Assay and drug development technologies最新文献_第4页

Acknowledgment of Reviewers 2023. 鸣谢 2023 年审稿人。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-12-20 DOI: 10.1089/vio.2023.29036.ack

引用次数: 0

Liver-Targeting Composite Nanocarrier Delivery System Based on Chitosan Nanoparticles and Phospholipid Complexes. 基于壳聚糖纳米颗粒和磷脂复合物的肝脏靶向复合纳米载体输送系统

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-11-01 DOI: 10.1089/adt.2023.051

Fanming Kong, Jingmeng Sun, Yue Hu, Wenkai Huo, Dongdong Li, Weiyu Zhang

{"title":"Liver-Targeting Composite Nanocarrier Delivery System Based on Chitosan Nanoparticles and Phospholipid Complexes.","authors":"Fanming Kong, Jingmeng Sun, Yue Hu, Wenkai Huo, Dongdong Li, Weiyu Zhang","doi":"10.1089/adt.2023.051","DOIUrl":"10.1089/adt.2023.051","url":null,"abstract":"Liver fibrosis is mainly caused by excessive accumulation of extracellular matrix and structural changes in the liver, ultimately leading to cirrhosis if left untreated. Reducing hyaluronan synthesis by inhibiting hyaluronic acid deposition or regulating the expression of hyaluronic synthase can ameliorate liver fibrosis symptoms. In this study, we aimed to improve the bioavailability and liver-targeting capacity of hydroxymethyl coumarin (4-MU) using a newly developed phospholipid complex chitosan nanoparticle (4-MU PC/CNP) optimized using the Box-Behnken design. The composite nanocarrier delivery system was formulated using solvent evaporation technology, and formulation and process parameters were evaluated. Furthermore, 4-MU PC/CNPs and their pharmacokinetics were characterized. The established 4-MU PC/CNPs had an average particle size of 153.07 ± 0.29 nm, a polydispersity index value of 0.383, and a positive zeta potential of ∼35.4 mV. Compared with 4-MUs, 4-MU PC/CNPs exhibited significantly improved water solubility, faster plasma clearance and tissue distribution, and better liver targeting. Pharmacokinetic analysis showed that the oral bioavailability of 4-MU in 4-MU PC/CNPs was significantly higher than that of simple 4-MU. In conclusion, 4-MU PC improved drug lipid (oil-water distribution coefficient of 1.31 ± 0.03) and water solubilities (2.05 times the drug substance). 4-MU PC/CNPs significantly improved 4-MU oral bioavailability, representing a promising approach for enhancing drug solubility. This study demonstrates that the targeting parameters of 4-MU PC/CNPs in the liver were all greater than 1, indicating that they specifically targeted the liver, thereby potentially alleviating liver fibrosis.","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":"21 8","pages":"357-368"},"PeriodicalIF":1.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138795730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Short Appraisal of Biomimetic Hydrogels to Improve Penetration of Poorly Permeable Drugs. 提高低渗透性药物渗透的仿生水凝胶的初步评价。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-11-01 Epub Date: 2023-11-21 DOI: 10.1089/adt.2023.077

Dilpreet Singh, Sahil Arora, Vimal Arora

{"title":"A Short Appraisal of Biomimetic Hydrogels to Improve Penetration of Poorly Permeable Drugs.","authors":"Dilpreet Singh, Sahil Arora, Vimal Arora","doi":"10.1089/adt.2023.077","DOIUrl":"10.1089/adt.2023.077","url":null,"abstract":"Effective drug delivery to target sites is critical for achieving desired therapeutic outcomes. However, the poor permeability of certain drugs poses significant challenges in achieving adequate drug concentrations at the desired locations. Biomimetic hydrogels have emerged as a promising approach to enhance the penetration of poorly permeable drugs. These hydrogels, designed to mimic natural biological systems, offer unique properties and functionalities that enable improved drug permeation. In this review, we provide a comprehensive appraisal of the role of biomimetic hydrogels in enhancing drug penetration. We discuss the design principles, properties, and mechanisms by which these hydrogels facilitate drug permeation. Specifically, we explore the applications and benefits of biomimetic hydrogels in controlled drug release, mimicking extracellular matrix microenvironments, promoting cell-mimetic interactions, and enabling targeted drug delivery. Through an examination of key studies and advancements, we highlight the potential of biomimetic hydrogels in enhancing drug penetration and their implications for therapeutic interventions. This review contributes to a deeper understanding of biomimetic hydrogels as a promising strategy for overcoming drug penetration challenges and advancing drug delivery systems, ultimately leading to improved therapeutic efficacy.","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"374-384"},"PeriodicalIF":1.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-Tocopherol Polyethylene Glycol 1000 Succinate-Based Cationic Liposome for the Intracellular Delivery of Doxorubicin in MDA-MB-231 Triple-Negative Breast Cancer Cell Line. α-生育酚聚乙二醇1000琥珀酸基阳离子脂质体用于MDA-MB-231三阴性乳腺癌细胞株的胞内递送阿霉素。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-11-01 Epub Date: 2023-11-21 DOI: 10.1089/adt.2023.067

