <i>miR-122</i>, and <i>miR-206</i> expression.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"216-224"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Evaluation of the Anti-proliferative Activity and Hypersensitivity of L-Asparaginase from <i>Trichosporon asahii Isolate ChL11</i> and <i>Candida palmioleophila Isolate JK12</i>.","authors":"Tekeba Sisay, Naomi Maina, Victor Mobegi, Sabina Wachira","doi":"10.18502/ajmb.v17i3.19275","DOIUrl":"10.18502/ajmb.v17i3.19275","url":null,"abstract":"<p><strong>Background: </strong>L-Asparaginase is a crucial enzyme to treat Acute Lymphoblastic Leukemia (ALL), as it depletes L-asparagine, an essential amino acid for cancer cell survival. However, its clinical use is often restricted due to hypersensitivity reactions. This study examined the anti-proliferative effects and hypersensitivity of fungal L-asparaginases (L-ASNases) from <i>Trichosporon asahii isolate ChL11</i> (TaIChL11 L-ASNase) and <i>Candida palmioleophila isolate JK12</i> (CpIJK12 L-ASNase).</p><p><strong>Methods: </strong>The enzymes were produced and purified through ammonium sulfate precipitation, dialysis, and Sephadex G-100 chromatography, and tested on leukemia cells and BALB/c female mice to assess immune responses.</p><p><strong>Results: </strong>TaIChL11 L-ASNase had a molecular weight of 40 <i>kDa</i>, Michaelis constant (K_M) of 1.66×10^-2 <i>mM</i>, and V_max of 37.23 <i>mM/min</i>, while CpIJK12 L-ASNase had a molecular weight of 135 <i>kDa</i>, K_M of 2.3×10^-2 <i>mM</i>, and V_max of 14.03 <i>mM/min</i>. Both enzymes exhibited significant anti-proliferative effects against CCRF-CEM cancer cells, with half-maximal inhibitory concentration (IC₅₀) values of 2.74 <i>U/ml</i> for TaIChL11 LASNase and 3.30 <i>U/ml</i> for CpIJK12 L-ASNase after 48 <i>hr</i>, improving further after 72 <i>hr</i>. They also showed low cytotoxicity toward normal Vero E6 cells. <i>in vivo</i> studies demonstrated that TaIChL11 ASNase-treated mice had significantly lower Immunoglobulin (Ig) G levels than those treated with commercial L-ASNase from <i>Erwinia chrysanthemi</i> (Owenism) (p<0.005), with no detectable IgE response.</p><p><strong>Conclusion: </strong>These findings indicate that fungal L-ASNases, particularly TaIChL11 ASNase, with lower L-glutaminase activity and a favorable safety profile, could be promising alternatives to bacterial L-ASNases, potentially enhancing ALL treatment with fewer side effects.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"167-179"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and Characterization of IgY Polyclonal Antibodies Specific to Human Interleukin-6 and Their Neutralization Potential for Anti-inflammatory Responses.","authors":"Pacharaporn Khumpim, Charin Thawornkuno, Surapon Piboonpocanun, Wongsakorn Wongwadhunyoo, Promsin Masrinoul","doi":"10.18502/ajmb.v17i3.19274","DOIUrl":"10.18502/ajmb.v17i3.19274","url":null,"abstract":"<p><strong>Background: </strong>Interleukin-6 plays an essential role in cytokine storm and cytokine release syndrome, which occur in response to pathogen infection or tissue injury and are associated with severe symptoms. Neutralizing IL-6 can help reduce symptom severity. Chicken eggs serve as an excellent alternative antibody source compared to mammalian serum. The immunoglobulin Y (IgY) in the chicken's blood is transferred to and deposited within the egg yolk in large amounts. Several IgY products have been developed for therapeutic applications in various diseases. This study focuses on producing anti-human IL-6 (IL-6) IgY antibodies to support therapeutic advancements.</p><p><strong>Methods: </strong>Anti-IL-6 IgY was generated by immunizing three hens with recombinant IL-6 protein mixed with alum adjuvant, immunized four times at three-week intervals. Then, IgY was extracted from egg yolks. The specificity of IgY was determined by Western blot. The neutralizing activity against secreted IL-6 was demonstrated by Human Coronavirus OC43 (HCoV-OC43)-infected cells and Lipopolysaccharide (LPS)-stimulated human lung fibroblast MRC-5 cells.</p><p><strong>Results: </strong>The specific anti-IL-6 was detected starting from day 10 to day 90 after immunization. The average yield of total IgY was 17.97±15.66 <i>mg</i> per egg. The extracted anti-IL6 IgY antibody exhibited efficient neutralizing effects against secreted IL-6 in the HCoV-OC43-infected or LPS-stimulated cells in a dose-dependent manner.