Avicenna journal of medical biotechnology最新文献

{"title":"Comparison of Anticancer Effects between Platinum Levetiracetam and Platinum Azidothymidine through the Expression of Biomarker Genes on Cancer Cell Lines.","authors":"Abdolreza Sabokrouh, Nasim Ghaffari, Zahra Karimi Tafreshi, Freshteh Atabi, Zohreh Sharifi, Seyed Khalil Shokouhi Mostavafi","doi":"10.18502/ajmb.v17i1.17674","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17674","url":null,"abstract":"<p><strong>Background: </strong>The utilization of biomarkers is a way to assess the efficacy of recently created anticancer drugs. MiRNAs, telomerase, and Bcl-2 are extensively utilized as biomarkers for this purpose. This study aims to evaluate the comparison of the newly synthesized platinum compounds such as Platinum Azidothymidine (Pt-AZT) with Platinum Levetiracetam (Pt-Lev) on HepG2 cancer cell lines <i>via</i> the biomarkers.</p><p><strong>Methods: </strong>In this study, cells were divided into four groups: Group A (HDF cells) were the normal negative control group, group B were HepG2 untreated cancer cells, and groups C and D were treated cancer cells with Pt-AZT and Pt-Lev, respectively. After evaluating the LC₅₀ for the drugs by MTT test, the relative gene expression of the biomarkers was determined by qPCR.</p><p><strong>Results: </strong>The results showed a significant decrease for antiapoptotic genes including miRNA-21 (5.1±0.014), telomerase (0.56±0.48), Bcl-2 (0.41±0.276) in group D, whereas in group C was more than group D for miRNA-21 (6.0±0.141), telomerase (3.49±0.231), Bcl-2 (4.93±0.276) also there was a significant increase in miRNA-122 (33.97±0.04) in group D, whereas in group C was (28.36±0.007) and so lower than group D. Most of the investigated groups showed a significant difference (p<0.05). In addition, there were widespread apoptotic regions in Pt-Lev treatment compared to Pt-AZT.</p><p><strong>Conclusion: </strong>The advantages of using Pt-Lev were more powerful anticancer effects on biomarkers through inhibition of antiapoptotic and stimulation proapoptotic factors and also lower side effects and lower drug resistance than Pt-AZT; therefore, it can be considered a more effective anti-cancer therapy.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"24-30"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repellency Effects, Cytotoxicity Assessment and Chemical Compositions of <i>Citrus limon</i> Seeds Extracts and Fractions Against <i>Anopheles gambiae sl</i> Mosquitoes.","authors":"Mukaram Akintunde Adeniyi-Akee, Kingsley Adibe Mbachu, Kolade Ibrahim, Paul Matthew Osamudiamen, Olapeju Oluyemisi Aiyelaagbe, Sunday Olakunle Idowu, Olajire Aremu Adegoke","doi":"10.18502/ajmb.v17i1.17678","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17678","url":null,"abstract":"<p><strong>Background: </strong><i>Citrus limon</i> seeds have limited exploration, compared to other parts. The increasing prevalence of mosquito menace prompted the investigation into the repellent effects of the seed n-hexane extract and fractions of <i>Citrus limon</i> (Rutaceae) against adult <i>Anopheles gambiae sl</i> mosquitoes. The cytotoxicity and chemical constituents of the 100% n-Hexane Fraction (HF) were also evaluated.</p><p><strong>Methods: </strong>Successive extraction was done with cold-maceration using, n-hexane, Ethyl Acetate (EA) and methanol. The three solvent extracts were screened against <i>Anopheles gambiae sl</i> mosquitos, using human bait method, at doses, 1.5, 2.5 and 5.0 <i>mg/ml</i> and with Deet and acetone used as positive and negative controls respectively. The n-hexane extract was further fractionated with column chromatography. Ten fractions (100% nhexane, 10, 20, 30, 40, 60, 80% EA in n-hexane, 100% EA, 50% methanol in EA, and 100% methanol) were collected, and all tested at a single optimized dose of 5 <i>mg/ml</i> for 2 <i>hr</i>. The cytotoxicity and the chemical constituents of HF were determined using brine shrimp lethality test and gas chromatography-mass spectrometry respectively.</p><p><strong>Results: </strong>N-hexane extract and HF, both at 5 <i>mg/ml</i>, showed the most protective effect of 97.52 and 89.40% respectively, compared with standard (Odomos Deet) 100% activity, with no significant difference (p>0.05) between the three. HF was toxic with LC₅₀ values of 29.2354 <i>ppm</i>. Thirty compounds representing 79.25% of HF were identified. Major compounds present in the fraction were heptadecanamine (8.31%), N-(3-methylbutyl) acetamide (7.89%), 2-ethyl- 1-hexanamine (6.65%).</p><p><strong>Conclusion: </strong>This promising repellent activity further justified the traditional usage of <i>Citrus limon</i> as mosquito repellent.