Pallavi Sandal, Preeti Patel, Dilpreet Singh, Ghanshyam Das Gupta, Balak Das Kurmi

{"title":"α-Tocopherol Polyethylene Glycol 1000 Succinate-Based Cationic Liposome for the Intracellular Delivery of Doxorubicin in MDA-MB-231 Triple-Negative Breast Cancer Cell Line.","authors":"Pallavi Sandal, Preeti Patel, Dilpreet Singh, Ghanshyam Das Gupta, Balak Das Kurmi","doi":"10.1089/adt.2023.067","DOIUrl":"10.1089/adt.2023.067","url":null,"abstract":"Present research work reports the development of doxorubicin (DOX) loaded α-tocopherol polyethylene glycol 1000 succinate (TPGS)-coated cationic liposomes. The developed formulation was evaluated for its anticancer potential and intracellular uptake against the MDA-MB-231 breast cancer cell line. Moreover, hemocompatibility studies were also done on human blood red blood cells for the determination of blood compatibility. The prepared doxorubicin-loaded TPGS liposomes (DOX-LIPO-TPGS) and doxorubicin-loaded cationic liposomes (DOX-LIPO+-TPGS) reveal vesicle size (177.5 ± 2.5 and 201.7 ± 2.3 nm), polydispersity index (0.189 ± 0.01 and 0.218 ± 0.02), zeta potential (-36.9 ± 0.7 and 42 ± 0.9 mv), and % entrapment efficiency (65.88% ± 3.7% and 74.5% ± 3.9%). Furthermore, in vitro, drug release kinetics of the drug alone and drug from formulation shows sustained release behavior of developed formulation with 99.98% in 12 h and 80.98% release of the drug in 72 h, respectively. In addition, cytotoxicity studies and cellular DOX uptake on the MDA-MB-231 breast cancer cell line depict higher cytotoxic and drug uptake potential with better hemocompatibility of DOX-LIPO+-TPGS with respect to DOX. The data from the study revealed that TPGS plays an important role in enhancing the formulation's quality attributes like stability, drug release, cytotoxicity, and hemocompatibility behavior. This may serve that TPGS-coated cationic liposome as a vital candidate for the treatment of cancer and drug delivery in case of breast cancer.","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":" ","pages":"345-356"},"PeriodicalIF":1.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter to the Editor: Repurposing Tafenoquine as a Potential Inhibitor Against Severe Acute Respiratory Syndrome Coronavirus 2 Main Protease via a Fluorescence Polarization Assay. 致编辑的信：通过荧光偏振测定将塔非诺奎重新用作严重急性呼吸综合征冠状病毒2主要蛋白酶的潜在抑制剂。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-11-01 Epub Date: 2023-11-07 DOI: 10.1089/adt.2023.103

Rui Zhang, Haohao Yan, Gangan Yan, Xiaoping Liu, Yunyu Chen

引用次数: 0

Letter to the Editor: Concerns Regarding Ethical Issues in Reviewer Recommendations for Citations. 致编辑的信:对引文审稿人推荐中的伦理问题的关注。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-11-01 Epub Date: 2023-11-15 DOI: 10.1089/adt.2023.117

Rajendra Awasthi

引用次数: 0

Drug Repurposing Patent Applications July-September 2023. 药物再利用专利申请2023年7月至9月。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-11-01 Epub Date: 2023-11-10 DOI: 10.1089/adt.2023.125

Hermann A M Mucke

引用次数: 0

Quality by Design Approach for Optimization of Microbial and pH-Triggered Colon-Targeted Tablet Formulation Using Carboxymethyl Tamarind Gum. 采用羧甲基罗望子胶优化微生物和pH触发结肠靶向片剂配方的设计质量方法。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-10-01 DOI: 10.1089/adt.2023.066

