Avicenna journal of medical biotechnology最新文献

{"title":"Investigation of Anti-Cancerous Effects of <i>L. casei</i> -ATCC-393 and <i>L. rhamnosus</i>-GG on Apoptosis and Cell Cycle of B- CPAP Thyroid Cancer Cell line in Comparison to Fibroblast Cell Line.","authors":"Maryam Honardoost, Fatemeh Soleimanifar, Solat Eslami, Sara Cheraghi, Mohammad Ebrahim Khamseh, Maryam Darvish, Hamed Haddad Kashani","doi":"10.18502/ajmb.v17i2.18561","DOIUrl":"10.18502/ajmb.v17i2.18561","url":null,"abstract":"<p><strong>Background: </strong>Thyroid cancer is the most common type of cancer affecting the endocrine system. The main treatment approaches consist of surgical procedures and radioiodine therapy. Recently, there has been a heightened interest in investigating alternative treatment options, including probiotics, which could potentially minimize toxicity. Consequently, there is a growing imperative for research aimed at investigating the potential role of probiotics in the management of cancer.</p><p><strong>Methods: </strong>The B-CPAP cell line was maintained in culture and tested with different dilutions of two bacterial strains. Toxicity evaluations were performed using the MTT assay to identify appropriate concentrations. mRNA was extracted and analyzed via real-time PCR to measure the expression levels of the <i>Bcl-2</i>, <i>Bax</i>, and <i>P53</i> genes. Furthermore, changes in the cell cycle and the induction of apoptosis were examined using flow cytometry.</p><p><strong>Results: </strong>The supernatant derived from <i>Lacticaseibacillus casei</i> (<i>L. casei</i>) - ATCC-393 and <i>Lacticaseibacillus rhamnosus</i> (<i>L. rhamnosus</i>)-GG demonstrated a significant inhibitory effect on the growth of B-CPAP cancer cells. The findings indicated that the combination produced a more pronounced anti-cancer effect by enhancing the expression of pro-apoptotic genes while reducing <i>Bcl-2</i> gene expression in B-CPAP cells.</p><p><strong>Conclusion: </strong>The findings indicated a notable change in the expression of genes associated with apoptosis and modifications in the cell cycle. This implies that probiotics may enhance the efficacy of chemotherapy in treating thyroid cancer. In particular, <i>L. rhamnosus</i> and <i>L. casei</i> may play a beneficial role in the therapeutic process. Further research is required to investigate the direct impact of probiotics on thyroid function.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"106-113"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Role of hsa_circ_0052112 as a Potential Biomarker in Breast Cancer: Insights from Experimental and <i>In Silico</i> Analyses.","authors":"Mahdi Alizadeh, Mahdieh Salimi","doi":"10.18502/ajmb.v17i2.18565","DOIUrl":"10.18502/ajmb.v17i2.18565","url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) are important in tumorigenesis and cancer progression, highlighting their potential as biomarkers for diagnosis, prognosis, and treatment monitoring.</p><p><strong>Methods: </strong>This study consists of experimental and <i>in silico</i> phases. In the experimental phase, the expression of hsa_circ_0052112 in tumor and blood samples from 40 breast cancer women was analyzed, compared to the control group using Sybr Green real-time RT-PCR followed by total RNA extraction and cDNA synthesis. Statistical analysis was performed using the <i>beta-actin</i> gene as a normalizer, compared to the normal control group as a fold change. In the <i>in silico</i> phase, interactions among circRNA, RNA-Binding Proteins (RBPs), and microRNAs (miRNAs) were investigated using Interactome Database. The miRcancer database was utilized to assess breast cancer-related miRNAs linked to hsa_circ_0052112. Target miRNAs were identified with TargetScan and filtered for relevance through DisGeNET. K-means clustering grouped genes by expression patterns, visualized in Cytoscape to illustrate circRNA-miRNA-mRNA relationships. Hub genes underwent pathway enrichment analysis using Reactome database to determine their functional significance.</p><p><strong>Results: </strong>Data revealed a significant increase in hsa_circ_0052112 expression in both blood and tumour of breast cancer patients. This increase was especially pronounced in patients with estrogen, progesterone, and HER2 receptor positivity, as well as in advanced disease stages with lymph node involvement. Enrichment analysis of hub genes indicates their role in the PI3K/AKT signaling pathway.</p><p><strong>Conclusion: </strong>hsa_circ_0052112 shows promise as a multifaceted biomarker for breast cancer, enhancing diagnosis and prognosis; while supporting personalized treatment strategies. Further clinical validation is necessary to confirm its utility.