</p><p><strong>Conclusion: </strong>This study highlights the ease of production and the satisfactory yield of anti-IL-6 IgY derived from chicken eggs. The antibody demonstrates an <i>in vitro</i> inhibitory effect on IL-6, with potential applications in therapeutic development.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 3","pages":"159-166"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging the Skills Gap: Insights and Recommendations for Updating Medical Biotechnology Master's Curriculum in Iran.","authors":"Amirhossein Ahmadieh-Yazdi, Behzad Imani, Akram Jalali, Fahimeh Piryaei, Razieh Dalirfardouei","doi":"10.18502/ajmb.v17i2.18566","DOIUrl":"10.18502/ajmb.v17i2.18566","url":null,"abstract":"<p><strong>Background: </strong>Biotechnology is a rapidly developing field, and Iran aims to enhance its position in biosimilars through improved educational frameworks. Successful nations, such as the U.S., UK, Switzerland, China, and India, exemplify the positive impact of targeted educational programs in producing skilled graduates who advance the biotech sector. Research indicates a significant connection between curriculum relevance and graduate employability, suggesting that comprehensive curriculum assessment and reform can enhance student success and faculty involvement. This study investigated the necessity of revising the Master's curriculum in Medical Biotechnology in Iran to align with industry demands and global advancements.</p><p><strong>Methods: </strong>The current curriculum's effectiveness using the Delphi method was assessed to survey Master's students, professors, and industry stakeholders.</p><p><strong>Results: </strong>Findings revealed that while the current curriculum moderately aligns with educational objectives, significant gaps exist in practical training and resource availability. Many students reported feeling inadequately prepared for employment, particularly in essential skills such as animal cell culture, vaccine and monoclonal antibody design and production, and human skills like effective communication and teamwork. To address these deficiencies, some new courses focusing on practical experience and interdisciplinary approaches were recommended. Recommendations included enhancing laboratory facilities, integrating internships, and adopting team-based learning methods to improve student engagement and skill acquisition.</p><p><strong>Conclusion: </strong>Continuous investment in biotechnology education is crucial for maintaining competitive advantages in a globalized market. Overall, this research highlighted the importance of adapting educational programs to meet the dynamic needs of the biotechnology industry, ensuring graduates possess the necessary skills for successful careers in this field.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"147-156"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinase Polymerase Amplification (RPA)-ELISA as an Isothermal Molecular POCT Method for Bacterial Respiratory Infection Diagnosis.","authors":"Reza Azizian, Erfaneh Jafari, Babak Pourakabri, Setareh Mamishi, Reihaneh Hosseinpour Sadeghi, Maryam Sotoudeh Anvari","doi":"10.18502/ajmb.v17i2.18562","DOIUrl":"10.18502/ajmb.v17i2.18562","url":null,"abstract":"<p><strong>Background: </strong>Acute Respiratory Infections (ARIs) are a leading cause of childhood mortality worldwide, especially in African and Southeast Asian countries. Point of Care Test (POCT) techniques provide faster diagnoses compared to conventional or real-time PCR methods. Recombinase Polymerase Amplification (RPA) offers rapid on-site detection of these infections. Coupling RPA with Enzyme-Linked Immunosorbent Assay (ELISA) (RPA-ELISA) creates a cost-effective alternative, ideal for clinical applications. This study evaluates RPA-ELISA as a rapid diagnostic tool for bacterial respiratory infections.</p><p><strong>Methods: </strong>From 11 August 2022 to 9 February 2023, respiratory samples were collected and processed using culture methods, biochemical tests, real-time PCR, and RPA assays. The RPA reactions were conducted at 39<i>°C</i> for 30 <i>min</i>, and ELISA was used for detection. Statistical analyses focused on sensitivity, specificity, Positive Predictive Values (PPV), and Negative Predictive Values (NPV).