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"56-63"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Disulfide Bridges in the Interaction of <i>E. coli</i> -Derived Recombinant SCARB2 and EV-A71's Capsid.","authors":"Hai-Vy Vo-Nguyen, Thuoc Linh Tran, Hieu Tran-Van","doi":"10.18502/ajmb.v17i1.17675","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17675","url":null,"abstract":"<p><strong>Background: </strong>Hand, Foot, and Mouth disease is an acute infectious disease caused by a group of enteroviruses, including <i>Coxsackievirus A16</i> and <i>Enterovirus 71</i>. EV-A71-causing disease can give rise to severe complications in children, leading to meningitis, encephalitis, and even death. A potential approach to prevent the virus spread is inhibiting the invasion of EV-A71 into target cells, thereby helping to prevent not only the spread of EV-A71 in the community but also lessen the risk of outbreaks. EV-A71 cell's receptor, human scavenger receptor class B member 2, SCARB2, was used as a trap to gather the virus and limit its spreading.</p><p><strong>Methods: </strong>In this study, the recombinant receptor was expressed using <i>Escherichia coli</i> (<i>E. coli</i>) system. SCARB2 proteins expressed from <i>E. coli</i> BL21(DE3), and <i>E. coli</i> SHuffle^® T7 Express were in inclusion bodies and subsequently refolded into soluble forms with recovery efficiencies of 57.57, and 82.2%, respectively. The presence of intramolecular disulfide bridges in the refolded SCARB2 was examined by SDS-PAGE in combination with Dithiothreitol (DTT). The two proteins were then utilized to evaluate the interaction with EV-A71 capsid by Indirect Enzyme-Linked Immunosorbent Assay (ELISA) at different pH levels to compare the adhesion efficiency.</p><p><strong>Results: </strong>The results showed that SCARB2 protein expressed from <i>E. coli</i> SHuffle^® T7 Express with disulfide bond modifications had better adhesion to the viral capsid. Notably, when the medium pH was lowered to acidic levels, the binding efficiency of recombinant receptors to the viral capsid increased.</p><p><strong>Conclusion: </strong>To our knowledge, this study reported for the first time the activity of SCARB2 under extreme pH conditions and also revealed the crucial role of disulfide bridges in the interaction with EV-A71's capsid. This finding contributed to the strategy using recombinant SCARB2 as a viral trap.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"31-38"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethical Considerations Emerge from Artificial Intelligence (AI) in Biotechnology.","authors":"Mahintaj Dara, Negar Azarpira","doi":"10.18502/ajmb.v17i1.17680","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17680","url":null,"abstract":"<p><p>The integration of Artificial intelligence (AI) in biotechnology presents significant ethical challenges that must be addressed to ensure responsible innovations. Key concerns include data privacy and security, as AI systems often handle sensitive genetic and health information, necessitating robust regulations to protect individuals' rights and maintain public trust. Algorithmic bias poses another critical issue; AI can reflect existing biases in training data, leading to inequitable healthcare outcomes. Transparency in AI decision-making is essential, as \"black box\" models hinder trust, especially in drug discovery and genetics. Ethical implications of genetic manipulation require careful scrutiny to define the limits of human intervention. Additionally, societal impacts must be considered to ensure equitable distribution of AI benefits, preventing the exacerbation of disparities. Engaging diverse stakeholders, including ethicists and policymakers, is vital in aligning these technologies with societal values. Ultimately, prioritizing ethics will allow us to harness AI and biotechnology's potential while safeguarding human rights and promoting equity.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"80-81"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Moringa oleifera</i> Docking to Estrogen Receptor α Ameliorates Placental and Brain Damage in Stressed Rats.","authors":"Odochi O Chukwu, Cordilia Iyare, Anthony C U Ezimah, Albert E Okorocha, Nwaeze G Konyefom, Nnaemeka T Asogwa, Nancy P Igwe","doi":"10.18502/ajmb.v17i1.17673","DOIUrl":"https://doi.org/10.18502/ajmb.v17i1.17673","url":null,"abstract":"<p><strong>Background: </strong>Stress during pregnancy significantly impacts offspring early physiological programming. Herbal remedies are frequently used by pregnant women to enhance their wellbeing. <i>Moringa oleifera</i> Leaf Extract (MoLE) is believed to have both anti-stress and antioxidant properties which can act as a Selective Estrogen Receptor Modulator (SERM) that regulate activities of estrogen, and can have different effects on different tissues. Goal of this study is to compile information on molecular docking analysis of phytochemicals found in MoLE targeting Estrogen Receptor-alpha (ER-α) and assess effects of MoLE administration on dam's and fetal brain tissues and placenta, during gestational stress.