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"136-146"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionary Regeneration Therapy Utilizing Dental Stem Cells and State-of-the-Art Nanotechnology Devices to Heal Injured Teeth and Tissues.","authors":"Mohammad Balfaki, Erfan Salimi","doi":"10.18502/ajmb.v17i2.18559","DOIUrl":"10.18502/ajmb.v17i2.18559","url":null,"abstract":"<p><p>Regenerative medicine is a field of pharmacy and medicine that focuses on stem cells and other methods such as nanoscience and biotechnology to stimulate the body's natural regenerative processes and repairing damaged tissues and organs to improve function and reduce pain. In this review article, focus is on Dental Stem Cells (DSC) and other cells regeneration in human body. The appropriateness of tissue-engineered therapies relying on the multipotent regenerative abilities of DSC is accompanied by significant challenges, as growth factors and epigenetic components are crucial for preserving their multipotency while being susceptible to a range of natural and environmental factors. Current evidence highlights the positive outcomes associated with select regenerative therapies; nevertheless, to provide further support, additional data must be gathered through standardized therapies and further studies. Organoids (3D cell culture) and nano scaffolds are also being explored as potential tools for regenerative therapies. Understanding the mechanisms that determine the behavior of these cells and how they interact will enable future generation therapies. Demonstrating promise, cell therapy is an alternative approach within regenerative medicine. Developmental factors like extracellular vesicle production are thought to mediate the regenerative response through paracrine effects in cell therapy, which is widely recognized.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"83-97"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monocytes and Macrophages as Unique Cellular Compartments Governing Non-Alcoholic Fatty Liver Disease and Inflammation.","authors":"Ghazale Hemmatian, Davoud Rostamzadeh, Kaveh Baghaei, Mahdi Shabani","doi":"10.18502/ajmb.v17i2.18560","DOIUrl":"10.18502/ajmb.v17i2.18560","url":null,"abstract":"<p><p>Non-Alcoholic Fatty Liver Disease (NAFLD) is a spectrum of liver diseases from simple steatosis to the most severe form of hepatocellular carcinoma. Liver injuries resulting from various factors, including viral infections, alcohol consumption, and metabolic disorders, trigger the activation of resident immune cells and the recruitment of circulating immune cells to the liver. This chronic inflammatory environment leads to tissue damage and the progression of liver fibrosis. Macrophages are highly versatile immune cells that play a dual role in fibrosis: they contribute to the progression of fibrosis (M1 and Ly6c^high macrophages) and its resolution (M2 and Ly6c^low macrophages). M1 macrophages and those with high surface expression of Ly6C exhibit pro-inflammatory characteristics, while M2 macrophages and myeloid cells with low expression of Ly6C mitigate inflammation and inhibit fibrosis progression. Environmental stimuli influence the complex mechanisms hepatic macrophages regulate the fibrosis they encounter. Kupffer cells initiate the inflammatory cascade and recruit monocyte-derived macrophages, which modulate the propagation of fibrosis and promote fibrinolysis. Additionally, hepatic macrophages interact with other cell types through exosomes, facilitating the transfer of cellular components that influence the outcome of liver fibrosis. In this review, the critical role of macrophages in inflammation-induced fibrosis and tissue restoration is discussed.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"98-105"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Pro-Apoptosis Induction of <i>Teucrium persicum</i> Ethyl Acetate Extract on MCF-7 Cells: An <i>In Vitro</i> Study.","authors":"Mehdi Moeil, Majid Tafrihi, Ehsan Nazifi, Maryam Radfar","doi":"10.18502/ajmb.v17i2.18564","DOIUrl":"10.18502/ajmb.v17i2.18564","url":null,"abstract":"<p><strong>Background: </strong><i>Teucrium persicum</i> (<i>T. persicum</i>) is a well-known Iranian endemic plant that grows in the southern regions of Iran. It is used as a tea to treat abdominal pains, hyperlipidemia, and diabetes in traditional Iranian medicine. It has been previously found that the methanolic extract of <i>T. persicum</i> exerts significant cytotoxicity and inhibitory effects on different cancer cells. This study aimed to investigate the effects of ethyl acetate extract of <i>T. persicum</i> on MCF-7 cells.</p><p><strong>Methods: </strong>The experiments included MTT, DAPI staining, and investigating the expression of <i>BAX</i> and <i>BCL2</i> genes. The extract had a significant cytotoxic effect on MCF-7 cells, with an IC₅₀ value of 50 <i>μg/ml</i> for 48 <i>hr</i>.