</p><p><strong>Results: </strong>Forty-two respiratory samples, were collected in this period of which 10 samples showed no growth, and 32 tested positive. Among these positive samples, 15 isolates (35.7%) were identified as <i>Klebsiella pneumoniae</i> (<i>K. pneumoniae</i>), 14 isolates (33.3%) as <i>Streptococcus pneumoniae</i> (<i>S. pneumoniae</i>), and 3 isolates (7.1%) as <i>Moraxella catarrhalis</i> (<i>M. catarrhalis</i>). RPA-ELISA demonstrated 100% sensitivity for all pathogens, comparable to or better than RT-PCR, but had slightly lower specificity and PPV. RT-PCR achieved 100% specificity and PPV for all pathogens, indicating higher accuracy; yet, RPA-ELISA's sensitivity points to its effectiveness as a rapid screening tool.</p><p><strong>Conclusion: </strong>RPA-ELISA is significantly faster than real-time PCR and culture methods. Its ease of use makes it suitable for on-site diagnoses in resource-limited environments. Limitations include a small sample size for certain bacteria and the necessity for further validation in varied clinical contexts.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"114-121"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of <i>RfxCas13d</i> Expression in <i>Escherichia coli</i> Host using Response Surface Methodology.","authors":"Sepideh Abbaszadeh, Shahin Eghbalsaied, Meysam Soleimani, Sadegh Khazalpour, Saeid Afshar","doi":"10.18502/ajmb.v17i2.18563","DOIUrl":"10.18502/ajmb.v17i2.18563","url":null,"abstract":"<p><strong>Background: </strong>RfxCas13d, a key member of the Cas13 family, plays a vital role in CRISPR-based diagnostics for RNA sequence detection and gene silencing. This study aimed to enhance <i>RfxCas13d</i> expression by optimizing key parameters using Response Surface Methodology (RSM).</p><p><strong>Methods: </strong>The plasmid pET28b-RfxCas13d-His (Addgene 141322) was introduced into BL21 (DE3) and Rosetta™ (DE3) strains. Initial expression tests were conducted, followed by RSM-guided optimization of factors such as isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration, temperature, cell density at induction, and induction time in BL21 (DE3). Protein expression levels were quantified using ImageJ and AlphaEaseFC software to analyze band intensities.</p><p><strong>Results: </strong>BL21 (DE3) was selected for further optimization based on preliminary results. Analysis of 26 RSM-designed experiments revealed that temperature, induction time, IPTG concentration, and their interactions significantly influenced <i>RfxCas13d</i> expression. Optimal conditions were identified as 0.25 <i>mM</i> IPTG, an OD600 <i>nm</i> of 0.8 at induction, 37<i>°C</i>, and Overnight (ON) of induction. The regression model exhibited high accuracy, with a correlation coefficient of 0.97 and a p-value less than 0.05, confirming a strong linear relationship between predicted and observed values.</p><p><strong>Conclusion: </strong>This study highlights the significant impact of the four optimized factors on <i>RfxCas13d</i> expression. Under optimized conditions, a soluble protein concentration of 3.6 <i>mg</i>/100 <i>ml</i> cell culture was achieved after purification. It represents the first application of RSM for optimizing <i>RfxCas13d</i> expression, providing a foundation for further refinement of expression conditions. Continued use of RSM in future research will enhance the efficiency of RfxCas13d production for diagnostic and therapeutic applications.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"122-130"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity Study of Silver Nanoparticles Synthesized from <i>Suaeda Monoica</i> on Hep-2 Cell Line.","authors":"","doi":"10.18502/ajmb.v17i2.18567","DOIUrl":"https://doi.org/10.18502/ajmb.v17i2.18567","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"157"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Future of Iran's Population: Balancing Aging Trends and Fertility Rates.","authors":"Ladan Kashani, Shahin Akhondzadeh","doi":"10.18502/ajmb.v17i2.18558","DOIUrl":"10.18502/ajmb.v17i2.18558","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"82"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}