</p><p><strong>Methods: </strong>Phytochemical study of MoLE was determined using Gas Chromatography-Mass Spectrometry. Molecular docking technique was employed to predict aspects of interaction and binding affinities energy of bioactive phytocompounds in protein site of ER-α using autodock tools. 30 apparently healthy pregnant Albino-Wistar rats were randomly placed into 6 groups of 5 rats per group and exposed to Chronic Unpredictable Stress (CUS) protocol for two weeks, as follows: Group I (water and normal rat chow <i>ad libitum</i>), Group II (CUS protocol only), Group III (5 <i>mg/kg</i> body weight/day of MoLE), Group IV (10 <i>mg/kg</i> body weight/day of MoLE), Group V (CUS protocol +5 <i>mg/kg</i> body weight/day of MoLE), Group VI (CUS protocol +10 <i>mg/kg</i> body weight/day of MoLE).</p><p><strong>Results: </strong>This study found that 1-Propanol, 3,3'-oxy bis- and 1, 2, 3-Trimethyldiaziridine are most potent ligands for ER-α among all 41 compounds. Photomicrograph examination of tissues from stressed rats showed mild to severe alterations in histology. Consumption of MoLE during chronic stress showed mild to moderate protective effects.</p><p><strong>Conclusion: </strong>These findings suggest that 1-Propanol, 3,3'-oxy bis- and 1, 2, 3-Trimethyldiaziridine can be further investigated for development of novel therapeutics.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"17 1","pages":"14-23"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Importance of Biosecurity in Emerging Biotechnologies and Synthetic Biology.","authors":"Mahboubeh Soleimani Sasani","doi":"10.18502/ajmb.v16i4.16738","DOIUrl":"10.18502/ajmb.v16i4.16738","url":null,"abstract":"<p><p>The age of synthetic biology is ushering in new technologies for the advancement of society, human health, and agriculture. It appears that synthetic biology has integrated engineering paradigms into biological contexts. The combined use of new biotechnology and synthetic biology raises concerns about biosafety, biosecurity, and even cyberbiosecurity. For example, synthetic biology increases the possibility of designing, developing, and deploying pathogenic bioweapons in new and different ways than natural pathogens, as well as manipulating the genome. Evaluation of new technologies and platforms that enable creative or destructive manipulation of biological materials, systems, and organisms is important to identify potential security opportunities and vulnerabilities. This issue poses challenges to the medical community and civilian populations worldwide, creating a growing need to implement and enforce standardized biosafety and biosecurity regulations to protect humans, animals, plants, and the environment. It is critical to establish rules and management guidelines, provide strong leadership at the individual and institutional levels, and utilize established biosafety and biosecurity tools to mitigate the risks associated with synthetic biology. This review addresses the current state of synthetic biology, focusing on the concepts of biosafety, biosecurity, and cyberbiosecurity, as well as enhancing the standardization, regulation, and management of biosecurity in synthetic biology. In this review, the current situation in the Middle East region has been discussed and the challenges and opportunities encountered by synthetic biology researchers in this area is explored. The Middle East region is vulnerable to bioterrorism due to various factors. However, some countries in this strategically important region face challenges as they lack the necessary resources to effectively combat this significant global threat. These attacks are not limited to a specific border or area; they can affect multiple countries or have a global impact.