Anti-Quorum Sensing and Anti-Biofilm Activity of Ginger (Zingiber officinale) Rhizomes against Multidrug-Resistant Clinical Isolates of Pseudomonas aeruginosa

Q3 Biochemistry, Genetics and Molecular Biology

Avicenna journal of medical biotechnology Pub Date : 2023-11-27 DOI:10.18502/ajmb.v16i1.14171

Pankaj Kumar Sagar, Poonam Sharma, Rambir Singh

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Abstract

Background: The aim of this study was to determination of Anti-Quorum Sensing (AQS) and anti-biofilm potential of the methanol extract of ginger (Zingiber officinale) rhizomes against multidrug-resistant clinical isolates of Pseudomonas aeruginosa (P. aeruginosa). Methods: The AQS activity of ginger was determined against Chromobacterium violaceum (C. violaceum) ATCC 12472 (CV12472), a biosensor strain, in qualitative manner using the agar well diffusion method. The violacein pigment inhibition was assessed to confirm AQS activity of ginger. The AQS potential of sub-minimum Inhibitory Concentrations (sub-MICs) of the ginger extract was determined by targeting different QS regulated virulence factors, including swarming motility (using swarm diameter measurement method), pyocyanin pigment (using chloroform extraction method), Exopolysaccharide (EPS) (using phenol-sulphuric acid method), and biofilm formation (using microtiter plate assay), against clinical isolates (CIs 2, 3, and 4) and standard reference strain of P. aeruginosa (PA01). Results: The AQS activity of methanol extract of ginger was confirmed against C. violaceum (CV12472) as inhibition of violacein pigment formation without effecting the growth of CIs and PA01 of P. aeruginosa. The ginger extract exhibited concentration-dependent inhibition of virulence factors and biofilm formation. The maximum reduction was found in swarming motility, pyocyanin, EPS and biofilm formation against PA01 (51.38%), CI3 (57.91%), PA01 (63.29%) and CI2 (64.37%), respectively at 1/2 MIC of ginger extract. Conclusion: The results of present study revealed the effective AQS and anti-biofilm potential of Zingiber officinale rhizome methanol extract at a reduced dose (sub-MICs). The extract may be explored further for its antibiotic potential and also for reducing the chances of emergence of resistance in P. aeruginosa.

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生姜（Zingiber officinale）根茎对耐多药铜绿假单胞菌临床分离株的抗法定量感知和抗生物膜活性

背景：本研究旨在确定生姜（Zingiber officinale）根茎甲醇提取物的抗法定人数感应（AQS）和抗生物膜潜力，以对抗临床分离的耐多药铜绿假单胞菌（P. aeruginosa）。方法：采用琼脂井扩散法，定性测定生姜对生物传感器菌株绿脓杆菌（C. violaceum）ATCC 12472（CV12472）的 AQS 活性。为了确认生姜的 AQS 活性，还评估了其对暴马毒素色素的抑制作用。针对临床分离株（CIs 2、3 和 4）和铜绿假单胞菌标准参考菌株（PA01）的不同 QS 调控毒力因子，包括蜂群运动（使用蜂群直径测量法）、焦花青素色素（使用氯仿萃取法）、外多糖（使用酚硫酸法）和生物膜形成（使用微孔板检测法），确定了生姜提取物亚最低抑制浓度（sub-MICs）的 AQS 潜力。铜绿假单胞菌标准参考菌株（PA01）。结果生姜甲醇提取物的 AQS 活性被证实可抑制 C. violaceum (CV12472)的violacein色素形成，但不会影响铜绿假单胞菌的 CIs 和 PA01 的生长。生姜提取物对毒性因子和生物膜形成的抑制作用呈浓度依赖性。在生姜提取物浓度为 1/2 MIC 时，对 PA01（51.38%）、CI3（57.91%）、PA01（63.29%）和 CI2（64.37%）的蜂拥运动、焦花青素、EPS 和生物膜形成的抑制率最大。结论本研究结果表明，生姜根茎甲醇提取物在低剂量（亚 MICs）下具有有效的 AQS 和抗生物膜潜力。可进一步研究该提取物的抗生素潜力，并降低铜绿假单胞菌产生抗药性的几率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Avicenna journal of medical biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

2.90

自引率

0.00%

发文量