</p><p><strong>Results: </strong>DAPI staining assays showed that the extract induced morphological changes, chromatin condensation, and nuclear fragmentation. Additionally, the ethyl acetate extract induced the expression of <i>BAX</i> and down-regulated <i>BCL2</i> genes.</p><p><strong>Conclusion: </strong>These findings suggest that <i>T. persicum</i> has strong cytotoxic properties and warrants further investigation.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 2","pages":"131-135"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How are Students Really Selected for a Ph.D.? The Impact of the Thesis and Solving the Country's Problems.","authors":"Shahin Akhondzadeh","doi":"10.18502/ajmb.v17i1.17671","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17671","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Induction of L-lysine-α-Oxidase from <i>Trichoderma Harzianum</i> Rifai by Metabolic Products of <i>Brevibacterium</i> sp. and the Improvement of Its Isolation and Purification Techniques.","authors":"Irina Smirnova, Ekaterina Neborak, Valeriy Shkinev, Victor Larichev, Yuri Shneyder, Ida Bashkirova, Elena Karimova, Lyudmila Gavrilyuk, Daria Baranova, Maria Ploskonos","doi":"10.18502/ajmb.v17i1.17676","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17676","url":null,"abstract":"<p><strong>Background: </strong>The enzyme L-lysine-α-oxidase from <i>Trichoderma harzianum</i> Rifai is a promising anticancer, antifungal and antibacterial agent. Intensive exploring of its physico-chemical properties and possible ways of application requires sufficient amounts of the protein which in turn depends on good techniques of cultivation of the microorganism producer, enzyme soft isolation and purification, and storage.</p><p><strong>Methods: </strong>An improved method has been suggested for isolation and purification of the enzyme. A specific combination of column sorbents was adapted and gradient elution with sodium chloride was applied to elevate the yield of the enzyme. The inductive influence of Metabolic Products (MP) of the <i>Brevibacterium</i> species, along with fungal metabolites of <i>Ulocladium</i> sp. and <i>Trichoderma</i> sp. was tested. The enzyme activity assay was based on the detection of oxidized dimethylbenzidine in a peroxidase reaction coupled with an L-lysine-α-oxidase reaction. Some enzyme properties were additionally explored.</p><p><strong>Results: </strong>The upgraded technique of isolation and purification resulted in a yield of enzyme of about 79%. All strains of <i>Brevibacterium</i> sp. proved to be potent enhancers of L-lysine-α-oxidase activity and concomitant activities. The induced enzyme appeared to be less specific but more thermostable. Possible application scopes for the enzyme with modified properties are discussed. Phosphate buffer solution (pH=5.6) appeared to be the best one for long-term storage of the enzyme.</p><p><strong>Conclusion: </strong>A significant inducing effect of MP of <i>Brevibacterium</i> sp. on L-lysine-α-oxidase has been detected, and its isolation and purification techniques have been improved.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"39-46"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic Activity of <i>Momordica charantia</i> Extracts Through Incretin Pathway in Streptozotocin-Nicotinamide Induced Diabetic Rat Depends on Dose Differences.","authors":"Muhammad Fadhol Romdhoni, Muchsin Doewes, Soetrisno Soetrisno, Ratih Puspita Febrinasari","doi":"10.18502/ajmb.v17i1.17677","DOIUrl":"10.18502/ajmb.v17i1.17677","url":null,"abstract":"<p><strong>Background: </strong>This study addresses the increasing prevalence of type 2 Diabetes Mellitus (T2DM) and the need for alternative, cost-effective medications. The research problem focuses on the need to further study the dose effectiveness of <i>Momordica charantia</i> Extracts (MCE) on T2DM parameters, including Glucagon-Like Peptide 1 (GLP-1), Dipeptidyl Peptidase 4 (DPP-4), alpha glucosidase, glucosetransporter 5 (GLUT5), and pancreatic tissue histopathology.</p><p><strong>Methods: </strong>The methodology employed an experimental research design with 30 Wistar rats divided into six groups, each receiving different inductions and doses. Parameters were measured using Elisa, and histological analysis of pancreatic tissue was conducted using HE staining.</p><p><strong>Results: </strong>The Kruskal-Wallis test revealed significant differences in each group for the GLP-1 (p=0.003). However, the DPP4 test suggested a lack of significant difference in each group (p=0.192), and the GLUT5 test showed insignificant changes between each group (p=0.119). The ANOVA analysis on alpha-glucosidase revealed no statistically significant differences among the groups (p=0.202). Additionally, a qualitative examination of the histological analysis of pancreatic tissue indicated an improvement in the condition of the pancreatic tissue.