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"16 4","pages":"223-232"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-Fluorouracil Effectively Depletes Tumor Induced Myeloid Derived Suppressor Cells in 4T1 Mammary Carcinoma Model.","authors":"Khadijeh Ramezani-Aliakbari, Seyed Amir Jalali, Maedeh Alinejad, Mahmood Jeddi-Tehrani, Mahdi Shabani","doi":"10.18502/ajmb.v16i4.16740","DOIUrl":"10.18502/ajmb.v16i4.16740","url":null,"abstract":"<p><strong>Background: </strong>Myeloid Derived Suppressor Cells (MDSCs) are capable of inhibiting both innate and adaptive immune responses and accumulate in the microenvironment of breast tumors. Hence, MDSC depletion by chemotherapeutic agents can improve clinical efficacy of cancer immunotherapy. The effects of 5-FU and doxorubicin agents on MDSC reduction in 4T1 breast cancer murine model were evaluated.</p><p><strong>Methods: </strong>5×10⁵ of 4T1 tumor cells were injected into mammary fat pad of BALB/c female mice. Tumor bearing mice were randomly divided into 4 groups: PBS receiving control group, doxorubicin receiving groups at doses of 2.5 and 5 <i>mg/kg</i>, and 5-FU receiving group at dose of 50 <i>mg/kg</i>. Doxorubicin and 5-FU agents were intraperitoneally administrated at three doses with 5-day intervals and five doses for three times a week, respectively. Then, on day 20 post tumor cells injection, spleens and tumors were isolated to determine frequency of CD11b⁺ Gr1⁺ MDSCs by flow cytometry analysis.</p><p><strong>Results: </strong>5-FU was able to reduce significantly both splenic and interatumoral MDSCs comparing to control group (p=0.0276 and p=0.0067, respectively). Also, Doxorubicin treatment at dose of 50 <i>mg/kg</i> was associated to a significant reduction of splenic MDSCs in comparison to untreated group (p=0.0382). However, only 5-FU injection led to inhibit notably tumor growth in comparison to control group (p=0.0139).</p><p><strong>Conclusion: </strong>Findings show that 5-FU has inhibitory effects on MDSCs and tumor growth in 4T1 tumor model. So, more investigations are needed to study combination of 5-FU with immune based approaches to enhance the efficacy of cancer therapies.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"16 4","pages":"244-250"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunogenic Consideration of a Designed Polypeptide Against Brucellosis Compared to RB51: An <i>In Vivo</i> Study.","authors":"Mina Saadat, Mojgan Bandehpour, Bahram Kazemi, Nariman Mosaffa","doi":"10.18502/ajmb.v16i4.16741","DOIUrl":"10.18502/ajmb.v16i4.16741","url":null,"abstract":"<p><strong>Background: </strong>Brucellosis in livestock and its transmission to humans through the consumption of contaminated dairy products is an important issue. The introduction of new approaches using immunogenic proteins against and diagnosing brucellosis is a serious issue in human health.</p><p><strong>Methods: </strong><i>Brucella abortus</i> contains five proteins including: MOXR family ATPase-α2, T9SS C-terminal target domain-containing protein, Cobyric acid synthase, Hypothetical protein, and VirB11 type IV Secretion protein, which were considered and the designed recombinant polypeptide was produced and evaluated. The pure recombinant protein ABOR with 549aa in combination with chitin as an adjuvant was injected subcutaneously into guinea pigs to evaluate their immunity responses.</p><p><strong>Results: </strong>The results indicated that the ABOR recombinant protein induced Th1 immunity with high levels of specific IgG (IgG2a) as well as Interferon-γ (IFN-γ), Interleukin-2 (IL-2), IL-12, and Tumor Necrosis Factor-alpha (TNF-α), compared to the control group. Th1/Th2 ratio analysis demonstrated the efficacy of ABOR protein combined with chitin in stimulating cellular immunity in the animals.</p><p><strong>Conclusion: </strong>Designed recombinant polypeptide combined with chitin showed ability for induction of cellular and humoral immunity an guinea pigs compared to RB51 vaccine.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"16 4","pages":"251-259"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential of Human Wharton's Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application.","authors":"Wahyu Widowati, Ahmad Faried, Rimonta Febby Gunanegara, Fanny Rahardja, Fadhilah Haifa Zahiroh, Annisa Firdaus Sutendi, Faradhina Salfa Nindya, Rizal Azis, Renandy Kristianlie Ekajaya","doi":"10.18502/ajmb.v16i4.16739","DOIUrl":"10.18502/ajmb.v16i4.16739","url":null,"abstract":"<p><strong>Background: </strong>Diabetic Foot Ulcer (DFU) might be worsened by neuropathy and vascular issues. This condition can cause 14.3% fatality, stressing the need for effective wound healing therapy. Wound healing is a complex biological process, and human Wharton's Jelly Mesenchymal Stem Cells (hWJMSCs) may help manage DFU treatment issues. This research focuses on utilizing a gel carrier to deliver bioactive substances from Wharton's Jelly Mesenchymal Stem Cells secretome (hWJ-MSCs-Sec) as a possible treatment for DFU.