Jaymin Patel, Kaushika Patel, Shreeraj Shah

{"title":"Quality by Design Approach for Optimization of Microbial and pH-Triggered Colon-Targeted Tablet Formulation Using Carboxymethyl Tamarind Gum.","authors":"Jaymin Patel, Kaushika Patel, Shreeraj Shah","doi":"10.1089/adt.2023.066","DOIUrl":"10.1089/adt.2023.066","url":null,"abstract":"ABSTRACT The purpose of this study was to apply the quality by design (QbD) approach in the development of a microbial and pH-triggered colon-targeted budesonide tablet. A retrospective research strategy was used to select various polysaccharide-based natural gums such as tamarind gum, gellan gum, karaya gum, gum ghutti, and khaya gum, which were then evaluated for their effectiveness in microbial degradation and targeting the colon. Viscosity profiles were generated in the presence of a prebiotic culture medium prepared by using the Velgut capsule that mimicked the impact of 4% rat cecal content and helpful in screening of natural polymer. Based on the cumulative drug release data of preliminary batches, carboxymethyl (CM) tamarind gum was identified as a superior and an excellent polymer over the tamarind gum for formulation development. The presence of water as a bridging agent in wet granulation also played an important role in the retardation of drug release. Tablets were supercoated with the enteric polymer, Eudragit S100. The Box-Behnken design was utilized, where the selected independent variables were the proportion of CM tamarind gum, % water proportion, and % weight gain of Eudragit S 100 to optimize the formulation. The optimized design space was generated with the criteria that a drug release should be of less than 5% within the first 2 h, less than 10% within the first 5 h, and more than 70% within the first 8 h, to achieve colon targeting. The optimized batch F3 was found stable as per International Council for Harmonisation guidelines. The roentgenography study for optimized formulation demonstrated that it remained intact for 5 h and, at 7 h, was disseminated completely. CM tamarind gum is efficient for colon targeting, and its proportion in 100 mg along with an enteric coating of 6% led to the optimized formulation.","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":"21 7","pages":"297-308"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjusting the Fitting of Fluorescence-Based Dose-Response Kinase Inhibition Assay to Account for Fluorescent Inhibitors. 调整基于荧光的剂量反应激酶抑制测定的拟合以考虑荧光抑制剂。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-10-01 Epub Date: 2023-09-28 DOI: 10.1089/adt.2023.044

Guillaume A Petit

引用次数: 0

Determination of the Toxicity Preferences of Ocular Drug Delivery System by Comparing Two Different Toxicity Bioassays. 通过比较两种不同的毒性生物测定法测定眼部给药系统的毒性偏好。

IF 1.8 4区医学

Assay and drug development technologies Pub Date : 2023-10-01 DOI: 10.1089/adt.2023.058

Burcu Uner, Meltem Ezgi Durgun, Samet Ozdemir, Cetin Tas, Melike Uner, Yildiz Ozsoy

{"title":"Determination of the Toxicity Preferences of Ocular Drug Delivery System by Comparing Two Different Toxicity Bioassays.","authors":"Burcu Uner, Meltem Ezgi Durgun, Samet Ozdemir, Cetin Tas, Melike Uner, Yildiz Ozsoy","doi":"10.1089/adt.2023.058","DOIUrl":"10.1089/adt.2023.058","url":null,"abstract":"Ocular drug delivery methods are highly favored for boosting bioavailability, patient compliance, and lower adverse effects and dose frequency. In addition to preventing adverse effects from the active ingredient, the parts of drug delivery systems must be nontoxic and nonallergic as well. Mitochondrial toxicity test (MTT) and Hen's egg chorioallantois membrane (HET-CAM) assay are the most often utilized tests based on this dilemma. The toxicity of loteprednol etabonate loaded solid lipid nanoparticles, lipid nanostructured carriers, and nanoemulsion were compared. Oleic acid, Precirol®ATO5, and Pluronic® F68 were used in the preparation. Their toxicities were evaluated by using two different toxicity tests (MTT and HET-CAM). The results suggest that there are no significant differences between the HET-CAM and MTT assays. It is noteworthy that the HET-CAM assay offers a more cost-effective and environmentally friendly alternative to the MTT assay, as it does not require cell culture and generates less toxic waste. This information may be useful to consider when selecting between the two assays.","PeriodicalId":8586,"journal":{"name":"Assay and drug development technologies","volume":"21 7","pages":"337-343"},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0