</p><p><strong>Conclusion: </strong>MCE can increase GLP-1 levels, lower DPP-4, lower alpha-glucosidase, and raise GLUT5. However, there are no significant differences between other groups and the morphology of pancreatic tissue in rat model T2DM at a dose of 300 <i>mg/kg.</i></p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"47-55"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of Nanochelating-Based Copper Nanoparticles on Burn Wound Healing in Mouse Model.","authors":"Hossein Rezvan, Seyed Masoud Zolhavarieh, Alireza Nourian, Elham Bayat, Somayeh Kalanaky, Saideh Fakharzadeh, Pegah Karimi, Maryam Hafizi, Mohammad Hassan Nazaran, Sahar Hamoonnavard","doi":"10.18502/ajmb.v17i1.17672","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17672","url":null,"abstract":"<p><strong>Background: </strong>The aim of the present study was to investigate the potential of Nanochelating-based copper to accelerate the wound healing process and prevent infection in burn wounds.</p><p><strong>Methods: </strong>Six to eight-week- old female BALB/c mice were burned with a 1 <i>cm²</i> heated copper plate on the left flank and then divided into four treatment groups, treated with C8 (nanochelating-based CuNPs), cold cream (supplementary materials) as a control drug, Silver Sulfadiazine and no treatment, respectively. Skin tissue samples were taken from the mice on days 0, 3, 8, 15 and 24. One piece was fixed in 10% neutral buffered formalin for pathological examination and the others were stored at -80°<i>C</i> until used for pro-inflammatory and growth factor gene expression.</p><p><strong>Results: </strong>The healing process in the group treated with 10 <i>mg/ml</i> C8 was significantly faster, and the survival rate of the mice in this group was significantly higher than in the other groups. The pro-inflammatory genes were expressed and down-regulated earlier in the C8 treated mice. Histopathology confirmed the higher cure rate in the group treated with 10 <i>mg/ml</i> C8 compared to other control groups.</p><p><strong>Conclusion: </strong>C8 has beneficial effects on the healing of burn wounds and the effective dose of this compound should be further investigated. The present study demonstrates the anti-inflammatory properties of nano-chelate-based copper particles' on mouse skin burns. This research opens up new possibilities in dermatology and burn therapy and highlights the potential of copper-based formulations in the treatment of burn injuries.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"2-13"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Preliminary Characterization of a Novel scFv against <i>SARS-CoV-2</i> : an Experimental and Computational Analysis.","authors":"Samaneh Jahandar-Lashaki, Safar Farajnia, Effat Alizadeh, Farzin Seirafi, Asghar Tanoumand, Mohammad Kazem Hosseini","doi":"10.18502/ajmb.v17i1.17679","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17679","url":null,"abstract":"<p><strong>Background: </strong>Since the initial outbreak, the <i>SARS-CoV-2</i> virus has continued to circulate and mutate, resulting in the emergence of new viral sublineages. Due to the lack of effective protection and therapeutic measures against these new variants, the virus is able to further evolve and diversify. This study aimed to screen a phage antibody library to identify monoclonal antibodies in single-chain variable fragment (scFv) format that target the Receptor Binding Domain (RBD) of different <i>SARS-CoV-2</i> strains. The newly discovered scFv has the potential for use as a diagnostic or therapeutic option against <i>SARS-CoV-2</i>.</p><p><strong>Methods: </strong>The RBD protein was produced, purified, and used as an antigen during biopanning. Six rounds of panning enriched RBD-specific phages and the binding affinity of binders were monitored by polyclonal phage ELISA. Subsequently, monoclonal phage ELISA was employed to identify specific binders. After sequence confirmation, the reactivity of the isolated anti-RBD scFv was evaluated. Additionally, bioinformatics tools determined the interaction between selected scFv and <i>SARS-CoV-2</i> strains.</p><p><strong>Results: </strong>The ELISA analysis demonstrated that the expressed RBD retains its structural integrity and effectively interacts with antibodies present in the sera of COVID-19 patients. Through screening a phage display library, a strong-binding scFv for RBD was discovered, which can effectively neutralize <i>SARS-CoV-2</i> and its novel variants.</p><p><strong>Conclusion: </strong>The findings of this study have led to the discovery of a novel scFv that effectively neutralizes <i>SARS-CoV-2</i> strains, offering immense potential for research and therapy purposes.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"64-79"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}