</p><p><strong>Methods: </strong>To maintain quality, hWJMSCs-Sec is thoroughly mixed with carbomer gel and freeze-dried. ELISA test is performed to determine the characterization of the gel of hWJMSCs-Sec such as Keratinocyte Growth Factor (KGF), Platelet-Derived Growth Factor (PDGF), Hepatocyte Growth Factor (HGF), Epidermal Growth Factor (EGF), and Heparin-Binding EGF-Like Growth Factor (HB-EGF). The antioxidant activity was also measured with Hydrogen peroxide (H₂O₂), Nitric oxide (NO), and Ferric Reducing Antioxidant Power (FRAP) assay. Proliferation assay was utilized using WST-8 and the wound healing potential was assessed <i>via</i> the migration cell ability of scratched-human skin fibroblast (BJ cells).</p><p><strong>Results: </strong>The freeze-dried hWJ-MSCs-Sec showed higher levels of KGF, HGF, PDGF, EGF, HB-EGF, and the antioxidant activities compared to fresh hWJ-MSCs-Sec. Additionally, the gel of freeze-dried hWJ-MSCs-Sec exhibited higher levels compared to the gel of fresh hWJMSCs-Sec. This was evidenced by faster closure of scratched wounds on BJ cells treated with hWJMSCs-Sec and freeze-dried hWJ-MSCs-Sec gel.</p><p><strong>Conclusion: </strong>The freeze-dried hWJ-MSCs-Sec gel exhibits superior quality compared to the non-freeze-dried hWJ-MSCs-Sec gel. This demonstrates that the freeze-drying procedure can maintain the bioactive chemicals found in hWJMSCs-Sec, potentially enhancing the efficacy of this gel in promoting cell regeneration for wound healing.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"16 4","pages":"233-243"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Simulated Physiological Oocyte Maturation (SPOM) and L-Carnitine on Bovine Oocyte Developmental Competence.","authors":"Ali Malekpour, Abolfazl Shirazi, Sara Borjian Boroujeni, Ali Sarvari, Mohammad Mehdi Naderi, Mostafa Pournourali, Bahareh Behzadi, Mohammad Mehdi Mehrazar","doi":"10.18502/ajmb.v16i4.16742","DOIUrl":"10.18502/ajmb.v16i4.16742","url":null,"abstract":"<p><strong>Background: </strong>Simulated Physiological Oocyte Maturation (SPOM) mimics <i>in vitro</i> the physiological events of oocyte maturation in the presence of cAMP modulators. These modulators increase the intracellular concentrations of cAMP, which inhibits the immediate resumption of meiosis and gives the oocyte more time to gain optimal developmental competence. In addition, L-carnitine helps to increase the energy supply of cells through the β-oxidation of fatty acids. This study aimed to investigate the effect of SPOM and L-carnitine supplementation during <i>In Vitro Maturation</i> (IVM) and <i>In Vitro</i> Culture (IVC) on the developmental competence of bovine oocytes.</p><p><strong>Methods: </strong>Ovarian Cumulus Complexes (COCs) were cultured in the presence or absence of forskolin+IBMX during the first 2 <i>hr</i> of IVM (pre-IVM) with or without L-carnitine (LC) during IVM or IVC in six experimental groups as follows: I) pre-IVM (pre-IVM group), II) pre-IVM with L-carnitine supplementation during IVM (pre-IVM/LC group), III) L-carnitine supplementation during IVM (IVM/LC group), IV) L-carnitine supplementation during <i>in vitro</i> culture (IVC/LC group), V) pre-IVM+ IVC/LC group, and VI) no treatment during IVM and IVC (Control group). The cleavage and blastocyst rates, the blastocysts' total cells number, and the expression of <i>Nanog</i>, <i>Bax</i>, <i>Oct4</i>, <i>Cdx2</i>, and <i>Ifnt</i> genes in resulting blastocysts were assessed. To assess differences among experimental groups, a one-way analysis of variance was initially employed, followed by post hoc Fisher LSD. The difference between groups was considered statistically significant when p<0.05.</p><p><strong>Results: </strong>The cleavage and blastocyst rates in the Pre-IVM and Pre-IVM/LC groups was higher than control group and other groups (p≤0.05) except for IVC/LC and IVM/LC groups, respectively. The number of blastocyst's Inner Cell Mass (ICM) in pre-IVM and Pre-IVM/LC groups as well as the ratio of ICM/TE were higher than control group (p<0.05). The expression of OCT4, CDX2, and IFNT increased in both the pre-IVM and pre-IVM/LC groups compared to the control group (p<0.05).</p><p><strong>Conclusion: </strong>In conclusion, the application of SPOM-adapted IVM and L-carnitine during IVM of bovine oocyte improves the quantity and quality of the resulting embryos.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"16 4","pages":"260-267"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}